BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesV0573.Seq (584 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g17900.1 68415.m02073 zinc finger (MYND type) family protein ... 38 0.007 At5g65450.1 68418.m08231 ubiquitin carboxyl-terminal hydrolase f... 32 0.24 At4g31670.1 68417.m04497 ubiquitin carboxyl-terminal hydrolase f... 32 0.32 At4g24560.1 68417.m03520 ubiquitin-specific protease 16, putativ... 31 0.57 At1g17110.1 68414.m02085 ubiquitin-specific protease 15 (UBP15) ... 31 0.75 At2g24640.1 68415.m02943 ubiquitin carboxyl-terminal hydrolase f... 29 1.7 At2g04235.1 68415.m00411 expressed protein weak similarity to ne... 29 2.3 At5g64440.1 68418.m08095 amidase family protein low similarity t... 29 3.0 At2g26430.1 68415.m03171 ania-6a type cyclin (RCY1) nearly ident... 28 5.3 At5g43690.1 68418.m05340 sulfotransferase family protein similar... 27 9.2 At4g25390.1 68417.m03652 protein kinase family protein contains ... 27 9.2 At1g77130.1 68414.m08985 glycogenin glucosyltransferase (glycoge... 27 9.2 >At2g17900.1 68415.m02073 zinc finger (MYND type) family protein / SET domain-containing protein contains Pfam profiles PF00856: SET domain, PF01753: MYND zinc finger (ZnF) domain; confirmed by cDNA (Paola Vittorioso--accession not assigned yet (paola.vittorioso@uniroma1.it) Length = 480 Score = 37.5 bits (83), Expect = 0.007 Identities = 11/25 (44%), Positives = 15/25 (60%) Frame = +3 Query: 363 SRCKTEWYCGRECQVQQWPKHKELC 437 S C+ WYCG CQ +W H++ C Sbjct: 69 SACQVVWYCGSSCQKSEWKLHRDEC 93 >At5g65450.1 68418.m08231 ubiquitin carboxyl-terminal hydrolase family protein / zinc finger (MYND type) family protein similar to ubiquitin-specific protease 16 (UBP16) [Arabidopsis thaliana] GI:11993477; contains Pfam profiles PF00443: Ubiquitin carboxyl-terminal hydrolase, PF01753: MYND finger Length = 731 Score = 32.3 bits (70), Expect = 0.24 Identities = 12/26 (46%), Positives = 17/26 (65%), Gaps = 1/26 (3%) Frame = +3 Query: 363 SRCKTEWYCGRECQVQQWPK-HKELC 437 S+CK+ YC +CQ+ W + HKE C Sbjct: 69 SQCKSVRYCSSKCQILHWRRGHKEEC 94 >At4g31670.1 68417.m04497 ubiquitin carboxyl-terminal hydrolase family protein / zinc finger (MYND type) family protein similar to ubiquitin-specific protease 15 (UBP15) [Arabidopsis thaliana] GI:11993475; contains Pfam profiles PF00443: Ubiquitin carboxyl-terminal hydrolase, PF01753: MYND finger Length = 631 Score = 31.9 bits (69), Expect = 0.32 Identities = 13/29 (44%), Positives = 15/29 (51%), Gaps = 1/29 (3%) Frame = +3 Query: 363 SRCKTEWYCGRECQVQQWPK-HKELCDQY 446 SRCK+ YC ECQ W H+ C Y Sbjct: 73 SRCKSVRYCSAECQRSDWSSGHQRNCRDY 101 >At4g24560.1 68417.m03520 ubiquitin-specific protease 16, putative (UBP16) similar to ubiquitin-specific protease 16 GI:11993477 [Arabidopsis thaliana] Length = 1008 Score = 31.1 bits (67), Expect = 0.57 Identities = 12/26 (46%), Positives = 16/26 (61%), Gaps = 1/26 (3%) Frame = +3 Query: 363 SRCKTEWYCGRECQVQQWPK-HKELC 437 SRCK YC +CQ+ W + HK+ C Sbjct: 86 SRCKAVRYCSGKCQIIHWRQGHKDEC 111 >At1g17110.1 68414.m02085 ubiquitin-specific protease 15 (UBP15) almost identical to ubiquitin-specific protease 15 GI:11993475 [Arabidopsis thaliana], 7 amino acid difference Length = 924 Score = 30.7 bits (66), Expect = 0.75 Identities = 12/26 (46%), Positives = 16/26 (61%), Gaps = 1/26 (3%) Frame = +3 Query: 363 SRCKTEWYCGRECQVQQW-PKHKELC 437 SRCK+ YC +CQ+ W HK+ C Sbjct: 142 SRCKSVRYCSGKCQIIHWRVAHKDEC 167 >At2g24640.1 68415.m02943 ubiquitin carboxyl-terminal hydrolase family protein / zinc finger (MYND type) family protein similar to ubiquitin-specific protease 15 (UBP15) [Arabidopsis thaliana] GI:11993475; contains Pfam profiles PF00443: Ubiquitin carboxyl-terminal hydrolase, PF01753: MYND finger Length = 672 Score = 29.5 bits (63), Expect = 1.7 Identities = 12/26 (46%), Positives = 13/26 (50%), Gaps = 1/26 (3%) Frame = +3 Query: 363 SRCKTEWYCGRECQVQQWPK-HKELC 437 SRCK+ YC CQ W HK C Sbjct: 76 SRCKSVRYCSAACQTSDWKSGHKLKC 101 >At2g04235.1 68415.m00411 expressed protein weak similarity to neurofilament protein (GI:161292) [Loligo pealei]; weak similarity to Glucoamylase S1/S2 precursor (EC 3.2.1.3) (Glucan 1,4-alpha- glucosidase) (1,4-alpha-D-glucan glucohydrolase) (Swiss-Prot:P08640) [Saccharomyces cerevisiae] Length = 1226 Score = 29.1 bits (62), Expect = 2.3 Identities = 23/85 (27%), Positives = 43/85 (50%), Gaps = 2/85 (2%) Frame = +1 Query: 13 ETMLDQIPPLGELKTFLCRLSVGDYSGLHNRTNGVK--NPGCTLIEVVPQIKDAYVKQIH 186 + ++ ++ LGE C+ V +YSG+ N+ +K +P C L+ + D +VK Sbjct: 1012 DILIWKVDSLGEWND--CQSIVLNYSGVFNQRLTLKPGHPSCVLVS--NSLSDTFVKHFP 1067 Query: 187 KRTKSLAKTQLEYFRMDGSEDSRKW 261 + S+A + M +EDSR++ Sbjct: 1068 EMNVSIA-----FNSMFNAEDSRRY 1087 >At5g64440.1 68418.m08095 amidase family protein low similarity to enantiomerase-selective amidase [Rhodococcus sp.] GI:152052; contains Pfam profile PF01425: Amidase Length = 607 Score = 28.7 bits (61), Expect = 3.0 Identities = 13/36 (36%), Positives = 21/36 (58%) Frame = -2 Query: 361 HFLQAFSPHLAHLAPPPSSARAVSLAYESRSFFAIF 254 H + SP L+ L P + + L+Y++R+ FAIF Sbjct: 441 HVISIGSPTLSSLTPYCEAGKNSKLSYDTRTSFAIF 476 >At2g26430.1 68415.m03171 ania-6a type cyclin (RCY1) nearly identical to ania-6a type cyclin [Arabidopsis thaliana] GI:13924511 Length = 416 Score = 27.9 bits (59), Expect = 5.3 Identities = 14/44 (31%), Positives = 24/44 (54%), Gaps = 3/44 (6%) Frame = -1 Query: 272 KFLRHFLESSE-PSMRKYSSWVFANDLVRLCICFTYASFI--CG 150 KF+ ++L + E P + +W AND +R +C + S + CG Sbjct: 156 KFISNYLATLETPPELRQEAWNLANDSLRTTLCVRFRSEVVACG 199 >At5g43690.1 68418.m05340 sulfotransferase family protein similar to steroid sulfotransferase 3 [Brassica napus] GI:3420008, steroid sulfotransferase 1 [Brassica napus] GI:3420004; contains Pfam profile PF00685: Sulfotransferase domain Length = 331 Score = 27.1 bits (57), Expect = 9.2 Identities = 16/55 (29%), Positives = 24/55 (43%) Frame = +1 Query: 34 PPLGELKTFLCRLSVGDYSGLHNRTNGVKNPGCTLIEVVPQIKDAYVKQIHKRTK 198 P L + T RL + LH G+K C ++ + IKD + H R+K Sbjct: 127 PDLTKFSTSSPRL-FSTHMPLHTFKEGLKGSPCKVVYMCRNIKDVLISDWHFRSK 180 >At4g25390.1 68417.m03652 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 651 Score = 27.1 bits (57), Expect = 9.2 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 2/38 (5%) Frame = +1 Query: 160 KDAYVKQIHKRTKSLAKTQLE--YFRMDGSEDSRKWRR 267 KD Y +++ K+ K K LE + DGS +WRR Sbjct: 428 KDEYRRELAKKRKKKKKMTLEAEFCSDDGSSSVSQWRR 465 >At1g77130.1 68414.m08985 glycogenin glucosyltransferase (glycogenin)-related contains similarity to glycogenin-1 from Mus musculus [SP|Q9R062], Rattus norvegicus [SP|O08730], Homo sapiens [SP|P46976] Length = 618 Score = 27.1 bits (57), Expect = 9.2 Identities = 10/24 (41%), Positives = 15/24 (62%) Frame = -1 Query: 269 FLRHFLESSEPSMRKYSSWVFAND 198 FL+HF E EP ++K + +F D Sbjct: 458 FLKHFWEGDEPEIKKMKTSLFGAD 481 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,031,190 Number of Sequences: 28952 Number of extensions: 211101 Number of successful extensions: 581 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 566 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 581 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1151426952 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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