BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesV0527.Seq (648 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_58517| Best HMM Match : Ribosomal_L30_N (HMM E-Value=1.5e-32) 84 8e-17 SB_59006| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.4 SB_32431| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.7 SB_32430| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.7 SB_39493| Best HMM Match : zf-TRAF (HMM E-Value=1.5e-09) 28 7.5 SB_25984| Best HMM Match : ubiquitin (HMM E-Value=0) 28 7.5 SB_24319| Best HMM Match : DUF1279 (HMM E-Value=0.58) 27 9.9 >SB_58517| Best HMM Match : Ribosomal_L30_N (HMM E-Value=1.5e-32) Length = 245 Score = 84.2 bits (199), Expect = 8e-17 Identities = 38/45 (84%), Positives = 42/45 (93%) Frame = +3 Query: 372 EVRKVLQLFRLRQINNGVFVRLNKATVNMLRIAEPYIAWGYPNLK 506 +VRK+LQL RLRQINNGVFVRLNKAT NMLRI +PYIA+GYPNLK Sbjct: 101 KVRKILQLLRLRQINNGVFVRLNKATANMLRIVQPYIAFGYPNLK 145 Score = 72.9 bits (171), Expect = 2e-13 Identities = 33/49 (67%), Positives = 38/49 (77%) Frame = +2 Query: 488 GIPQLKEVRELVYKRGFAKLSGQRIPITSNSIVEKRLHKHNIICVEDLI 634 G P LK VREL+YKRG+ K+ QR+ +T NSIVEK L KH IICVEDLI Sbjct: 140 GYPNLKSVRELIYKRGYGKVDKQRVALTDNSIVEKVLGKHGIICVEDLI 188 Score = 62.1 bits (144), Expect = 4e-10 Identities = 29/49 (59%), Positives = 36/49 (73%) Frame = +1 Query: 253 QERDEIRLARQARNRGNYYVPGEAKLAFVIRIRGINQVSPKSVKFCNCL 399 +E DE+R+ + A+ GN+YVP EA+LAFVIRIRGIN VSPK K L Sbjct: 61 KEVDELRMKKMAKKHGNFYVPPEARLAFVIRIRGINGVSPKVRKILQLL 109 Score = 48.0 bits (109), Expect = 7e-06 Identities = 24/61 (39%), Positives = 35/61 (57%) Frame = +2 Query: 80 EDSKKLPAVPESVLKHXXXXXXXXXXXLQVTLKRRSSAIKKKREIFKRAEQYVKEYRIRN 259 +D K+P VPE++LK + L ++ K++EIFKRAE+YVKEYR + Sbjct: 3 QDRVKVPRVPETLLKKRKSLEQIKAARAKAQLAQKKLQHGKRKEIFKRAEKYVKEYRQKE 62 Query: 260 V 262 V Sbjct: 63 V 63 >SB_59006| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1211 Score = 30.3 bits (65), Expect = 1.4 Identities = 16/46 (34%), Positives = 22/46 (47%) Frame = +2 Query: 278 PDKHAIVATTTFPGKPNWHLSSESVVSTKFHRSP*SSATV*TAPNK 415 PDK A+ TTT P + ++ T + R P S T+ T P K Sbjct: 363 PDKTAVPKTTTAPETTDASKTTVETQQTTYSRDPEISVTISTQPKK 408 >SB_32431| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 322 Score = 28.3 bits (60), Expect = 5.7 Identities = 11/30 (36%), Positives = 16/30 (53%) Frame = -3 Query: 643 ISWNEVLNTDNVVFMEPLLNNAVGSDWYTL 554 + WN L + VV + N AVG +WY + Sbjct: 285 LPWNTALTQELVVKVTSSYNTAVGQNWYEM 314 >SB_32430| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 337 Score = 28.3 bits (60), Expect = 5.7 Identities = 11/30 (36%), Positives = 16/30 (53%) Frame = -3 Query: 643 ISWNEVLNTDNVVFMEPLLNNAVGSDWYTL 554 + WN L + VV + N AVG +WY + Sbjct: 300 LPWNTALTQELVVKVTSSYNTAVGQNWYEM 329 >SB_39493| Best HMM Match : zf-TRAF (HMM E-Value=1.5e-09) Length = 310 Score = 27.9 bits (59), Expect = 7.5 Identities = 11/27 (40%), Positives = 15/27 (55%) Frame = -3 Query: 331 PIWLPRERSSCHDCVLVWLV*FHHVPD 251 P+ P E S C CVL WL ++ P+ Sbjct: 28 PLMAPCEHSYCSACVLGWLTHYNTCPE 54 >SB_25984| Best HMM Match : ubiquitin (HMM E-Value=0) Length = 147 Score = 27.9 bits (59), Expect = 7.5 Identities = 16/51 (31%), Positives = 28/51 (54%), Gaps = 3/51 (5%) Frame = +1 Query: 235 RQGIPHQERDEIRLARQ---ARNRGNYYVPGEAKLAFVIRIRGINQVSPKS 378 ++GIP ++ I +Q R +Y + E+ L V+R+RG Q+S K+ Sbjct: 33 KEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQISVKA 83 >SB_24319| Best HMM Match : DUF1279 (HMM E-Value=0.58) Length = 383 Score = 27.5 bits (58), Expect = 9.9 Identities = 14/39 (35%), Positives = 19/39 (48%) Frame = -3 Query: 298 HDCVLVWLV*FHHVPDAVFLDVLFSPLEDFPLLLDGRRT 182 HD + V+ H P + F D +F L + L L GR T Sbjct: 317 HDALRVYHQIARHQPQSFFYDRVFKQLRELHLRLHGRPT 355 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,801,910 Number of Sequences: 59808 Number of extensions: 375059 Number of successful extensions: 778 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 740 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 778 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1645141000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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