Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesV0518.Seq
(648 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g52240.1 68418.m06484 cytochrome b5 domain-containing protein... 87 9e-18
At3g48890.1 68416.m05341 cytochrome b5 domain-containing protein... 84 7e-17
At2g24940.1 68415.m02982 cytochrome b5 domain-containing protein... 81 5e-16
At4g14965.1 68417.m02300 cytochrome b5 domain-containing protein... 63 1e-10
At4g16790.1 68417.m02536 hydroxyproline-rich glycoprotein family... 33 0.16
At3g56140.1 68416.m06240 expressed protein At2g40400 - Arabidops... 33 0.16
At5g36060.1 68418.m04344 hypothetical protein 30 1.5
At1g76240.1 68414.m08853 expressed protein contains Pfam profile... 29 2.0
At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila... 29 2.7
At5g14920.1 68418.m01750 gibberellin-regulated family protein si... 29 3.5
At5g56890.1 68418.m07099 protein kinase family protein contains ... 28 6.1
At3g53570.3 68416.m05915 protein kinase (AFC1) (AME2) identical ... 28 6.1
At3g53570.2 68416.m05916 protein kinase (AFC1) (AME2) identical ... 28 6.1
At3g53570.1 68416.m05917 protein kinase (AFC1) (AME2) identical ... 28 6.1
At1g77510.1 68414.m09026 protein disulfide isomerase, putative s... 28 6.1
At5g38560.1 68418.m04662 protein kinase family protein contains ... 27 8.1
At3g52220.1 68416.m05737 expressed protein 27 8.1
>At5g52240.1 68418.m06484 cytochrome b5 domain-containing protein
similar to SP|P70580 Membrane associated progesterone
receptor component 1 {Rattus norvegicus}; contains Pfam
profile PF00173: Heme/Steroid binding domain
Length = 220
Score = 87.0 bits (206), Expect = 9e-18
Identities = 44/102 (43%), Positives = 62/102 (60%), Gaps = 1/102 (0%)
Frame = +1
Query: 268 DMTAVELRQYDGTQEGGRVLMAVNGWIFDVTRGNRFYGPGGPYAVFGGRDATRGLATFSV 447
++T EL+QYDG+ +LMA+ I+DVT+ FYGPGGPYA+F G+DA+R LA S
Sbjct: 74 EITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFAGKDASRALAKMSF 133
Query: 448 TAPEKDYD-DLSDLNSMEMGIMREWEEQFRENYDLVXRLSEA 570
EKD D+S L E+ +++WE +F Y V + A
Sbjct: 134 --EEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVKVA 173
>At3g48890.1 68416.m05341 cytochrome b5 domain-containing protein
similar to SP|O00264 Membrane associated progesterone
receptor component (mPR) {Homo sapiens}; contains Pfam
profile PF00173: Heme/Steroid binding domain
Length = 233
Score = 84.2 bits (199), Expect = 7e-17
Identities = 38/92 (41%), Positives = 58/92 (63%)
Frame = +1
Query: 268 DMTAVELRQYDGTQEGGRVLMAVNGWIFDVTRGNRFYGPGGPYAVFGGRDATRGLATFSV 447
++T EL+ YDG+ +LMA+ G I+DV++ FYGPGGPYA+F G+DA+R LA S
Sbjct: 70 EITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSF 129
Query: 448 TAPEKDYDDLSDLNSMEMGIMREWEEQFRENY 543
+ D+S L + E+ +++WE +F Y
Sbjct: 130 EDQDLT-GDISGLGAFELEALQDWEYKFMSKY 160
>At2g24940.1 68415.m02982 cytochrome b5 domain-containing protein
similar to SP|P70580 Membrane associated progesterone
receptor component 1 {Rattus norvegicus}; contains Pfam
profile PF00173: Heme/Steroid binding domain
Length = 100
Score = 81.4 bits (192), Expect = 5e-16
Identities = 39/99 (39%), Positives = 57/99 (57%), Gaps = 1/99 (1%)
Frame = +1
Query: 268 DMTAVELRQYDGTQEGGRVLMAVNGWIFDVTRGNRFYGPGGPYAVFGGRDATRGLATFSV 447
+ TA +L QY+GT E + +A+ G +FDVT G FYG GG Y++F G+DA+R L S
Sbjct: 2 EFTAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSK 61
Query: 448 TAPEKDYD-DLSDLNSMEMGIMREWEEQFRENYDLVXRL 561
E+D L L E+ + +WE +F Y +V R+
Sbjct: 62 N--EEDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRV 98
>At4g14965.1 68417.m02300 cytochrome b5 domain-containing protein
similar to SP|O15173 Membrane associated progesterone
receptor component 2 (Steroid receptor protein DG6)
{Homo sapiens}; contains Pfam profile PF00173:
Heme/Steroid binding domain
Length = 245
Score = 63.3 bits (147), Expect = 1e-10
Identities = 35/96 (36%), Positives = 52/96 (54%)
Frame = +1
Query: 274 TAVELRQYDGTQEGGRVLMAVNGWIFDVTRGNRFYGPGGPYAVFGGRDATRGLATFSVTA 453
+A EL Y+GT E +L+ + G +FDVT+G YG GG Y F GRDA+R + + T
Sbjct: 43 SAEELALYNGTDETLPILLGILGSVFDVTKGKFHYGSGGGYNHFAGRDASRAFVSGNFTG 102
Query: 454 PEKDYDDLSDLNSMEMGIMREWEEQFRENYDLVXRL 561
+ D L L+S E+ + +W + Y V +L
Sbjct: 103 -DGLTDSLQGLSSSEVKSIVDWRGFYSRTYTPVGKL 137
>At4g16790.1 68417.m02536 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 473
Score = 33.1 bits (72), Expect = 0.16
Identities = 15/52 (28%), Positives = 26/52 (50%)
Frame = -2
Query: 434 ASPLVASLPPKTAYGPPGP*NRFPLVTSNIHPFTAINTLPPSCVPSYCRSST 279
+ PL+ +L P +++ P N P + S HP PP +P++ SS+
Sbjct: 249 SQPLIKNLTPPSSFSSPRKSNPIPNLASEFHPSPPPPPPPPPPLPAFYNSSS 300
>At3g56140.1 68416.m06240 expressed protein At2g40400 - Arabidopsis
thaliana, EMBL:AC007020
Length = 745
Score = 33.1 bits (72), Expect = 0.16
Identities = 24/66 (36%), Positives = 31/66 (46%)
Frame = -2
Query: 524 SSHSLMIPISIELRSLKSS*SFSGAVTENVASPLVASLPPKTAYGPPGP*NRFPLVTSNI 345
S+ SL + S+ L S +SS S VT S P T PP P N+ +TS I
Sbjct: 77 SAASLFLKPSVSLASEESS---SATVTSPAESAAPPPPPATTTPSPPPPVNKEETITSRI 133
Query: 344 HPFTAI 327
+ TAI
Sbjct: 134 YDATAI 139
>At5g36060.1 68418.m04344 hypothetical protein
Length = 165
Score = 29.9 bits (64), Expect = 1.5
Identities = 19/58 (32%), Positives = 26/58 (44%)
Frame = +3
Query: 258 IAKRYDRSRTTAVRRDTGGWQSVDGCEWVDIRCD*RESVLRSRWAICCFWRKRCHKRT 431
+A+ Y S + GGW +DG W R RES+ R C + R HKR+
Sbjct: 110 VAECYRSSLWMEPYGEDGGWVQIDGAYWAQPRV--RESIPR-----CSRCKSRAHKRS 160
>At1g76240.1 68414.m08853 expressed protein contains Pfam profile
PF03087: Arabidopsis protein of unknown function
Length = 308
Score = 29.5 bits (63), Expect = 2.0
Identities = 18/44 (40%), Positives = 23/44 (52%)
Frame = -2
Query: 560 SRXTRS*FSLNCSSHSLMIPISIELRSLKSS*SFSGAVTENVAS 429
S TRS SL C SH L+ ++ E+ LKS SF+G S
Sbjct: 28 SHHTRS-ISLPCRSHPLISHVNHEISQLKSWFSFAGETHSRTTS 70
>At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar
to SP|P35207 Antiviral protein SKI2 {Saccharomyces
cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH
box helicase, PF00271: Helicase conserved C-terminal
domain
Length = 1347
Score = 29.1 bits (62), Expect = 2.7
Identities = 21/60 (35%), Positives = 27/60 (45%), Gaps = 2/60 (3%)
Frame = +1
Query: 388 GPYAVFGGRDATRGL-ATFSVTAPEKD-YDDLSDLNSMEMGIMREWEEQFRENYDLVXRL 561
G Y GR RGL T +V +D D SDL + +G E QFR Y ++ L
Sbjct: 769 GEYTQMAGRAGRRGLDKTGTVVVMCRDEVPDESDLRRVIVGSATRLESQFRLTYIMILHL 828
>At5g14920.1 68418.m01750 gibberellin-regulated family protein
similar to SP|P46689 Gibberellin-regulated protein 1
precursor {Arabidopsis thaliana}; contains Pfam profile
PF02704: Gibberellin regulated protein
Length = 275
Score = 28.7 bits (61), Expect = 3.5
Identities = 18/50 (36%), Positives = 24/50 (48%), Gaps = 1/50 (2%)
Frame = -2
Query: 446 TENVASPLVASLPPKTAYGPPGP*NRFP-LVTSNIHPFTAINTLPPSCVP 300
T + SP A+ PP A PP P + P L T+ I P T + P +P
Sbjct: 35 TPTLPSPSPATKPPSPALKPPTPSYKPPTLPTTPIKPPTTKPPVKPPTIP 84
>At5g56890.1 68418.m07099 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 1113
Score = 27.9 bits (59), Expect = 6.1
Identities = 18/42 (42%), Positives = 20/42 (47%), Gaps = 2/42 (4%)
Frame = -2
Query: 461 FSGAVTENVASPLVASLP--PKTAYGPPGP*NRFPLVTSNIH 342
FSG VT S V+ +P P A PP P N P SN H
Sbjct: 145 FSGRVTPAPVSSPVSDIPPIPSVALPPPTPSNVPPRNASNNH 186
>At3g53570.3 68416.m05915 protein kinase (AFC1) (AME2) identical to
protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana]
Length = 453
Score = 27.9 bits (59), Expect = 6.1
Identities = 18/55 (32%), Positives = 26/55 (47%), Gaps = 3/55 (5%)
Frame = -2
Query: 449 VTENVASPLVASLPPKTAYGPP---GP*NRFPLVTSNIHPFTAINTLPPSCVPSY 294
+T + A PL+ PP T + PP GP LV + ++P N LP P +
Sbjct: 26 LTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNFVYPNMFYNGLPRQGSPPW 80
>At3g53570.2 68416.m05916 protein kinase (AFC1) (AME2) identical to
protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana]
Length = 467
Score = 27.9 bits (59), Expect = 6.1
Identities = 18/55 (32%), Positives = 26/55 (47%), Gaps = 3/55 (5%)
Frame = -2
Query: 449 VTENVASPLVASLPPKTAYGPP---GP*NRFPLVTSNIHPFTAINTLPPSCVPSY 294
+T + A PL+ PP T + PP GP LV + ++P N LP P +
Sbjct: 40 LTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNFVYPNMFYNGLPRQGSPPW 94
>At3g53570.1 68416.m05917 protein kinase (AFC1) (AME2) identical to
protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana]
Length = 467
Score = 27.9 bits (59), Expect = 6.1
Identities = 18/55 (32%), Positives = 26/55 (47%), Gaps = 3/55 (5%)
Frame = -2
Query: 449 VTENVASPLVASLPPKTAYGPP---GP*NRFPLVTSNIHPFTAINTLPPSCVPSY 294
+T + A PL+ PP T + PP GP LV + ++P N LP P +
Sbjct: 40 LTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNFVYPNMFYNGLPRQGSPPW 94
>At1g77510.1 68414.m09026 protein disulfide isomerase, putative
similar to protein disulfide isomerase precursor
GB:P29828 GI:4704766 [Medicago sativa]; Pfam HMM hit:
PF00085 Thioredoxins
Length = 508
Score = 27.9 bits (59), Expect = 6.1
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = +2
Query: 125 ETLKTLFCQPFIYFVFVSTFVILYKFYYKYKTHCSKVAPL 244
E +K + + VF S +L +FY + HC K+AP+
Sbjct: 372 EPVKVVVAESLDDIVFKSGKNVLIEFYAPWCGHCQKLAPI 411
>At5g38560.1 68418.m04662 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 681
Score = 27.5 bits (58), Expect = 8.1
Identities = 24/72 (33%), Positives = 31/72 (43%)
Frame = -2
Query: 515 SLMIPISIELRSLKSS*SFSGAVTENVASPLVASLPPKTAYGPPGP*NRFPLVTSNIHPF 336
SL+ P+ I S SS S + A P S PP PP P P V S+ P
Sbjct: 2 SLVPPLPI--LSPPSSNSSTTAPPPLQTQPTTPSAPPPVT-PPPSPPQSPPPVVSSSPPP 58
Query: 335 TAINTLPPSCVP 300
+++ PPS P
Sbjct: 59 PVVSSPPPSSSP 70
>At3g52220.1 68416.m05737 expressed protein
Length = 237
Score = 27.5 bits (58), Expect = 8.1
Identities = 12/45 (26%), Positives = 23/45 (51%), Gaps = 3/45 (6%)
Frame = +2
Query: 518 GRNNLERIMIWSVDFL---RPGEEPRNYSDEEPEEADTSALPTTT 643
G N + + + + + RP E+P + + E+AD++ LP T
Sbjct: 118 GAGNADAVWVHGLGYAKAPRPWEDPSTLASSQKEDADSARLPADT 162
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,884,025
Number of Sequences: 28952
Number of extensions: 327726
Number of successful extensions: 1066
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 993
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1063
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -