BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesV0513.Seq (299 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g52460.1 68418.m06509 F-box family protein contains F-box dom... 28 1.0 At4g34910.1 68417.m04950 DEAD/DEAH box helicase, putative (RH16)... 27 1.8 At1g54920.2 68414.m06269 expressed protein 26 5.4 At5g06600.2 68418.m00746 ubiquitin-specific protease 12 (UBP12) ... 25 7.1 At5g06600.1 68418.m00745 ubiquitin-specific protease 12 (UBP12) ... 25 7.1 At5g06590.1 68418.m00744 expressed protein 25 7.1 At4g11130.1 68417.m01805 RNA-dependent RNA polymerase, putative ... 25 7.1 At1g48310.1 68414.m05396 SNF2 domain-containing protein / helica... 25 7.1 At1g31140.1 68414.m03810 MADS-box protein (AGL63) similar to gb|... 25 7.1 At3g26000.1 68416.m03239 F-box family protein contains Pfam PF00... 25 9.4 At3g11910.1 68416.m01460 ubiquitin-specific protease, putative s... 25 9.4 >At5g52460.1 68418.m06509 F-box family protein contains F-box domain Pfam:PF00646 Length = 369 Score = 28.3 bits (60), Expect = 1.0 Identities = 24/64 (37%), Positives = 29/64 (45%), Gaps = 1/64 (1%) Frame = +1 Query: 37 LLLNKRNSPTLEHALAAITDCVKLDTGCVRKVFTSSGTQVTALHQFFDEEDVFFAY-GNE 213 L+LN NSP L AIT + +D T+ GT L DEE V+ Y GNE Sbjct: 247 LILN--NSPKLR--FLAITSVMDIDPEISHCHGTNQGTVPRCLSAHLDEEFVWHGYRGNE 302 Query: 214 RVNQ 225 Q Sbjct: 303 EETQ 306 >At4g34910.1 68417.m04950 DEAD/DEAH box helicase, putative (RH16) identical to cDNA DEAD box RNA helicase, RH16 GI:3776006 Length = 626 Score = 27.5 bits (58), Expect = 1.8 Identities = 18/81 (22%), Positives = 38/81 (46%), Gaps = 11/81 (13%) Frame = +1 Query: 4 T*IKTTFQVCRL------LLNKRNSPTLEHALAAITDCVKLDTGCVRKVFTSSGTQVTAL 165 T + + ++CR+ L + ++ + +ALA + + + C+ K F + + TA+ Sbjct: 138 TEVSSLIELCRVQLKAVQLTSSMSASDMRNALAGLPEILVSTPACIPKCFAAGVLEPTAV 197 Query: 166 HQ-----FFDEEDVFFAYGNE 213 + DE D+ +YG E Sbjct: 198 SESLSILVLDEADLLLSYGYE 218 >At1g54920.2 68414.m06269 expressed protein Length = 890 Score = 25.8 bits (54), Expect = 5.4 Identities = 16/54 (29%), Positives = 27/54 (50%), Gaps = 5/54 (9%) Frame = +1 Query: 58 SPTLEHALAAITDCVK--LDTGCVRKVFTS---SGTQVTALHQFFDEEDVFFAY 204 +PT + LA + +C+K L +G V + S S Q+ ++ +E D F Y Sbjct: 775 APTSSNVLAVLDECLKLWLSSGLVEALLNSHDDSADQLLESIKYINEVDAFTLY 828 >At5g06600.2 68418.m00746 ubiquitin-specific protease 12 (UBP12) almost identical to ubiquitin-specific protease 12 GI:11993471 [Arabidopsis thaliana], one amino acid difference Length = 1115 Score = 25.4 bits (53), Expect = 7.1 Identities = 12/33 (36%), Positives = 20/33 (60%) Frame = +1 Query: 187 DVFFAYGNERVNQEDFELEFEEKKPYPSAEKLP 285 D ++ + +ERV +ED + EE+ Y E+LP Sbjct: 465 DQWYKFDDERVTKEDLKRALEEQ--YGGEEELP 495 >At5g06600.1 68418.m00745 ubiquitin-specific protease 12 (UBP12) almost identical to ubiquitin-specific protease 12 GI:11993471 [Arabidopsis thaliana], one amino acid difference Length = 1116 Score = 25.4 bits (53), Expect = 7.1 Identities = 12/33 (36%), Positives = 20/33 (60%) Frame = +1 Query: 187 DVFFAYGNERVNQEDFELEFEEKKPYPSAEKLP 285 D ++ + +ERV +ED + EE+ Y E+LP Sbjct: 466 DQWYKFDDERVTKEDLKRALEEQ--YGGEEELP 496 >At5g06590.1 68418.m00744 expressed protein Length = 314 Score = 25.4 bits (53), Expect = 7.1 Identities = 11/22 (50%), Positives = 14/22 (63%) Frame = +1 Query: 178 DEEDVFFAYGNERVNQEDFELE 243 DEE VF A+G+E D +LE Sbjct: 113 DEESVFGAFGHEASKHSDDDLE 134 >At4g11130.1 68417.m01805 RNA-dependent RNA polymerase, putative similar to RNA-directed RNA polymerase [Lycopersicon esculentum] gi|4038592|emb|CAA71421 Length = 1133 Score = 25.4 bits (53), Expect = 7.1 Identities = 11/37 (29%), Positives = 18/37 (48%), Gaps = 2/37 (5%) Frame = +3 Query: 99 RETRYGMCKESFHIVW--DSSYSSASILRRGRCVFCI 203 + RY CKE F +W + +S + + C FC+ Sbjct: 204 KSDRYRFCKEDFDFMWIRTTDFSGSKSIGTSTC-FCL 239 >At1g48310.1 68414.m05396 SNF2 domain-containing protein / helicase domain-containing protein contains similarity to DNA-dependent ATPase A GI:6651385 from [Bos taurus]}; contains PFam profiles PF00271: Helicase conserved C-terminal domain, PF00176: SNF2 Length = 673 Score = 25.4 bits (53), Expect = 7.1 Identities = 16/63 (25%), Positives = 32/63 (50%), Gaps = 2/63 (3%) Frame = +1 Query: 10 IKTTFQVCRLLLNKRNSPTLEHALAAITDCVK--LDTGCVRKVFTSSGTQVTALHQFFDE 183 IK+ + + L+NK + + + A+ D ++ ++ GC VF + + LHQF + Sbjct: 447 IKSLKFIEKNLINKIYTDSAVAKIPAVLDYLENVIEAGCKFLVFAHHQSMLEELHQFLKK 506 Query: 184 EDV 192 + V Sbjct: 507 KKV 509 >At1g31140.1 68414.m03810 MADS-box protein (AGL63) similar to gb|Y15008 M79 protein (MADS box) from oryza sativa and contains SRF transcription factor domain PF|00319 Length = 213 Score = 25.4 bits (53), Expect = 7.1 Identities = 10/35 (28%), Positives = 17/35 (48%) Frame = +1 Query: 28 VCRLLLNKRNSPTLEHALAAITDCVKLDTGCVRKV 132 + R K T EH+ + +DCVK +R++ Sbjct: 68 IMRYQKEKEGQTTAEHSFHSCSDCVKTKESMMREI 102 >At3g26000.1 68416.m03239 F-box family protein contains Pfam PF00646: F-box domain; similar to SKP1 interacting partner 2 (SKIP2) TIGR_Ath1:At5g67250 Length = 453 Score = 25.0 bits (52), Expect = 9.4 Identities = 12/42 (28%), Positives = 18/42 (42%) Frame = -3 Query: 141 RCENFPYTSRIEFHAIRDRSQGMLECRAITFIQQ*PTHLKRC 16 +C+NF + + +G ECRA + Q H RC Sbjct: 384 KCQNFKLVYDCPAEDCKGKKKGSEECRACSLCIQRCYHCGRC 425 >At3g11910.1 68416.m01460 ubiquitin-specific protease, putative strong similarity to ubiquitin-specific protease 12 (UBP12) [Arabidopsis thaliana] GI:11993471; contains Pfam profiles PF00443: Ubiquitin carboxyl-terminal hydrolase, PF00917: MATH domain Length = 1115 Score = 25.0 bits (52), Expect = 9.4 Identities = 12/33 (36%), Positives = 20/33 (60%) Frame = +1 Query: 187 DVFFAYGNERVNQEDFELEFEEKKPYPSAEKLP 285 D ++ + +ERV +ED + EE+ Y E+LP Sbjct: 465 DQWYKFDDERVTKEDVKRALEEQ--YGGEEELP 495 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 6,321,107 Number of Sequences: 28952 Number of extensions: 114136 Number of successful extensions: 333 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 329 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 333 length of database: 12,070,560 effective HSP length: 70 effective length of database: 10,043,920 effective search space used: 291273680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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