Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesV0505.Seq
(597 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC31H12.07 |sec231|sec23a, SPCC5E4.01|GTPase activating protei... 94 1e-20
SPBC776.04 |sec2302|sec23-b|GTPase activating protein Sec23b |Sc... 83 4e-17
SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo... 38 0.001
SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces p... 28 1.2
SPAC26F1.08c |||conserved protein|Schizosaccharomyces pombe|chr ... 27 1.6
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 27 2.7
SPBC13E7.06 |msd1|mug172|spindle pole body protein Msd1|Schizosa... 25 8.4
>SPCC31H12.07 |sec231|sec23a, SPCC5E4.01|GTPase activating protein
Sec23a|Schizosaccharomyces pombe|chr 3|||Manual
Length = 759
Score = 94.3 bits (224), Expect = 1e-20
Identities = 39/66 (59%), Positives = 45/66 (68%)
Frame = +1
Query: 256 PLKERPDLPPIQYEPVLCTRNTCRAILNPMCQVDYRAKLWVCNFCFQRNPFPPQYAAISE 435
PL ERPDLPP+ YEPV C + C+A+LNP C +D RAK W+C FC QRN PPQY IS
Sbjct: 39 PLNERPDLPPVLYEPVTC-KAPCKAVLNPYCHIDTRAKFWICPFCLQRNMLPPQYKDISN 97
Query: 436 QHQPAE 453
P E
Sbjct: 98 TSLPIE 103
Score = 64.1 bits (149), Expect = 1e-11
Identities = 48/146 (32%), Positives = 68/146 (46%), Gaps = 1/146 (0%)
Frame = +2
Query: 152 QQNEDRDGIRFTWNVWPSSRIEATRLVVPLACLYHRLRSAPTYHRYSTSLYSVHEIHAEL 331
++ E+RDG+RFTWNV+PS+RIE++R +VP+A +Y L P + +
Sbjct: 4 EEIEERDGVRFTWNVFPSTRIESSRTIVPIASIYKPLNERPDLPPVLYEPVTCKAPCKAV 63
Query: 332 Y*TQCARWITELNYGSATFASNETHFHLNMLLYQNSINLQKLIPNFSTIEYTINASNRLG 511
C T + F N+ +L+P +STIEYT+ +L
Sbjct: 64 LNPYC-HIDTRAKFWICPFCLQRNMLPPQYKDISNTSLPIELLPEYSTIEYTLPRPPQLT 122
Query: 512 PKSTCLF-VDTWLDEEELWGTSKDSL 586
P LF VD DEE L KDSL
Sbjct: 123 P--VFLFVVDVCQDEENLQAL-KDSL 145
>SPBC776.04 |sec2302|sec23-b|GTPase activating protein Sec23b
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 765
Score = 82.6 bits (195), Expect = 4e-17
Identities = 36/66 (54%), Positives = 42/66 (63%)
Frame = +1
Query: 256 PLKERPDLPPIQYEPVLCTRNTCRAILNPMCQVDYRAKLWVCNFCFQRNPFPPQYAAISE 435
PL E + QY+PV C R CRA+LNP C VD RA+ W+C FCFQRNP P QY+ IS
Sbjct: 39 PLHENERMTIEQYDPVAC-RAPCRAVLNPYCHVDLRARFWICPFCFQRNPLPAQYSDISS 97
Query: 436 QHQPAE 453
P E
Sbjct: 98 NSLPLE 103
Score = 38.7 bits (86), Expect = 6e-04
Identities = 14/31 (45%), Positives = 22/31 (70%)
Frame = +2
Query: 161 EDRDGIRFTWNVWPSSRIEATRLVVPLACLY 253
ED+DGIR +WN + ++ E R V+P+A +Y
Sbjct: 7 EDQDGIRLSWNTFSATPAENARAVIPIAAMY 37
>SPBC4.03c |||COPII-coated vesicle component Sfb3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 37.9 bits (84), Expect = 0.001
Identities = 21/63 (33%), Positives = 27/63 (42%), Gaps = 7/63 (11%)
Frame = +1
Query: 241 GLLVPPLKE-RPDLPPIQY------EPVLCTRNTCRAILNPMCQVDYRAKLWVCNFCFQR 399
G++V P E RPD P+ P C + CR +NP Q + W CN C
Sbjct: 187 GMIVQPFAELRPDEAPVPVVDNTNSNPPRCKK--CRGYINPFIQFTMASSKWTCNLCGSE 244
Query: 400 NPF 408
N F
Sbjct: 245 NSF 247
>SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 926
Score = 27.9 bits (59), Expect = 1.2
Identities = 17/64 (26%), Positives = 25/64 (39%), Gaps = 3/64 (4%)
Frame = +1
Query: 238 FGLLVPP---LKERPDLPPIQYEPVLCTRNTCRAILNPMCQVDYRAKLWVCNFCFQRNPF 408
F L++ P L E D P+ + ++ CR +NP + CN C N
Sbjct: 228 FALVIRPYTSLVEEDDPVPVVTDTIISRCRRCRMYINPFSIFIDNGHRYRCNSCGIVNEV 287
Query: 409 PPQY 420
P Y
Sbjct: 288 PQSY 291
>SPAC26F1.08c |||conserved protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 977
Score = 27.5 bits (58), Expect = 1.6
Identities = 18/67 (26%), Positives = 28/67 (41%)
Frame = +2
Query: 251 YHRLRSAPTYHRYSTSLYSVHEIHAELY*TQCARWITELNYGSATFASNETHFHLNMLLY 430
+H+LR + +YS YS+ + L+ QC I L +F FH
Sbjct: 9 FHKLRKNEHFLKYSAIFYSLLKPVVALFACQCLVLIRPLARKIGSFPFLILTFHTLFYPI 68
Query: 431 QNSINLQ 451
QN++ Q
Sbjct: 69 QNTVGAQ 75
>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 26.6 bits (56), Expect = 2.7
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = -2
Query: 593 VCKGNLWKSPKVLPHLTKYQQTSR 522
VCK +WKS ++ L KYQ+T R
Sbjct: 225 VCK--IWKSDEINEVLNKYQETQR 246
>SPBC13E7.06 |msd1|mug172|spindle pole body protein
Msd1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 331
Score = 25.0 bits (52), Expect = 8.4
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = -1
Query: 150 MNSSYVAISPIKKSCGEPVINT*ANFLNHN 61
+NS + PIK++ P++ A+FL N
Sbjct: 171 LNSMLIVKEPIKQNTNAPILPETASFLQQN 200
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,734,303
Number of Sequences: 5004
Number of extensions: 59298
Number of successful extensions: 152
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 260219058
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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