BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesV0447.Seq (648 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g47880.1 68418.m05915 eukaryotic peptide chain release factor... 137 7e-33 At3g26618.1 68416.m03325 eukaryotic release factor 1 family prot... 133 1e-31 At1g12920.1 68414.m01500 eukaryotic release factor 1 family prot... 130 1e-30 At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains ... 29 2.0 At5g56240.1 68418.m07018 expressed protein 29 2.7 At4g14480.1 68417.m02233 protein kinase family protein contains ... 29 3.5 At3g59610.1 68416.m06651 F-box family protein / jacalin lectin f... 28 6.1 >At5g47880.1 68418.m05915 eukaryotic peptide chain release factor subunit 1-1 (ERF1-1) identical to SP|Q39097 Eukaryotic peptide chain release factor subunit 1-1 (eRF1-1) (Eukaryotic release factor 1-1) (Omnipotent suppressor protein 1 homolog 1) (SUP1 homolog 1) {Arabidopsis thaliana}, eukaryotic release factor 1 homolog GI:1155261 from [Arabidopsis thaliana]; contains Pfam profiles: PF03463 eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1 domain 3 Length = 436 Score = 137 bits (331), Expect = 7e-33 Identities = 70/101 (69%), Positives = 82/101 (81%), Gaps = 1/101 (0%) Frame = +1 Query: 337 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTVL- 513 M +++ D+N+EIWKIKKLIKSLE ARGNGTSMISLI+PP+DQ+SRV+KML DE+GT Sbjct: 1 MGDKNDDDKNIEIWKIKKLIKSLEAARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 60 Query: 514 ISSHV*IVSPVLGAITSVQHRLKLYTKVPPNGLVISXGTIV 636 I S V S VLGAITS Q RLKLY +VPPNGLV+ GTIV Sbjct: 61 IKSRVNRQS-VLGAITSAQQRLKLYNRVPPNGLVLYTGTIV 100 >At3g26618.1 68416.m03325 eukaryotic release factor 1 family protein / eRF1 family protein contains Pfam profiles: PF03463 eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1 domain 3 Length = 435 Score = 133 bits (321), Expect = 1e-31 Identities = 68/98 (69%), Positives = 79/98 (80%), Gaps = 1/98 (1%) Frame = +1 Query: 346 ESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTVL-ISS 522 + +D+N+EIWKIKKLIK LE ARGNGTSMISLI+PP+DQ++RV+KMLADE+GT I S Sbjct: 3 DQESDKNIEIWKIKKLIKGLETARGNGTSMISLIMPPRDQVARVTKMLADEYGTASNIKS 62 Query: 523 HV*IVSPVLGAITSVQHRLKLYTKVPPNGLVISXGTIV 636 V S VL AITS Q RLKLY KVPPNGLV+ GTIV Sbjct: 63 RVNRQS-VLSAITSAQQRLKLYNKVPPNGLVLYTGTIV 99 >At1g12920.1 68414.m01500 eukaryotic release factor 1 family protein / eRF1 family protein contains Pfam profiles: PF03463 eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1 domain 3 Length = 434 Score = 130 bits (313), Expect = 1e-30 Identities = 71/101 (70%), Positives = 79/101 (78%), Gaps = 1/101 (0%) Frame = +1 Query: 337 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTVL- 513 M+EE AD N+EIWKIKKLIK LE ARGNGTSMISLI+PP+DQ+SRV+KML DE+GT Sbjct: 1 MAEE--ADTNIEIWKIKKLIKGLESARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 58 Query: 514 ISSHV*IVSPVLGAITSVQHRLKLYTKVPPNGLVISXGTIV 636 I S V S VL AITS Q RLKLY KVP NGLV+ GTIV Sbjct: 59 IKSRVNRQS-VLSAITSAQQRLKLYNKVPTNGLVLYTGTIV 98 >At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains similarity to NAD kinase [Homo sapiens] gi|20070086|gb|AAM01195; contains Pfam domain, PF01513: ATP-NAD kinase Length = 985 Score = 29.5 bits (63), Expect = 2.0 Identities = 12/26 (46%), Positives = 16/26 (61%) Frame = +1 Query: 397 KSLEMARGNGTSMISLIIPPKDQISR 474 KS+ RGNG S + +PP D +SR Sbjct: 561 KSISDNRGNGFSAAPIAVPPSDNLSR 586 >At5g56240.1 68418.m07018 expressed protein Length = 986 Score = 29.1 bits (62), Expect = 2.7 Identities = 17/48 (35%), Positives = 24/48 (50%) Frame = +3 Query: 348 IVGRSKCRNMEDKETYKEFGNGQRKWNIDDIPDHSTXGSNLESIKDVG 491 I G++ C+ ED+ E G G +D IP GS L S +D+G Sbjct: 668 IYGQTSCK--EDERFDSEVGQGSLFVEVDTIPIPGPPGSFLPSPRDMG 713 >At4g14480.1 68417.m02233 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 487 Score = 28.7 bits (61), Expect = 3.5 Identities = 16/54 (29%), Positives = 30/54 (55%) Frame = +1 Query: 328 RNKMSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKML 489 +N+ E D+++ + K+K L KSLE R + +I + K++ SR ++L Sbjct: 407 KNQEGEVVGFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKEEKSREEELL 460 >At3g59610.1 68416.m06651 F-box family protein / jacalin lectin family protein contains Pfam profile PF01419: Jacalin-like lectin domain Length = 521 Score = 27.9 bits (59), Expect = 6.1 Identities = 12/45 (26%), Positives = 25/45 (55%) Frame = +1 Query: 337 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQIS 471 + +E RN+EIW K+ IK+ + +++S+++P +S Sbjct: 251 LEQEIYNTRNIEIWVTKETIKNGDGEAVEWVNLMSVLVPEWSSLS 295 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,264,894 Number of Sequences: 28952 Number of extensions: 290708 Number of successful extensions: 675 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 652 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 669 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1344285648 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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