BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesV0429.Seq (844 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 156 3e-39 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 109 5e-25 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 108 8e-25 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 85 2e-17 SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 29 0.82 SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase comple... 29 0.82 SPCC2H8.02 |||inorganic phosphate transporter|Schizosaccharomyce... 28 1.9 SPBC2A9.03 |||conserved protein |Schizosaccharomyces pombe|chr 2... 26 5.8 SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 s... 26 5.8 SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr 3... 26 5.8 SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|ch... 26 7.7 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 7.7 SPBC1861.09 |ppk22||serine/threonine protein kinase Ppk22 |Schiz... 26 7.7 SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|ch... 26 7.7 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 156 bits (379), Expect = 3e-39 Identities = 69/87 (79%), Positives = 78/87 (89%) Frame = +2 Query: 248 GQDVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSEAGNNWAKGHYTEGAELVDSV 427 G+ VPRAV+VDLEPGTMD+V+SG FG +FRPDN ++GQS AGN WAKGHYTEGAEL D+V Sbjct: 57 GKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAV 116 Query: 428 LDVVRKEAEGCDCLQGFQLTHSLGGGT 508 LDVVR+EAE CD LQGFQLTHSLGGGT Sbjct: 117 LDVVRREAEACDALQGFQLTHSLGGGT 143 Score = 127 bits (306), Expect = 2e-30 Identities = 66/115 (57%), Positives = 77/115 (66%) Frame = +1 Query: 472 GFSANSFSGRRY*SGMGNLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQ 651 GF G SGMG LL+SKIREEYPDR+M TFSV P+PK SDTVVEPYNATLS+HQ Sbjct: 132 GFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQ 191 Query: 652 LVENTDETYCIDNEALYDICFRT*S*QTPTVWGLEPFVQRQCLV*PRAFEFPGQL 816 LVEN+DET+CIDNEAL I T ++P+ L V +F FPG+L Sbjct: 192 LVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGEL 246 Score = 99.5 bits (237), Expect = 5e-22 Identities = 41/58 (70%), Positives = 49/58 (84%) Frame = +3 Query: 81 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLERINVYYNEASGGK 254 MREIVHIQAGQCGNQ+GA FW I+DEHG+D+ G Y G S+ Q ER+NVY+NEA+GGK Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGK 58 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 109 bits (262), Expect = 5e-25 Identities = 45/88 (51%), Positives = 65/88 (73%) Frame = +2 Query: 245 RGQDVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSEAGNNWAKGHYTEGAELVDS 424 +G+ VPR++ VDLEP +D VR+GP+ +F P+ V G+ +A NN+A+GHYT G E++DS Sbjct: 62 QGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDS 121 Query: 425 VLDVVRKEAEGCDCLQGFQLTHSLGGGT 508 VL+ +R+ A+ C LQGF + HS GGGT Sbjct: 122 VLERIRRMADNCSGLQGFLVFHSFGGGT 149 Score = 81.4 bits (192), Expect = 1e-16 Identities = 40/102 (39%), Positives = 59/102 (57%) Frame = +1 Query: 511 SGMGNLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDN 690 SG+G LL+ ++ EY + FSV P+P+VS +VVEPYN+ L+ H ++N+D T+ +DN Sbjct: 151 SGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDN 210 Query: 691 EALYDICFRT*S*QTPTVWGLEPFVQRQCLV*PRAFEFPGQL 816 EA YDIC R + PT L + + + F G L Sbjct: 211 EACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSL 252 Score = 41.9 bits (94), Expect = 1e-04 Identities = 21/56 (37%), Positives = 31/56 (55%) Frame = +3 Query: 81 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLERINVYYNEASG 248 MRE++ + GQ G QIG WE+ EHGI G + +S ++ + N Y N+ G Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENS--EVHKNNSYLNDGFG 54 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 108 bits (260), Expect = 8e-25 Identities = 46/93 (49%), Positives = 65/93 (69%) Frame = +2 Query: 245 RGQDVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSEAGNNWAKGHYTEGAELVDS 424 +G+ VPR++ VDLEP +D VR+GP+ +F P+ + G+ +A NN+A+GHYT G ELVD Sbjct: 58 QGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDE 117 Query: 425 VLDVVRKEAEGCDCLQGFQLTHSLGGGTDLVWG 523 V D +R+ A+ C LQGF + HS GGGT +G Sbjct: 118 VTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFG 150 Score = 75.8 bits (178), Expect = 7e-15 Identities = 39/108 (36%), Positives = 58/108 (53%) Frame = +1 Query: 511 SGMGNLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDN 690 SG G LL+ ++ EY + FSV P+P+VS +VVEPYN+ L+ H ++ D T+ +DN Sbjct: 147 SGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDN 206 Query: 691 EALYDICFRT*S*QTPTVWGLEPFVQRQCLV*PRAFEFPGQLKRRFAE 834 E+ YDIC R + P+ L + + + F G L AE Sbjct: 207 ESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAE 254 Score = 42.3 bits (95), Expect = 8e-05 Identities = 22/60 (36%), Positives = 31/60 (51%), Gaps = 2/60 (3%) Frame = +3 Query: 81 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLE--RINVYYNEASGGK 254 MREI+ I GQ G QIG WE+ EHGI G + ++ Q + +++E GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 84.6 bits (200), Expect = 2e-17 Identities = 35/93 (37%), Positives = 61/93 (65%), Gaps = 2/93 (2%) Frame = +2 Query: 257 VPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSE--AGNNWAKGHYTEGAELVDSVL 430 +PRA+++DLEP ++++ S +G ++ P+N + ++ AGNNWA G Y+ + + ++ Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119 Query: 431 DVVRKEAEGCDCLQGFQLTHSLGGGTDLVWGIF 529 D++ +EA+G D L+GF L HS+ GGT G F Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSF 152 Score = 65.3 bits (152), Expect = 1e-11 Identities = 35/103 (33%), Positives = 52/103 (50%), Gaps = 1/103 (0%) Frame = +1 Query: 511 SGMGNLLISKIREEYPDRIMNTFSVVP-SPKVSDTVVEPYNATLSVHQLVENTDETYCID 687 SG+G+ L+ ++ + YP +I+ T+SV P S VSD VV+PYN+ L++ +L N D +D Sbjct: 147 SGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLD 206 Query: 688 NEALYDICFRT*S*QTPTVWGLEPFVQRQCLV*PRAFEFPGQL 816 N AL I Q PT V +PG + Sbjct: 207 NAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYM 249 Score = 53.6 bits (123), Expect = 3e-08 Identities = 22/53 (41%), Positives = 36/53 (67%) Frame = +3 Query: 84 REIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLERINVYYNEA 242 REI+ +QAGQCGNQIG++FW+ + EHGI G + ++R +V++ ++ Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQS 55 >SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 29.1 bits (62), Expect = 0.82 Identities = 14/32 (43%), Positives = 18/32 (56%) Frame = -2 Query: 372 PASDCPKTKLSGRKICPNGPERTESMVPGSRS 277 P++ PK L R I PNGPE + + GS S Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51 >SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase complex subunit Pst1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1522 Score = 29.1 bits (62), Expect = 0.82 Identities = 28/85 (32%), Positives = 34/85 (40%), Gaps = 4/85 (4%) Frame = -3 Query: 506 YLLPENELAENPADNRSLLLLF*LHPVPNQPVQPPLCNVLSPSYSPPQ---TVRRRSCPV 336 YL P +A +P N L H P P PL N +SP Y P R + V Sbjct: 30 YLTPSGTMAYHPG-NAPLFTQAPPHTNPQGPPPFPLFNSISPVYDPATGRLLYRNVNTQV 88 Query: 335 GRFA-QTARNGRSPWFQAQGPPSQL 264 A NG + + GPPSQL Sbjct: 89 SHTAIPNPANGYAAVY--GGPPSQL 111 >SPCC2H8.02 |||inorganic phosphate transporter|Schizosaccharomyces pombe|chr 3|||Manual Length = 583 Score = 27.9 bits (59), Expect = 1.9 Identities = 15/47 (31%), Positives = 25/47 (53%), Gaps = 1/47 (2%) Frame = -1 Query: 379 VIPRLRLSEDEVVRSEDLPKRPGT-DGVHGSRLKVHHHSSRDVLPPE 242 +IPRLR+ E E ++ K PG D S++++H ++ L E Sbjct: 239 LIPRLRMQESEQFKNSKNMKSPGEGDLDSASQIELHDFKKKESLTAE 285 >SPBC2A9.03 |||conserved protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 426 Score = 26.2 bits (55), Expect = 5.8 Identities = 14/41 (34%), Positives = 21/41 (51%) Frame = -1 Query: 784 TPDIVAEQMVQVPIRLGFVSFKS*SRYRKAPHCLYNTSHQC 662 +P+ ++ VQV F F+ R+ K P+CL N H C Sbjct: 101 SPEELSLDTVQVAAEDSFYEFQ---RFLKNPNCLRNHHHLC 138 >SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 subunit Alp6|Schizosaccharomyces pombe|chr 2|||Manual Length = 821 Score = 26.2 bits (55), Expect = 5.8 Identities = 10/35 (28%), Positives = 18/35 (51%) Frame = -3 Query: 149 YFPELCSDLIPALTRLDVDDFPHDCTKNLEPPESN 45 +F E+ + P +LD+DD + + P E+N Sbjct: 36 FFQEIIHSISPDTFQLDIDDILYKIYSKIPPEENN 70 >SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr 3|||Manual Length = 378 Score = 26.2 bits (55), Expect = 5.8 Identities = 23/67 (34%), Positives = 30/67 (44%) Frame = +2 Query: 326 QIFRPDNFVFGQSEAGNNWAKGHYTEGAELVDSVLDVVRKEAEGCDCLQGFQLTHSLGGG 505 +++ D FG S G +G T G LV LD A + L F HS+GGG Sbjct: 46 EVYTFDQRGFGHSRKGGPKKQG-CTGGWSLVFPDLDYQILRASDTE-LPLFLWGHSMGGG 103 Query: 506 TDLVWGI 526 L +GI Sbjct: 104 LALRYGI 110 >SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 25.8 bits (54), Expect = 7.7 Identities = 17/53 (32%), Positives = 26/53 (49%) Frame = +1 Query: 46 FDSGGSKFLVQS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPT 204 F S S S +STS RV++ ++ SSG + T+ + V +AT T Sbjct: 165 FVSPSSSSSSSSSAATSTSTRVSSSAKASTSSGAIAYTTKCVVVPVTASATAT 217 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 25.8 bits (54), Expect = 7.7 Identities = 12/30 (40%), Positives = 18/30 (60%) Frame = +2 Query: 383 AKGHYTEGAELVDSVLDVVRKEAEGCDCLQ 472 A+GH G ELV + D +RK++E L+ Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212 >SPBC1861.09 |ppk22||serine/threonine protein kinase Ppk22 |Schizosaccharomyces pombe|chr 2|||Manual Length = 526 Score = 25.8 bits (54), Expect = 7.7 Identities = 10/36 (27%), Positives = 19/36 (52%) Frame = +2 Query: 296 MDSVRSGPFGQIFRPDNFVFGQSEAGNNWAKGHYTE 403 M ++R + P N++FG++E G G+ T+ Sbjct: 109 MSNIRKAQVKILKNPGNYIFGRTEYGKRTYSGNSTK 144 >SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 25.8 bits (54), Expect = 7.7 Identities = 17/53 (32%), Positives = 26/53 (49%) Frame = +1 Query: 46 FDSGGSKFLVQS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPT 204 F S S S +STS RV++ ++ SSG + T+ + V +AT T Sbjct: 165 FVSPSSSSSSSSSAATSTSTRVSSSAKASTSSGAIAYTTKCVVVPVTASATAT 217 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,806,804 Number of Sequences: 5004 Number of extensions: 84203 Number of successful extensions: 290 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 269 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 286 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 416455520 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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