BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesV0428.Seq (878 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 188 7e-49 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 101 1e-22 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 100 5e-22 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 98 2e-21 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 63 4e-11 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 63 4e-11 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 52 1e-07 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 36 0.010 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.12 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 30 0.50 SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 29 0.87 SPBC609.02 |ptn1||phosphatidylinositol-3,4,5-trisphosphate3-phos... 27 2.7 SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 27 2.7 SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 3.5 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 4.7 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 26 6.1 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 6.1 SPAC17H9.04c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 26 6.1 SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pomb... 26 6.1 SPCC576.09 |rps20||40S ribosomal protein S20|Schizosaccharomyces... 26 8.1 SPBC18H10.21c ||SPBC9B6.01c|dubious|Schizosaccharomyces pombe|ch... 26 8.1 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 188 bits (459), Expect = 7e-49 Identities = 95/129 (73%), Positives = 98/129 (75%) Frame = +3 Query: 255 AQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANRE 434 AQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NRE Sbjct: 58 AQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNRE 117 Query: 435 KMTQIMFETFNTPAMYVAIQAVLS*RVRFVPPVSCWTPATVSSHTVPIYEGYALPPRHPA 614 KMTQI+FETFN PA YVAIQAVLS +HTVPIYEGYALP Sbjct: 118 KMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMR 177 Query: 615 SGL*PGRDL 641 L GRDL Sbjct: 178 LDL-AGRDL 185 Score = 119 bits (287), Expect = 5e-28 Identities = 52/60 (86%), Positives = 58/60 (96%) Frame = +1 Query: 88 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEHRAK 267 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDE ++K Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 101 bits (242), Expect = 1e-22 Identities = 46/114 (40%), Positives = 73/114 (64%) Frame = +3 Query: 255 AQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANRE 434 A++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P+NP ANRE Sbjct: 58 AEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANRE 117 Query: 435 KMTQIMFETFNTPAMYVAIQAVLS*RVRFVPPVSCWTPATVSSHTVPIYEGYAL 596 KM + MFE + +YVAIQAVLS + + +H VP+YE L Sbjct: 118 KMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVL 171 Score = 52.4 bits (120), Expect = 8e-08 Identities = 29/59 (49%), Positives = 36/59 (61%), Gaps = 2/59 (3%) Frame = +1 Query: 94 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEHRA 264 E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDE A Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEA 60 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 99.5 bits (237), Expect = 5e-22 Identities = 47/116 (40%), Positives = 72/116 (62%), Gaps = 1/116 (0%) Frame = +3 Query: 255 AQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANR 431 AQ+ RG+L ++ PIE GI+ NW DME+IW + + ++ L PEEHP+LLTE PL N+ Sbjct: 62 AQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNK 121 Query: 432 EKMTQIMFETFNTPAMYVAIQAVLS*RVRFVPPVSCWTPATVSSHTVPIYEGYALP 599 EK+ + +ET N PA+ ++Q VL+ +H+VPIY+G+++P Sbjct: 122 EKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIP 177 Score = 65.7 bits (153), Expect = 8e-12 Identities = 28/50 (56%), Positives = 36/50 (72%) Frame = +1 Query: 106 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 255 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG E Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSE 61 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 97.9 bits (233), Expect = 2e-21 Identities = 48/114 (42%), Positives = 64/114 (56%), Gaps = 8/114 (7%) Frame = +3 Query: 279 TLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFE 458 +L YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFE 148 Query: 459 TFNTPAMYVAIQAVLS*RVRFVPPV---SCWTPATVSS-----HTVPIYEGYAL 596 +FN +Y+A+QAVL+ + T V S H +P+ EGY + Sbjct: 149 SFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVI 202 Score = 42.7 bits (96), Expect = 7e-05 Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%) Frame = +1 Query: 106 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGDE 255 +++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ + Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 63.3 bits (147), Expect = 4e-11 Identities = 32/101 (31%), Positives = 52/101 (51%), Gaps = 2/101 (1%) Frame = +3 Query: 291 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNT 470 P E +++NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144 Query: 471 PAMYVAIQAVLS*RVRFVPPVS--CWTPATVSSHTVPIYEG 587 P++ I + S P S +SH +P+ G Sbjct: 145 PSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVLNG 185 Score = 35.9 bits (79), Expect = 0.008 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +1 Query: 106 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 201 LV+DNGS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 63.3 bits (147), Expect = 4e-11 Identities = 29/73 (39%), Positives = 41/73 (56%) Frame = +3 Query: 276 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 455 + +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122 Query: 456 ETFNTPAMYVAIQ 494 E+ PA Y+A Q Sbjct: 123 ESLRCPATYLAKQ 135 Score = 40.3 bits (90), Expect = 4e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +1 Query: 91 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 192 +EV+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 51.6 bits (118), Expect = 1e-07 Identities = 21/67 (31%), Positives = 39/67 (58%) Frame = +3 Query: 300 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAM 479 +GI+ +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133 Query: 480 YVAIQAV 500 Y+ QAV Sbjct: 134 YLTKQAV 140 Score = 37.1 bits (82), Expect = 0.003 Identities = 19/55 (34%), Positives = 32/55 (58%) Frame = +1 Query: 91 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 255 EE+ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDE 56 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 35.5 bits (78), Expect = 0.010 Identities = 21/75 (28%), Positives = 34/75 (45%), Gaps = 4/75 (5%) Frame = +3 Query: 291 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 458 PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 459 TFNTPAMYVAIQAVL 503 P +A + ++ Sbjct: 172 ECQVPGFTIAYEPLM 186 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 31.9 bits (69), Expect = 0.12 Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +3 Query: 351 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAM---YVAIQ 494 +Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 29.9 bits (64), Expect = 0.50 Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 1/55 (1%) Frame = -1 Query: 248 PT*ESFCPI-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 87 PT S P+ PT+ P P N S P PL+TTN TS+S Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455 >SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 277 Score = 29.1 bits (62), Expect = 0.87 Identities = 18/66 (27%), Positives = 30/66 (45%) Frame = -1 Query: 323 IPVSDDSVFDGVFEGQDTSFALCSSPT*ESFCPIPTITP*WRGLPTIEGNTARGASSPAK 144 +P + +V +TSFAL ++ S PT + P +E + +S+PA Sbjct: 63 LPTQEAAVETNASASHETSFALPTTSPAASLSISPTKSAAVSSEPNVEADVKSLSSTPAA 122 Query: 143 PALHIP 126 P L+ P Sbjct: 123 PQLNSP 128 >SPBC609.02 |ptn1||phosphatidylinositol-3,4, 5-trisphosphate3-phosphatase|Schizosaccharomyces pombe|chr 2|||Manual Length = 348 Score = 27.5 bits (58), Expect = 2.7 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = +3 Query: 516 RFVPPVSCWTPATVSSHTVPIYEG 587 R + PV+ W + +SSH V I EG Sbjct: 229 RNINPVALWKSSDISSHNVSIKEG 252 >SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 27.5 bits (58), Expect = 2.7 Identities = 13/27 (48%), Positives = 15/27 (55%) Frame = -2 Query: 250 LLHKSPSVPYRPSRPDGGAFPRSRGTR 170 L+ K+PS PY SRP A S TR Sbjct: 309 LISKAPSYPYSSSRPSASASLASSPTR 335 >SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces pombe|chr 2|||Manual Length = 1485 Score = 27.1 bits (57), Expect = 3.5 Identities = 18/60 (30%), Positives = 29/60 (48%) Frame = +1 Query: 634 ATFTDYFMKILHRARVIRSTTTAERGNSFRGHSKGKAVLTSALDFRARRLANRLHSKQLP 813 A F+DY + V+ +TTT + ++ SK +A S D R L + S+Q+P Sbjct: 5 ADFSDYEDEASGDENVLPNTTTKRKAST--TSSKSRAKKASTPDLRQTSLTSMTASEQIP 62 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.6 bits (56), Expect = 4.7 Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 2/41 (4%) Frame = -1 Query: 251 SPT*ESFC--PIPTITP*WRGLPTIEGNTARGASSPAKPAL 135 S T +SF P+PT P LPT NT + P+ PAL Sbjct: 64 SNTPKSFAAPPVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 26.2 bits (55), Expect = 6.1 Identities = 13/35 (37%), Positives = 20/35 (57%) Frame = +1 Query: 151 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 255 G+ PRA F ++ P H G+++ M KD G+E Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEE 534 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 6.1 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +3 Query: 393 LLTEAPLNPKANREKMTQIMFETFN 467 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPAC17H9.04c |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 604 Score = 26.2 bits (55), Expect = 6.1 Identities = 15/37 (40%), Positives = 20/37 (54%) Frame = +2 Query: 716 RFVDIQRGKLC*RRLLTFEQGDWPTGCIPSSFLRRKS 826 RF D R K R +T +QGDW C ++F RR + Sbjct: 542 RFDDHGRFKEVSRPSVTTDQGDWLCECGFTNFRRRSN 578 >SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1379 Score = 26.2 bits (55), Expect = 6.1 Identities = 23/86 (26%), Positives = 32/86 (37%) Frame = +1 Query: 463 STRPPCTSPSKPCSRDASGSYHRYRAGLRRRCLPTPCPSTRDTHFPHAILRLDFSPVATF 642 S R S + + SG Y R R+R L P P T H P + R A Sbjct: 115 SRRGGAASNAAAIAAKKSGYYER--TSTRKRSLTVPTPRTSFPHHPRS-RRFSLKNAAIA 171 Query: 643 TDYFMKILHRARVIRSTTTAERGNSF 720 T Y H+ + + S + + G F Sbjct: 172 TSY-----HKFKPLSSNSLSSSGMHF 192 >SPCC576.09 |rps20||40S ribosomal protein S20|Schizosaccharomyces pombe|chr 3|||Manual Length = 118 Score = 25.8 bits (54), Expect = 8.1 Identities = 17/48 (35%), Positives = 27/48 (56%), Gaps = 2/48 (4%) Frame = -3 Query: 675 RSVKNLHEVVCEGRDRAKVQTQDGVGEVRIPRRWAR--CGKTPSPESS 538 R+V+NL +V + +RAK + G VR+P + + KTP+ E S Sbjct: 27 RNVRNLEKVCSDLVNRAKDKQLRVKGPVRLPTKILKITTRKTPNGEGS 74 >SPBC18H10.21c ||SPBC9B6.01c|dubious|Schizosaccharomyces pombe|chr 2|||Manual Length = 157 Score = 25.8 bits (54), Expect = 8.1 Identities = 9/27 (33%), Positives = 14/27 (51%) Frame = +1 Query: 532 YRAGLRRRCLPTPCPSTRDTHFPHAIL 612 Y + +R P+ P R TH PH ++ Sbjct: 54 YPESVSKRARPSQKPPLRSTHLPHLLI 80 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,925,696 Number of Sequences: 5004 Number of extensions: 89430 Number of successful extensions: 281 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 245 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 271 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 440481800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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