Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesV0425.Seq
(748 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g44635.1 68418.m05469 minichromosome maintenance family prote... 89 2e-18
At2g16440.1 68415.m01883 DNA replication licensing factor, putat... 64 1e-10
At5g46280.1 68418.m05697 DNA replication licensing factor, putat... 62 5e-10
At4g02060.1 68417.m00276 prolifera protein (PRL) / DNA replicati... 60 2e-09
At2g07690.1 68415.m00993 minichromosome maintenance family prote... 58 5e-09
At3g09660.1 68416.m01145 minichromosome maintenance family prote... 58 8e-09
At1g44900.1 68414.m05144 DNA replication licensing factor, putat... 49 3e-06
At2g14050.1 68415.m01563 minichromosome maintenance family prote... 45 5e-05
At1g19580.1 68414.m02439 bacterial transferase hexapeptide repea... 29 4.3
At1g74670.1 68414.m08647 gibberellin-responsive protein, putativ... 28 7.6
At3g11385.1 68416.m01386 DC1 domain-containing protein contains ... 27 10.0
>At5g44635.1 68418.m05469 minichromosome maintenance family protein
/ MCM family protein similar to SP|P97311 DNA
replication licensing factor MCM6 {Mus musculus};
contains Pfam profile PF00493: MCM2/3/5 family
Length = 831
Score = 89.4 bits (212), Expect = 2e-18
Identities = 39/69 (56%), Positives = 53/69 (76%)
Frame = +2
Query: 2 KSVFVDFQKIRIQETQAELPRGCIPRSLEVILRAEAVESVQAGDRYDFTGTLIVVPDVGA 181
+S F D+Q++R+QET E+P G +PRSL+VILR E VE +AGD FTGT++V+PD+ A
Sbjct: 194 ESKFADWQRVRMQETSKEIPAGSLPRSLDVILRHEIVEQARAGDTVIFTGTVVVIPDISA 253
Query: 182 LSMPGSRAE 208
L+ PG RAE
Sbjct: 254 LAAPGERAE 262
Score = 74.5 bits (175), Expect = 7e-14
Identities = 41/72 (56%), Positives = 47/72 (65%)
Frame = +3
Query: 513 VLLMLFGGVAKTTIEGTTLRGDINVCIVGDPSTAKSQLLKQVSEITPRGRVH*AERLPLL 692
VLLML GGV KTT EG LRGDINVCIVGDPS AKSQ LK + I PR V+ + +
Sbjct: 367 VLLMLLGGVHKTTHEGINLRGDINVCIVGDPSCAKSQFLKYTAGIVPRS-VYTSGKSSSA 425
Query: 693 LVFTAAVVRDEE 728
TA V ++ E
Sbjct: 426 AGLTATVAKEPE 437
Score = 64.1 bits (149), Expect = 9e-11
Identities = 31/86 (36%), Positives = 53/86 (61%)
Frame = +1
Query: 256 KGLKALGVRELHYKTAFLACSVQAVSRRFGTAELPTHDLTTEDMRKQMTDKEWDKVYEMS 435
+GLKALGVR+L Y+ AF+A SVQ T + + ED ++Q T +E D++ +M
Sbjct: 281 QGLKALGVRDLSYRLAFIANSVQIADGSRNTDMRNRQNDSNEDDQQQFTAEELDEIQQMR 340
Query: 436 RDRNLYNNLITSLFPSIHGNNEVKQA 513
+ +N L+ S+ P++ G+ ++K+A
Sbjct: 341 NTPDYFNKLVGSMAPTVFGHQDIKRA 366
>At2g16440.1 68415.m01883 DNA replication licensing factor, putative
similar to SP|P49717 DNA replication licensing factor
MCM4 (CDC21 homolog) {Mus musculus}, SP|P29458 Cdc21
protein {Schizosaccharomyces pombe}; contains Pfam
profile PF00493: MCM2/3/5 family
Length = 847
Score = 63.7 bits (148), Expect = 1e-10
Identities = 32/73 (43%), Positives = 46/73 (63%)
Frame = +3
Query: 510 GVLLMLFGGVAKTTIEGTTLRGDINVCIVGDPSTAKSQLLKQVSEITPRGRVH*AERLPL 689
G+L LFGG A G RGDIN+ +VGDP T+KSQLL+ + +++PRG ++ + R
Sbjct: 456 GLLCQLFGGNALNLASGANFRGDINILLVGDPGTSKSQLLQYIHKLSPRG-IYTSGRGSS 514
Query: 690 LLVFTAAVVRDEE 728
+ TA V +D E
Sbjct: 515 AVGLTAYVAKDPE 527
Score = 42.3 bits (95), Expect = 3e-04
Identities = 18/47 (38%), Positives = 30/47 (63%)
Frame = +2
Query: 11 FVDFQKIRIQETQAELPRGCIPRSLEVILRAEAVESVQAGDRYDFTG 151
F D Q +R+QET E+P G P ++ ++L + V++ + GDR + TG
Sbjct: 314 FADKQIVRLQETPDEIPEGGTPHTVSLLLHDKLVDNGKPGDRIEVTG 360
>At5g46280.1 68418.m05697 DNA replication licensing factor, putative
similar to SP|Q43704 DNA replication licensing factor
MCM3 homolog (Replication origin activator) (ROA
protein) {Zea mays}; contains Pfam profile PF00493:
MCM2/3/5 family
Length = 776
Score = 61.7 bits (143), Expect = 5e-10
Identities = 36/82 (43%), Positives = 48/82 (58%)
Frame = +3
Query: 483 HSWK**GEAGVLLMLFGGVAKTTIEGTTLRGDINVCIVGDPSTAKSQLLKQVSEITPRGR 662
H+W + V+L++ GGV K GT LRGDIN+ +VGDPS AKSQLL+ + I P
Sbjct: 300 HAWI---KKAVVLLMLGGVEKNLKNGTHLRGDINMMMVGDPSVAKSQLLRAIMNIAPLA- 355
Query: 663 VH*AERLPLLLVFTAAVVRDEE 728
+ R + TAAV D+E
Sbjct: 356 ISTTGRGSSGVGLTAAVTSDQE 377
Score = 36.7 bits (81), Expect = 0.016
Identities = 20/62 (32%), Positives = 30/62 (48%)
Frame = +2
Query: 11 FVDFQKIRIQETQAELPRGCIPRSLEVILRAEAVESVQAGDRYDFTGTLIVVPDVGALSM 190
+ D Q + IQE G +PRS++VI + V+S + GDR G +P S+
Sbjct: 184 YKDHQTLSIQEVPENAAPGQLPRSVDVIAEDDLVDSCKPGDRVSVFGIYKALPGKSKGSV 243
Query: 191 PG 196
G
Sbjct: 244 NG 245
>At4g02060.1 68417.m00276 prolifera protein (PRL) / DNA replication
licensing factor Mcm7 (MCM7) identical to DNA
replication licensing factor Mcm7 SP|P43299 PROLIFERA
protein {Arabidopsis thaliana}; contains Pfam profile
PF00493: MCM2/3/5 family
Length = 716
Score = 59.7 bits (138), Expect = 2e-09
Identities = 29/71 (40%), Positives = 46/71 (64%)
Frame = +3
Query: 513 VLLMLFGGVAKTTIEGTTLRGDINVCIVGDPSTAKSQLLKQVSEITPRGRVH*AERLPLL 692
+LL+L G + +G +RGD+++C++GDP AKSQLLK + + PRG V+ +
Sbjct: 348 LLLLLVGAPHRQLKDGMKIRGDVHICLMGDPGVAKSQLLKHIINVAPRG-VYTTGKGSSG 406
Query: 693 LVFTAAVVRDE 725
+ TAAV+RD+
Sbjct: 407 VGLTAAVMRDQ 417
Score = 50.4 bits (115), Expect = 1e-06
Identities = 23/58 (39%), Positives = 34/58 (58%)
Frame = +2
Query: 5 SVFVDFQKIRIQETQAELPRGCIPRSLEVILRAEAVESVQAGDRYDFTGTLIVVPDVG 178
S F+ FQ+ ++QE +P+G IPRS+ V LR E V GD +F+G + +P G
Sbjct: 221 SKFLKFQEAKMQELAEHVPKGHIPRSMTVHLRGELTRKVSPGDVVEFSGIFLPIPYTG 278
Score = 37.1 bits (82), Expect = 0.012
Identities = 25/88 (28%), Positives = 44/88 (50%)
Frame = +1
Query: 298 TAFLACSVQAVSRRFGTAELPTHDLTTEDMRKQMTDKEWDKVYEMSRDRNLYNNLITSLF 477
T F A V+ + A TH + + D+E +++ ++ D ++YN L SL
Sbjct: 277 TGFKALRAGLVADTYLEATSVTHFKKKYEEYEFQKDEE-EQIARLAEDGDIYNKLSRSLA 335
Query: 478 PSIHGNNEVKQASY*CCLEGSPKLLLKE 561
P I+G+ ++K+A L G+P LK+
Sbjct: 336 PEIYGHEDIKKALL-LLLVGAPHRQLKD 362
>At2g07690.1 68415.m00993 minichromosome maintenance family protein
/ MCM family protein similar to SP|P55862 DNA
replication licensing factor MCM5 (CDC46 homolog)
{Xenopus laevis}; contains Pfam profile PF00493:
MCM2/3/5 family
Length = 727
Score = 58.4 bits (135), Expect = 5e-09
Identities = 32/67 (47%), Positives = 43/67 (64%)
Frame = +3
Query: 522 MLFGGVAKTTIEGTTLRGDINVCIVGDPSTAKSQLLKQVSEITPRGRVH*AERLPLLLVF 701
+LFGG K+ +G LRGDINV ++GDPSTAKSQ LK V + P V+ + +
Sbjct: 350 LLFGGSRKSLPDGVKLRGDINVLLLGDPSTAKSQFLKFVEKTAPIA-VYTSGKGSSAAGL 408
Query: 702 TAAVVRD 722
TA+V+RD
Sbjct: 409 TASVIRD 415
Score = 33.1 bits (72), Expect = 0.20
Identities = 14/50 (28%), Positives = 29/50 (58%)
Frame = +2
Query: 2 KSVFVDFQKIRIQETQAELPRGCIPRSLEVILRAEAVESVQAGDRYDFTG 151
+S +VD Q +++QE ++P G +PR++ + + V+++ G R G
Sbjct: 215 RSQYVDQQTLKLQENPEDVPTGELPRNMLLSVDRHLVQTIVPGTRLTVMG 264
Score = 32.3 bits (70), Expect = 0.35
Identities = 16/51 (31%), Positives = 29/51 (56%)
Frame = +1
Query: 400 TDKEWDKVYEMSRDRNLYNNLITSLFPSIHGNNEVKQASY*CCLEGSPKLL 552
T E ++ + + +++Y N+ T + PSI G+ +VK+A+ GS K L
Sbjct: 309 TPDEEEEFKKFADSQDVYKNICTKIAPSIFGHEDVKRAAACLLFGGSRKSL 359
>At3g09660.1 68416.m01145 minichromosome maintenance family protein
/ MCM family protein similar to SP|P49717 DNA
replication licensing factor MCM4 (CDC21 homolog) {Mus
musculus}; contains Pfam profile PF00493: MCM2/3/5
family
Length = 777
Score = 57.6 bits (133), Expect = 8e-09
Identities = 33/75 (44%), Positives = 46/75 (61%), Gaps = 2/75 (2%)
Frame = +3
Query: 504 EAGVLLMLFGGVAKTTIEGTTL--RGDINVCIVGDPSTAKSQLLKQVSEITPRGRVH*AE 677
+AG+ L LFGGV K +++ + RGDI+V IVGDP KSQLL+ + I+PRG ++
Sbjct: 370 KAGITLSLFGGVRKHSMDRNKVPVRGDIHVIIVGDPGLGKSQLLQAAAAISPRG-IYVCG 428
Query: 678 RLPLLLVFTAAVVRD 722
T AVV+D
Sbjct: 429 NATTRAGLTVAVVKD 443
Score = 35.9 bits (79), Expect = 0.029
Identities = 20/53 (37%), Positives = 30/53 (56%), Gaps = 2/53 (3%)
Frame = +2
Query: 14 VDFQKIRIQETQ--AELPRGCIPRSLEVILRAEAVESVQAGDRYDFTGTLIVV 166
+DFQKIR+QE Q + G +PR++E L + V+ GD TG + V+
Sbjct: 222 IDFQKIRVQELQKPEDHEEGRVPRTVECELMEDLVDICIPGDVVTVTGIIGVI 274
>At1g44900.1 68414.m05144 DNA replication licensing factor, putative
similar to DNA replication licensing factor MCM2 from
{Xenopus laevis} SP|P55861, SP|P49736 {Homo sapiens};
contains Pfam profile PF00493: MCM2/3/5 family
Length = 928
Score = 49.2 bits (112), Expect = 3e-06
Identities = 31/73 (42%), Positives = 41/73 (56%)
Frame = +3
Query: 504 EAGVLLMLFGGVAKTTIEGTTLRGDINVCIVGDPSTAKSQLLKQVSEITPRGRVH*AERL 683
+ + L +FGG K LRGDINV ++GDP TAKSQ LK V E T + V+ +
Sbjct: 510 KTALALAMFGGQEKNIKGKHRLRGDINVLLLGDPGTAKSQFLKYV-EKTGQRAVYTTGKG 568
Query: 684 PLLLVFTAAVVRD 722
+ TAAV +D
Sbjct: 569 ASAVGLTAAVHKD 581
Score = 40.7 bits (91), Expect = 0.001
Identities = 16/50 (32%), Positives = 32/50 (64%)
Frame = +2
Query: 2 KSVFVDFQKIRIQETQAELPRGCIPRSLEVILRAEAVESVQAGDRYDFTG 151
++++ ++QK+ IQE+ +P G +PR EVIL + ++ + G+ + TG
Sbjct: 385 QTIYRNYQKLTIQESPGTVPAGRLPRHKEVILLNDLIDCARPGEEIEVTG 434
Score = 33.5 bits (73), Expect = 0.15
Identities = 12/40 (30%), Positives = 27/40 (67%)
Frame = +1
Query: 394 QMTDKEWDKVYEMSRDRNLYNNLITSLFPSIHGNNEVKQA 513
++T ++ ++ E+S+D + +I S+ PSI+G+ ++K A
Sbjct: 473 KLTQEDKTQIEELSKDPRIVERIIKSIAPSIYGHEDIKTA 512
>At2g14050.1 68415.m01563 minichromosome maintenance family protein
/ MCM family protein low similarity to SP|P49736 DNA
replication licensing factor MCM2 {Homo sapiens};
contains Pfam profile PF00493: MCM2/3/5 family
Length = 653
Score = 45.2 bits (102), Expect = 5e-05
Identities = 20/48 (41%), Positives = 30/48 (62%)
Frame = +3
Query: 513 VLLMLFGGVAKTTIEGTTLRGDINVCIVGDPSTAKSQLLKQVSEITPR 656
V L L GGV GT +RG+ ++ ++GDP T KSQ LK ++++ R
Sbjct: 330 VALTLIGGVQHVDASGTKVRGESHLLLIGDPGTGKSQFLKFAAKLSNR 377
Score = 43.2 bits (97), Expect = 2e-04
Identities = 21/47 (44%), Positives = 32/47 (68%)
Frame = +2
Query: 17 DFQKIRIQETQAELPRGCIPRSLEVILRAEAVESVQAGDRYDFTGTL 157
D+Q+I+IQE L G IPRS+ V+L+ + V++V+AGD +G L
Sbjct: 204 DYQEIKIQENTQVLGVGVIPRSILVVLKDDLVDNVKAGDDVVVSGIL 250
>At1g19580.1 68414.m02439 bacterial transferase hexapeptide
repeat-containing protein contains Pfam profile PF00132:
Bacterial transferase hexapeptide (four repeats)
Length = 275
Score = 28.7 bits (61), Expect = 4.3
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +3
Query: 558 GTTLRGDINVCIVGDPSTAKSQLLKQVSEITPRGRVH 668
G LRGD+N VG + + L V++ G+VH
Sbjct: 82 GCVLRGDVNTVSVGSGTNIQDNSLVHVAKSNLSGKVH 118
>At1g74670.1 68414.m08647 gibberellin-responsive protein, putative
similar to SP|P46690 Gibberellin-regulated protein 4
precursor {Arabidopsis thaliana} GASA4; contains Pfam
profile PF02704: Gibberellin regulated protein
Length = 101
Score = 27.9 bits (59), Expect = 7.6
Identities = 9/22 (40%), Positives = 11/22 (50%)
Frame = -1
Query: 445 CDLETFHKPCPILCQSFVSSCL 380
C +HKPC CQ + CL
Sbjct: 51 CSNTKYHKPCMFFCQKCCAKCL 72
>At3g11385.1 68416.m01386 DC1 domain-containing protein contains
Pfam protein PF03107 DC1 domain
Length = 766
Score = 27.5 bits (58), Expect = 10.0
Identities = 10/26 (38%), Positives = 14/26 (53%)
Frame = +3
Query: 246 WKAKGTQGSWSARTAL*NSIFSMQCT 323
W K QGSWS R ++ N + C+
Sbjct: 297 WCGKSIQGSWSYRCSICNFCLDLSCS 322
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,486,059
Number of Sequences: 28952
Number of extensions: 338695
Number of successful extensions: 910
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 854
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 906
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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