BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesV0424.Seq (698 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 151 9e-38 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 145 6e-36 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 123 3e-29 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 102 4e-23 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 70 3e-13 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 46 5e-06 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 38 0.001 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 30 0.28 SPBC16D10.07c |sir2||Sir2 family histone deacetylase Sir2|Schizo... 30 0.37 SPCP1E11.04c |pal1||membrane associated protein Pal1 |Schizosacc... 27 2.6 SPAC3A12.06c |||sodium/calcium exchanger |Schizosaccharomyces po... 25 7.9 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 151 bits (366), Expect = 9e-38 Identities = 82/155 (52%), Positives = 104/155 (67%), Gaps = 7/155 (4%) Frame = +3 Query: 243 SQEPVALNPNNTVFDAKRLIGRKFDDPKIQQDMKHWPFKVINDCGKPKIQIEFKGETKRF 422 ++ VA+NP+NT+FDAKRLIGRKFDDP++Q DMKHWPFKVI+ GKP +Q+E+KGETK F Sbjct: 53 AKNQVAMNPHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTF 112 Query: 423 APEEISSMVLTKMKXTAEAIWEVQCGMRYHSSGILQRLPASGHQGRRSHSR-------LK 581 PEEISSMVL KM+ TAEA G + + + +PA + +R ++ L Sbjct: 113 TPEEISSMVLMKMRETAEA----YLGGKVTDAVV--TVPAYFNDSQRQATKDAGLIAGLN 166 Query: 582 RARIXNXPTAAALRVRSRQEPQGERNVLIFVLGGG 686 RI N PTAAA+ + QGE NVLIF LGGG Sbjct: 167 VLRIINEPTAAAIAYGLDRSNQGESNVLIFDLGGG 201 Score = 131 bits (317), Expect = 8e-32 Identities = 86/202 (42%), Positives = 102/202 (50%), Gaps = 2/202 (0%) Frame = +1 Query: 97 AIGIDLGTTYSCVGVWQHGNVEIIANXQGNRTTPSYVAFTDTERLIGDAAKNQSP*TLTT 276 +IGIDLGTTYSCVG + + VEIIAN QGNRTTPSYVAFTDTERLIGDAAKNQ Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63 Query: 277 PCSTRRG*SGGNXXXXXXXXXXXXXXXXXXXXAANRKYRSSSKVRRNDLRQKKLAAWC*Q 456 + G + K +++++ Sbjct: 64 TIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSMVLM 123 Query: 457 K*RXRRK--LSGKYSAGCGITVPAYFNDSQRQXTKDAGAIAG*NVLASXTXPQPPRXAYG 630 K R + L GK + +TVPAYFNDSQRQ TKDAG IAG NVL P AYG Sbjct: 124 KMRETAEAYLGGKVTDAV-VTVPAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYG 182 Query: 631 LDKNLKVREMFLSSFWVEGTFD 696 LD++ + L GTFD Sbjct: 183 LDRSNQGESNVLIFDLGGGTFD 204 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 145 bits (351), Expect = 6e-36 Identities = 80/155 (51%), Positives = 102/155 (65%), Gaps = 7/155 (4%) Frame = +3 Query: 243 SQEPVALNPNNTVFDAKRLIGRKFDDPKIQQDMKHWPFKVINDCGKPKIQIEFKGETKRF 422 ++ VA+NP+NT+FDAKRLIGR+F+DP++Q DMKHWPFKVI GKP IQ+EFKGETK F Sbjct: 53 AKNQVAMNPHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTF 112 Query: 423 APEEISSMVLTKMKXTAEAIWEVQCGMRYHSSGILQRLPASGHQGRRSHSR-------LK 581 PEEISSMVL KM+ +AEA G + + + +PA + +R ++ L Sbjct: 113 TPEEISSMVLLKMRESAEAF----LGGKVTDAVV--TVPAYFNDSQRQATKDAGLIAGLN 166 Query: 582 RARIXNXPTAAALRVRSRQEPQGERNVLIFVLGGG 686 RI N PTAAA+ + Q E NVLIF LGGG Sbjct: 167 VLRIINEPTAAAIAYGLDRSNQHETNVLIFDLGGG 201 Score = 96.7 bits (230), Expect = 3e-21 Identities = 45/53 (84%), Positives = 48/53 (90%) Frame = +1 Query: 97 AIGIDLGTTYSCVGVWQHGNVEIIANXQGNRTTPSYVAFTDTERLIGDAAKNQ 255 +IGIDLGTTYSCVG + + VEIIAN QGNRTTPSYVAFTDTERLIGDAAKNQ Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQ 56 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 123 bits (296), Expect = 3e-29 Identities = 69/155 (44%), Positives = 97/155 (62%), Gaps = 7/155 (4%) Frame = +3 Query: 243 SQEPVALNPNNTVFDAKRLIGRKFDDPKIQQDMKHWPFKVINDCGKPKIQIEFKGETKRF 422 ++ A+NP NTVFDAKRLIGR+++DP+ Q+D+KHWPFKVI++ G P I++ + GE K+F Sbjct: 57 AKNQAAMNPRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQF 116 Query: 423 APEEISSMVLTKMKXTAEAIWEVQCGMRYHSSGILQRLPASGHQGRRSHSR-------LK 581 +EIS+MVLTKMK +EA + R + I +PA +R+ ++ L Sbjct: 117 TAQEISAMVLTKMKEISEA----KLNKRVEKAVI--TVPAYFSDSQRAATKDAGAIAGLN 170 Query: 582 RARIXNXPTAAALRVRSRQEPQGERNVLIFVLGGG 686 RI N PTAAA+ + +NVLIF LGGG Sbjct: 171 VLRIINEPTAAAIAYGLDAKSDKPKNVLIFDLGGG 205 Score = 95.9 bits (228), Expect = 5e-21 Identities = 43/54 (79%), Positives = 48/54 (88%) Frame = +1 Query: 97 AIGIDLGTTYSCVGVWQHGNVEIIANXQGNRTTPSYVAFTDTERLIGDAAKNQS 258 AIGIDLGTTYSCV VW+ NVEII N QG RTTPS+VAFT+TERL+GDAAKNQ+ Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQA 61 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 102 bits (245), Expect = 4e-23 Identities = 56/148 (37%), Positives = 89/148 (60%), Gaps = 7/148 (4%) Frame = +3 Query: 264 NPNNTVFDAKRLIGRKFDDPKIQQDMKHWPFKVINDCGKPKIQIEFKGETKRFAPEEISS 443 NP NT+FD KRLIGRKFD+ + +D+K +PF ++ND +P +++ G+ K+F PEEIS+ Sbjct: 93 NPENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISA 152 Query: 444 MVLTKMKXTAEAIWEVQCGMRYHSSGILQRLPASGHQGRRSHSR-------LKRARIXNX 602 M+L+KMK TAEA + + + +PA + +R ++ L RI N Sbjct: 153 MILSKMKQTAEAY------LGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVIRIVNE 206 Query: 603 PTAAALRVRSRQEPQGERNVLIFVLGGG 686 PTAAA+ + E++++++ LGGG Sbjct: 207 PTAAAI-AYGLDKTDTEKHIVVYDLGGG 233 Score = 93.5 bits (222), Expect = 3e-20 Identities = 42/54 (77%), Positives = 48/54 (88%) Frame = +1 Query: 100 IGIDLGTTYSCVGVWQHGNVEIIANXQGNRTTPSYVAFTDTERLIGDAAKNQSP 261 IGIDLGTTYSCV V ++G VEIIAN QGNR TPSYVAFT+ ERL+G+AAKNQ+P Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAP 91 Score = 61.7 bits (143), Expect = 1e-10 Identities = 34/71 (47%), Positives = 40/71 (56%) Frame = +1 Query: 484 GKYSAGCGITVPAYFNDSQRQXTKDAGAIAG*NVLASXTXPQPPRXAYGLDKNLKVREMF 663 GK +TVPAYFND+QRQ TKDAG IAG NV+ P AYGLDK + + Sbjct: 167 GKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKTDTEKHIV 226 Query: 664 LSSFWVEGTFD 696 + GTFD Sbjct: 227 VYDLG-GGTFD 236 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 70.1 bits (164), Expect = 3e-13 Identities = 48/154 (31%), Positives = 82/154 (53%), Gaps = 6/154 (3%) Frame = +3 Query: 243 SQEPVALNPNNTVFDAKRLIGRKFDDPKIQQDMKHWPFKVINDCGKPKIQIEFKGETKRF 422 ++ +NP NT F KRLIGR+F +P++Q+D+K P+K++ + +E +G+T + Sbjct: 102 AKRQAVINPENTFFATKRLIGRRFKEPEVQRDIKEVPYKIV-EHSNGDAWLEARGKT--Y 158 Query: 423 APEEISSMVLTKMKXTAEAIW--EVQCGM----RYHSSGILQRLPASGHQGRRSHSRLKR 584 +P +I +L+KM+ TA +V+ + Y + Q A+G + + L Sbjct: 159 SPSQIGGFILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATKAAG-----AIAGLNV 213 Query: 585 ARIXNXPTAAALRVRSRQEPQGERNVLIFVLGGG 686 R+ N PTAAAL + + + V +F LGGG Sbjct: 214 LRVVNEPTAAALAY--GLDKKNDAIVAVFDLGGG 245 Score = 67.3 bits (157), Expect = 2e-12 Identities = 33/58 (56%), Positives = 42/58 (72%), Gaps = 1/58 (1%) Frame = +1 Query: 88 KMPAIGIDLGTTYSCVGVWQHGNVEIIANXQGNRTTPSYVAFT-DTERLIGDAAKNQS 258 K P IGIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q+ Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQA 106 Score = 61.7 bits (143), Expect = 1e-10 Identities = 32/52 (61%), Positives = 33/52 (63%) Frame = +1 Query: 484 GKYSAGCGITVPAYFNDSQRQXTKDAGAIAG*NVLASXTXPQPPRXAYGLDK 639 GK +TVPAYFNDSQRQ TK AGAIAG NVL P AYGLDK Sbjct: 180 GKDVKNAVVTVPAYFNDSQRQATKAAGAIAGLNVLRVVNEPTAAALAYGLDK 231 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 46.0 bits (104), Expect = 5e-06 Identities = 18/51 (35%), Positives = 34/51 (66%) Frame = +1 Query: 100 IGIDLGTTYSCVGVWQHGNVEIIANXQGNRTTPSYVAFTDTERLIGDAAKN 252 +GID G + + + V ++ +++I N NR+TPS V++ + R +G+AAK+ Sbjct: 8 VGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKS 58 Score = 38.3 bits (85), Expect = 0.001 Identities = 24/75 (32%), Positives = 38/75 (50%), Gaps = 1/75 (1%) Frame = +3 Query: 258 ALNPNNTVFDAKRLIGRKFDDPKIQQ-DMKHWPFKVINDCGKPKIQIEFKGETKRFAPEE 434 A N NTV KRL GR +DDP+I+ + K+ G ++++ E F+ + Sbjct: 61 ASNFRNTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQ 120 Query: 435 ISSMVLTKMKXTAEA 479 + + TK+K AEA Sbjct: 121 LIAAYFTKIKAIAEA 135 Score = 31.1 bits (67), Expect = 0.16 Identities = 16/44 (36%), Positives = 22/44 (50%) Frame = +1 Query: 508 ITVPAYFNDSQRQXTKDAGAIAG*NVLASXTXPQPPRXAYGLDK 639 I++PA+F D QR+ +A IAG N L YG+ K Sbjct: 146 ISIPAWFTDIQRRALLEAANIAGLNPLRLMNDNAAAALTYGITK 189 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 38.3 bits (85), Expect = 0.001 Identities = 40/169 (23%), Positives = 63/169 (37%), Gaps = 6/169 (3%) Frame = +1 Query: 100 IGIDLGTTYSCVGVWQHGNVEIIANXQGNRTTPSYVAFTDTERLIGDAAKNQSP*TLTTP 279 IGI G S + + G +++AN +GNR PS +++ + G A+ Q Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRNADNS 86 Query: 280 CSTRRG*SGGNXXXXXXXXXXXXXXXXXXXXAANRKYRSSSKVRRNDLRQKKLAAWC*QK 459 + R G + K + + +D ++K L A Sbjct: 87 VTNFRDLLGKSHDELTLHHCHYSANPVNVEGQIGFKI-TVQEDEESDPKEKILTAHEASV 145 Query: 460 *RXRR------KLSGKYSAGCGITVPAYFNDSQRQXTKDAGAIAG*NVL 588 RR G GC ++VP YF D+QR+ + A AG VL Sbjct: 146 RHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKALESAANEAGLPVL 194 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 30.3 bits (65), Expect = 0.28 Identities = 24/79 (30%), Positives = 35/79 (44%), Gaps = 1/79 (1%) Frame = +1 Query: 19 LKTSFSTIYLLFIVKKYLQ*LIFKMPAIGIDLGTTYSCVGVWQHG-NVEIIANXQGNRTT 195 +K S TI L F + + F + ID GT ++ + + G +EI+ R Sbjct: 1 MKRSVLTIILFFSCQFWHA---FASSVLAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKE 57 Query: 196 PSYVAFTDTERLIGDAAKN 252 S VAF ER+ G A N Sbjct: 58 QSAVAFKGNERIFGVDASN 76 Score = 25.8 bits (54), Expect = 6.0 Identities = 12/28 (42%), Positives = 17/28 (60%) Frame = +1 Query: 508 ITVPAYFNDSQRQXTKDAGAIAG*NVLA 591 +TVP +FN+ QR +A I +VLA Sbjct: 165 LTVPPHFNELQRSILLEAARILNKHVLA 192 >SPBC16D10.07c |sir2||Sir2 family histone deacetylase Sir2|Schizosaccharomyces pombe|chr 2|||Manual Length = 475 Score = 29.9 bits (64), Expect = 0.37 Identities = 10/37 (27%), Positives = 22/37 (59%) Frame = +3 Query: 309 KFDDPKIQQDMKHWPFKVINDCGKPKIQIEFKGETKR 419 K D ++ +D+++ N+CGKP +++ G+ K+ Sbjct: 280 KVDGSELYEDIRNQRVSYCNECGKPPLKLRRVGQNKK 316 >SPCP1E11.04c |pal1||membrane associated protein Pal1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 425 Score = 27.1 bits (57), Expect = 2.6 Identities = 11/38 (28%), Positives = 23/38 (60%), Gaps = 2/38 (5%) Frame = +3 Query: 231 HRRRSQE--PVALNPNNTVFDAKRLIGRKFDDPKIQQD 338 HR R+ + PVA P +++ ++ +G ++DP + +D Sbjct: 189 HRNRNSKKAPVAAFPKDSIANSIPKVGETYNDPSVPKD 226 >SPAC3A12.06c |||sodium/calcium exchanger |Schizosaccharomyces pombe|chr 1|||Manual Length = 743 Score = 25.4 bits (53), Expect = 7.9 Identities = 21/90 (23%), Positives = 39/90 (43%), Gaps = 2/90 (2%) Frame = -2 Query: 508 YRIP--HCTSQIASAVXFIFVSTMLLISSGANRFVSPLNSICIFGLPQSLITLNGQCFMS 335 ++IP H +A I + M ++ G+ L I + L + +G +S Sbjct: 206 FKIPRRHFLRDVAFLTGTILLVIMFVLHDGSLSIWQSLVMILYYLLYVLFVFFSGSSGVS 265 Query: 334 C*ILGSSNFLPISLFASNTVLLGFKATGSW 245 ++ N+LP+SL + VL F + S+ Sbjct: 266 V-VITDENYLPVSLPVYSPVLNSFDDSDSY 294 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,641,334 Number of Sequences: 5004 Number of extensions: 51439 Number of successful extensions: 171 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 147 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 166 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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