BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesV0420.Seq (547 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g35040.1 68415.m04299 AICARFT/IMPCHase bienzyme family protei... 50 1e-06 At2g45150.3 68415.m05621 phosphatidate cytidylyltransferase fami... 29 2.0 At2g45150.2 68415.m05619 phosphatidate cytidylyltransferase fami... 29 2.0 At2g45150.1 68415.m05620 phosphatidate cytidylyltransferase fami... 29 2.0 At3g48950.1 68416.m05347 glycoside hydrolase family 28 protein /... 28 3.5 At2g32560.1 68415.m03977 F-box family protein contains Pfam PF00... 28 3.5 At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DN... 27 8.2 >At2g35040.1 68415.m04299 AICARFT/IMPCHase bienzyme family protein similar to SP|P12048 Bifunctional purine biosynthesis protein purH [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3) (AICAR transformylase); IMP cyclohydrolase (EC 3.5.4.10) (Inosinicase) (IMP synthetase) (ATIC)] {Bacillus subtilis}; contains Pfam profiles PF01808: AICARFT/IMPCHase bienzyme, PF02142: MGS-like domain Length = 596 Score = 50.0 bits (114), Expect = 1e-06 Identities = 22/35 (62%), Positives = 26/35 (74%) Frame = +3 Query: 132 GLTVQDVSDITRAPEMLGGRVKTLHPAVHAGILAR 236 G++V V +T PEML GRVKTLHP +H GILAR Sbjct: 112 GVSVTKVEKLTHFPEMLDGRVKTLHPNIHGGILAR 146 Score = 40.7 bits (91), Expect = 6e-04 Identities = 35/128 (27%), Positives = 55/128 (42%), Gaps = 3/128 (2%) Frame = +2 Query: 2 SNGKLALLSVSDKMGLLSLAKSLLECGLQLIASCGTATAASERRPHSSRC--VGHHESTG 175 S K AL+S+SDK L SL L E G ++++ GTA+ ++ + H Sbjct: 69 SGEKQALISLSDKRDLASLGNGLQELGYTIVSTGGTASTLENAGVSVTKVEKLTHFPEML 128 Query: 176 DARRSGENFTSSGTCWDLSSIMRL*PEDMKRQMYEMISLVVCNLYPFVQTVSTL-DVTVA 352 D R + G + E + +VV NLYPF + V+ ++ Sbjct: 129 DGRVKTLHPNIHGGILARRDVEHH-MEALNEHGIGTFDVVVVNLYPFYEKVTAPGGISFE 187 Query: 353 DAVDNIDI 376 D ++NIDI Sbjct: 188 DGIENIDI 195 >At2g45150.3 68415.m05621 phosphatidate cytidylyltransferase family protein contains Pfam profile: PF01148 phosphatidate cytidylyltransferase Length = 382 Score = 29.1 bits (62), Expect = 2.0 Identities = 15/36 (41%), Positives = 17/36 (47%) Frame = -1 Query: 217 CTAGCKVFTRPPSISGALVMSDTS*TVRPAFRSGRG 110 CT C+ FTR P I L +P FRSG G Sbjct: 14 CTCPCRSFTRKPLIRPPLSGLRLPGDTKPLFRSGLG 49 >At2g45150.2 68415.m05619 phosphatidate cytidylyltransferase family protein contains Pfam profile: PF01148 phosphatidate cytidylyltransferase Length = 287 Score = 29.1 bits (62), Expect = 2.0 Identities = 15/36 (41%), Positives = 17/36 (47%) Frame = -1 Query: 217 CTAGCKVFTRPPSISGALVMSDTS*TVRPAFRSGRG 110 CT C+ FTR P I L +P FRSG G Sbjct: 14 CTCPCRSFTRKPLIRPPLSGLRLPGDTKPLFRSGLG 49 >At2g45150.1 68415.m05620 phosphatidate cytidylyltransferase family protein contains Pfam profile: PF01148 phosphatidate cytidylyltransferase Length = 391 Score = 29.1 bits (62), Expect = 2.0 Identities = 15/36 (41%), Positives = 17/36 (47%) Frame = -1 Query: 217 CTAGCKVFTRPPSISGALVMSDTS*TVRPAFRSGRG 110 CT C+ FTR P I L +P FRSG G Sbjct: 14 CTCPCRSFTRKPLIRPPLSGLRLPGDTKPLFRSGLG 49 >At3g48950.1 68416.m05347 glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein weak similarity to SP|P27644 Polygalacturonase (EC 3.2.1.15) (Pectinase) {Agrobacterium tumefaciens}; contains PF00295: Glycosyl hydrolases family 28 Length = 469 Score = 28.3 bits (60), Expect = 3.5 Identities = 14/44 (31%), Positives = 21/44 (47%) Frame = +1 Query: 13 TSSSQRFRQDGSTLVSKEPVGMWPAVDCQLRYRHGRFGTPASQF 144 TS F Q G+T+++ + WP + Y GR GT +F Sbjct: 97 TSHFTLFIQRGATILASQDESEWPVIAPLPSYGKGRDGTGTGRF 140 >At2g32560.1 68415.m03977 F-box family protein contains Pfam PF00646: F-box domain; similar to SKP1 interacting partner 2 (SKIP2) TIGR_Ath1:At5g67250 Length = 371 Score = 28.3 bits (60), Expect = 3.5 Identities = 15/45 (33%), Positives = 21/45 (46%) Frame = +2 Query: 29 VSDKMGLLSLAKSLLECGLQLIASCGTATAASERRPHSSRCVGHH 163 V D+M +L L + L+C L L+ G + A RCV H Sbjct: 56 VIDQMSVLDLPELALDCILDLLPPSGLCSMARVCSSLRERCVSDH 100 >At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DNA topoisomerase II / DNA gyrase (TOP2) identical to SP|P30182 DNA topoisomerase II (EC 5.99.1.3) {Arabidopsis thaliana} Length = 1473 Score = 27.1 bits (57), Expect = 8.2 Identities = 14/38 (36%), Positives = 20/38 (52%) Frame = -3 Query: 200 SFHPTSEHLRCSRDVRHILNCEAGVPKRPWRYRNWQST 87 +F P +VR +LN E+ VP PW YR ++ T Sbjct: 851 TFIPNYNPREIVANVRRLLNGESMVPMDPW-YRGFKGT 887 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,632,228 Number of Sequences: 28952 Number of extensions: 233394 Number of successful extensions: 659 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 648 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 659 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1023490624 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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