SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= tesV0420.Seq
         (547 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g35040.1 68415.m04299 AICARFT/IMPCHase bienzyme family protei...    50   1e-06
At2g45150.3 68415.m05621 phosphatidate cytidylyltransferase fami...    29   2.0  
At2g45150.2 68415.m05619 phosphatidate cytidylyltransferase fami...    29   2.0  
At2g45150.1 68415.m05620 phosphatidate cytidylyltransferase fami...    29   2.0  
At3g48950.1 68416.m05347 glycoside hydrolase family 28 protein /...    28   3.5  
At2g32560.1 68415.m03977 F-box family protein contains Pfam PF00...    28   3.5  
At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DN...    27   8.2  

>At2g35040.1 68415.m04299 AICARFT/IMPCHase bienzyme family protein
           similar to SP|P12048 Bifunctional purine biosynthesis
           protein purH [Includes:
           Phosphoribosylaminoimidazolecarboxamide
           formyltransferase (EC 2.1.2.3) (AICAR transformylase);
           IMP cyclohydrolase (EC 3.5.4.10) (Inosinicase) (IMP
           synthetase) (ATIC)] {Bacillus subtilis}; contains Pfam
           profiles PF01808: AICARFT/IMPCHase bienzyme, PF02142:
           MGS-like domain
          Length = 596

 Score = 50.0 bits (114), Expect = 1e-06
 Identities = 22/35 (62%), Positives = 26/35 (74%)
 Frame = +3

Query: 132 GLTVQDVSDITRAPEMLGGRVKTLHPAVHAGILAR 236
           G++V  V  +T  PEML GRVKTLHP +H GILAR
Sbjct: 112 GVSVTKVEKLTHFPEMLDGRVKTLHPNIHGGILAR 146



 Score = 40.7 bits (91), Expect = 6e-04
 Identities = 35/128 (27%), Positives = 55/128 (42%), Gaps = 3/128 (2%)
 Frame = +2

Query: 2   SNGKLALLSVSDKMGLLSLAKSLLECGLQLIASCGTATAASERRPHSSRC--VGHHESTG 175
           S  K AL+S+SDK  L SL   L E G  ++++ GTA+         ++   + H     
Sbjct: 69  SGEKQALISLSDKRDLASLGNGLQELGYTIVSTGGTASTLENAGVSVTKVEKLTHFPEML 128

Query: 176 DARRSGENFTSSGTCWDLSSIMRL*PEDMKRQMYEMISLVVCNLYPFVQTVSTL-DVTVA 352
           D R    +    G       +     E +         +VV NLYPF + V+    ++  
Sbjct: 129 DGRVKTLHPNIHGGILARRDVEHH-MEALNEHGIGTFDVVVVNLYPFYEKVTAPGGISFE 187

Query: 353 DAVDNIDI 376
           D ++NIDI
Sbjct: 188 DGIENIDI 195


>At2g45150.3 68415.m05621 phosphatidate cytidylyltransferase family
           protein contains Pfam profile: PF01148 phosphatidate
           cytidylyltransferase
          Length = 382

 Score = 29.1 bits (62), Expect = 2.0
 Identities = 15/36 (41%), Positives = 17/36 (47%)
 Frame = -1

Query: 217 CTAGCKVFTRPPSISGALVMSDTS*TVRPAFRSGRG 110
           CT  C+ FTR P I   L         +P FRSG G
Sbjct: 14  CTCPCRSFTRKPLIRPPLSGLRLPGDTKPLFRSGLG 49


>At2g45150.2 68415.m05619 phosphatidate cytidylyltransferase family
           protein contains Pfam profile: PF01148 phosphatidate
           cytidylyltransferase
          Length = 287

 Score = 29.1 bits (62), Expect = 2.0
 Identities = 15/36 (41%), Positives = 17/36 (47%)
 Frame = -1

Query: 217 CTAGCKVFTRPPSISGALVMSDTS*TVRPAFRSGRG 110
           CT  C+ FTR P I   L         +P FRSG G
Sbjct: 14  CTCPCRSFTRKPLIRPPLSGLRLPGDTKPLFRSGLG 49


>At2g45150.1 68415.m05620 phosphatidate cytidylyltransferase family
           protein contains Pfam profile: PF01148 phosphatidate
           cytidylyltransferase
          Length = 391

 Score = 29.1 bits (62), Expect = 2.0
 Identities = 15/36 (41%), Positives = 17/36 (47%)
 Frame = -1

Query: 217 CTAGCKVFTRPPSISGALVMSDTS*TVRPAFRSGRG 110
           CT  C+ FTR P I   L         +P FRSG G
Sbjct: 14  CTCPCRSFTRKPLIRPPLSGLRLPGDTKPLFRSGLG 49


>At3g48950.1 68416.m05347 glycoside hydrolase family 28 protein /
           polygalacturonase (pectinase) family protein weak
           similarity to SP|P27644 Polygalacturonase (EC 3.2.1.15)
           (Pectinase) {Agrobacterium tumefaciens}; contains
           PF00295: Glycosyl hydrolases family 28
          Length = 469

 Score = 28.3 bits (60), Expect = 3.5
 Identities = 14/44 (31%), Positives = 21/44 (47%)
 Frame = +1

Query: 13  TSSSQRFRQDGSTLVSKEPVGMWPAVDCQLRYRHGRFGTPASQF 144
           TS    F Q G+T+++ +    WP +     Y  GR GT   +F
Sbjct: 97  TSHFTLFIQRGATILASQDESEWPVIAPLPSYGKGRDGTGTGRF 140


>At2g32560.1 68415.m03977 F-box family protein contains Pfam
           PF00646: F-box domain; similar to SKP1 interacting
           partner 2 (SKIP2) TIGR_Ath1:At5g67250
          Length = 371

 Score = 28.3 bits (60), Expect = 3.5
 Identities = 15/45 (33%), Positives = 21/45 (46%)
 Frame = +2

Query: 29  VSDKMGLLSLAKSLLECGLQLIASCGTATAASERRPHSSRCVGHH 163
           V D+M +L L +  L+C L L+   G  + A        RCV  H
Sbjct: 56  VIDQMSVLDLPELALDCILDLLPPSGLCSMARVCSSLRERCVSDH 100


>At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DNA
           topoisomerase II / DNA gyrase (TOP2) identical to
           SP|P30182 DNA topoisomerase II (EC 5.99.1.3)
           {Arabidopsis thaliana}
          Length = 1473

 Score = 27.1 bits (57), Expect = 8.2
 Identities = 14/38 (36%), Positives = 20/38 (52%)
 Frame = -3

Query: 200 SFHPTSEHLRCSRDVRHILNCEAGVPKRPWRYRNWQST 87
           +F P         +VR +LN E+ VP  PW YR ++ T
Sbjct: 851 TFIPNYNPREIVANVRRLLNGESMVPMDPW-YRGFKGT 887


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,632,228
Number of Sequences: 28952
Number of extensions: 233394
Number of successful extensions: 659
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 648
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 659
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1023490624
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -