BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesV0392.Seq (827 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomy... 127 2e-30 SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 112 7e-26 SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar... 70 4e-13 SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharo... 58 1e-09 SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2||... 38 0.002 SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 35 0.012 SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr 1||... 35 0.012 SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|... 34 0.028 SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr 1|||... 29 1.1 SPAPB1A10.02 |||chromosome segregation protein |Schizosaccharomy... 28 1.4 SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr 1||... 28 1.4 SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 27 3.2 SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|ch... 27 4.3 SPAC3A11.11c |||pyridoxal reductase |Schizosaccharomyces pombe|c... 27 4.3 SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ... 26 5.7 SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 26 7.5 SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15 |Sc... 26 7.5 >SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomyces pombe|chr 2|||Manual Length = 180 Score = 127 bits (306), Expect = 2e-30 Identities = 68/138 (49%), Positives = 85/138 (61%) Frame = +3 Query: 255 YKLQVGEVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDS 434 YKL++GE+VTTIPTIGFNVE V Y+N+ F VWD+GGQ IRP WR Y+ NT II+VVDS Sbjct: 35 YKLKLGEIVTTIPTIGFNVETVEYRNISFTVWDVGGQDKIRPLWRHYFQNTQGIIFVVDS 94 Query: 435 ADRDRIGISKDELVHMLREEELANAYSLF*PTNRTWPDV*Q*PRYTRPWGLDALRDRTFP 614 DR+RI + +EL ML E+EL +A L + P+ T GL +LR R Sbjct: 95 NDRERISEAHEELQRMLNEDELRDALLLVFANKQDLPNAMNAAEITDKLGLHSLRHR--- 151 Query: 615 XL*DFSRQRRXARSGDGL 668 + Q A SGDGL Sbjct: 152 ---QWYIQATCATSGDGL 166 Score = 43.6 bits (98), Expect = 3e-05 Identities = 20/30 (66%), Positives = 23/30 (76%) Frame = +1 Query: 178 FRGLLGAREMRILILGLDGAGKTTILINCK 267 F+ L G REMRIL++GLD AGKTTIL K Sbjct: 9 FQSLFGKREMRILMVGLDAAGKTTILYKLK 38 >SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 112 bits (269), Expect = 7e-26 Identities = 59/138 (42%), Positives = 82/138 (59%) Frame = +3 Query: 255 YKLQVGEVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDS 434 YKL++ + V TIPT+GFNVE VTYKN+KF VWD+GGQ IRP WR Y+ T +I+VVDS Sbjct: 39 YKLKLNQSVVTIPTVGFNVETVTYKNIKFNVWDVGGQDKIRPLWRHYFTGTKGLIFVVDS 98 Query: 435 ADRDRIGISKDELVHMLREEELANAYSLF*PTNRTWPDV*Q*PRYTRPWGLDALRDRTFP 614 AD +RI ++ EL ++ + E+ + L + P + T LD L+DR Sbjct: 99 ADSNRISEARQELHRIISDREMRDCLLLVLANKQDLPGALSPAQITDVLQLDKLKDRL-- 156 Query: 615 XL*DFSRQRRXARSGDGL 668 ++ Q A +GDGL Sbjct: 157 ----WNVQPTCALTGDGL 170 Score = 39.5 bits (88), Expect = 6e-04 Identities = 20/35 (57%), Positives = 22/35 (62%) Frame = +1 Query: 163 GLFSYFRGLLGAREMRILILGLDGAGKTTILINCK 267 G F L +EMRIL+LGLD AGKTTIL K Sbjct: 8 GFSKPFSRLFSNKEMRILMLGLDAAGKTTILYKLK 42 >SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosaccharomyces pombe|chr 1|||Manual Length = 186 Score = 70.1 bits (164), Expect = 4e-13 Identities = 29/70 (41%), Positives = 48/70 (68%) Frame = +3 Query: 291 PTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDRIGISKDE 470 PT GF + + + L+F +WD+GGQ ++R +W+ Y+ +T+AII+VVDS D R+ ++ Sbjct: 46 PTFGFQIRTLEVEGLRFTIWDIGGQKTLRNFWKNYFESTEAIIWVVDSLDDLRLEECRNT 105 Query: 471 LVHMLREEEL 500 L +L EE+L Sbjct: 106 LQELLVEEKL 115 Score = 35.9 bits (79), Expect = 0.007 Identities = 16/22 (72%), Positives = 19/22 (86%) Frame = +1 Query: 190 LGAREMRILILGLDGAGKTTIL 255 L RE+R+L+LGLD AGKTTIL Sbjct: 12 LKEREVRVLLLGLDNAGKTTIL 33 >SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharomyces pombe|chr 2|||Manual Length = 190 Score = 58.4 bits (135), Expect = 1e-09 Identities = 28/71 (39%), Positives = 41/71 (57%) Frame = +3 Query: 291 PTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDRIGISKDE 470 PT+ E++ N++F +DLGG R WR Y+ + I+Y+VD D +R+ SK E Sbjct: 50 PTLHPTSEELAIGNVRFTTFDLGGHQQARRLWRDYFPEVNGIVYLVDCCDFERLSESKAE 109 Query: 471 LVHMLREEELA 503 L +L EELA Sbjct: 110 LDALLAMEELA 120 Score = 28.7 bits (61), Expect = 1.1 Identities = 13/26 (50%), Positives = 18/26 (69%) Frame = +1 Query: 199 REMRILILGLDGAGKTTILINCKSVR 276 + ++L LGLD AGKTT+L K+ R Sbjct: 19 KHAKMLFLGLDNAGKTTLLHMLKNDR 44 >SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 203 Score = 37.9 bits (84), Expect = 0.002 Identities = 21/57 (36%), Positives = 27/57 (47%), Gaps = 4/57 (7%) Frame = +3 Query: 288 IPTIGFNVEQVTY----KNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRD 446 I TIG + + T+ K +K Q+WD GQ R YY II V D D+D Sbjct: 38 ISTIGVDFKIRTFELEGKTVKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQD 94 >SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 35.1 bits (77), Expect = 0.012 Identities = 16/52 (30%), Positives = 30/52 (57%) Frame = +3 Query: 294 TIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDR 449 T+G + T +L+ +++D+GGQ + R W + N ++II+ V D D+ Sbjct: 179 TLGISEISFTLDHLQIRMFDVGGQRTERRKWIYCFENVNSIIFCVSLNDYDK 230 >SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 35.1 bits (77), Expect = 0.012 Identities = 20/68 (29%), Positives = 31/68 (45%), Gaps = 1/68 (1%) Frame = +3 Query: 246 DNSYKLQVG-EVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIY 422 D+ VG + + I ++G +Q K +K Q+WD GQ R R YY + Sbjct: 35 DDQVSHTVGIDFASRIISVGMGNQQ---KRIKLQIWDTAGQEKFRSVARNYYRGAAGAVL 91 Query: 423 VVDSADRD 446 V D ++D Sbjct: 92 VYDVTNKD 99 >SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 33.9 bits (74), Expect = 0.028 Identities = 18/57 (31%), Positives = 26/57 (45%), Gaps = 2/57 (3%) Frame = +3 Query: 279 VTTIPTIGFNVEQVTY--KNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADR 443 +TTI I F + + K +K Q+WD GQ R YY I+ + D D+ Sbjct: 39 ITTIG-IDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGILLLYDVTDK 94 >SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 211 Score = 28.7 bits (61), Expect = 1.1 Identities = 13/54 (24%), Positives = 24/54 (44%) Frame = +3 Query: 330 NLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDRIGISKDELVHMLRE 491 ++K ++WD GQ + YY N + I V D + +K + + R+ Sbjct: 63 SVKLEIWDTAGQERYKSLAPMYYRNANCAIVVYDITQAASLEKAKSWIKELQRQ 116 >SPAPB1A10.02 |||chromosome segregation protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 336 Score = 28.3 bits (60), Expect = 1.4 Identities = 14/31 (45%), Positives = 17/31 (54%) Frame = +1 Query: 523 SQQTGHGRMSDSSRGTPGPGAWTPCAIEPFQ 615 S G+ + DSS G PG A +P IE FQ Sbjct: 105 SASNGYEKKLDSSIGNPGEKAVSPVHIEDFQ 135 >SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr 1|||Manual Length = 214 Score = 28.3 bits (60), Expect = 1.4 Identities = 12/35 (34%), Positives = 15/35 (42%) Frame = +3 Query: 327 KNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVD 431 K +K Q+WD GQ R YY + V D Sbjct: 57 KKIKAQIWDTAGQERYRAITSAYYRGAVGALIVYD 91 >SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces pombe|chr 2|||Manual Length = 1142 Score = 27.1 bits (57), Expect = 3.2 Identities = 10/31 (32%), Positives = 19/31 (61%) Frame = -1 Query: 464 FRYSYSIPVS*VYNIYYCICVAIVTSPIRPN 372 F ++Y++ S VYN + +C + T+P + N Sbjct: 494 FDFTYNLSESEVYNTVFNLCDELYTTPCKKN 524 >SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|chr 1|||Manual Length = 198 Score = 26.6 bits (56), Expect = 4.3 Identities = 13/59 (22%), Positives = 28/59 (47%), Gaps = 2/59 (3%) Frame = +3 Query: 297 IGFNVEQVTYKN--LKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDRIGISKD 467 + F + ++ +N + F +WDLGGQ + AI+++ D + + + K+ Sbjct: 45 VNFMEKTISIRNTEITFSIWDLGGQREFVNMLPMVCNDAVAILFMFDLSRKSTLNSIKE 103 >SPAC3A11.11c |||pyridoxal reductase |Schizosaccharomyces pombe|chr 1|||Manual Length = 334 Score = 26.6 bits (56), Expect = 4.3 Identities = 13/42 (30%), Positives = 20/42 (47%) Frame = +1 Query: 649 LDQAMDWLSNALQARELTFTVGVLYEEXPRPASPXNEPCVRG 774 ++ ++ S L + V L + P PASP +EP V G Sbjct: 287 IEASLGSFSKVLDTYQFAEVVSCLEKTLPPPASPNSEPQVTG 328 >SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 407 Score = 26.2 bits (55), Expect = 5.7 Identities = 9/21 (42%), Positives = 16/21 (76%) Frame = +1 Query: 193 GAREMRILILGLDGAGKTTIL 255 G ++++L+LG +GKTTI+ Sbjct: 71 GGNDIKVLLLGAGDSGKTTIM 91 >SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9|Schizosaccharomyces pombe|chr 1|||Manual Length = 1223 Score = 25.8 bits (54), Expect = 7.5 Identities = 13/34 (38%), Positives = 19/34 (55%) Frame = -1 Query: 296 RRYSGHDLTDLQFIRIVVFPAPSNPSIKIRISLA 195 R S H+L+ + FIR + P P++ KI LA Sbjct: 1107 RNNSSHELSLVAFIREMAMPVPNDEFKKISTILA 1140 >SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15 |Schizosaccharomyces pombe|chr 3|||Manual Length = 785 Score = 25.8 bits (54), Expect = 7.5 Identities = 15/50 (30%), Positives = 21/50 (42%) Frame = -1 Query: 641 PLTAEVLKXWKGSIAQGVQAPGPGVPRLLSDIRPCPVCWLKQRVCVR*FF 492 P+ E + + IA P PR +S + CP+C VR FF Sbjct: 427 PMIVETQEEYDHYIAPYWNFPSEPFPRTMSFSKMCPLCCTTLSKFVRHFF 476 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,369,210 Number of Sequences: 5004 Number of extensions: 70779 Number of successful extensions: 187 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 175 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 185 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 406444570 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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