BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesV0366.Seq (748 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC3H7.15 |hhp1||serine/threonine protein kinase Hhp1|Schizosac... 152 4e-38 SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos... 141 8e-35 SPBP35G2.05c |cki2||serine/threonine protein kinase Cki2|Schizos... 107 2e-24 SPAC1805.05 |cki3||serine/threonine protein kinase Cki3|Schizosa... 101 1e-22 SPBC1347.06c |cki1||serine/threonine protein kinase Cki1|Schizos... 96 5e-21 SPBC8D2.19 |mde3||serine/threonine protein kinase Mde3|Schizosac... 33 0.057 SPAC890.03 |ppk16|mug92|serine/threonine protein kinase Ppk16 |S... 29 0.53 SPAC3C7.06c |pit1||serine/threonine protein kinase Pit1|Schizosa... 29 0.53 SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch... 29 0.93 SPACUNK12.02c |cmk1||calcium/calmodulin-dependent protein kinase... 28 1.2 SPBC1718.07c |zfs1|moc4|transcription factor Zfs1 |Schizosacchar... 28 1.6 SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 27 2.1 SPBC32C12.03c |ppk25||serine/threonine protein kinase Ppk25 |Sch... 27 3.8 SPAC17H9.07 |||signal recognition particle subunit Srp21 |Schizo... 27 3.8 SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizos... 27 3.8 SPBC776.12c |hsk1||serine/threonine protein kinase Hsk1|Schizosa... 27 3.8 SPCC320.13c |ark1|aim1, SPCC330.16|aurora-B kinase Ark1|Schizosa... 27 3.8 SPBPB10D8.02c |||arylsulfatase |Schizosaccharomyces pombe|chr 2|... 27 3.8 SPAC19E9.02 |fin1||serine/threonine protein kinase Fin1|Schizosa... 26 5.0 SPAC222.08c |||imidazoleglycerol-phosphate synthase |Schizosacch... 26 6.6 SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 26 6.6 SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase Byr2... 25 8.7 SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 25 8.7 >SPBC3H7.15 |hhp1||serine/threonine protein kinase Hhp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 365 Score = 152 bits (369), Expect = 4e-38 Identities = 75/137 (54%), Positives = 97/137 (70%), Gaps = 1/137 (0%) Frame = +2 Query: 257 TGEEVAVKVESTKARHPQLLYESRVYXMLQGGVGIPHIRWYGYEREYNILVMDLLGPSLE 436 +GEEVA+K+EST+A+HPQL YE RVY +L GGVGIP +RW+G E +YN +VMDLLGPSLE Sbjct: 33 SGEEVAIKLESTRAKHPQLEYEYRVYRILSGGVGIPFVRWFGVECDYNAMVMDLLGPSLE 92 Query: 437 XLFNFCTRQFTIKTVLMLADQMAERVEFLHWQ-VLHSPRHSXRTTS*WASAGHFXXLYMI 613 LFNFC R+F++KTVL+LADQ+ R+EF+H + LH G+ + +I Sbjct: 93 DLFNFCNRKFSLKTVLLLADQLISRIEFIHSKSFLHRDIKPDNFLMGIGKRGN--QVNII 150 Query: 614 DFGLAKKYXGLRTRAXI 664 DFGLAKKY +T I Sbjct: 151 DFGLAKKYRDHKTHLHI 167 Score = 41.1 bits (92), Expect = 2e-04 Identities = 19/46 (41%), Positives = 25/46 (54%) Frame = +3 Query: 171 EVLISGKYKVSKKIGGGSFGDIYLAINIELARRWQ*KLNLQRPDTP 308 ++ I KY++ +KIG GSFGDIYL N+ KL R P Sbjct: 4 DLRIGNKYRIGRKIGSGSFGDIYLGTNVVSGEEVAIKLESTRAKHP 49 >SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 400 Score = 141 bits (342), Expect = 8e-35 Identities = 71/135 (52%), Positives = 90/135 (66%) Frame = +2 Query: 260 GEEVAVKVESTKARHPQLLYESRVYXMLQGGVGIPHIRWYGYEREYNILVMDLLGPSLEX 439 GE+VAVK+E KARH QL YE RVY +L+G +GIP IRW+G YN +VMDLLGPSLE Sbjct: 35 GEQVAVKLEPLKARHHQLEYEFRVYNILKGNIGIPTIRWFGVTNSYNAMVMDLLGPSLED 94 Query: 440 LFNFCTRQFTIKTVLMLADQMAERVEFLHWQVLHSPRHSXRTTS*WASAGHFXXLYMIDF 619 LF +C R+FT+KTVL+LADQ+ R+E++H + S H + H + MIDF Sbjct: 95 LFCYCGRKFTLKTVLLLADQLISRIEYVHSK---SFLHRDIKPDNFLMKKHSNVVTMIDF 151 Query: 620 GLAKKYXGLRTRAXI 664 GLAKKY +T I Sbjct: 152 GLAKKYRDFKTHVHI 166 Score = 38.7 bits (86), Expect = 9e-04 Identities = 14/27 (51%), Positives = 20/27 (74%) Frame = +3 Query: 171 EVLISGKYKVSKKIGGGSFGDIYLAIN 251 ++ I KY++ +KIG GSFG IYL +N Sbjct: 5 DIKIGNKYRIGRKIGSGSFGQIYLGLN 31 >SPBP35G2.05c |cki2||serine/threonine protein kinase Cki2|Schizosaccharomyces pombe|chr 2|||Manual Length = 435 Score = 107 bits (256), Expect = 2e-24 Identities = 56/141 (39%), Positives = 84/141 (59%), Gaps = 1/141 (0%) Frame = +2 Query: 269 VAVKVESTKARHPQLLYESRVYXMLQGGVGIPHIRWYGYEREYNILVMDLLGPSLEXLFN 448 +A+K E K+ PQL E R Y +L G GIP++ ++G E +NILV+DLLGPSLE LF Sbjct: 38 IAIKFEPKKSEAPQLRDEYRTYKLLVGNAGIPNVYYFGQEGLHNILVIDLLGPSLEDLFE 97 Query: 449 FCTRQFTIKTVLMLADQMAERVEFLHWQ-VLHSPRHSXRTTS*WASAGHFXXLYMIDFGL 625 +C R+F++KTV M A QM RV+ +H + +++ S+ + +YM+DFG+ Sbjct: 98 WCGRRFSVKTVAMTAKQMLSRVQTIHEKNLVYRDIKPDNFLIGRPSSRNANMVYMVDFGM 157 Query: 626 AKKYXGLRTRAXISLXRRPEI 688 AK Y +T+ I R + Sbjct: 158 AKYYRDPKTKQHIPYSERKSL 178 Score = 27.9 bits (59), Expect = 1.6 Identities = 11/21 (52%), Positives = 16/21 (76%) Frame = +3 Query: 192 YKVSKKIGGGSFGDIYLAINI 254 Y+V +KIG GSFG I+ +N+ Sbjct: 12 YRVGRKIGEGSFGVIFDGMNL 32 >SPAC1805.05 |cki3||serine/threonine protein kinase Cki3|Schizosaccharomyces pombe|chr 1|||Manual Length = 439 Score = 101 bits (241), Expect = 1e-22 Identities = 53/131 (40%), Positives = 79/131 (60%), Gaps = 1/131 (0%) Frame = +2 Query: 263 EEVAVKVESTKARHPQLLYESRVYXMLQGGVGIPHIRWYGYEREYNILVMDLLGPSLEXL 442 + +A+K ES K+ PQL E Y +L G GIP + +YG E YN+LVMDLLGPSLE L Sbjct: 39 QPIALKFESRKSEVPQLRDEYLTYKLLMGLPGIPSVYYYGQEGMYNLLVMDLLGPSLEDL 98 Query: 443 FNFCTRQFTIKTVLMLADQMAERVEFLHWQ-VLHSPRHSXRTTS*WASAGHFXXLYMIDF 619 F++C R+F+ KTV M+A QM R++ +H + ++ + + +Y +DF Sbjct: 99 FDYCGRRFSPKTVAMIAKQMITRIQSVHERHFIYRDIKPDNFLIGFPGSKTENVIYAVDF 158 Query: 620 GLAKKYXGLRT 652 G+AK+Y +T Sbjct: 159 GMAKQYRDPKT 169 Score = 29.9 bits (64), Expect = 0.40 Identities = 11/21 (52%), Positives = 16/21 (76%) Frame = +3 Query: 192 YKVSKKIGGGSFGDIYLAINI 254 Y+V KKIG GSFG ++ +N+ Sbjct: 15 YRVGKKIGEGSFGMLFQGVNL 35 >SPBC1347.06c |cki1||serine/threonine protein kinase Cki1|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 95.9 bits (228), Expect = 5e-21 Identities = 51/135 (37%), Positives = 81/135 (60%), Gaps = 1/135 (0%) Frame = +2 Query: 263 EEVAVKVESTKARHPQLLYESRVYXMLQGGVGIPHIRWYGYEREYNILVMDLLGPSLEXL 442 ++VA+K E ++ PQL E R Y +L G GIP++ ++G E +NILV+DLLGPSLE L Sbjct: 36 QQVAIKFEPRRSDAPQLRDEYRTYKLLAGCTGIPNVYYFGQEGLHNILVIDLLGPSLEDL 95 Query: 443 FNFCTRQFTIKTVLMLADQMAERVEFLHWQ-VLHSPRHSXRTTS*WASAGHFXXLYMIDF 619 + C R+F++KTV M A QM RV+ +H + +++ ++ + +Y++DF Sbjct: 96 LDLCGRKFSVKTVAMAAKQMLARVQSIHEKSLVYRDIKPDNFLIGRPNSKNANMIYVVDF 155 Query: 620 GLAKKYXGLRTRAXI 664 G+ K Y T+ I Sbjct: 156 GMVKFYRDPVTKQHI 170 Score = 29.9 bits (64), Expect = 0.40 Identities = 15/39 (38%), Positives = 22/39 (56%) Frame = +3 Query: 192 YKVSKKIGGGSFGDIYLAINIELARRWQ*KLNLQRPDTP 308 YKV ++IG GSFG I+ N+ ++ K +R D P Sbjct: 12 YKVGRRIGEGSFGVIFEGTNLLNNQQVAIKFEPRRSDAP 50 >SPBC8D2.19 |mde3||serine/threonine protein kinase Mde3|Schizosaccharomyces pombe|chr 2|||Manual Length = 559 Score = 32.7 bits (71), Expect = 0.057 Identities = 12/26 (46%), Positives = 19/26 (73%) Frame = +3 Query: 168 SEVLISGKYKVSKKIGGGSFGDIYLA 245 ++V+ +Y V +K+G GSFG +YLA Sbjct: 13 TQVIFEDRYLVKQKLGDGSFGTVYLA 38 >SPAC890.03 |ppk16|mug92|serine/threonine protein kinase Ppk16 |Schizosaccharomyces pombe|chr 1|||Manual Length = 672 Score = 29.5 bits (63), Expect = 0.53 Identities = 10/23 (43%), Positives = 16/23 (69%) Frame = +3 Query: 186 GKYKVSKKIGGGSFGDIYLAINI 254 G Y++ +G GSFG +YLA ++ Sbjct: 29 GTYRIESVVGEGSFGKVYLAAHL 51 >SPAC3C7.06c |pit1||serine/threonine protein kinase Pit1|Schizosaccharomyces pombe|chr 1|||Manual Length = 650 Score = 29.5 bits (63), Expect = 0.53 Identities = 12/27 (44%), Positives = 17/27 (62%) Frame = +3 Query: 165 RSEVLISGKYKVSKKIGGGSFGDIYLA 245 R + I Y V +K+G G+FG +YLA Sbjct: 27 RKCISIDEVYNVVRKVGDGTFGSVYLA 53 >SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1318 Score = 28.7 bits (61), Expect = 0.93 Identities = 10/24 (41%), Positives = 17/24 (70%) Frame = -3 Query: 116 HKVKIMIMKSQITHNPNGNRGKIL 45 HKV ++IM+S I H GN+ +++ Sbjct: 830 HKVHVLIMRSWIQHLDEGNKSEVI 853 >SPACUNK12.02c |cmk1||calcium/calmodulin-dependent protein kinase Cmk1|Schizosaccharomyces pombe|chr 1|||Manual Length = 335 Score = 28.3 bits (60), Expect = 1.2 Identities = 11/36 (30%), Positives = 23/36 (63%) Frame = +3 Query: 177 LISGKYKVSKKIGGGSFGDIYLAINIELARRWQ*KL 284 L+ KY+V + +GGG++ + A++IE + + K+ Sbjct: 26 LLPCKYRVGRVLGGGTYATVREAVHIETNKMYAAKI 61 >SPBC1718.07c |zfs1|moc4|transcription factor Zfs1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 404 Score = 27.9 bits (59), Expect = 1.6 Identities = 12/30 (40%), Positives = 16/30 (53%) Frame = +1 Query: 409 HGPAGPLARGSLQLLHPPVHDKNRSNACRS 498 +G A G+ +L PP H K +S CRS Sbjct: 343 YGSKCQFAHGNQELKEPPRHPKYKSERCRS 372 >SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1436 Score = 27.5 bits (58), Expect = 2.1 Identities = 12/32 (37%), Positives = 21/32 (65%) Frame = +3 Query: 162 SRSEVLISGKYKVSKKIGGGSFGDIYLAINIE 257 S+S + I +++ IG GSFG +Y A+N++ Sbjct: 1112 SKSNITI--RWQQGGLIGSGSFGTVYRAVNLD 1141 >SPBC32C12.03c |ppk25||serine/threonine protein kinase Ppk25 |Schizosaccharomyces pombe|chr 2|||Manual Length = 423 Score = 26.6 bits (56), Expect = 3.8 Identities = 15/42 (35%), Positives = 21/42 (50%) Frame = +3 Query: 186 GKYKVSKKIGGGSFGDIYLAINIELARRWQ*KLNLQRPDTPN 311 G + + K IG GS G + L +NI + K+ P TPN Sbjct: 51 GNWIIKKTIGAGSMGKVKLVVNILTGEKAALKM---IPFTPN 89 >SPAC17H9.07 |||signal recognition particle subunit Srp21 |Schizosaccharomyces pombe|chr 1|||Manual Length = 120 Score = 26.6 bits (56), Expect = 3.8 Identities = 12/17 (70%), Positives = 13/17 (76%) Frame = -2 Query: 150 TTFLRI*YKTNAQSQNY 100 TT L I Y+TN QSQNY Sbjct: 24 TTKLSIKYRTNEQSQNY 40 >SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1338 Score = 26.6 bits (56), Expect = 3.8 Identities = 10/18 (55%), Positives = 13/18 (72%) Frame = +3 Query: 192 YKVSKKIGGGSFGDIYLA 245 YK+ K I G+FG +YLA Sbjct: 589 YKILKPISKGAFGSVYLA 606 >SPBC776.12c |hsk1||serine/threonine protein kinase Hsk1|Schizosaccharomyces pombe|chr 2|||Manual Length = 507 Score = 26.6 bits (56), Expect = 3.8 Identities = 11/35 (31%), Positives = 21/35 (60%) Frame = +3 Query: 162 SRSEVLISGKYKVSKKIGGGSFGDIYLAINIELAR 266 +R+ V + Y++ +KIG G+F +Y A ++ R Sbjct: 58 TRTFVELQENYRLIEKIGEGTFSSVYKAEDLHYGR 92 >SPCC320.13c |ark1|aim1, SPCC330.16|aurora-B kinase Ark1|Schizosaccharomyces pombe|chr 3|||Manual Length = 355 Score = 26.6 bits (56), Expect = 3.8 Identities = 9/20 (45%), Positives = 14/20 (70%) Frame = +3 Query: 186 GKYKVSKKIGGGSFGDIYLA 245 G +++ K +G G FG +YLA Sbjct: 87 GMFEIGKPLGKGKFGRVYLA 106 >SPBPB10D8.02c |||arylsulfatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 554 Score = 26.6 bits (56), Expect = 3.8 Identities = 12/40 (30%), Positives = 18/40 (45%) Frame = -1 Query: 394 FALVAVPSYVWYAYTPLEHXIHSALIEKLGVSGLCRFNFH 275 F ++ W +P IH+ IE+L G+ NFH Sbjct: 14 FLVIVADDLGWSDVSPFGSEIHTPNIERLAKEGVRLTNFH 53 >SPAC19E9.02 |fin1||serine/threonine protein kinase Fin1|Schizosaccharomyces pombe|chr 1|||Manual Length = 722 Score = 26.2 bits (55), Expect = 5.0 Identities = 11/17 (64%), Positives = 13/17 (76%) Frame = +3 Query: 189 KYKVSKKIGGGSFGDIY 239 KYK+ + IG GSFG IY Sbjct: 3 KYKILECIGHGSFGRIY 19 >SPAC222.08c |||imidazoleglycerol-phosphate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 25.8 bits (54), Expect = 6.6 Identities = 14/32 (43%), Positives = 20/32 (62%), Gaps = 2/32 (6%) Frame = +3 Query: 183 SGKYKVSKKIGGGSFGDIYL--AINIELARRW 272 +G +SKK GG F D YL A++IE+ R + Sbjct: 102 AGMILLSKKSRGGKFPDPYLLRAMDIEVTRNY 133 >SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 891 Score = 25.8 bits (54), Expect = 6.6 Identities = 13/42 (30%), Positives = 24/42 (57%) Frame = +3 Query: 159 RSRSEVLISGKYKVSKKIGGGSFGDIYLAINIELARRWQ*KL 284 R R +V+ G Y + K IG GS G + +A +++ ++ K+ Sbjct: 116 RERRKVI--GNYVLGKTIGAGSMGKVKVAHHLKTGEQFAIKI 155 >SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase Byr2|Schizosaccharomyces pombe|chr 2|||Manual Length = 659 Score = 25.4 bits (53), Expect = 8.7 Identities = 9/14 (64%), Positives = 11/14 (78%) Frame = +3 Query: 210 IGGGSFGDIYLAIN 251 IG GSFG +YL +N Sbjct: 400 IGSGSFGQVYLGMN 413 >SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1828 Score = 25.4 bits (53), Expect = 8.7 Identities = 14/43 (32%), Positives = 22/43 (51%) Frame = -3 Query: 305 GVGPL*IQLSLPPPRQFDVDSQIYITERSTTDLFRHLVFSRNQ 177 G PL +L L D D +I + ++ T+LFR ++ NQ Sbjct: 1396 GHSPLIFRLPLQRHNSRDADEEILVFTKAQTELFR-IISKSNQ 1437 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,005,811 Number of Sequences: 5004 Number of extensions: 59617 Number of successful extensions: 200 Number of sequences better than 10.0: 23 Number of HSP's better than 10.0 without gapping: 185 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 195 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 355273338 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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