Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesV0355.Seq
(752 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 122 7e-29
SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large... 84 2e-17
SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex lar... 82 1e-16
SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual 29 0.54
SPBC1289.02c |uap2||U2 snRNP-associated protein Uap2|Schizosacch... 29 0.71
SPAC27D7.14c |tpr1|SPAC637.02c|RNA polymerase II associated Paf1... 27 3.8
SPBC25H2.03 |||vacuolar protein involved in phosphoinositide met... 25 8.8
>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1752
Score = 122 bits (293), Expect = 7e-29
Identities = 58/85 (68%), Positives = 70/85 (82%)
Frame = +2
Query: 254 VGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEIINISKKPKAPSLTVFLTGG 433
VG LAAQS+GEPATQMTLNTFH+AGVSSKNVTLGVPRLKEI+N++K K PSLT++L
Sbjct: 1067 VGTLAAQSIGEPATQMTLNTFHYAGVSSKNVTLGVPRLKEILNVAKNIKTPSLTIYLMPW 1126
Query: 434 AARDAEKAKNVLCRLEHTTLRKVTA 508
A + + AKNV ++EHTTL VT+
Sbjct: 1127 IAANMDLAKNVQTQIEHTTLSTVTS 1151
Score = 98.3 bits (234), Expect = 1e-21
Identities = 45/84 (53%), Positives = 60/84 (71%)
Frame = +3
Query: 3 DLSPIKVIKGVSDLLKKCVIVAGDDRLSKQANENATLLFQCLVRSTLCTKFVSEEHRLSS 182
DL P +I G+++L+ K I G DR+++ NATLLFQ L+RS K V E+RL+
Sbjct: 983 DLLPSDIINGLNELIAKLTIFRGSDRITRDVQNNATLLFQILLRSKFAVKRVIMEYRLNK 1042
Query: 183 VAFEWLIGEIEARFQQAQVNPGEM 254
VAFEW++GE+EARFQQA V+PGEM
Sbjct: 1043 VAFEWIMGEVEARFQQAVVSPGEM 1066
Score = 48.4 bits (110), Expect = 1e-06
Identities = 19/35 (54%), Positives = 27/35 (77%)
Frame = +1
Query: 511 TAIYYDPDPQNTVIAEDQEFVNVYYEMPDFDPGEN 615
T I+YDPDPQ+TVI ED++FV ++ +PD + EN
Sbjct: 1153 TEIHYDPDPQDTVIEEDKDFVEAFFAIPDEEVEEN 1187
Score = 27.9 bits (59), Expect = 1.6
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = +3
Query: 633 RIELDRKRMTDKKLTWEQIA 692
R+ELDR +M DKKL+ +A
Sbjct: 1197 RLELDRAKMLDKKLSMSDVA 1216
>SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large
subunit Rpc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1405
Score = 83.8 bits (198), Expect = 2e-17
Identities = 46/85 (54%), Positives = 58/85 (68%)
Frame = +2
Query: 254 VGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEIINISKKPKAPSLTVFLTGG 433
VGA+ AQS+GEP TQMTL TFHFAGV+++ TLGVPR+KEIIN +K P +T L
Sbjct: 1047 VGAIGAQSIGEPGTQMTLKTFHFAGVAAQ-TTLGVPRIKEIINAAKTISTPIITGQLIND 1105
Query: 434 AARDAEKAKNVLCRLEHTTLRKVTA 508
RD A+ V R+E T L+ VT+
Sbjct: 1106 --RDERSARVVKGRIEKTYLKDVTS 1128
>SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex large
subunit Nuc1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 1689
Score = 81.8 bits (193), Expect = 1e-16
Identities = 40/76 (52%), Positives = 55/76 (72%), Gaps = 1/76 (1%)
Frame = +2
Query: 251 NVGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEII-NISKKPKAPSLTVFLT 427
+VG LA+QS+GEP+TQMTLNTFHFAG +KNVTLG+PRL+EII S + P++T+ L
Sbjct: 1193 SVGVLASQSIGEPSTQMTLNTFHFAGFGAKNVTLGIPRLREIIMTASANIQTPTMTLRLN 1252
Query: 428 GGAARDAEKAKNVLCR 475
G ++K + C+
Sbjct: 1253 DGV---SDKRASAFCK 1265
>SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual
Length = 674
Score = 29.5 bits (63), Expect = 0.54
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = -2
Query: 169 CSSDTNFVQSVDLTKHWKSNVAFSLACLDKR 77
CS D F +++ L + W +N AFSL L R
Sbjct: 273 CSGDGTFRKNLSLWREWSANSAFSLHPLQLR 303
>SPBC1289.02c |uap2||U2 snRNP-associated protein
Uap2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 367
Score = 29.1 bits (62), Expect = 0.71
Identities = 16/47 (34%), Positives = 22/47 (46%)
Frame = +2
Query: 314 FHFAGVSSKNVTLGVPRLKEIINISKKPKAPSLTVFLTGGAARDAEK 454
F GV +KN+ G PR+K PK +L VF + AE+
Sbjct: 130 FKKCGVIAKNIDNGTPRIKIYRTEDGTPKGDALIVFFRSESVELAEQ 176
>SPAC27D7.14c |tpr1|SPAC637.02c|RNA polymerase II associated Paf1
complex subunit Tpr1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1039
Score = 26.6 bits (56), Expect = 3.8
Identities = 11/27 (40%), Positives = 20/27 (74%)
Frame = +3
Query: 621 LGYWRIELDRKRMTDKKLTWEQIAEEN 701
+G +I++ + +T KLT+E+IAE+N
Sbjct: 364 VGIGQIQILQNDLTSAKLTFERIAEQN 390
>SPBC25H2.03 |||vacuolar protein involved in phosphoinositide
metabolism|Schizosaccharomyces pombe|chr 2|||Manual
Length = 811
Score = 25.4 bits (53), Expect = 8.8
Identities = 17/62 (27%), Positives = 31/62 (50%), Gaps = 2/62 (3%)
Frame = -3
Query: 618 EIFARIKVRHFVVNIDKFLVLCNNSILRIRVIINSRVAVTLRKVVCSNL--QRTFLAFSA 445
E+ A I + H VN+ FL R +NSR + +R+ +C+ + +R + +F+
Sbjct: 499 ELLAHIAISHKSVNLVPFLKSLLQMFAEDRKFLNSRGNLIIRQ-LCNYIEGERVYTSFAG 557
Query: 444 SL 439
L
Sbjct: 558 IL 559
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,118,817
Number of Sequences: 5004
Number of extensions: 63565
Number of successful extensions: 173
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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