BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesV0355.Seq (752 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_44922| Best HMM Match : RNA_pol_Rpb1_1 (HMM E-Value=0) 161 6e-40 SB_19734| Best HMM Match : RNA_pol_Rpb1_5 (HMM E-Value=0) 76 4e-14 SB_45318| Best HMM Match : RNA_pol_Rpb1_7 (HMM E-Value=0) 72 6e-13 SB_24568| Best HMM Match : No HMM Matches (HMM E-Value=.) 58 6e-09 SB_7831| Best HMM Match : RNA_pol_Rpb1_7 (HMM E-Value=0) 53 2e-07 SB_21907| Best HMM Match : RNA_pol_Rpb1_5 (HMM E-Value=1.3e-21) 44 1e-04 SB_33304| Best HMM Match : RNA_pol_Rpb1_1 (HMM E-Value=7.00649e-45) 44 1e-04 SB_47320| Best HMM Match : Ion_trans (HMM E-Value=2.7e-34) 31 1.3 SB_57150| Best HMM Match : ThiF (HMM E-Value=2.8e-35) 30 1.8 SB_26367| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.1 SB_17864| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.4 SB_4456| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.1 >SB_44922| Best HMM Match : RNA_pol_Rpb1_1 (HMM E-Value=0) Length = 1596 Score = 161 bits (391), Expect = 6e-40 Identities = 84/126 (66%), Positives = 92/126 (73%) Frame = +2 Query: 254 VGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEIINISKKPKAPSLTVFLTGG 433 VGALAAQSLGEPATQMTLNTFH+AGVS+KNVTLGVPRLKEIIN+SKKPK PSLTVFL G Sbjct: 1412 VGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKEIINVSKKPKTPSLTVFLIGQ 1471 Query: 434 AARDAEKAKNVLCRLEHTTLRKVTAXXXXXXXXXXXXXXXXXXKNLSMFTTKCLTLILAK 613 AARDAE AK VLCRLEHTTLRKVTA ++ + +A+ Sbjct: 1472 AARDAEMAKAVLCRLEHTTLRKVTANTAIYYDPDPQNTVIAEDQDFVNVYYEMPDFDVAR 1531 Query: 614 ISPWLL 631 ISPWLL Sbjct: 1532 ISPWLL 1537 Score = 113 bits (273), Expect = 1e-25 Identities = 55/84 (65%), Positives = 65/84 (77%) Frame = +3 Query: 3 DLSPIKVIKGVSDLLKKCVIVAGDDRLSKQANENATLLFQCLVRSTLCTKFVSEEHRLSS 182 DL P++VI+GV +L KK VIVAG+D +SKQA NATLLF L+RSTLCTK V+EE LSS Sbjct: 1328 DLHPLRVIEGVRELSKKLVIVAGEDHISKQAQFNATLLFNILLRSTLCTKRVAEEFHLSS 1387 Query: 183 VAFEWLIGEIEARFQQAQVNPGEM 254 AFEWL+GEIE RF + PGEM Sbjct: 1388 EAFEWLLGEIETRFNHSHGKPGEM 1411 Score = 42.7 bits (96), Expect = 3e-04 Identities = 21/29 (72%), Positives = 24/29 (82%), Gaps = 2/29 (6%) Frame = +3 Query: 618 RLGYW--RIELDRKRMTDKKLTWEQIAEE 698 R+ W RIELDRKRMTDKKLT EQI+E+ Sbjct: 1531 RISPWLLRIELDRKRMTDKKLTMEQISEK 1559 >SB_19734| Best HMM Match : RNA_pol_Rpb1_5 (HMM E-Value=0) Length = 1452 Score = 75.8 bits (178), Expect = 4e-14 Identities = 40/81 (49%), Positives = 54/81 (66%), Gaps = 1/81 (1%) Frame = +2 Query: 254 VGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEII-NISKKPKAPSLTVFLTG 430 VG LAAQS+GEP+TQMTLNTFHFAG NVTLG+PR++EI+ S K P++ + L Sbjct: 592 VGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRMREILMTASANIKTPTMDMPLLA 651 Query: 431 GAARDAEKAKNVLCRLEHTTL 493 G + ++AK + +L L Sbjct: 652 G-KKVIKRAKKLQQKLNRVML 671 >SB_45318| Best HMM Match : RNA_pol_Rpb1_7 (HMM E-Value=0) Length = 116 Score = 71.7 bits (168), Expect = 6e-13 Identities = 30/31 (96%), Positives = 31/31 (100%) Frame = +1 Query: 511 TAIYYDPDPQNTVIAEDQEFVNVYYEMPDFD 603 TAIYYDPDPQNTVIAEDQ+FVNVYYEMPDFD Sbjct: 18 TAIYYDPDPQNTVIAEDQDFVNVYYEMPDFD 48 Score = 42.7 bits (96), Expect = 3e-04 Identities = 21/29 (72%), Positives = 24/29 (82%), Gaps = 2/29 (6%) Frame = +3 Query: 618 RLGYW--RIELDRKRMTDKKLTWEQIAEE 698 R+ W RIELDRKRMTDKKLT EQI+E+ Sbjct: 51 RISPWLLRIELDRKRMTDKKLTMEQISEK 79 Score = 38.3 bits (85), Expect = 0.007 Identities = 22/56 (39%), Positives = 27/56 (48%) Frame = +2 Query: 464 VLCRLEHTTLRKVTAXXXXXXXXXXXXXXXXXXKNLSMFTTKCLTLILAKISPWLL 631 VLCRLEHTTLRKVTA ++ + +A+ISPWLL Sbjct: 2 VLCRLEHTTLRKVTANTAIYYDPDPQNTVIAEDQDFVNVYYEMPDFDVARISPWLL 57 >SB_24568| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1109 Score = 58.4 bits (135), Expect = 6e-09 Identities = 39/88 (44%), Positives = 50/88 (56%), Gaps = 4/88 (4%) Frame = +2 Query: 254 VGALAAQSLGEPATQMTLNTFHFAGVSSK----NVTLGVPRLKEIINISKKPKAPSLTVF 421 VGA+ AQS+GEPA T H K ++TLGVPR+KEIIN S+K P ++ Sbjct: 975 VGAICAQSIGEPALP-TYEDVHVGIEEHKLLHVDITLGVPRIKEIINASRKISTPIISAQ 1033 Query: 422 LTGGAARDAEKAKNVLCRLEHTTLRKVT 505 L RD E A+ V R+E T L +VT Sbjct: 1034 LQDD--RDQELARAVKGRIERTLLGEVT 1059 >SB_7831| Best HMM Match : RNA_pol_Rpb1_7 (HMM E-Value=0) Length = 1467 Score = 53.2 bits (122), Expect = 2e-07 Identities = 22/23 (95%), Positives = 23/23 (100%) Frame = +1 Query: 535 PQNTVIAEDQEFVNVYYEMPDFD 603 PQNTVIAEDQ+FVNVYYEMPDFD Sbjct: 2 PQNTVIAEDQDFVNVYYEMPDFD 24 Score = 42.7 bits (96), Expect = 3e-04 Identities = 21/29 (72%), Positives = 24/29 (82%), Gaps = 2/29 (6%) Frame = +3 Query: 618 RLGYW--RIELDRKRMTDKKLTWEQIAEE 698 R+ W RIELDRKRMTDKKLT EQI+E+ Sbjct: 27 RISPWLLRIELDRKRMTDKKLTMEQISEK 55 >SB_21907| Best HMM Match : RNA_pol_Rpb1_5 (HMM E-Value=1.3e-21) Length = 380 Score = 44.4 bits (100), Expect = 1e-04 Identities = 18/31 (58%), Positives = 24/31 (77%) Frame = +2 Query: 251 NVGALAAQSLGEPATQMTLNTFHFAGVSSKN 343 +VG +AAQS+GEP TQ+TL TFH GV+ + Sbjct: 133 SVGIIAAQSIGEPGTQLTLRTFHVGGVAGSS 163 >SB_33304| Best HMM Match : RNA_pol_Rpb1_1 (HMM E-Value=7.00649e-45) Length = 638 Score = 44.0 bits (99), Expect = 1e-04 Identities = 18/27 (66%), Positives = 22/27 (81%) Frame = +2 Query: 254 VGALAAQSLGEPATQMTLNTFHFAGVS 334 VG +AAQS+GEP TQ+TL TFH GV+ Sbjct: 515 VGVIAAQSIGEPGTQLTLRTFHVGGVA 541 >SB_47320| Best HMM Match : Ion_trans (HMM E-Value=2.7e-34) Length = 946 Score = 30.7 bits (66), Expect = 1.3 Identities = 14/37 (37%), Positives = 25/37 (67%) Frame = -3 Query: 516 SRVAVTLRKVVCSNLQRTFLAFSASLAAPPVKNTVRE 406 S +AVT+ + S+ ++TF+ SAS++APP N + + Sbjct: 816 SDLAVTMGGQLTSSSEKTFVKSSASISAPPSLNDISD 852 >SB_57150| Best HMM Match : ThiF (HMM E-Value=2.8e-35) Length = 1026 Score = 30.3 bits (65), Expect = 1.8 Identities = 19/53 (35%), Positives = 26/53 (49%) Frame = -2 Query: 196 HSNATLDNLCSSDTNFVQSVDLTKHWKSNVAFSLACLDKRSSPATMTHFFRRS 38 H A + LC +D N D+ W+ N F L +K SSP T+F RR+ Sbjct: 873 HVKALVLELCCNDEN---DEDVEVPWRPNT-FRLLQHNKNSSPLDNTYFIRRT 921 >SB_26367| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 319 Score = 29.1 bits (62), Expect = 4.1 Identities = 21/70 (30%), Positives = 32/70 (45%), Gaps = 2/70 (2%) Frame = -3 Query: 678 MLISCRSFSYGPVQYANNQGEIFARIKVRHFVVNIDKFL--VLCNNSILRIRVIINSRVA 505 ML S R F + +QG + + V HF++NI + V+ + L I +V Sbjct: 78 MLYSARMFMRFYSKAQGDQGLFWTQKMVNHFMINISVLIVTVMSIDRFLSIYKPFKYKVI 137 Query: 504 VTLRKVVCSN 475 VT R+V N Sbjct: 138 VTSRRVATCN 147 >SB_17864| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 911 Score = 28.7 bits (61), Expect = 5.4 Identities = 20/55 (36%), Positives = 28/55 (50%), Gaps = 5/55 (9%) Frame = +2 Query: 239 KSR*NVGALAA-----QSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEIINIS 388 +SR N LAA + G A + LNT A V S+ T G+P +K +NI+ Sbjct: 809 RSRLNTTGLAAVRSHLNTTGLAAVRFRLNTTGLAAVRSRLNTTGLPAVKSRLNIT 863 >SB_4456| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 673 Score = 28.3 bits (60), Expect = 7.1 Identities = 12/41 (29%), Positives = 25/41 (60%), Gaps = 1/41 (2%) Frame = +1 Query: 247 VKCRRFS-CSITW*ASYSDDIEYLPFCWCIIQKCNTWCASS 366 +K ++S C+ T A++ ++ + F WC QKC+ + A++ Sbjct: 349 IKLIKYSACASTEPATFPTQLQPMGFLWCKQQKCHCYMAAT 389 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 22,796,690 Number of Sequences: 59808 Number of extensions: 449349 Number of successful extensions: 1041 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 940 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1035 length of database: 16,821,457 effective HSP length: 80 effective length of database: 12,036,817 effective search space used: 2046258890 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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