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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= tesV0355.Seq
         (752 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_44922| Best HMM Match : RNA_pol_Rpb1_1 (HMM E-Value=0)             161   6e-40
SB_19734| Best HMM Match : RNA_pol_Rpb1_5 (HMM E-Value=0)              76   4e-14
SB_45318| Best HMM Match : RNA_pol_Rpb1_7 (HMM E-Value=0)              72   6e-13
SB_24568| Best HMM Match : No HMM Matches (HMM E-Value=.)              58   6e-09
SB_7831| Best HMM Match : RNA_pol_Rpb1_7 (HMM E-Value=0)               53   2e-07
SB_21907| Best HMM Match : RNA_pol_Rpb1_5 (HMM E-Value=1.3e-21)        44   1e-04
SB_33304| Best HMM Match : RNA_pol_Rpb1_1 (HMM E-Value=7.00649e-45)    44   1e-04
SB_47320| Best HMM Match : Ion_trans (HMM E-Value=2.7e-34)             31   1.3  
SB_57150| Best HMM Match : ThiF (HMM E-Value=2.8e-35)                  30   1.8  
SB_26367| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   4.1  
SB_17864| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   5.4  
SB_4456| Best HMM Match : No HMM Matches (HMM E-Value=.)               28   7.1  

>SB_44922| Best HMM Match : RNA_pol_Rpb1_1 (HMM E-Value=0)
          Length = 1596

 Score =  161 bits (391), Expect = 6e-40
 Identities = 84/126 (66%), Positives = 92/126 (73%)
 Frame = +2

Query: 254  VGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEIINISKKPKAPSLTVFLTGG 433
            VGALAAQSLGEPATQMTLNTFH+AGVS+KNVTLGVPRLKEIIN+SKKPK PSLTVFL G 
Sbjct: 1412 VGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKEIINVSKKPKTPSLTVFLIGQ 1471

Query: 434  AARDAEKAKNVLCRLEHTTLRKVTAXXXXXXXXXXXXXXXXXXKNLSMFTTKCLTLILAK 613
            AARDAE AK VLCRLEHTTLRKVTA                  ++      +     +A+
Sbjct: 1472 AARDAEMAKAVLCRLEHTTLRKVTANTAIYYDPDPQNTVIAEDQDFVNVYYEMPDFDVAR 1531

Query: 614  ISPWLL 631
            ISPWLL
Sbjct: 1532 ISPWLL 1537



 Score =  113 bits (273), Expect = 1e-25
 Identities = 55/84 (65%), Positives = 65/84 (77%)
 Frame = +3

Query: 3    DLSPIKVIKGVSDLLKKCVIVAGDDRLSKQANENATLLFQCLVRSTLCTKFVSEEHRLSS 182
            DL P++VI+GV +L KK VIVAG+D +SKQA  NATLLF  L+RSTLCTK V+EE  LSS
Sbjct: 1328 DLHPLRVIEGVRELSKKLVIVAGEDHISKQAQFNATLLFNILLRSTLCTKRVAEEFHLSS 1387

Query: 183  VAFEWLIGEIEARFQQAQVNPGEM 254
             AFEWL+GEIE RF  +   PGEM
Sbjct: 1388 EAFEWLLGEIETRFNHSHGKPGEM 1411



 Score = 42.7 bits (96), Expect = 3e-04
 Identities = 21/29 (72%), Positives = 24/29 (82%), Gaps = 2/29 (6%)
 Frame = +3

Query: 618  RLGYW--RIELDRKRMTDKKLTWEQIAEE 698
            R+  W  RIELDRKRMTDKKLT EQI+E+
Sbjct: 1531 RISPWLLRIELDRKRMTDKKLTMEQISEK 1559


>SB_19734| Best HMM Match : RNA_pol_Rpb1_5 (HMM E-Value=0)
          Length = 1452

 Score = 75.8 bits (178), Expect = 4e-14
 Identities = 40/81 (49%), Positives = 54/81 (66%), Gaps = 1/81 (1%)
 Frame = +2

Query: 254 VGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEII-NISKKPKAPSLTVFLTG 430
           VG LAAQS+GEP+TQMTLNTFHFAG    NVTLG+PR++EI+   S   K P++ + L  
Sbjct: 592 VGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRMREILMTASANIKTPTMDMPLLA 651

Query: 431 GAARDAEKAKNVLCRLEHTTL 493
           G  +  ++AK +  +L    L
Sbjct: 652 G-KKVIKRAKKLQQKLNRVML 671


>SB_45318| Best HMM Match : RNA_pol_Rpb1_7 (HMM E-Value=0)
          Length = 116

 Score = 71.7 bits (168), Expect = 6e-13
 Identities = 30/31 (96%), Positives = 31/31 (100%)
 Frame = +1

Query: 511 TAIYYDPDPQNTVIAEDQEFVNVYYEMPDFD 603
           TAIYYDPDPQNTVIAEDQ+FVNVYYEMPDFD
Sbjct: 18  TAIYYDPDPQNTVIAEDQDFVNVYYEMPDFD 48



 Score = 42.7 bits (96), Expect = 3e-04
 Identities = 21/29 (72%), Positives = 24/29 (82%), Gaps = 2/29 (6%)
 Frame = +3

Query: 618 RLGYW--RIELDRKRMTDKKLTWEQIAEE 698
           R+  W  RIELDRKRMTDKKLT EQI+E+
Sbjct: 51  RISPWLLRIELDRKRMTDKKLTMEQISEK 79



 Score = 38.3 bits (85), Expect = 0.007
 Identities = 22/56 (39%), Positives = 27/56 (48%)
 Frame = +2

Query: 464 VLCRLEHTTLRKVTAXXXXXXXXXXXXXXXXXXKNLSMFTTKCLTLILAKISPWLL 631
           VLCRLEHTTLRKVTA                  ++      +     +A+ISPWLL
Sbjct: 2   VLCRLEHTTLRKVTANTAIYYDPDPQNTVIAEDQDFVNVYYEMPDFDVARISPWLL 57


>SB_24568| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1109

 Score = 58.4 bits (135), Expect = 6e-09
 Identities = 39/88 (44%), Positives = 50/88 (56%), Gaps = 4/88 (4%)
 Frame = +2

Query: 254  VGALAAQSLGEPATQMTLNTFHFAGVSSK----NVTLGVPRLKEIINISKKPKAPSLTVF 421
            VGA+ AQS+GEPA   T    H      K    ++TLGVPR+KEIIN S+K   P ++  
Sbjct: 975  VGAICAQSIGEPALP-TYEDVHVGIEEHKLLHVDITLGVPRIKEIINASRKISTPIISAQ 1033

Query: 422  LTGGAARDAEKAKNVLCRLEHTTLRKVT 505
            L     RD E A+ V  R+E T L +VT
Sbjct: 1034 LQDD--RDQELARAVKGRIERTLLGEVT 1059


>SB_7831| Best HMM Match : RNA_pol_Rpb1_7 (HMM E-Value=0)
          Length = 1467

 Score = 53.2 bits (122), Expect = 2e-07
 Identities = 22/23 (95%), Positives = 23/23 (100%)
 Frame = +1

Query: 535 PQNTVIAEDQEFVNVYYEMPDFD 603
           PQNTVIAEDQ+FVNVYYEMPDFD
Sbjct: 2   PQNTVIAEDQDFVNVYYEMPDFD 24



 Score = 42.7 bits (96), Expect = 3e-04
 Identities = 21/29 (72%), Positives = 24/29 (82%), Gaps = 2/29 (6%)
 Frame = +3

Query: 618 RLGYW--RIELDRKRMTDKKLTWEQIAEE 698
           R+  W  RIELDRKRMTDKKLT EQI+E+
Sbjct: 27  RISPWLLRIELDRKRMTDKKLTMEQISEK 55


>SB_21907| Best HMM Match : RNA_pol_Rpb1_5 (HMM E-Value=1.3e-21)
          Length = 380

 Score = 44.4 bits (100), Expect = 1e-04
 Identities = 18/31 (58%), Positives = 24/31 (77%)
 Frame = +2

Query: 251 NVGALAAQSLGEPATQMTLNTFHFAGVSSKN 343
           +VG +AAQS+GEP TQ+TL TFH  GV+  +
Sbjct: 133 SVGIIAAQSIGEPGTQLTLRTFHVGGVAGSS 163


>SB_33304| Best HMM Match : RNA_pol_Rpb1_1 (HMM E-Value=7.00649e-45)
          Length = 638

 Score = 44.0 bits (99), Expect = 1e-04
 Identities = 18/27 (66%), Positives = 22/27 (81%)
 Frame = +2

Query: 254 VGALAAQSLGEPATQMTLNTFHFAGVS 334
           VG +AAQS+GEP TQ+TL TFH  GV+
Sbjct: 515 VGVIAAQSIGEPGTQLTLRTFHVGGVA 541


>SB_47320| Best HMM Match : Ion_trans (HMM E-Value=2.7e-34)
          Length = 946

 Score = 30.7 bits (66), Expect = 1.3
 Identities = 14/37 (37%), Positives = 25/37 (67%)
 Frame = -3

Query: 516 SRVAVTLRKVVCSNLQRTFLAFSASLAAPPVKNTVRE 406
           S +AVT+   + S+ ++TF+  SAS++APP  N + +
Sbjct: 816 SDLAVTMGGQLTSSSEKTFVKSSASISAPPSLNDISD 852


>SB_57150| Best HMM Match : ThiF (HMM E-Value=2.8e-35)
          Length = 1026

 Score = 30.3 bits (65), Expect = 1.8
 Identities = 19/53 (35%), Positives = 26/53 (49%)
 Frame = -2

Query: 196  HSNATLDNLCSSDTNFVQSVDLTKHWKSNVAFSLACLDKRSSPATMTHFFRRS 38
            H  A +  LC +D N     D+   W+ N  F L   +K SSP   T+F RR+
Sbjct: 873  HVKALVLELCCNDEN---DEDVEVPWRPNT-FRLLQHNKNSSPLDNTYFIRRT 921


>SB_26367| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 319

 Score = 29.1 bits (62), Expect = 4.1
 Identities = 21/70 (30%), Positives = 32/70 (45%), Gaps = 2/70 (2%)
 Frame = -3

Query: 678 MLISCRSFSYGPVQYANNQGEIFARIKVRHFVVNIDKFL--VLCNNSILRIRVIINSRVA 505
           ML S R F     +   +QG  + +  V HF++NI   +  V+  +  L I      +V 
Sbjct: 78  MLYSARMFMRFYSKAQGDQGLFWTQKMVNHFMINISVLIVTVMSIDRFLSIYKPFKYKVI 137

Query: 504 VTLRKVVCSN 475
           VT R+V   N
Sbjct: 138 VTSRRVATCN 147


>SB_17864| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 911

 Score = 28.7 bits (61), Expect = 5.4
 Identities = 20/55 (36%), Positives = 28/55 (50%), Gaps = 5/55 (9%)
 Frame = +2

Query: 239 KSR*NVGALAA-----QSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEIINIS 388
           +SR N   LAA      + G  A +  LNT   A V S+  T G+P +K  +NI+
Sbjct: 809 RSRLNTTGLAAVRSHLNTTGLAAVRFRLNTTGLAAVRSRLNTTGLPAVKSRLNIT 863


>SB_4456| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 673

 Score = 28.3 bits (60), Expect = 7.1
 Identities = 12/41 (29%), Positives = 25/41 (60%), Gaps = 1/41 (2%)
 Frame = +1

Query: 247 VKCRRFS-CSITW*ASYSDDIEYLPFCWCIIQKCNTWCASS 366
           +K  ++S C+ T  A++   ++ + F WC  QKC+ + A++
Sbjct: 349 IKLIKYSACASTEPATFPTQLQPMGFLWCKQQKCHCYMAAT 389


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,796,690
Number of Sequences: 59808
Number of extensions: 449349
Number of successful extensions: 1041
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 940
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1035
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 2046258890
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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