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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= tesV0355.Seq
         (752 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ...   125   4e-29
At5g60040.1 68418.m07529 DNA-directed RNA polymerase, putative s...    81   6e-16
At3g57660.1 68416.m06424 DNA-directed RNA polymerase family prot...    76   2e-14
At2g02550.1 68415.m00194 exonuclease family protein contains sim...    30   1.4  
At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein si...    30   1.9  
At3g05680.1 68416.m00634 expressed protein                             28   5.8  
At3g11760.1 68416.m01443 expressed protein                             28   7.7  
At2g07770.1 68415.m01005 hypothetical protein low similarity to ...    28   7.7  

>At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest
            subunit (RPB205) (RPII) (RPB1) nearly identical to
            P|P18616 DNA-directed RNA polymerase II largest subunit
            (EC 2.7.7.6) {Arabidopsis thaliana}
          Length = 1840

 Score =  125 bits (301), Expect = 4e-29
 Identities = 58/84 (69%), Positives = 72/84 (85%)
 Frame = +2

Query: 254  VGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEIINISKKPKAPSLTVFLTGG 433
            +G +AAQS+GEPATQMTLNTFH+AGVS+KNVTLGVPRL+EIIN++K+ K PSL+V+LT  
Sbjct: 1085 IGCVAAQSIGEPATQMTLNTFHYAGVSAKNVTLGVPRLREIINVAKRIKTPSLSVYLTPE 1144

Query: 434  AARDAEKAKNVLCRLEHTTLRKVT 505
            A++  E AK V C LE+TTLR VT
Sbjct: 1145 ASKSKEGAKTVQCALEYTTLRSVT 1168



 Score = 95.9 bits (228), Expect = 3e-20
 Identities = 44/84 (52%), Positives = 62/84 (73%)
 Frame = +3

Query: 3    DLSPIKVIKGVSDLLKKCVIVAGDDRLSKQANENATLLFQCLVRSTLCTKFVSEEHRLSS 182
            D+ P++++  V  L ++ ++V GDD LS +A +NATL F  L+RSTL +K V EE++LS 
Sbjct: 1001 DMHPVEIVDAVDKLQERLLVVPGDDALSVEAQKNATLFFNILLRSTLASKRVLEEYKLSR 1060

Query: 183  VAFEWLIGEIEARFQQAQVNPGEM 254
             AFEW+IGEIE+RF Q+ V PGEM
Sbjct: 1061 EAFEWVIGEIESRFLQSLVAPGEM 1084



 Score = 50.4 bits (115), Expect = 1e-06
 Identities = 20/31 (64%), Positives = 24/31 (77%)
 Frame = +1

Query: 511  TAIYYDPDPQNTVIAEDQEFVNVYYEMPDFD 603
            T ++YDPDP +T+I ED EFV  YYEMPD D
Sbjct: 1171 TEVWYDPDPMSTIIEEDFEFVRSYYEMPDED 1201



 Score = 29.1 bits (62), Expect = 3.3
 Identities = 13/22 (59%), Positives = 17/22 (77%)
 Frame = +3

Query: 633  RIELDRKRMTDKKLTWEQIAEE 698
            RIEL+R+ M DKKL+   IAE+
Sbjct: 1213 RIELNREMMVDKKLSMADIAEK 1234


>At5g60040.1 68418.m07529 DNA-directed RNA polymerase, putative
            similar to SP|P04051 DNA-directed RNA polymerase III
            largest subunit (EC 2.7.7.6) {Saccharomyces cerevisiae};
            contains InterPro accession IPR000722: RNA polymerase,
            alpha subunit
          Length = 1328

 Score = 81.4 bits (192), Expect = 6e-16
 Identities = 42/83 (50%), Positives = 54/83 (65%)
 Frame = +2

Query: 254  VGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEIINISKKPKAPSLTVFLTGG 433
            +G + AQS+GEP TQMTL TFHFAGV+S N+T GVPR+ EIIN SK    P ++  L   
Sbjct: 984  IGTIGAQSIGEPGTQMTLKTFHFAGVASMNITQGVPRINEIINASKNISTPVISAELEN- 1042

Query: 434  AARDAEKAKNVLCRLEHTTLRKV 502
               +   A+ V  R+E TTL +V
Sbjct: 1043 -PLELTSARWVKGRIEKTTLGQV 1064


>At3g57660.1 68416.m06424 DNA-directed RNA polymerase family protein
            similar to SP|O35134 DNA-directed RNA polymerase I
            largest subunit (EC 2.7.7.6) (RNA polymerase I 194 kDa
            subunit) (RPA194) {Mus musculus}; contains InterPro
            accession IPR000722: RNA polymerase, alpha subunit
          Length = 1670

 Score = 76.2 bits (179), Expect = 2e-14
 Identities = 44/98 (44%), Positives = 59/98 (60%), Gaps = 1/98 (1%)
 Frame = +2

Query: 212  RGKIPASTSKSR*NVGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEII-NIS 388
            + K  AS ++    VG LAAQS+GEP+TQMTLNTFH AG    NVTLG+PRL+EI+   +
Sbjct: 1144 KSKFFASLAQPGEPVGVLAAQSVGEPSTQMTLNTFHLAGRGEMNVTLGIPRLQEILMTAA 1203

Query: 389  KKPKAPSLTVFLTGGAARDAEKAKNVLCRLEHTTLRKV 502
               K P +T  L  G  +  E A ++  RL   T+  +
Sbjct: 1204 ANIKTPIMTCPLLKGKTK--EDANDITDRLRKITVADI 1239


>At2g02550.1 68415.m00194 exonuclease family protein contains
           similarity to exonuclease I [Homo sapiens]
           gi|4249655|gb|AAD13754
          Length = 200

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 15/44 (34%), Positives = 23/44 (52%)
 Frame = +3

Query: 9   SPIKVIKGVSDLLKKCVIVAGDDRLSKQANENATLLFQCLVRST 140
           +PIK ++   DL     ++A DD   K   +NAT +FQ  +  T
Sbjct: 93  NPIKTLEYAKDLDASAQLMAPDDPKRKDKEKNATKIFQSHIHIT 136


>At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein
           similar to GTPase activating protein [Yarrowia
           lipolytica] GI:2370595; contains Pfam profile PF00566:
           TBC domain
          Length = 720

 Score = 29.9 bits (64), Expect = 1.9
 Identities = 16/42 (38%), Positives = 23/42 (54%)
 Frame = -3

Query: 615 IFARIKVRHFVVNIDKFLVLCNNSILRIRVIINSRVAVTLRK 490
           I  R K+     +ID  + +CN+ +L IRV    R A+ LRK
Sbjct: 617 IMNRHKIMKETRSIDDMIKICNDKLLAIRVRRCIRTAIKLRK 658


>At3g05680.1 68416.m00634 expressed protein
          Length = 2057

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 13/65 (20%), Positives = 31/65 (47%)
 Frame = -3

Query: 597  VRHFVVNIDKFLVLCNNSILRIRVIINSRVAVTLRKVVCSNLQRTFLAFSASLAAPPVKN 418
            + H   N+DK   +  +  +   V+I   + V + ++ C  ++       AS+   P+ +
Sbjct: 1009 INHLTPNLDKIAKIIQHHAVSALVVIQDMLRVFIVRIACQRVEH------ASILLRPIFS 1062

Query: 417  TVREG 403
            ++R+G
Sbjct: 1063 SIRDG 1067


>At3g11760.1 68416.m01443 expressed protein 
          Length = 702

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 11/28 (39%), Positives = 17/28 (60%)
 Frame = -3

Query: 702 NFPQQSVPMLISCRSFSYGPVQYANNQG 619
           +F +    ++   +SFSYGP+ YAN  G
Sbjct: 260 DFDEGKEELMSMRKSFSYGPLSYANGVG 287


>At2g07770.1 68415.m01005 hypothetical protein low similarity to KED
           [Nicotiana tabacum] GI:8096269; contains Pfam profile
           PF03384: Drosophila protein of unknown function, DUF287
          Length = 518

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 20/87 (22%), Positives = 42/87 (48%)
 Frame = -3

Query: 729 EIITKTQPLNFPQQSVPMLISCRSFSYGPVQYANNQGEIFARIKVRHFVVNIDKFLVLCN 550
           E++  +    F  ++V  L  C++F +G   +  N  +IF  IK  + VV   K   +  
Sbjct: 24  EVVVASAAEKFFLRAVADLELCKTFPWGRFAFEENVKDIFYLIKKCNEVVGPQK---VFP 80

Query: 549 NSILRIRVIINSRVAVTLRKVVCSNLQ 469
           + ++ +  +    + V LRK+ C +++
Sbjct: 81  SFVMPLEYLAFEAIPV-LRKIFCEDIE 106


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,074,975
Number of Sequences: 28952
Number of extensions: 324544
Number of successful extensions: 780
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 767
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 780
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1672953192
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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