BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesV0355.Seq (752 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ... 125 4e-29 At5g60040.1 68418.m07529 DNA-directed RNA polymerase, putative s... 81 6e-16 At3g57660.1 68416.m06424 DNA-directed RNA polymerase family prot... 76 2e-14 At2g02550.1 68415.m00194 exonuclease family protein contains sim... 30 1.4 At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein si... 30 1.9 At3g05680.1 68416.m00634 expressed protein 28 5.8 At3g11760.1 68416.m01443 expressed protein 28 7.7 At2g07770.1 68415.m01005 hypothetical protein low similarity to ... 28 7.7 >At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest subunit (RPB205) (RPII) (RPB1) nearly identical to P|P18616 DNA-directed RNA polymerase II largest subunit (EC 2.7.7.6) {Arabidopsis thaliana} Length = 1840 Score = 125 bits (301), Expect = 4e-29 Identities = 58/84 (69%), Positives = 72/84 (85%) Frame = +2 Query: 254 VGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEIINISKKPKAPSLTVFLTGG 433 +G +AAQS+GEPATQMTLNTFH+AGVS+KNVTLGVPRL+EIIN++K+ K PSL+V+LT Sbjct: 1085 IGCVAAQSIGEPATQMTLNTFHYAGVSAKNVTLGVPRLREIINVAKRIKTPSLSVYLTPE 1144 Query: 434 AARDAEKAKNVLCRLEHTTLRKVT 505 A++ E AK V C LE+TTLR VT Sbjct: 1145 ASKSKEGAKTVQCALEYTTLRSVT 1168 Score = 95.9 bits (228), Expect = 3e-20 Identities = 44/84 (52%), Positives = 62/84 (73%) Frame = +3 Query: 3 DLSPIKVIKGVSDLLKKCVIVAGDDRLSKQANENATLLFQCLVRSTLCTKFVSEEHRLSS 182 D+ P++++ V L ++ ++V GDD LS +A +NATL F L+RSTL +K V EE++LS Sbjct: 1001 DMHPVEIVDAVDKLQERLLVVPGDDALSVEAQKNATLFFNILLRSTLASKRVLEEYKLSR 1060 Query: 183 VAFEWLIGEIEARFQQAQVNPGEM 254 AFEW+IGEIE+RF Q+ V PGEM Sbjct: 1061 EAFEWVIGEIESRFLQSLVAPGEM 1084 Score = 50.4 bits (115), Expect = 1e-06 Identities = 20/31 (64%), Positives = 24/31 (77%) Frame = +1 Query: 511 TAIYYDPDPQNTVIAEDQEFVNVYYEMPDFD 603 T ++YDPDP +T+I ED EFV YYEMPD D Sbjct: 1171 TEVWYDPDPMSTIIEEDFEFVRSYYEMPDED 1201 Score = 29.1 bits (62), Expect = 3.3 Identities = 13/22 (59%), Positives = 17/22 (77%) Frame = +3 Query: 633 RIELDRKRMTDKKLTWEQIAEE 698 RIEL+R+ M DKKL+ IAE+ Sbjct: 1213 RIELNREMMVDKKLSMADIAEK 1234 >At5g60040.1 68418.m07529 DNA-directed RNA polymerase, putative similar to SP|P04051 DNA-directed RNA polymerase III largest subunit (EC 2.7.7.6) {Saccharomyces cerevisiae}; contains InterPro accession IPR000722: RNA polymerase, alpha subunit Length = 1328 Score = 81.4 bits (192), Expect = 6e-16 Identities = 42/83 (50%), Positives = 54/83 (65%) Frame = +2 Query: 254 VGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEIINISKKPKAPSLTVFLTGG 433 +G + AQS+GEP TQMTL TFHFAGV+S N+T GVPR+ EIIN SK P ++ L Sbjct: 984 IGTIGAQSIGEPGTQMTLKTFHFAGVASMNITQGVPRINEIINASKNISTPVISAELEN- 1042 Query: 434 AARDAEKAKNVLCRLEHTTLRKV 502 + A+ V R+E TTL +V Sbjct: 1043 -PLELTSARWVKGRIEKTTLGQV 1064 >At3g57660.1 68416.m06424 DNA-directed RNA polymerase family protein similar to SP|O35134 DNA-directed RNA polymerase I largest subunit (EC 2.7.7.6) (RNA polymerase I 194 kDa subunit) (RPA194) {Mus musculus}; contains InterPro accession IPR000722: RNA polymerase, alpha subunit Length = 1670 Score = 76.2 bits (179), Expect = 2e-14 Identities = 44/98 (44%), Positives = 59/98 (60%), Gaps = 1/98 (1%) Frame = +2 Query: 212 RGKIPASTSKSR*NVGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEII-NIS 388 + K AS ++ VG LAAQS+GEP+TQMTLNTFH AG NVTLG+PRL+EI+ + Sbjct: 1144 KSKFFASLAQPGEPVGVLAAQSVGEPSTQMTLNTFHLAGRGEMNVTLGIPRLQEILMTAA 1203 Query: 389 KKPKAPSLTVFLTGGAARDAEKAKNVLCRLEHTTLRKV 502 K P +T L G + E A ++ RL T+ + Sbjct: 1204 ANIKTPIMTCPLLKGKTK--EDANDITDRLRKITVADI 1239 >At2g02550.1 68415.m00194 exonuclease family protein contains similarity to exonuclease I [Homo sapiens] gi|4249655|gb|AAD13754 Length = 200 Score = 30.3 bits (65), Expect = 1.4 Identities = 15/44 (34%), Positives = 23/44 (52%) Frame = +3 Query: 9 SPIKVIKGVSDLLKKCVIVAGDDRLSKQANENATLLFQCLVRST 140 +PIK ++ DL ++A DD K +NAT +FQ + T Sbjct: 93 NPIKTLEYAKDLDASAQLMAPDDPKRKDKEKNATKIFQSHIHIT 136 >At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein similar to GTPase activating protein [Yarrowia lipolytica] GI:2370595; contains Pfam profile PF00566: TBC domain Length = 720 Score = 29.9 bits (64), Expect = 1.9 Identities = 16/42 (38%), Positives = 23/42 (54%) Frame = -3 Query: 615 IFARIKVRHFVVNIDKFLVLCNNSILRIRVIINSRVAVTLRK 490 I R K+ +ID + +CN+ +L IRV R A+ LRK Sbjct: 617 IMNRHKIMKETRSIDDMIKICNDKLLAIRVRRCIRTAIKLRK 658 >At3g05680.1 68416.m00634 expressed protein Length = 2057 Score = 28.3 bits (60), Expect = 5.8 Identities = 13/65 (20%), Positives = 31/65 (47%) Frame = -3 Query: 597 VRHFVVNIDKFLVLCNNSILRIRVIINSRVAVTLRKVVCSNLQRTFLAFSASLAAPPVKN 418 + H N+DK + + + V+I + V + ++ C ++ AS+ P+ + Sbjct: 1009 INHLTPNLDKIAKIIQHHAVSALVVIQDMLRVFIVRIACQRVEH------ASILLRPIFS 1062 Query: 417 TVREG 403 ++R+G Sbjct: 1063 SIRDG 1067 >At3g11760.1 68416.m01443 expressed protein Length = 702 Score = 27.9 bits (59), Expect = 7.7 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = -3 Query: 702 NFPQQSVPMLISCRSFSYGPVQYANNQG 619 +F + ++ +SFSYGP+ YAN G Sbjct: 260 DFDEGKEELMSMRKSFSYGPLSYANGVG 287 >At2g07770.1 68415.m01005 hypothetical protein low similarity to KED [Nicotiana tabacum] GI:8096269; contains Pfam profile PF03384: Drosophila protein of unknown function, DUF287 Length = 518 Score = 27.9 bits (59), Expect = 7.7 Identities = 20/87 (22%), Positives = 42/87 (48%) Frame = -3 Query: 729 EIITKTQPLNFPQQSVPMLISCRSFSYGPVQYANNQGEIFARIKVRHFVVNIDKFLVLCN 550 E++ + F ++V L C++F +G + N +IF IK + VV K + Sbjct: 24 EVVVASAAEKFFLRAVADLELCKTFPWGRFAFEENVKDIFYLIKKCNEVVGPQK---VFP 80 Query: 549 NSILRIRVIINSRVAVTLRKVVCSNLQ 469 + ++ + + + V LRK+ C +++ Sbjct: 81 SFVMPLEYLAFEAIPV-LRKIFCEDIE 106 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,074,975 Number of Sequences: 28952 Number of extensions: 324544 Number of successful extensions: 780 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 767 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 780 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1672953192 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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