BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesV0342.Seq (698 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC24H6.03 |cul3|pcu3|cullin 3|Schizosaccharomyces pombe|chr 1|... 70 3e-13 SPAC17G6.12 |cul1|pcu1|cullin 1|Schizosaccharomyces pombe|chr 1|... 60 4e-10 SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces pombe... 57 2e-09 SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces pombe... 27 2.6 SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo... 27 2.6 SPAC15E1.04 |||thymidylate synthase |Schizosaccharomyces pombe|c... 27 3.4 SPAC19G12.02c |pms1||MutL family mismatch-repair protein Pms1|Sc... 27 3.4 SPCC162.07 |ent1||epsin|Schizosaccharomyces pombe|chr 3|||Manual 27 3.4 SPAC24H6.09 |gef1||RhoGEF Gef1|Schizosaccharomyces pombe|chr 1||... 26 6.0 SPBC13G1.13 |tfb2|SPBC31F10.01|transcription factor TFIIH comple... 25 7.9 >SPAC24H6.03 |cul3|pcu3|cullin 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 785 Score = 70.1 bits (164), Expect = 3e-13 Identities = 32/83 (38%), Positives = 51/83 (61%) Frame = +2 Query: 2 RFDHFLQNSFNNDKIFKHMITSDFEYFLNLNNKSPEFLSLFIDGKLKKGEKGMSEQEIEA 181 R + + + + D+ + ++ F F++ ++PE++SLFID LKK + E IEA Sbjct: 388 RLNTIISTTMDADRSILNSLSDAFVTFVDGYTRAPEYISLFIDDNLKKDARKAIEGSIEA 447 Query: 182 VLDKTMVLFRFLQEKDVFERYYK 250 L ++ LFRF+ EKDVFE+YYK Sbjct: 448 TLQNSVTLFRFISEKDVFEKYYK 470 Score = 67.7 bits (158), Expect = 1e-12 Identities = 32/72 (44%), Positives = 45/72 (62%) Frame = +1 Query: 277 NKSVSDDSEKNMISKLKTECGCQFTSKLEGMFKDMTVSNTIMDEFKEHVLSSGLNLYGVD 456 N+S+S D+E MIS+LK E G FT KLEGMF DM +S ++ E+K + +D Sbjct: 480 NRSISSDAELGMISRLKQEAGNVFTQKLEGMFNDMNLSQELLQEYKHNSALQSAK-PALD 538 Query: 457 LSVRVLTTGFWP 492 L+V +L + FWP Sbjct: 539 LNVSILASTFWP 550 Score = 36.3 bits (80), Expect = 0.004 Identities = 16/39 (41%), Positives = 21/39 (53%) Frame = +3 Query: 510 KCNIPAAPRXAFEVFRSFYLANTPGRQLSLQPQLGSADL 626 KCN P + F FYL+ GR+L P +GSAD+ Sbjct: 559 KCNFPKVLLAQIDQFTDFYLSKHTGRKLLWYPSMGSADV 597 >SPAC17G6.12 |cul1|pcu1|cullin 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 767 Score = 59.7 bits (138), Expect = 4e-10 Identities = 31/70 (44%), Positives = 44/70 (62%) Frame = +1 Query: 283 SVSDDSEKNMISKLKTECGCQFTSKLEGMFKDMTVSNTIMDEFKEHVLSSGLNLYGVDLS 462 S S D+E +M+SKLK CG ++TSKL+ MF+D+++S I + F + S N +D S Sbjct: 474 SNSQDAESSMLSKLKEVCGFEYTSKLQRMFQDISLSQEITEAFWQLPQSRAGN---IDFS 530 Query: 463 VRVLTTGFWP 492 VL T FWP Sbjct: 531 ALVLGTSFWP 540 Score = 52.0 bits (119), Expect = 8e-08 Identities = 24/87 (27%), Positives = 48/87 (55%), Gaps = 7/87 (8%) Frame = +2 Query: 17 LQNSFNNDKIFKHMITSDFEYFLNLN-------NKSPEFLSLFIDGKLKKGEKGMSEQEI 175 + +F+ D F + + F +N N ++SPE L+ + D L+K K + ++ Sbjct: 378 VNTAFHGDTDFTKSLDTAFRELVNRNVVCQRSSSRSPELLAKYADSILRKSNKNVDIDDV 437 Query: 176 EAVLDKTMVLFRFLQEKDVFERYYKQL 256 E L +++FR++++KDVF+ +Y +L Sbjct: 438 EDCLSSIIIIFRYVEDKDVFQNFYTKL 464 >SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces pombe|chr 1|||Manual Length = 734 Score = 57.2 bits (132), Expect = 2e-09 Identities = 28/73 (38%), Positives = 40/73 (54%) Frame = +1 Query: 277 NKSVSDDSEKNMISKLKTECGCQFTSKLEGMFKDMTVSNTIMDEFKEHVLSSGLNLYGVD 456 NKS S +E ++ LK CG QFT LEGMF+D+ +S F+ + L+ D Sbjct: 438 NKSASAQNELMLLDMLKKTCGSQFTHSLEGMFRDVNISKEFTSSFRHSKAAHNLHR---D 494 Query: 457 LSVRVLTTGFWPT 495 L V VL+ +WP+ Sbjct: 495 LYVNVLSQAYWPS 507 Score = 46.8 bits (106), Expect = 3e-06 Identities = 28/91 (30%), Positives = 48/91 (52%), Gaps = 9/91 (9%) Frame = +2 Query: 5 FDHFLQ----NSFNNDKIFKHMITSDFEYFLN----LNNKSP-EFLSLFIDGKLKKGEKG 157 F FLQ SF +D+ + + FE F+N ++P ++ +ID L+ GE+ Sbjct: 338 FHKFLQVVVDESFLHDETLSYAMRKAFETFINGAKGSQREAPARLIAKYIDYLLRVGEQA 397 Query: 158 MSEQEIEAVLDKTMVLFRFLQEKDVFERYYK 250 + ++ V + + LFR++ KD+FE YYK Sbjct: 398 SGGKPLKEVFSEILDLFRYIASKDIFEAYYK 428 >SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 871 Score = 27.1 bits (57), Expect = 2.6 Identities = 13/27 (48%), Positives = 17/27 (62%) Frame = +1 Query: 454 DLSVRVLTTGFWPTQSATRSATYRPRP 534 D+SV VLTT PT S +++ R RP Sbjct: 811 DMSVPVLTTSRMPTISLSKAKELRTRP 837 >SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 1811 Score = 27.1 bits (57), Expect = 2.6 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = +1 Query: 301 EKNMISKLKTECGCQFTSKLEGMFKDM 381 E +MISK+ C QF K + FKD+ Sbjct: 1521 ENSMISKVGLSCFSQFVLKNKNQFKDV 1547 >SPAC15E1.04 |||thymidylate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 625 Score = 26.6 bits (56), Expect = 3.4 Identities = 13/38 (34%), Positives = 19/38 (50%) Frame = -1 Query: 257 RAVYNNVRKRPSLVKIGIVPLFYPAQLRFPVRSSPFRL 144 R + N + RP G +F P QLRF +R++ L Sbjct: 338 RYILENGQSRPDRTGTGTRSVFAPPQLRFSLRNNTLPL 375 >SPAC19G12.02c |pms1||MutL family mismatch-repair protein Pms1|Schizosaccharomyces pombe|chr 1|||Manual Length = 794 Score = 26.6 bits (56), Expect = 3.4 Identities = 17/61 (27%), Positives = 31/61 (50%) Frame = +3 Query: 453 GSVRPSAHDRLLADAERDAKCNIPAAPRXAFEVFRSFYLANTPGRQLSLQPQLGSADLHA 632 GS R S ++L R N+P R EVF+ + +A +P ++L+ G+ D++ Sbjct: 247 GSTRASNERQMLFINRR--LVNLPKIARVIQEVFKPYSMAQSPFFAINLRITNGTIDINV 304 Query: 633 T 635 + Sbjct: 305 S 305 >SPCC162.07 |ent1||epsin|Schizosaccharomyces pombe|chr 3|||Manual Length = 706 Score = 26.6 bits (56), Expect = 3.4 Identities = 15/39 (38%), Positives = 21/39 (53%) Frame = -3 Query: 552 RLRTRSWARPVCCTSRRALRRPEAGREHSDGQIHSVQVQ 436 R R+RS RP SRR RRP EH+ + + ++Q Sbjct: 199 RRRSRS-RRPGRSRSRRRSRRPSPSAEHNSAEENDPELQ 236 >SPAC24H6.09 |gef1||RhoGEF Gef1|Schizosaccharomyces pombe|chr 1|||Manual Length = 753 Score = 25.8 bits (54), Expect = 6.0 Identities = 23/84 (27%), Positives = 41/84 (48%), Gaps = 3/84 (3%) Frame = -1 Query: 455 STPYKFSPLESTC---SLNSSMMVLETVISLNIPSNFDVN*QPHSVLSLEIMFFSLSSET 285 ST SPL++T L+S+++ LET SL+ ++ P + LS E+ Sbjct: 240 STASLLSPLDTTSFSTKLDSTILALETDESLSRTISYATTSLPSTPGK------RLSKES 293 Query: 284 DLLSKSRFARAVYNNVRKRPSLVK 213 +S++ Y ++KR +L+K Sbjct: 294 LAMSEASSINPEYKRIKKRANLIK 317 >SPBC13G1.13 |tfb2|SPBC31F10.01|transcription factor TFIIH complex subunit Tfb2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 447 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/49 (26%), Positives = 26/49 (53%), Gaps = 1/49 (2%) Frame = +1 Query: 415 EHVLSSGLNLYGVDLSVRVLTTGFWPTQSATRSAT-YRPRPGXRSKSSE 558 + ++ L YG+ ++ + F+PT+ AT T YR G +S++ + Sbjct: 240 QQIMLEDLREYGLVYQRKITSKRFYPTRLATGLTTDYRSLHGKQSENDD 288 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,431,890 Number of Sequences: 5004 Number of extensions: 45354 Number of successful extensions: 144 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 136 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 144 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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