BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesV0336.Seq
(741 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g21400.1 68414.m02678 2-oxoisovalerate dehydrogenase, putativ... 145 2e-35
At5g09300.2 68418.m01077 2-oxoisovalerate dehydrogenase, putativ... 142 2e-34
At5g09300.1 68418.m01078 2-oxoisovalerate dehydrogenase, putativ... 142 2e-34
At1g04140.2 68414.m00404 transducin family protein / WD-40 repea... 33 0.26
At1g04140.1 68414.m00403 transducin family protein / WD-40 repea... 33 0.26
At5g35200.1 68418.m04171 epsin N-terminal homology (ENTH) domain... 28 5.7
At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alp... 28 5.7
At5g49660.1 68418.m06147 leucine-rich repeat transmembrane prote... 28 7.5
At5g24470.1 68418.m02884 pseudo-response regulator 5 (APRR5) ide... 28 7.5
At4g02590.1 68417.m00353 basic helix-loop-helix (bHLH) family pr... 28 7.5
>At1g21400.1 68414.m02678 2-oxoisovalerate dehydrogenase, putative /
3-methyl-2-oxobutanoate dehydrogenase, putative /
branched-chain alpha-keto acid dehydrogenase E1 alpha
subunit, putative similar to branched-chain alpha-keto
acid dehydrogenase E1-alpha subunit [Gallus gallus]
GI:12964598; contains Pfam profile PF00676:
Dehydrogenase E1 component
Length = 472
Score = 145 bits (352), Expect = 2e-35
Identities = 73/150 (48%), Positives = 92/150 (61%)
Frame = +3
Query: 255 GARAPYVSEMKFFNETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKTMVQLSHM 434
G + Y SEMKF E+S IP YRV+D +G+II ++ + + + MY+ M L M
Sbjct: 84 GGKVGYTSEMKFIPESSSRRIPCYRVLDEDGRIIPDSDFIPVSEKLAVRMYEQMATLQVM 143
Query: 435 DKILYESQRQGRISFYMTNYGEEGIT*AAPRHSHRKIWSSVQYREVGVFLYRGMTVTELV 614
D I YE+QRQGRISFY+T+ GEE I A+ QYRE GV L+RG T+ E
Sbjct: 144 DHIFYEAQRQGRISFYLTSVGEEAINIASAAALSPDDVVLPQYREPGVLLWRGFTLEEFA 203
Query: 615 NQCYGNCEDPGKGRQMPVHYGSKHHNMVTI 704
NQC+GN D GKGRQMP+HYGS N TI
Sbjct: 204 NQCFGNKADYGKGRQMPIHYGSNRLNYFTI 233
>At5g09300.2 68418.m01077 2-oxoisovalerate dehydrogenase, putative /
3-methyl-2-oxobutanoate dehydrogenase, putative /
branched-chain alpha-keto acid dehydrogenase E1 alpha
subunit, putative similar to branched-chain alpha-keto
acid dehydrogenase E1-alpha subunit [Gallus gallus]
GI:12964598; contains Pfam profile PF00676:
Dehydrogenase E1 component
Length = 401
Score = 142 bits (345), Expect = 2e-34
Identities = 68/150 (45%), Positives = 92/150 (61%)
Frame = +3
Query: 255 GARAPYVSEMKFFNETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKTMVQLSHM 434
G + + E++F +E+ E +P YRV+D+NGQ+I ++ + + + +Y MV L M
Sbjct: 13 GGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDMVTLQIM 72
Query: 435 DKILYESQRQGRISFYMTNYGEEGIT*AAPRHSHRKIWSSVQYREVGVFLYRGMTVTELV 614
D I YE+QRQGR+SFY T GEE I A+ + QYRE GV L+RG T+ E
Sbjct: 73 DNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGFTLQEFA 132
Query: 615 NQCYGNCEDPGKGRQMPVHYGSKHHNMVTI 704
NQC+GN D GKGRQMPVHYGS N T+
Sbjct: 133 NQCFGNKSDYGKGRQMPVHYGSNKLNYFTV 162
>At5g09300.1 68418.m01078 2-oxoisovalerate dehydrogenase, putative /
3-methyl-2-oxobutanoate dehydrogenase, putative /
branched-chain alpha-keto acid dehydrogenase E1 alpha
subunit, putative similar to branched-chain alpha-keto
acid dehydrogenase E1-alpha subunit [Gallus gallus]
GI:12964598; contains Pfam profile PF00676:
Dehydrogenase E1 component
Length = 472
Score = 142 bits (345), Expect = 2e-34
Identities = 68/150 (45%), Positives = 92/150 (61%)
Frame = +3
Query: 255 GARAPYVSEMKFFNETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKTMVQLSHM 434
G + + E++F +E+ E +P YRV+D+NGQ+I ++ + + + +Y MV L M
Sbjct: 84 GGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDMVTLQIM 143
Query: 435 DKILYESQRQGRISFYMTNYGEEGIT*AAPRHSHRKIWSSVQYREVGVFLYRGMTVTELV 614
D I YE+QRQGR+SFY T GEE I A+ + QYRE GV L+RG T+ E
Sbjct: 144 DNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGFTLQEFA 203
Query: 615 NQCYGNCEDPGKGRQMPVHYGSKHHNMVTI 704
NQC+GN D GKGRQMPVHYGS N T+
Sbjct: 204 NQCFGNKSDYGKGRQMPVHYGSNKLNYFTV 233
>At1g04140.2 68414.m00404 transducin family protein / WD-40 repeat
family protein contains 4 WD-40 repeats (PF00400);
similar to neural cell adhesion molecule 2, large
isoform precursor gb|M76710 from Xenopus laevis, and
beta transducin from S. cerevisiae gb|Q05946. ESTs
gb|N65081 gb|Z30910, gb|Z34190, gb|Z34611, gb|R30101,
gb|H36304, and gb|N65606 come from
Length = 793
Score = 32.7 bits (71), Expect = 0.26
Identities = 19/50 (38%), Positives = 24/50 (48%), Gaps = 3/50 (6%)
Frame = -2
Query: 725 CSLMELDNCD---HVVVLASVMDRHLSPFPRILAVSVTLVHKFRHSHPSV 585
CS+++ D C H VL S M H SP R LA V V + PS+
Sbjct: 570 CSILKSDKCRLTIHHAVLCSEMGAHFSPCGRYLAACVACVIPHAETDPSL 619
>At1g04140.1 68414.m00403 transducin family protein / WD-40 repeat
family protein contains 4 WD-40 repeats (PF00400);
similar to neural cell adhesion molecule 2, large
isoform precursor gb|M76710 from Xenopus laevis, and
beta transducin from S. cerevisiae gb|Q05946. ESTs
gb|N65081 gb|Z30910, gb|Z34190, gb|Z34611, gb|R30101,
gb|H36304, and gb|N65606 come from
Length = 790
Score = 32.7 bits (71), Expect = 0.26
Identities = 19/50 (38%), Positives = 24/50 (48%), Gaps = 3/50 (6%)
Frame = -2
Query: 725 CSLMELDNCD---HVVVLASVMDRHLSPFPRILAVSVTLVHKFRHSHPSV 585
CS+++ D C H VL S M H SP R LA V V + PS+
Sbjct: 570 CSILKSDKCRLTIHHAVLCSEMGAHFSPCGRYLAACVACVIPHAETDPSL 619
>At5g35200.1 68418.m04171 epsin N-terminal homology (ENTH)
domain-containing protein contains Pfam PF01417: ENTH
domain. ENTH (Epsin N-terminal homology) domain; similar
to clathrin assembly protein short form, Rattus
norvegicus, EMBL:AF041373;similar to Chain A, Calm-N
N-Terminal Domain Of Clathrin Assembly Lymphoid Myeloid
Leukaemia Protein, Pi(4,5)p2 Complex (GP:13399999) {Homo
sapiens}
Length = 544
Score = 28.3 bits (60), Expect = 5.7
Identities = 15/60 (25%), Positives = 24/60 (40%)
Frame = +3
Query: 294 NETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKTMVQLSHMDKILYESQRQGRI 473
N T +E + N G D LDK TL ++Y+ +++S Y Q +
Sbjct: 401 NSTGWELALVTAPSSNEGAAADSKLAGGLDKLTLDSLYEDAIRVSQQQNRSYNPWEQNPV 460
>At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alpha
subunit, mitochondrial (PDHE1-A) identical to SP|P52901
Pyruvate dehydrogenase E1 component alpha subunit,
mitochondrial precursor (EC 1.2.4.1) (PDHE1-A)
{Arabidopsis thaliana}
Length = 389
Score = 28.3 bits (60), Expect = 5.7
Identities = 30/150 (20%), Positives = 59/150 (39%), Gaps = 3/150 (2%)
Frame = +3
Query: 243 SRISGARAPYVSEMKFFNETSYEPIPIYRVMDNNGQIIDK-NEEPNLDKATLINMYKTMV 419
SR + P+ + T PI I + + D + L++ ++TM
Sbjct: 8 SRSNIITRPFSAAFSRLISTDTTPITIETSLPFTAHLCDPPSRSVESSSQELLDFFRTMA 67
Query: 420 QLSHMDKILYESQRQGRI--SFYMTNYGEEGIT*AAPRHSHRKIWSSVQYREVGVFLYRG 593
+ M+ I +S + ++ F G+E + +K YR+ +FL RG
Sbjct: 68 LMRRME-IAADSLYKAKLIRGFCHLYDGQEAVAIGMEAAITKKDAIITAYRDHCIFLGRG 126
Query: 594 MTVTELVNQCYGNCEDPGKGRQMPVHYGSK 683
++ E+ ++ G KG+ +H+ K
Sbjct: 127 GSLHEVFSELMGRQAGCSKGKGGSMHFYKK 156
>At5g49660.1 68418.m06147 leucine-rich repeat transmembrane protein
kinase, putative contains leucine rich repeat (LRR)
domains, Pfam:PF00560; contains protein kinase domain,
Pfam:PF00069
Length = 966
Score = 27.9 bits (59), Expect = 7.5
Identities = 16/54 (29%), Positives = 29/54 (53%)
Frame = +3
Query: 273 VSEMKFFNETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKTMVQLSHM 434
V +M + + T P+ I+ + D + ++ NE P LD TL + + +L+HM
Sbjct: 149 VIDMSWNHFTGSFPLSIFNLTDL--EYLNFNENPELDLWTLPDSVSKLTKLTHM 200
>At5g24470.1 68418.m02884 pseudo-response regulator 5 (APRR5)
identical to pseudo-response regulator 5 GI:10281006
from [Arabidopsis thaliana]
Length = 667
Score = 27.9 bits (59), Expect = 7.5
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = -3
Query: 226 SVPFRASYASTKAISRYLLSIFFSHHYF 143
SV S + K +R+ L IFFS HY+
Sbjct: 29 SVRHPLSIITVKTFARFFLDIFFSPHYY 56
>At4g02590.1 68417.m00353 basic helix-loop-helix (bHLH) family
protein similar to A. thaliana putative protein
F6I18.110, GenBank accession number 2980768
Length = 310
Score = 27.9 bits (59), Expect = 7.5
Identities = 15/39 (38%), Positives = 20/39 (51%)
Frame = +2
Query: 605 GTCEPVLRKLRGSGERETDAGPLRKQAPQHGHNYLVPLG 721
G P++ +L GSGE + G L P HN + PLG
Sbjct: 46 GGAPPMMLQL-GSGEEGSHMGGLGGSGPTGFHNQMFPLG 83
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,424,173
Number of Sequences: 28952
Number of extensions: 386883
Number of successful extensions: 942
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 914
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 938
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1633819784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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