BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesV0336.Seq (741 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g21400.1 68414.m02678 2-oxoisovalerate dehydrogenase, putativ... 145 2e-35 At5g09300.2 68418.m01077 2-oxoisovalerate dehydrogenase, putativ... 142 2e-34 At5g09300.1 68418.m01078 2-oxoisovalerate dehydrogenase, putativ... 142 2e-34 At1g04140.2 68414.m00404 transducin family protein / WD-40 repea... 33 0.26 At1g04140.1 68414.m00403 transducin family protein / WD-40 repea... 33 0.26 At5g35200.1 68418.m04171 epsin N-terminal homology (ENTH) domain... 28 5.7 At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alp... 28 5.7 At5g49660.1 68418.m06147 leucine-rich repeat transmembrane prote... 28 7.5 At5g24470.1 68418.m02884 pseudo-response regulator 5 (APRR5) ide... 28 7.5 At4g02590.1 68417.m00353 basic helix-loop-helix (bHLH) family pr... 28 7.5 >At1g21400.1 68414.m02678 2-oxoisovalerate dehydrogenase, putative / 3-methyl-2-oxobutanoate dehydrogenase, putative / branched-chain alpha-keto acid dehydrogenase E1 alpha subunit, putative similar to branched-chain alpha-keto acid dehydrogenase E1-alpha subunit [Gallus gallus] GI:12964598; contains Pfam profile PF00676: Dehydrogenase E1 component Length = 472 Score = 145 bits (352), Expect = 2e-35 Identities = 73/150 (48%), Positives = 92/150 (61%) Frame = +3 Query: 255 GARAPYVSEMKFFNETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKTMVQLSHM 434 G + Y SEMKF E+S IP YRV+D +G+II ++ + + + MY+ M L M Sbjct: 84 GGKVGYTSEMKFIPESSSRRIPCYRVLDEDGRIIPDSDFIPVSEKLAVRMYEQMATLQVM 143 Query: 435 DKILYESQRQGRISFYMTNYGEEGIT*AAPRHSHRKIWSSVQYREVGVFLYRGMTVTELV 614 D I YE+QRQGRISFY+T+ GEE I A+ QYRE GV L+RG T+ E Sbjct: 144 DHIFYEAQRQGRISFYLTSVGEEAINIASAAALSPDDVVLPQYREPGVLLWRGFTLEEFA 203 Query: 615 NQCYGNCEDPGKGRQMPVHYGSKHHNMVTI 704 NQC+GN D GKGRQMP+HYGS N TI Sbjct: 204 NQCFGNKADYGKGRQMPIHYGSNRLNYFTI 233 >At5g09300.2 68418.m01077 2-oxoisovalerate dehydrogenase, putative / 3-methyl-2-oxobutanoate dehydrogenase, putative / branched-chain alpha-keto acid dehydrogenase E1 alpha subunit, putative similar to branched-chain alpha-keto acid dehydrogenase E1-alpha subunit [Gallus gallus] GI:12964598; contains Pfam profile PF00676: Dehydrogenase E1 component Length = 401 Score = 142 bits (345), Expect = 2e-34 Identities = 68/150 (45%), Positives = 92/150 (61%) Frame = +3 Query: 255 GARAPYVSEMKFFNETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKTMVQLSHM 434 G + + E++F +E+ E +P YRV+D+NGQ+I ++ + + + +Y MV L M Sbjct: 13 GGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDMVTLQIM 72 Query: 435 DKILYESQRQGRISFYMTNYGEEGIT*AAPRHSHRKIWSSVQYREVGVFLYRGMTVTELV 614 D I YE+QRQGR+SFY T GEE I A+ + QYRE GV L+RG T+ E Sbjct: 73 DNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGFTLQEFA 132 Query: 615 NQCYGNCEDPGKGRQMPVHYGSKHHNMVTI 704 NQC+GN D GKGRQMPVHYGS N T+ Sbjct: 133 NQCFGNKSDYGKGRQMPVHYGSNKLNYFTV 162 >At5g09300.1 68418.m01078 2-oxoisovalerate dehydrogenase, putative / 3-methyl-2-oxobutanoate dehydrogenase, putative / branched-chain alpha-keto acid dehydrogenase E1 alpha subunit, putative similar to branched-chain alpha-keto acid dehydrogenase E1-alpha subunit [Gallus gallus] GI:12964598; contains Pfam profile PF00676: Dehydrogenase E1 component Length = 472 Score = 142 bits (345), Expect = 2e-34 Identities = 68/150 (45%), Positives = 92/150 (61%) Frame = +3 Query: 255 GARAPYVSEMKFFNETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKTMVQLSHM 434 G + + E++F +E+ E +P YRV+D+NGQ+I ++ + + + +Y MV L M Sbjct: 84 GGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDMVTLQIM 143 Query: 435 DKILYESQRQGRISFYMTNYGEEGIT*AAPRHSHRKIWSSVQYREVGVFLYRGMTVTELV 614 D I YE+QRQGR+SFY T GEE I A+ + QYRE GV L+RG T+ E Sbjct: 144 DNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGFTLQEFA 203 Query: 615 NQCYGNCEDPGKGRQMPVHYGSKHHNMVTI 704 NQC+GN D GKGRQMPVHYGS N T+ Sbjct: 204 NQCFGNKSDYGKGRQMPVHYGSNKLNYFTV 233 >At1g04140.2 68414.m00404 transducin family protein / WD-40 repeat family protein contains 4 WD-40 repeats (PF00400); similar to neural cell adhesion molecule 2, large isoform precursor gb|M76710 from Xenopus laevis, and beta transducin from S. cerevisiae gb|Q05946. ESTs gb|N65081 gb|Z30910, gb|Z34190, gb|Z34611, gb|R30101, gb|H36304, and gb|N65606 come from Length = 793 Score = 32.7 bits (71), Expect = 0.26 Identities = 19/50 (38%), Positives = 24/50 (48%), Gaps = 3/50 (6%) Frame = -2 Query: 725 CSLMELDNCD---HVVVLASVMDRHLSPFPRILAVSVTLVHKFRHSHPSV 585 CS+++ D C H VL S M H SP R LA V V + PS+ Sbjct: 570 CSILKSDKCRLTIHHAVLCSEMGAHFSPCGRYLAACVACVIPHAETDPSL 619 >At1g04140.1 68414.m00403 transducin family protein / WD-40 repeat family protein contains 4 WD-40 repeats (PF00400); similar to neural cell adhesion molecule 2, large isoform precursor gb|M76710 from Xenopus laevis, and beta transducin from S. cerevisiae gb|Q05946. ESTs gb|N65081 gb|Z30910, gb|Z34190, gb|Z34611, gb|R30101, gb|H36304, and gb|N65606 come from Length = 790 Score = 32.7 bits (71), Expect = 0.26 Identities = 19/50 (38%), Positives = 24/50 (48%), Gaps = 3/50 (6%) Frame = -2 Query: 725 CSLMELDNCD---HVVVLASVMDRHLSPFPRILAVSVTLVHKFRHSHPSV 585 CS+++ D C H VL S M H SP R LA V V + PS+ Sbjct: 570 CSILKSDKCRLTIHHAVLCSEMGAHFSPCGRYLAACVACVIPHAETDPSL 619 >At5g35200.1 68418.m04171 epsin N-terminal homology (ENTH) domain-containing protein contains Pfam PF01417: ENTH domain. ENTH (Epsin N-terminal homology) domain; similar to clathrin assembly protein short form, Rattus norvegicus, EMBL:AF041373;similar to Chain A, Calm-N N-Terminal Domain Of Clathrin Assembly Lymphoid Myeloid Leukaemia Protein, Pi(4,5)p2 Complex (GP:13399999) {Homo sapiens} Length = 544 Score = 28.3 bits (60), Expect = 5.7 Identities = 15/60 (25%), Positives = 24/60 (40%) Frame = +3 Query: 294 NETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKTMVQLSHMDKILYESQRQGRI 473 N T +E + N G D LDK TL ++Y+ +++S Y Q + Sbjct: 401 NSTGWELALVTAPSSNEGAAADSKLAGGLDKLTLDSLYEDAIRVSQQQNRSYNPWEQNPV 460 >At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alpha subunit, mitochondrial (PDHE1-A) identical to SP|P52901 Pyruvate dehydrogenase E1 component alpha subunit, mitochondrial precursor (EC 1.2.4.1) (PDHE1-A) {Arabidopsis thaliana} Length = 389 Score = 28.3 bits (60), Expect = 5.7 Identities = 30/150 (20%), Positives = 59/150 (39%), Gaps = 3/150 (2%) Frame = +3 Query: 243 SRISGARAPYVSEMKFFNETSYEPIPIYRVMDNNGQIIDK-NEEPNLDKATLINMYKTMV 419 SR + P+ + T PI I + + D + L++ ++TM Sbjct: 8 SRSNIITRPFSAAFSRLISTDTTPITIETSLPFTAHLCDPPSRSVESSSQELLDFFRTMA 67 Query: 420 QLSHMDKILYESQRQGRI--SFYMTNYGEEGIT*AAPRHSHRKIWSSVQYREVGVFLYRG 593 + M+ I +S + ++ F G+E + +K YR+ +FL RG Sbjct: 68 LMRRME-IAADSLYKAKLIRGFCHLYDGQEAVAIGMEAAITKKDAIITAYRDHCIFLGRG 126 Query: 594 MTVTELVNQCYGNCEDPGKGRQMPVHYGSK 683 ++ E+ ++ G KG+ +H+ K Sbjct: 127 GSLHEVFSELMGRQAGCSKGKGGSMHFYKK 156 >At5g49660.1 68418.m06147 leucine-rich repeat transmembrane protein kinase, putative contains leucine rich repeat (LRR) domains, Pfam:PF00560; contains protein kinase domain, Pfam:PF00069 Length = 966 Score = 27.9 bits (59), Expect = 7.5 Identities = 16/54 (29%), Positives = 29/54 (53%) Frame = +3 Query: 273 VSEMKFFNETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKTMVQLSHM 434 V +M + + T P+ I+ + D + ++ NE P LD TL + + +L+HM Sbjct: 149 VIDMSWNHFTGSFPLSIFNLTDL--EYLNFNENPELDLWTLPDSVSKLTKLTHM 200 >At5g24470.1 68418.m02884 pseudo-response regulator 5 (APRR5) identical to pseudo-response regulator 5 GI:10281006 from [Arabidopsis thaliana] Length = 667 Score = 27.9 bits (59), Expect = 7.5 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = -3 Query: 226 SVPFRASYASTKAISRYLLSIFFSHHYF 143 SV S + K +R+ L IFFS HY+ Sbjct: 29 SVRHPLSIITVKTFARFFLDIFFSPHYY 56 >At4g02590.1 68417.m00353 basic helix-loop-helix (bHLH) family protein similar to A. thaliana putative protein F6I18.110, GenBank accession number 2980768 Length = 310 Score = 27.9 bits (59), Expect = 7.5 Identities = 15/39 (38%), Positives = 20/39 (51%) Frame = +2 Query: 605 GTCEPVLRKLRGSGERETDAGPLRKQAPQHGHNYLVPLG 721 G P++ +L GSGE + G L P HN + PLG Sbjct: 46 GGAPPMMLQL-GSGEEGSHMGGLGGSGPTGFHNQMFPLG 83 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,424,173 Number of Sequences: 28952 Number of extensions: 386883 Number of successful extensions: 942 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 914 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 938 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1633819784 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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