BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesV0327.Seq (738 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g03660.1 68418.m00325 expressed protein low similarity to out... 31 1.1 At3g22790.1 68416.m02873 kinase interacting family protein simil... 29 3.2 At1g23030.1 68414.m02877 armadillo/beta-catenin repeat family pr... 29 4.3 At1g61410.1 68414.m06920 tolA protein-related contains weak simi... 28 5.6 At1g06490.1 68414.m00688 glycosyl transferase family 48 protein ... 28 7.4 At5g39500.1 68418.m04783 pattern formation protein, putative sim... 27 9.8 At1g58330.1 68414.m06635 transcription factor-related low simila... 27 9.8 At1g14690.1 68414.m01756 microtubule associated protein (MAP65/A... 27 9.8 >At5g03660.1 68418.m00325 expressed protein low similarity to outer surface protein F [Borrelia burgdorferi] GI:466482; contains Pfam profile PF04949: Family of unknown function (DUF662) Length = 173 Score = 30.7 bits (66), Expect = 1.1 Identities = 17/68 (25%), Positives = 35/68 (51%) Frame = +1 Query: 268 KRENSVVEKKLSTKPIDKYCKDIIQDIEKSSKVIDSHVKQFNNSRFESDKLVEQLQAVDK 447 ++E ++V KK+ + +DK K + ++K ++ FN E +L+ +LQ ++ Sbjct: 94 RKEVTMVRKKIDS--LDKELKPLGNTVQKKETEYKDALEAFNEKNKEKVELITKLQELEG 151 Query: 448 LNELVNFK 471 +E FK Sbjct: 152 ESEKFRFK 159 >At3g22790.1 68416.m02873 kinase interacting family protein similar to kinase interacting protein 1 (GI:13936326) [Petunia integrifolia] Length = 1694 Score = 29.1 bits (62), Expect = 3.2 Identities = 22/69 (31%), Positives = 39/69 (56%), Gaps = 2/69 (2%) Frame = +1 Query: 301 STKPIDKYCKDIIQDIEK--SSKVIDSHVKQFNNSRFESDKLVEQLQAVDKLNELVNFKG 474 S + I+ K ++ +E+ K +DS Q NS+ E KL E L+ ++LN+L+ + Sbjct: 1101 SLQNINSGLKQKVETLEEILKGKEVDS---QELNSKLE--KLQESLEEANELNDLLEHQI 1155 Query: 475 EIPEETLKE 501 + EETL++ Sbjct: 1156 LVKEETLRQ 1164 >At1g23030.1 68414.m02877 armadillo/beta-catenin repeat family protein / U-box domain-containing protein contains Pfam domain, PF00514: Armadillo/beta-catenin-like repeats and Pfam, PF04564: U-box domain Length = 612 Score = 28.7 bits (61), Expect = 4.3 Identities = 26/118 (22%), Positives = 49/118 (41%), Gaps = 4/118 (3%) Frame = +1 Query: 226 DNHSGGGK*CDFKFKRENSVVEKKLSTKPIDKY--CKDIIQDIEKSSKVIDSHVKQFN-- 393 D+ G K F+F+ +EK LS P D Y ++ + +E + + ++++ Sbjct: 91 DSSDGAAKRISFQFQCVTWKLEKALSNLPYDLYDISDEVGEQVELARSQLRRAMQRYGSL 150 Query: 394 NSRFESDKLVEQLQAVDKLNELVNFKGEIPEETLKELNXXXXXXXXXXXXPPRRENDL 567 NS S L E ++ D + ++ K E E++ E P RR + + Sbjct: 151 NSNKFSSALSEPMER-DGFSNVIKIKAEEKLESVSETLHFGEEEEKQSSPPLRRSSSI 207 >At1g61410.1 68414.m06920 tolA protein-related contains weak similarity to Swiss-Prot:P19934 TolA protein [Escherichia coli] Length = 118 Score = 28.3 bits (60), Expect = 5.6 Identities = 17/59 (28%), Positives = 26/59 (44%) Frame = +1 Query: 241 GGK*CDFKFKRENSVVEKKLSTKPIDKYCKDIIQDIEKSSKVIDSHVKQFNNSRFESDK 417 G K CD K + EN+ K LS K ++D E S + ++ + FES + Sbjct: 32 GEKLCDEKTEFENATYAKFLSVLNAKKAKLRAVRDKEDSVRAVEEEESTYKAESFESGR 90 >At1g06490.1 68414.m00688 glycosyl transferase family 48 protein contains Pfam profile: PF02364 1,3-beta-glucan synthase Length = 1933 Score = 27.9 bits (59), Expect = 7.4 Identities = 25/84 (29%), Positives = 41/84 (48%), Gaps = 2/84 (2%) Frame = -1 Query: 564 IVFSSGRYLELDQSKL*NYI-QFL*SFFWYFTLEID*FVQFIDGLQLLHKLIAFKSRIIK 388 +V S G + L S++ YI +FL + W L I + + + L K F S + Sbjct: 555 LVLSFGAWKSLKFSQIMRYITKFLMAAMWAIMLPIT-YSKSVQNPTGLIKF--FSSWVGS 611 Query: 387 LFYVTIYDFATLFYIL-NYIFAVF 319 + ++YD+A Y+L N + AVF Sbjct: 612 WLHRSLYDYAIALYVLPNILAAVF 635 >At5g39500.1 68418.m04783 pattern formation protein, putative similar to SP|Q42510 Pattern formation protein EMB30 {Arabidopsis thaliana}; contains Pfam profile PF01369: Sec7 domain Length = 1443 Score = 27.5 bits (58), Expect = 9.8 Identities = 12/30 (40%), Positives = 17/30 (56%) Frame = +1 Query: 295 KLSTKPIDKYCKDIIQDIEKSSKVIDSHVK 384 KL K D YC+ I Q++ + K SHV+ Sbjct: 1101 KLKAKVADAYCERIAQEVVRLVKANASHVR 1130 >At1g58330.1 68414.m06635 transcription factor-related low similarity to tumor-related protein [Nicotiana glauca x Nicotiana langsdorffii] GI:688423, ocs-element binding factor 4 [Arabidopsis thaliana] GI:414613, mas-binding factor MBF3 [Solanum tuberosum] GI:13195751; supporting cDNA gi|6520153|dbj|AB028196.1| Length = 225 Score = 27.5 bits (58), Expect = 9.8 Identities = 10/22 (45%), Positives = 16/22 (72%) Frame = +2 Query: 8 EMQKRMEAMKIELINKIKNNDE 73 EM+ ME +K+E+I +KN D+ Sbjct: 167 EMEDAMEVLKVEMIKAMKNADQ 188 >At1g14690.1 68414.m01756 microtubule associated protein (MAP65/ASE1) family protein low similarity to SP|P32380 NUF1 protein (Spindle poly body spacer protein SPC110) {Saccharomyces cerevisiae}, smooth muscle myosin heavy chain [Homo sapiens] GI:4417214; contains Pfam profile PF03999: Microtubule associated protein (MAP65/ASE1 family) Length = 707 Score = 27.5 bits (58), Expect = 9.8 Identities = 20/59 (33%), Positives = 25/59 (42%) Frame = +1 Query: 331 DIIQDIEKSSKVIDSHVKQFNNSRFESDKLVEQLQAVDKLNELVNFKGEIPEETLKELN 507 DI IEK S I + Q N + S L EQ + KLNE + +E LN Sbjct: 114 DIKAQIEKMSGEISGYSDQLNKTMVGSLALDEQDLTLRKLNEYQTHLRSLQKEKSDRLN 172 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,675,822 Number of Sequences: 28952 Number of extensions: 221141 Number of successful extensions: 586 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 574 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 586 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1624036432 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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