Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesV0326.Seq
(735 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 118 4e-27
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 118 4e-27
At5g17650.1 68418.m02069 glycine/proline-rich protein glycine/pr... 31 0.79
At1g26400.1 68414.m03220 FAD-binding domain-containing protein s... 30 1.8
At1g57750.1 68414.m06552 cytochrome P450, putative similar to cy... 29 2.4
At3g50110.1 68416.m05478 phosphatase-related similar to PTEN1 GI... 29 3.2
At2g43680.2 68415.m05430 calmodulin-binding family protein simil... 29 3.2
At2g43680.1 68415.m05429 calmodulin-binding family protein simil... 29 3.2
At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin; g... 29 4.2
At4g39480.1 68417.m05585 cytochrome P450 family protein contains... 28 5.6
At4g25170.1 68417.m03624 expressed protein 28 7.4
At4g08960.1 68417.m01476 phosphotyrosyl phosphatase activator (P... 28 7.4
At3g28720.1 68416.m03586 expressed protein 27 9.8
At3g23670.1 68416.m02976 phragmoplast-associated kinesin-related... 27 9.8
At1g76010.1 68414.m08825 expressed protein 27 9.8
At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to S... 27 9.8
At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to S... 27 9.8
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 118 bits (284), Expect = 4e-27
Identities = 55/91 (60%), Positives = 57/91 (62%)
Frame = +2
Query: 254 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 433
QTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 67 QTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMK 126
Query: 434 XXXXXXXXXXXXXXXXXQARGHIIERFPSFP 526
ARGH IE P P
Sbjct: 127 RHAIVSAIAATAVPALVMARGHKIENVPEMP 157
Score = 66.1 bits (154), Expect = 2e-11
Identities = 33/63 (52%), Positives = 41/63 (65%), Gaps = 2/63 (3%)
Frame = +3
Query: 75 SVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEA 248
+ ARPLV+V + T Q LP V AP+RPD+VN VH +S NSRQPY VSK+A
Sbjct: 5 AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKA 64
Query: 249 GHK 257
GH+
Sbjct: 65 GHQ 67
Score = 53.6 bits (123), Expect = 1e-07
Identities = 27/63 (42%), Positives = 40/63 (63%)
Frame = +1
Query: 511 IPELPLVVADKVQEINKTKQAVIFLRRLQGMV*ISLRCTSXQRLRAGKGKMRNRRRIQRK 690
+PE+PLVV+D + + KT A+ L+++ G + + + +R GKGKMRNRR I RK
Sbjct: 153 VPEMPLVVSDSAEAVEKTSAAIKVLKQI-GAYDDAEKAKNSIGIRPGKGKMRNRRYISRK 211
Query: 691 GXL 699
G L
Sbjct: 212 GPL 214
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 118 bits (284), Expect = 4e-27
Identities = 55/91 (60%), Positives = 57/91 (62%)
Frame = +2
Query: 254 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 433
QTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 66 QTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMK 125
Query: 434 XXXXXXXXXXXXXXXXXQARGHIIERFPSFP 526
ARGH IE P P
Sbjct: 126 RHAIVSAIAATAVPALVMARGHKIENVPEMP 156
Score = 68.1 bits (159), Expect = 6e-12
Identities = 33/66 (50%), Positives = 45/66 (68%), Gaps = 2/66 (3%)
Frame = +3
Query: 66 MSLSVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVS 239
M+ + ARPLV++ + + T Q + LP V AP+RPD+VN VH +S NSRQPY VS
Sbjct: 1 MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60
Query: 240 KEAGHK 257
K+AGH+
Sbjct: 61 KKAGHQ 66
Score = 53.6 bits (123), Expect = 1e-07
Identities = 27/63 (42%), Positives = 40/63 (63%)
Frame = +1
Query: 511 IPELPLVVADKVQEINKTKQAVIFLRRLQGMV*ISLRCTSXQRLRAGKGKMRNRRRIQRK 690
+PE+PLVV+D + + KT A+ L+++ G + + + +R GKGKMRNRR I RK
Sbjct: 152 VPEMPLVVSDSAEAVEKTSAAIKVLKQI-GAYDDAEKAKNSIGIRPGKGKMRNRRYISRK 210
Query: 691 GXL 699
G L
Sbjct: 211 GPL 213
>At5g17650.1 68418.m02069 glycine/proline-rich protein
glycine/proline-rich protein GPRP - Arabidopsis
thaliana, EMBL:X84315
Length = 173
Score = 31.1 bits (67), Expect = 0.79
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = -2
Query: 368 HHDTCYRRHPDRTYGYHHHGHAEF 297
HH Y H YGY +HGH +F
Sbjct: 122 HHHGHYGHHHGHGYGYGYHGHGKF 145
>At1g26400.1 68414.m03220 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 527
Score = 29.9 bits (64), Expect = 1.8
Identities = 14/25 (56%), Positives = 17/25 (68%)
Frame = -2
Query: 569 CLVLLISWTLSATTKGSSGIFQ*CV 495
CLVLL+S +A TK SGIF C+
Sbjct: 9 CLVLLVSILRAAVTKPDSGIFTGCL 33
>At1g57750.1 68414.m06552 cytochrome P450, putative similar to
cytochrome P450 GI:4688670 from [Catharanthus roseus]
Length = 497
Score = 29.5 bits (63), Expect = 2.4
Identities = 16/49 (32%), Positives = 29/49 (59%)
Frame = +3
Query: 90 LVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 236
LV +++ SE+++ PLPF K+P +PD++ H + NS+ C+
Sbjct: 351 LVYLHAALSESMR-LYPPLPFNHKSPAKPDVLPSGH-KVDANSKIVICI 397
>At3g50110.1 68416.m05478 phosphatase-related similar to PTEN1
GI:5566292 from [Drosophila melanogaster]; contains
prosite evidence: PS00383: Tyrosine specific protein
phosphatases active site
Length = 632
Score = 29.1 bits (62), Expect = 3.2
Identities = 16/45 (35%), Positives = 22/45 (48%)
Frame = -3
Query: 526 REARESFNNVSSSLNERWDASSSNGCCQGRSPLSEVDATVPAPPG 392
REA E + +S+ + D + G PLS+ D VP PPG
Sbjct: 42 REAHEDSISTEASV-AKVDDTQMPASSTGSEPLSKTDDIVPCPPG 85
>At2g43680.2 68415.m05430 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 669
Score = 29.1 bits (62), Expect = 3.2
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +1
Query: 364 WWTYVRPHEALAALAPSRQPPTAESGLGSSRCC 462
WW +V LA+ APS P + L SR C
Sbjct: 465 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC 497
>At2g43680.1 68415.m05429 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 668
Score = 29.1 bits (62), Expect = 3.2
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +1
Query: 364 WWTYVRPHEALAALAPSRQPPTAESGLGSSRCC 462
WW +V LA+ APS P + L SR C
Sbjct: 464 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC 496
>At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin;
glycine-rich protein 18 (GRP18) PMID:11431566;
Length = 228
Score = 28.7 bits (61), Expect = 4.2
Identities = 18/64 (28%), Positives = 30/64 (46%), Gaps = 3/64 (4%)
Frame = +2
Query: 215 LEAALLREQGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQ---GAFGNMCRGGRMFAP 385
+ AL+R + G ++ +AE + + + ++P G G G+ G FGN GG F
Sbjct: 76 MAVALIRRRMG-AKPTAEGTSSAQPLLKLPVYGGYGGFWGGKKFSGTFGNKPGGGNPFGD 134
Query: 386 TKPW 397
W
Sbjct: 135 ISKW 138
>At4g39480.1 68417.m05585 cytochrome P450 family protein contains
Pfam profile: PF00067 cytochrome P450
Length = 989
Score = 28.3 bits (60), Expect = 5.6
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +3
Query: 141 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 236
P+PF K+P +PD++ H + NSR +C+
Sbjct: 384 PVPFNHKSPAKPDVLPSGH-KVKANSRILFCL 414
Score = 27.5 bits (58), Expect = 9.8
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +3
Query: 141 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 236
P+PF K+P +PD++ H + NS+ +C+
Sbjct: 857 PVPFQHKSPTKPDVLPSGH-KVDANSKILFCL 887
>At4g25170.1 68417.m03624 expressed protein
Length = 344
Score = 27.9 bits (59), Expect = 7.4
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = -1
Query: 615 GYSDHALKAPQEDDSLFGLVDLLDFV 538
GY D + +EDDSLFG V +D V
Sbjct: 103 GYDDFEWVSEEEDDSLFGSVPSVDEV 128
>At4g08960.1 68417.m01476 phosphotyrosyl phosphatase activator
(PTPA) family protein similar to Protein phosphatase 2A,
regulatory subunit B' (PP2A, subunit B', PR53 isoform)
(Phosphotyrosyl phosphatase activator) (PTPA)
(Swiss-Prot:Q28717) [Oryctolagus cuniculus]
Length = 392
Score = 27.9 bits (59), Expect = 7.4
Identities = 15/36 (41%), Positives = 20/36 (55%)
Frame = -1
Query: 351 PKAP*PDLWVPPPRTRGIRATARPVPHDSALVCDQP 244
P A P++ PPP R IRA A +PH+S + P
Sbjct: 46 PLASFPEM-SPPPNYRPIRAPAINLPHNSQAIILSP 80
>At3g28720.1 68416.m03586 expressed protein
Length = 687
Score = 27.5 bits (58), Expect = 9.8
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = -3
Query: 538 RLQPREARESFNNVSSSLNERWDASSSNGCCQGRSPLS 425
RL P+ + +SF ++SSSL +SS + +S LS
Sbjct: 46 RLDPKSSNDSFTSLSSSLKRSLSSSSIHFSSLSKSLLS 83
>At3g23670.1 68416.m02976 phragmoplast-associated kinesin-related
protein, putative similar to kinesin like protein
GB:CAB10194 from [Arabidopsis thaliana]
Length = 1313
Score = 27.5 bits (58), Expect = 9.8
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -3
Query: 514 ESFNNVSSSLNERWDASSSNGCCQGRSPLSEVD 416
ES+NN +E D + + CCQ + SE D
Sbjct: 557 ESYNNSHLKSSEATDVNMEDACCQTENNGSETD 589
>At1g76010.1 68414.m08825 expressed protein
Length = 350
Score = 27.5 bits (58), Expect = 9.8
Identities = 17/43 (39%), Positives = 18/43 (41%)
Frame = +2
Query: 239 QGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRG 367
QG + G GR R R RGGG R G G F N G
Sbjct: 303 QGRGGYDGPQGRGRGRGRGRGGRGRGGG--RGGDGGFNNRSDG 343
>At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1050
Score = 27.5 bits (58), Expect = 9.8
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +2
Query: 239 QGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGR 373
+G S+ + G GR R +GGG H+ G+G +GGR
Sbjct: 15 EGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGR 59
>At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1048
Score = 27.5 bits (58), Expect = 9.8
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +2
Query: 239 QGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGR 373
+G S+ + G GR R +GGG H+ G+G +GGR
Sbjct: 15 EGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGR 59
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,018,172
Number of Sequences: 28952
Number of extensions: 321459
Number of successful extensions: 1112
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1035
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1100
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1614253080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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