Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= tesV0316.Seq
         (732 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   149   4e-37
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...    58   1e-09
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...    54   2e-08
SPBP4H10.04 |ppb1||calcineurin catalytic subunit Ppb1|Schizosacc...    30   0.39 
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    29   0.68 
SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyce...    26   6.4  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  149 bits (361), Expect = 4e-37
 Identities = 70/130 (53%), Positives = 87/130 (66%)
 Frame = -3

Query: 646 VNMVPVPAIALFHTGFRTSDVTRESAVPCXXXXXXXXXXX*REEHDAACDPRHGRYLTVA 467
           VNMVP P +  F  GF        S+                     A DPRHGRYLTVA
Sbjct: 255 VNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPRHGRYLTVA 314

Query: 466 AVFRGRMSMKEVDEQMMNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAI 287
           A+FRG++SMKEVDEQ+ ++Q KNS+YFVEWIP+NV  AVC +PP+ LKMSATFIGNST+I
Sbjct: 315 ALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPKDLKMSATFIGNSTSI 374

Query: 286 QELFKRISEQ 257
           QE+F+R+ +Q
Sbjct: 375 QEIFRRLGDQ 384



 Score = 91.5 bits (217), Expect = 1e-19
 Identities = 40/41 (97%), Positives = 41/41 (100%)
 Frame = -2

Query: 254 TAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 132
           +AMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ
Sbjct: 386 SAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 426



 Score = 25.4 bits (53), Expect = 8.4
 Identities = 10/14 (71%), Positives = 11/14 (78%)
 Frame = -2

Query: 722 ATMSGVTTCLRFPG 681
           A M+GVTT  RFPG
Sbjct: 231 AVMAGVTTSFRFPG 244


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score = 58.4 bits (135), Expect = 1e-09
 Identities = 27/90 (30%), Positives = 47/90 (52%), Gaps = 8/90 (8%)
 Frame = -3

Query: 502 CDPRHGRYLTVAAVFRGRMSMKEVDEQMMNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLK 323
           CDPR GRY+    ++RG +  ++V   +  I+ K +  FV+W P   K  +CD PP+ ++
Sbjct: 305 CDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIE 364

Query: 322 MS--------ATFIGNSTAIQELFKRISEQ 257
            S           + N+T+I E + R+  +
Sbjct: 365 GSEIAKVDRAVCMLSNTTSIAEAWSRLDHK 394



 Score = 47.6 bits (108), Expect = 2e-06
 Identities = 17/37 (45%), Positives = 28/37 (75%)
 Frame = -2

Query: 248 MFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 138
           M+ ++AF+HWY GEGM+E EF+EA  ++  L  +Y++
Sbjct: 398 MYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEE 434



 Score = 37.5 bits (83), Expect = 0.002
 Identities = 15/42 (35%), Positives = 25/42 (59%)
 Frame = -1

Query: 633 PFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMM 508
           P+PR+HF +  +AP+ S     + + +V E+T Q F+  N M
Sbjct: 261 PYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQM 302


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score = 54.0 bits (124), Expect = 2e-08
 Identities = 24/90 (26%), Positives = 47/90 (52%), Gaps = 8/90 (8%)
 Frame = -3

Query: 502 CDPRHGRYLTVAAVFRGRMSMKEVDEQMMNIQNKNSSYFVEWIPNNVKTAVCDIPPR--- 332
           CDPR GRY+    ++RG +  ++V   + +I+++ +  FV+W P   K  +C  PP+   
Sbjct: 309 CDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVP 368

Query: 331 -----GLKMSATFIGNSTAIQELFKRISEQ 257
                 +  +   + N+T+I E + R+  +
Sbjct: 369 GSGIAKVNRAVCMLSNTTSIAEAWSRLDHK 398



 Score = 47.6 bits (108), Expect = 2e-06
 Identities = 17/37 (45%), Positives = 28/37 (75%)
 Frame = -2

Query: 248 MFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 138
           M+ ++AF+HWY GEGM+E EF+EA  ++  L  +Y++
Sbjct: 402 MYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEE 438



 Score = 36.3 bits (80), Expect = 0.005
 Identities = 14/42 (33%), Positives = 25/42 (59%)
 Frame = -1

Query: 633 PFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMM 508
           P+PR+HF +  ++P+ S     + + +V E+T Q F+  N M
Sbjct: 265 PYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQM 306


>SPBP4H10.04 |ppb1||calcineurin catalytic subunit
           Ppb1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 554

 Score = 29.9 bits (64), Expect = 0.39
 Identities = 17/66 (25%), Positives = 33/66 (50%), Gaps = 2/66 (3%)
 Frame = -3

Query: 463 VFRGRMSMKEVDEQMMNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIG--NSTA 290
           V+  + ++ + +  +MNI+  N S    W+PN +      +P  G K+S   I   N  +
Sbjct: 343 VYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVSEMLISMLNICS 402

Query: 289 IQELFK 272
            +EL++
Sbjct: 403 KEELYE 408


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 29.1 bits (62), Expect = 0.68
 Identities = 13/45 (28%), Positives = 26/45 (57%), Gaps = 3/45 (6%)
 Frame = -1

Query: 633 PFPRLHFFIPGFAPLTSRGSQQYRAL---TVPELTQQMFDAKNMM 508
           P PR HF +  + P T++  ++ +A+   TV ++ +++   KN M
Sbjct: 262 PSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQM 306


>SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 390

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 12/39 (30%), Positives = 17/39 (43%)
 Frame = -3

Query: 484 RYLTVAAVFRGRMSMKEVDEQMMNIQNKNSSYFVEWIPN 368
           +Y+   +VF G +          NI N   S   EW+PN
Sbjct: 29  QYIPTISVFEGSLIDNRDTLSYFNISNLEPSERSEWLPN 67


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,430,638
Number of Sequences: 5004
Number of extensions: 42087
Number of successful extensions: 127
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 125
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 345237368
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)