Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= tesV0301.Seq
         (698 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo...    52   6e-08
SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces p...    45   9e-06
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M...    35   0.010
SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar...    31   0.16 
SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces p...    30   0.37 
SPBC776.04 |sec2302|sec23-b|GTPase activating protein Sec23b |Sc...    29   0.85 
SPBC1778.04 |spo6||Spo4-Spo6 kinase complex regulatory subunit S...    27   2.6  
SPAC2F3.15 |lsk1||latrunculin sensitive kinase Lsk1 |Schizosacch...    27   3.4  
SPCC320.09 |hem15||ferrochelatase|Schizosaccharomyces pombe|chr ...    26   4.5  
SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pm...    25   7.9  

>SPBC4.03c |||COPII-coated vesicle component Sfb3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 891

 Score = 52.4 bits (120), Expect = 6e-08
 Identities = 41/143 (28%), Positives = 63/143 (44%)
 Frame = +2

Query: 257 DVGDMFVPILEGFLERVEDSGSVLASLLEQIPAMFRENKETETVMLPAVQAGLEALKAAD 436
           D+ + FVP   G       S  V+  LL+  PA+F++ K  E V+  A++     LK  +
Sbjct: 361 DLENPFVPFSSGLFVDPIASKVVIERLLDSFPAIFQDVKIPEPVVGNALEVVKLLLK--E 418

Query: 437 TAGQLFVFHTTLPTYXRAREARQPSGQEAFGNRPRKSXYWXRRTPRTXXWGAACSVAGVA 616
           T G+ FVF + LPT    +  R    Q  +G    K+  W  +             AG+ 
Sbjct: 419 TGGKAFVFVSALPTMGLGK-LRHREDQRLYGTSDEKN-LWNTQDKWYTSLADEYVTAGIG 476

Query: 617 VQLFACNNAYIXXATWGQLPRLN 685
           V +F    AY+  AT G +  L+
Sbjct: 477 VDIFFTATAYVDVATVGSVATLS 499



 Score = 42.7 bits (96), Expect = 5e-05
 Identities = 24/76 (31%), Positives = 40/76 (52%), Gaps = 1/76 (1%)
 Frame = +3

Query: 33  PKPPATVFVLDVSYNAVKSGLVDAFCENILDII-RELPKDEQGKSYTRVGFITYSSTVHF 209
           PKP   VF++DVSY A   GL     E I +I+   +P D       ++  + +  +V+F
Sbjct: 289 PKPARFVFMIDVSYEASSKGLPKVAAEAIRNILYGPVPLDPN----VKIAIVCFDRSVNF 344

Query: 210 YNIKGSLGQAQMLSVA 257
           +N+  +L Q  ML+ +
Sbjct: 345 FNLSPNLEQPHMLAAS 360


>SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 926

 Score = 45.2 bits (102), Expect = 9e-06
 Identities = 22/75 (29%), Positives = 45/75 (60%)
 Frame = +3

Query: 33  PKPPATVFVLDVSYNAVKSGLVDAFCENILDIIRELPKDEQGKSYTRVGFITYSSTVHFY 212
           P+P   VF++D+S+ ++ SG+V      IL+ +  +P +++G++  +V FI   S +HF+
Sbjct: 327 PQPLVYVFLIDISFVSISSGMVGTASRAILESLDRIP-NKEGRA--KVAFIGVDSALHFF 383

Query: 213 NIKGSLGQAQMLSVA 257
           ++     +A  L V+
Sbjct: 384 SVSPGAEEATQLVVS 398



 Score = 34.7 bits (76), Expect = 0.013
 Identities = 31/145 (21%), Positives = 64/145 (44%), Gaps = 1/145 (0%)
 Frame = +2

Query: 248 VGGDVGDMFVPILEGFLERVEDSGSVLASLLEQIPAMFRENKETETVMLPAVQAGLEALK 427
           V  D+ + F+P  +  L  + +    + +LLE+  +MF   +++   + P ++A    ++
Sbjct: 396 VVSDLEEPFLPRNQDLLLNLRECRQGIENLLERFQSMFATTRDSSNALGPGLKAAHRLIE 455

Query: 428 AADTAGQLFVFHTTLPTYXRAR-EARQPSGQEAFGNRPRKSXYWXRRTPRTXXWGAACSV 604
             +  G++    ++LP     + E R+    +  G   R+S            +   CS 
Sbjct: 456 --NIGGKICCLISSLPNVGVGKLELRE--DPKLLGTN-RESSLLHPNDSFYKSFAVECST 510

Query: 605 AGVAVQLFACNNAYIXXATWGQLPR 679
           + V+V +F  ++ Y   AT   LPR
Sbjct: 511 SQVSVDMFLFSSQYQDVATLSCLPR 535


>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 500

 Score = 35.1 bits (77), Expect = 0.010
 Identities = 22/57 (38%), Positives = 31/57 (54%), Gaps = 2/57 (3%)
 Frame = +3

Query: 30  LPKPPATVFVLDVSYNAVKSGLVDAF--CENILDIIRELPKDEQGKSYTRVGFITYS 194
           L +P  TVFV ++S+NA +  L  AF  C +I  I   LP D Q       G++T+S
Sbjct: 361 LSEPSDTVFVGNLSFNATEDDLSTAFGGCGDIQSI--RLPTDPQSGRLKGFGYVTFS 415


>SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3
            |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1131

 Score = 31.1 bits (67), Expect = 0.16
 Identities = 22/54 (40%), Positives = 28/54 (51%), Gaps = 4/54 (7%)
 Frame = -3

Query: 393  SMTVSVSLFSLNMAGICSSREASTEP----LSSTLSRNPSRMGTNMSPTSPPTA 244
            S+T SVS  S  +A   SS   STEP     SSTLS + S + TN     P ++
Sbjct: 931  SLTSSVSTASSYIASSASSNTLSTEPKTFSSSSTLSESISSINTNSLTVKPESS 984


>SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1040

 Score = 29.9 bits (64), Expect = 0.37
 Identities = 16/62 (25%), Positives = 31/62 (50%), Gaps = 1/62 (1%)
 Frame = -2

Query: 472  QRSVEHEQLTGGVGSFQSFQTSLNGRQHDSLRLLVLSEHGGNL-LQQGGEHRAAVLDPLQ 296
            Q  +++   TGG+ + +  Q  +N     ++R+L++S   GN+ L     +   +LDP  
Sbjct: 905  QEGIKYLMYTGGLSTAERNQALINFEVDPNVRVLLISLKAGNVGLNLTCANHVIILDPFW 964

Query: 295  EP 290
             P
Sbjct: 965  NP 966


>SPBC776.04 |sec2302|sec23-b|GTPase activating protein Sec23b
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 765

 Score = 28.7 bits (61), Expect = 0.85
 Identities = 21/72 (29%), Positives = 32/72 (44%)
 Frame = +3

Query: 3   TTEYCRNNTLPKPPATVFVLDVSYNAVKSGLVDAFCENILDIIRELPKDEQGKSYTRVGF 182
           T EY  +  +  PP  +FV+D    AV    + A  + ++  +  LP D        VG 
Sbjct: 110 TMEYVLSKPVKSPPVFLFVMD---TAVDESELTALKDAVIVSLSLLPPD------AIVGL 160

Query: 183 ITYSSTVHFYNI 218
           ITY S +  + I
Sbjct: 161 ITYGSLIQVHEI 172


>SPBC1778.04 |spo6||Spo4-Spo6 kinase complex regulatory subunit
           Spo6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 474

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 14/51 (27%), Positives = 24/51 (47%), Gaps = 1/51 (1%)
 Frame = +3

Query: 24  NTLPKPPATVFVLD-VSYNAVKSGLVDAFCENILDIIRELPKDEQGKSYTR 173
           N  P+ P   F  D +++  VK      +CEN  +  ++L +   GK + R
Sbjct: 399 NQSPRQPPNAFDADPLAHKKVKIETKSGYCENCCERYKDLERHLGGKHHRR 449


>SPAC2F3.15 |lsk1||latrunculin sensitive kinase Lsk1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 593

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 12/33 (36%), Positives = 19/33 (57%)
 Frame = -3

Query: 342 SSREASTEPLSSTLSRNPSRMGTNMSPTSPPTA 244
           SS  AS  P+S + +RN S+   +    SPP++
Sbjct: 139 SSSSASVSPISPSANRNDSKRQASSFRRSPPSS 171


>SPCC320.09 |hem15||ferrochelatase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 384

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 9/23 (39%), Positives = 17/23 (73%)
 Frame = +3

Query: 81  VKSGLVDAFCENILDIIRELPKD 149
           ++ GL++AF ENI + ++  P+D
Sbjct: 194 LQQGLINAFAENIEETLKTYPED 216


>SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pmc1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1292

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 12/23 (52%), Positives = 15/23 (65%)
 Frame = -3

Query: 342 SSREASTEPLSSTLSRNPSRMGT 274
           + RE S+   SST+SR PS  GT
Sbjct: 38  NQRENSSRSGSSTISREPSSSGT 60


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,233,670
Number of Sequences: 5004
Number of extensions: 40886
Number of successful extensions: 168
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)