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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= tesV0256.Seq
         (598 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyc...    45   1e-05
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa...    42   9e-05
SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy...    38   8e-04
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||...    38   0.001
SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc...    35   0.008
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig...    31   0.097
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo...    31   0.17 
SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces ...    26   4.8  
SPBC18H10.17c |||mitochondrial ribosomal protein subunit Mrp49 |...    25   8.4  

>SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 807

 Score = 44.8 bits (101), Expect = 1e-05
 Identities = 21/50 (42%), Positives = 27/50 (54%)
 Frame = -2

Query: 387 LEXKRKLLQFTTGSDRVPVGGLCNLNFVIAXNGPDCDXLPTAHTSSMSYC 238
           L+ ++  L F TGSDR+P  G  N    I+  GPD D LP +HT     C
Sbjct: 731 LKMQKLFLIFVTGSDRIPATGAHNFQLRISVLGPDSDQLPISHTCFNHLC 780



 Score = 28.3 bits (60), Expect = 0.90
 Identities = 15/39 (38%), Positives = 23/39 (58%)
 Frame = -1

Query: 277 PASHRAHFFNVLLLPEYDTRDKLQDRLLKAINYSKGFGL 161
           P SH    FN L + EY +R+KL+ +L  A+  + GF +
Sbjct: 770 PISHTC--FNHLCIWEYSSREKLKKKLDTALLETNGFNI 806


>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1029

 Score = 41.5 bits (93), Expect = 9e-05
 Identities = 29/110 (26%), Positives = 44/110 (40%), Gaps = 2/110 (1%)
 Frame = -1

Query: 487  DFHELEKSTEYDGAYASDSXMLQXFWGIVXQLXXXXXXXXASVHDRVG-PGARG-RAVQL 314
            D  +L + T Y G Y  +S  +  FW ++ +               V  P   G +A+  
Sbjct: 918  DIDDLRRHTVYAGGYEPNSPTIVLFWEVLREFEEEDKRSFVKFVTSVARPPILGFKALMP 977

Query: 313  ELCDREXRPGLRPASHRAHFFNVLLLPEYDTRDKLQDRLLKAINYSKGFG 164
              C R            +   N+L LP Y T+  L+D+LL A+    GFG
Sbjct: 978  SFCIRVNGEDETRLPTASTCVNLLKLPMYSTKQTLRDKLLTAVRSGVGFG 1027


>SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 786

 Score = 38.3 bits (85), Expect = 8e-04
 Identities = 30/82 (36%), Positives = 37/82 (45%), Gaps = 9/82 (10%)
 Frame = -2

Query: 474 WKSRQN--TMARTRPTLKCYXTFGASXHSLPLEXKRKLLQFTTGSDRVPVGGLCNL---- 313
           WKS     T   T P +K +    A   +   E + KLLQF TG+ R+PV G  +L    
Sbjct: 676 WKSHTEYRTYIATDPVIKWFWEIIAGWKN---EDRSKLLQFATGTSRIPVNGFRDLQGSD 732

Query: 312 ---NFVIAXNGPDCDXLPTAHT 256
               F I   G   D LP AHT
Sbjct: 733 GPRKFTIEKAGTP-DQLPVAHT 753



 Score = 32.7 bits (71), Expect = 0.042
 Identities = 16/47 (34%), Positives = 26/47 (55%)
 Frame = -1

Query: 304 DREXRPGLRPASHRAHFFNVLLLPEYDTRDKLQDRLLKAINYSKGFG 164
           ++   P   P +H    FN L LP+Y ++D L ++L  A+  + GFG
Sbjct: 740 EKAGTPDQLPVAHTC--FNRLDLPDYPSKDTLHEKLSLAVENTVGFG 784


>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 3227

 Score = 37.9 bits (84), Expect = 0.001
 Identities = 22/39 (56%), Positives = 26/39 (66%), Gaps = 1/39 (2%)
 Frame = -1

Query: 277  PASHRAHFFNVLLLPEYDTRDKLQDRLLKAINY-SKGFG 164
            P SH    FN L LPEYDT ++L+  LL AIN  S+GFG
Sbjct: 3189 PQSHTC--FNQLDLPEYDTYEQLRSMLLTAINEGSEGFG 3225



 Score = 30.3 bits (65), Expect = 0.22
 Identities = 14/34 (41%), Positives = 19/34 (55%)
 Frame = -2

Query: 414  FGASXHSLPLEXKRKLLQFTTGSDRVPVGGLCNL 313
            F  +  S   E + KLLQF TG+ +VP+ G   L
Sbjct: 3134 FWRAVRSFDEEERAKLLQFATGTSKVPLNGFKEL 3167


>SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 767

 Score = 35.1 bits (77), Expect = 0.008
 Identities = 22/50 (44%), Positives = 28/50 (56%), Gaps = 7/50 (14%)
 Frame = -2

Query: 384 EXKRKLLQFTTGSDRVPVGGLCNL-------NFVIAXNGPDCDXLPTAHT 256
           E K +LLQFTTG+ R+PV G  +L        F I   G + + LP AHT
Sbjct: 686 EKKSRLLQFTTGTSRIPVNGFKDLQGSDGPRKFTIEKAG-EPNKLPKAHT 734



 Score = 27.1 bits (57), Expect = 2.1
 Identities = 15/47 (31%), Positives = 23/47 (48%)
 Frame = -1

Query: 304 DREXRPGLRPASHRAHFFNVLLLPEYDTRDKLQDRLLKAINYSKGFG 164
           ++   P   P +H    FN L LP Y ++  L  +L  A+  + GFG
Sbjct: 721 EKAGEPNKLPKAHTC--FNRLDLPPYTSKKDLDHKLSIAVEETIGFG 765


>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
            E3 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1647

 Score = 31.5 bits (68), Expect = 0.097
 Identities = 12/25 (48%), Positives = 17/25 (68%)
 Frame = -2

Query: 384  EXKRKLLQFTTGSDRVPVGGLCNLN 310
            + +R  LQF TGS ++P+GG   LN
Sbjct: 1564 QEQRDFLQFITGSRKLPIGGFAGLN 1588



 Score = 25.8 bits (54), Expect = 4.8
 Identities = 13/27 (48%), Positives = 16/27 (59%)
 Frame = -1

Query: 250  NVLLLPEYDTRDKLQDRLLKAINYSKG 170
            N L LPEY + + L  RL KAI   +G
Sbjct: 1616 NYLKLPEYSSSEVLGSRLSKAILEGQG 1642


>SPAC1805.15c |pub2||ubiquitin-protein ligase
           Pub2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 671

 Score = 30.7 bits (66), Expect = 0.17
 Identities = 20/50 (40%), Positives = 26/50 (52%), Gaps = 7/50 (14%)
 Frame = -2

Query: 384 EXKRKLLQFTTGSDRVPVGGLCNLN-------FVIAXNGPDCDXLPTAHT 256
           E K KLLQF TG+ R+P+ G  +++       F I   G     LP AHT
Sbjct: 590 EKKAKLLQFATGTSRLPLSGFKDMHGSDGPRKFTIEKVG-HISQLPKAHT 638



 Score = 29.9 bits (64), Expect = 0.29
 Identities = 14/35 (40%), Positives = 24/35 (68%), Gaps = 1/35 (2%)
 Frame = -1

Query: 265 RAHF-FNVLLLPEYDTRDKLQDRLLKAINYSKGFG 164
           +AH  FN L +P Y+++++L+ +L  AI  + GFG
Sbjct: 635 KAHTCFNRLDIPPYNSKEELEQKLTIAIQETAGFG 669


>SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 582

 Score = 25.8 bits (54), Expect = 4.8
 Identities = 12/52 (23%), Positives = 21/52 (40%)
 Frame = -2

Query: 576 FLEGXRLRIPLGTFFSPERS*NAVCGSRFSISTSWKSRQNTMARTRPTLKCY 421
           F     LR  + +  + E+    +CG RFS   + +  +       P L C+
Sbjct: 432 FKRSEHLRRHIRSLHTSEKPFVCICGKRFSRRDNLRQHERLHVNASPRLACF 483


>SPBC18H10.17c |||mitochondrial ribosomal protein subunit Mrp49
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 175

 Score = 25.0 bits (52), Expect = 8.4
 Identities = 12/38 (31%), Positives = 18/38 (47%)
 Frame = -1

Query: 286 GLRPASHRAHFFNVLLLPEYDTRDKLQDRLLKAINYSK 173
           G+RPA  R   +  LL  E+      Q   ++ +NY K
Sbjct: 47  GMRPAKLRKDHWVPLLKVEFPNESMFQSVFMQLLNYRK 84


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,993,805
Number of Sequences: 5004
Number of extensions: 32714
Number of successful extensions: 102
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 92
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 101
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 260219058
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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