Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesV0004.Seq
(698 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal ident... 66 2e-11
At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal, puta... 61 8e-10
At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast... 55 5e-08
At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondri... 54 9e-08
At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondri... 49 3e-06
At3g48850.1 68416.m05335 mitochondrial phosphate transporter, pu... 31 0.97
At1g02170.1 68414.m00145 latex-abundant family protein (AMC1) / ... 27 9.0
>At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal
identical to SP|Q9ZP05; identical to cDNA microbody
NAD-dependent malate dehydrogenase GI:3929650
Length = 354
Score = 66.5 bits (155), Expect = 2e-11
Identities = 34/83 (40%), Positives = 46/83 (55%)
Frame = +3
Query: 3 IASEVLK*AGXYDPNRXXXXXXXXXXXXXXXXXEXXGVDPTSVAVPVIGGHSGITIIPIL 182
IA+EV K AG YDP + E G+DP V VPV+GGH+G+TI+P+L
Sbjct: 168 IAAEVFKKAGTYDPKKLLGVTTLDVARANTFVAEVLGLDPREVDVPVVGGHAGVTILPLL 227
Query: 183 XQCQPAXKXSDPAKIKAXTERIQ 251
Q +P + P +I+ T RIQ
Sbjct: 228 SQVKPPSSFT-PQEIEYLTNRIQ 249
Score = 44.0 bits (99), Expect = 1e-04
Identities = 20/30 (66%), Positives = 25/30 (83%)
Frame = +1
Query: 289 AGSATLSMAYAGARLTASVLRGLKGESNVV 378
AGSATLSMAYA A+ + LRGL+G++NVV
Sbjct: 262 AGSATLSMAYAAAKFADACLRGLRGDANVV 291
>At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal,
putative strong similarity to glyoxysomal malate
dehydrogenase (EC 1.1.1.37) SP|P19446 {Citrullus
lanatus}, SP|P46488 {Cucumis sativus}, [Medicago sativa]
GI:2827078, SP|Q42972 {Oryza sativa}, SP|Q9ZP05
{Arabidopsis thaliana}, SP|P37228 {Glycine max};
contains InterPro entry IPR001236: Lactate/malate
dehydrogenase
Length = 354
Score = 60.9 bits (141), Expect = 8e-10
Identities = 31/83 (37%), Positives = 45/83 (54%)
Frame = +3
Query: 3 IASEVLK*AGXYDPNRXXXXXXXXXXXXXXXXXEXXGVDPTSVAVPVIGGHSGITIIPIL 182
IA+EV K AG +DP + E +DP V VPV+GGH+G+TI+P+L
Sbjct: 168 IAAEVFKKAGTFDPKKLMGVTMLDVVRANTFVAEVMSLDPREVEVPVVGGHAGVTILPLL 227
Query: 183 XQCQPAXKXSDPAKIKAXTERIQ 251
Q +P + +I+ T+RIQ
Sbjct: 228 SQVKPPCSFTQ-KEIEYLTDRIQ 249
Score = 41.1 bits (92), Expect = 7e-04
Identities = 18/30 (60%), Positives = 23/30 (76%)
Frame = +1
Query: 289 AGSATLSMAYAGARLTASVLRGLKGESNVV 378
AGSATLSMAYA + LRGL+G++N+V
Sbjct: 262 AGSATLSMAYAAVEFADACLRGLRGDANIV 291
>At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast
(MDH) identical to chloroplast NAD-malate dehydrogenase
[Arabidopsis thaliana] GI:3256066; contains InterPro
entry IPR001236: Lactate/malate dehydrogenase; contains
Pfam profiles PF00056: lactate/malate dehydrogenase, NAD
binding domain and PF02866: lactate/malate
dehydrogenase, alpha/beta C-terminal domain
Length = 403
Score = 54.8 bits (126), Expect = 5e-08
Identities = 31/83 (37%), Positives = 44/83 (53%)
Frame = +3
Query: 3 IASEVLK*AGXYDPNRXXXXXXXXXXXXXXXXXEXXGVDPTSVAVPVIGGHSGITIIPIL 182
IA+EVLK G YDP + + + V VPVIGGH+GITI+P+L
Sbjct: 208 IAAEVLKKKGVYDPKKLFGVTTLDVVRANTFVSQKKNLKLIDVDVPVIGGHAGITILPLL 267
Query: 183 XQCQPAXKXSDPAKIKAXTERIQ 251
+ +P+ +D +I+ T RIQ
Sbjct: 268 SKTKPSVNFTD-EEIQELTVRIQ 289
Score = 38.7 bits (86), Expect = 0.004
Identities = 19/29 (65%), Positives = 21/29 (72%)
Frame = +1
Query: 289 AGSATLSMAYAGARLTASVLRGLKGESNV 375
AGSATLSMAYA AR S LR L G+ +V
Sbjct: 302 AGSATLSMAYAAARFVESSLRALDGDGDV 330
>At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondrial,
putative similar to mitochondrial NAD-dependent malate
dehydrogenase GB:CAA10320 SP|Q9ZP06 [Arabidopsis
thaliana]; contains InterPro entry IPR001236:
Lactate/malate dehydrogenase
Length = 341
Score = 54.0 bits (124), Expect = 9e-08
Identities = 30/84 (35%), Positives = 41/84 (48%)
Frame = +3
Query: 3 IASEVLK*AGXYDPNRXXXXXXXXXXXXXXXXXEXXGVDPTSVAVPVIGGHSGITIIPIL 182
IA+E+ K AG YD + V+ V VPV+GGH+GITI+P+
Sbjct: 155 IAAEIFKKAGTYDEKKLFGVTTLDVVRARTFYAGKSDVNVAEVNVPVVGGHAGITILPLF 214
Query: 183 XQCQPAXKXSDPAKIKAXTERIQE 254
Q P SD I+A T+R Q+
Sbjct: 215 SQASPQANLSDDL-IRALTKRTQD 237
Score = 38.7 bits (86), Expect = 0.004
Identities = 19/29 (65%), Positives = 21/29 (72%)
Frame = +1
Query: 292 GSATLSMAYAGARLTASVLRGLKGESNVV 378
GSATLSMAYAGA + L+GL G NVV
Sbjct: 250 GSATLSMAYAGALFADACLKGLNGVPNVV 278
>At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondrial
identical to mitochondrial NAD-dependent malate
dehydrogenase GI:3929649 SP|Q9ZP06 from [Arabidopsis
thaliana]; contains InterPro entry IPR001236:
Lactate/malate dehydrogenase
Length = 341
Score = 48.8 bits (111), Expect = 3e-06
Identities = 28/84 (33%), Positives = 38/84 (45%)
Frame = +3
Query: 3 IASEVLK*AGXYDPNRXXXXXXXXXXXXXXXXXEXXGVDPTSVAVPVIGGHSGITIIPIL 182
IA+E+ K AG YD + V V VPVIGGH+G+TI+P+
Sbjct: 155 IAAEIFKKAGMYDEKKLFGVTTLDVVRARTFYAGKANVPVAEVNVPVIGGHAGVTILPLF 214
Query: 183 XQCQPAXKXSDPAKIKAXTERIQE 254
Q P S + A T+R Q+
Sbjct: 215 SQATPQANLSSDI-LTALTKRTQD 237
Score = 36.3 bits (80), Expect = 0.020
Identities = 17/29 (58%), Positives = 21/29 (72%)
Frame = +1
Query: 292 GSATLSMAYAGARLTASVLRGLKGESNVV 378
GSATLSMAYAGA + L+GL G +V+
Sbjct: 250 GSATLSMAYAGALFADACLKGLNGVPDVI 278
>At3g48850.1 68416.m05335 mitochondrial phosphate transporter,
putative similar to mitochondrial phosphate transporter
GI:3318617 from [Arabidopsis thaliana]
Length = 363
Score = 30.7 bits (66), Expect = 0.97
Identities = 16/45 (35%), Positives = 25/45 (55%), Gaps = 3/45 (6%)
Frame = -2
Query: 142 TGTATEVGSTPXISPTKVAVA---TTSRVVTPSTRFGSYXPAYLS 17
T +T + S P ISPT +V+ T+ + TP+ + Y PAY +
Sbjct: 24 TTMSTHLKSQPLISPTNSSVSSNGTSFAIATPNEKVEMYSPAYFA 68
>At1g02170.1 68414.m00145 latex-abundant family protein (AMC1) /
caspase family protein contains similarity to
latex-abundant protein [Hevea brasiliensis] gb:AAD13216;
contains Pfam profile PF00656: ICE-like protease
(caspase) p20 domain
Length = 367
Score = 27.5 bits (58), Expect = 9.0
Identities = 21/74 (28%), Positives = 28/74 (37%)
Frame = +2
Query: 338 PPCSGX*RASPTWFXCP*VKSXLXXAT*FRQXRCARQERPPEGSXVRDPSTNXXAASPEX 517
PP A P C ++ L + R RCA + + + DP T P
Sbjct: 3 PPPPSSIYAPPMLVNCSGCRTPLQLPSGARSIRCALCQAV---THIADPRT-APPPQPSS 58
Query: 518 GPRPTXQEHSRPGQ 559
P P Q H+ PGQ
Sbjct: 59 APSPPPQIHAPPGQ 72
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,124,174
Number of Sequences: 28952
Number of extensions: 142195
Number of successful extensions: 327
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 303
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 325
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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