BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesS1253
(499 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g10350.1 68417.m01700 no apical meristem (NAM) family protein... 34 0.046
At5g63540.1 68418.m07975 expressed protein ; expression support... 31 0.43
At5g26130.1 68418.m03108 pathogenesis-related protein, putative ... 31 0.43
At2g32140.1 68415.m03928 disease resistance protein (TIR class),... 31 0.57
At1g31370.1 68414.m03839 expressed protein ; expression supporte... 29 1.3
At3g62110.1 68416.m06978 glycoside hydrolase family 28 protein /... 29 2.3
At4g29730.1 68417.m04233 WD-40 repeat family protein contains 5 ... 28 3.0
At5g08010.1 68418.m00932 expressed protein condensin subunit SMC... 28 4.0
At4g16390.1 68417.m02481 chloroplastic RNA-binding protein P67, ... 28 4.0
At4g03430.1 68417.m00470 pre-mRNA splicing factor-related simila... 28 4.0
At2g18730.1 68415.m02181 diacylglycerol kinase, putative contain... 27 7.0
At1g60770.1 68414.m06841 pentatricopeptide (PPR) repeat-containi... 27 7.0
At5g19560.1 68418.m02329 hypothetical protein contains Pfam prof... 27 9.3
At1g03930.1 68414.m00378 protein kinase (ADK1) identical to dual... 27 9.3
>At4g10350.1 68417.m01700 no apical meristem (NAM) family protein
contains Pfam PF02365: No apical meristem (NAM) domain;
nap gene, Arabidopsis thaliana, gb:AJ222713
Length = 341
Score = 34.3 bits (75), Expect = 0.046
Identities = 15/44 (34%), Positives = 24/44 (54%)
Frame = -2
Query: 303 GASLGNGDSVTSNAHRILIWVWRLTDHLTTASNGSDSSSRGTEY 172
G +G ++V S+ H+ + W + D L T G++ SSRG Y
Sbjct: 265 GLDVGTCETVASHNHQQGLGEWAMMDRLVTCHMGNEDSSRGITY 308
>At5g63540.1 68418.m07975 expressed protein ; expression supported
by MPSS
Length = 602
Score = 31.1 bits (67), Expect = 0.43
Identities = 31/108 (28%), Positives = 46/108 (42%)
Frame = -2
Query: 492 ISVYLTRKTKNTVRTASYNIKRPVADISQHPNHDSEQPPELLKTAVPRRGAKLNARSTSI 313
+ ++R + TAS + +R +DI + + + T GA ARS +
Sbjct: 318 VGATVSRVEHMQIDTASAHGERTFSDIHS-TSSNIHRAASTAGTGTSCSGACSGARSFAN 376
Query: 312 LVRGASLGNGDSVTSNAHRILIWVWRLTDHLTTASNGSDSSSRGTEYS 169
V G SL +VTS + I R+ D TT GSDS E+S
Sbjct: 377 NVGGNSLDQTSNVTSFVEEMHIDTGRVRD-TTTHIYGSDSGGVAAEFS 423
>At5g26130.1 68418.m03108 pathogenesis-related protein, putative
similar to PR-1a protein [Nicotiana tabacum] GI:19944;
contains Pfam profile PF00188: SCP-like extracellular
protein
Length = 164
Score = 31.1 bits (67), Expect = 0.43
Identities = 22/85 (25%), Positives = 35/85 (41%)
Frame = -2
Query: 360 AVPRRGAKLNARSTSILVRGASLGNGDSVTSNAHRILIWVWRLTDHLTTASNGSDSSSRG 181
A R+G S S + G +L S A + +WV +D++ ++ SD G
Sbjct: 62 AQQRKGDCSLTHSNSNGLYGENLAWSGGALSGAEAVKLWVNEKSDYIYASNTCSDGKQCG 121
Query: 180 TEYSTTCRTARRAYSKARMACDTGG 106
RT+ A++ CD GG
Sbjct: 122 HYTQVVWRTSEWV-GCAKVKCDNGG 145
>At2g32140.1 68415.m03928 disease resistance protein (TIR class),
putative domain signature TIR exists, suggestive of a
disease resistance protein.
Length = 371
Score = 30.7 bits (66), Expect = 0.57
Identities = 36/125 (28%), Positives = 51/125 (40%), Gaps = 3/125 (2%)
Frame = -2
Query: 462 NTVRTASYNIKRPVADI-SQHPNHDSEQPP--ELLKTAVPRRGAKLNARSTSILVRGASL 292
+T+R S N V SQ PN D E LLK V AKL A +TSI+ A L
Sbjct: 242 STMRKVSSNNGVGVGTFKSQKPNFDDEDDDGDSLLKALV----AKLVASTTSIVAAYAEL 297
Query: 291 GNGDSVTSNAHRILIWVWRLTDHLTTASNGSDSSSRGTEYSTTCRTARRAYSKARMACDT 112
S A ++ + L +SNG S S+ RR+ + ++ +
Sbjct: 298 QRAHSDAIQAAETVVVDVKTLSELIRSSNGGGGGSGSGSGSSLENQPRRSKVRKQVWAEV 357
Query: 111 GGKAS 97
G +S
Sbjct: 358 LGISS 362
>At1g31370.1 68414.m03839 expressed protein ; expression supported
by MPSS
Length = 193
Score = 29.5 bits (63), Expect = 1.3
Identities = 16/38 (42%), Positives = 21/38 (55%)
Frame = -3
Query: 464 KTPFEQRVTILSGQSPTFPNIRTTTANSLQSS*RPRCH 351
K+ FE+ I S P P++RTT+ NSL S CH
Sbjct: 126 KSLFEKHPDIASKFRPKNPHLRTTSLNSLLSLTEILCH 163
>At3g62110.1 68416.m06978 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein weak
similarity to polygalacturonase [Lycopersicon
esculentum] GI:4325090; contains PF00295: Glycosyl
hydrolases family 28
Length = 471
Score = 28.7 bits (61), Expect = 2.3
Identities = 17/58 (29%), Positives = 26/58 (44%)
Frame = -2
Query: 297 SLGNGDSVTSNAHRILIWVWRLTDHLTTASNGSDSSSRGTEYSTTCRTARRAYSKARM 124
S G G + HR LI+ LTD + T NG+ +GT + R Y++ +
Sbjct: 125 SYGRGRELPGRRHRSLIYGQNLTDVVITGENGT-IDGQGTVWWDWFRNGELNYTRPHL 181
>At4g29730.1 68417.m04233 WD-40 repeat family protein contains 5
WD-40 repeats (PF0400); similar to WD-40 repeat protein
MSI4 (SP:O22607) [Arabidopsis thaliana]
Length = 496
Score = 28.3 bits (60), Expect = 3.0
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = -2
Query: 243 VWRLTDHLTTASNGSDSSSRGTEYSTT 163
+W + DH+T A GSDS S G+ + T
Sbjct: 235 LWNIQDHITMA--GSDSKSPGSSFKQT 259
>At5g08010.1 68418.m00932 expressed protein condensin subunit SMC4,
Drosophila melanogaster, EMBL:AF186472
Length = 566
Score = 27.9 bits (59), Expect = 4.0
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +1
Query: 37 SIPKFHSSLQDLKQPSLQEPRCLATRV 117
++ K SS +DL L+ PRCLA+ V
Sbjct: 21 NVSKGKSSSEDLTNVKLESPRCLASNV 47
>At4g16390.1 68417.m02481 chloroplastic RNA-binding protein P67,
putative nearly identical to 67kD chloroplastic
RNA-binding protein, P67 [Arabidopsis thaliana]
GI:9755842
Length = 688
Score = 27.9 bits (59), Expect = 4.0
Identities = 13/44 (29%), Positives = 24/44 (54%), Gaps = 4/44 (9%)
Frame = +1
Query: 307 DEYGRRARVELRSASWHRGLQELWRL----FAVVVRMLGNVGDW 426
D YGR V++ + + R E WR+ F+ ++R+ G G++
Sbjct: 239 DAYGRAGNVDMALSLYDRARTEKWRIDAVTFSTLIRIYGVSGNY 282
>At4g03430.1 68417.m00470 pre-mRNA splicing factor-related similar
to pre-mRNA splicing factor pre-mRNA splicing factor
prp1 (SP:Q12381) [Fission yeast]
Length = 1029
Score = 27.9 bits (59), Expect = 4.0
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = +1
Query: 322 RARVELRSASWHRGLQELWRLFAVVVRMLGNV 417
RAR+ L A G + +W A+V R LGNV
Sbjct: 745 RARMLLAKARERGGTERVWMKSAIVERELGNV 776
>At2g18730.1 68415.m02181 diacylglycerol kinase, putative contains
INTERPRO domain, IPR001206, DAG-kinase catalytic domain
Length = 488
Score = 27.1 bits (57), Expect = 7.0
Identities = 15/51 (29%), Positives = 26/51 (50%), Gaps = 3/51 (5%)
Frame = +2
Query: 296 DAPLTSMDVERALSFAPRRGTAVFKSSGGC---SLSWFGCWEMSATGRLIL 439
D+P++ D + A R TA K+ GC +L+W G ++ RL++
Sbjct: 2 DSPVSKTDASKEKFVASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMM 52
>At1g60770.1 68414.m06841 pentatricopeptide (PPR) repeat-containing
protein low similarity to DNA-binding protein [Triticum
aestivum] GI:6958202; contains Pfam profile PF01535: PPR
repeat
Length = 491
Score = 27.1 bits (57), Expect = 7.0
Identities = 15/47 (31%), Positives = 23/47 (48%)
Frame = -2
Query: 447 ASYNIKRPVADISQHPNHDSEQPPELLKTAVPRRGAKLNARSTSILV 307
++Y+I+ I + Q LK PRRG KLNA++ I +
Sbjct: 330 STYDIRIVNVLIGAYAQEGLIQKANELKEKAPRRGGKLNAKTWEIFM 376
>At5g19560.1 68418.m02329 hypothetical protein contains Pfam profile
PF03759: Domain of unknown function (DUF315)
Length = 493
Score = 26.6 bits (56), Expect = 9.3
Identities = 13/39 (33%), Positives = 21/39 (53%)
Frame = +1
Query: 19 KSSGTSSIPKFHSSLQDLKQPSLQEPRCLATRVTGHARL 135
K S S I FH Q+ ++P ++ PR L + + A+L
Sbjct: 445 KHSSMSDIEFFHKVEQEKEKPMMKSPRALPKKFSYLAKL 483
>At1g03930.1 68414.m00378 protein kinase (ADK1) identical to dual
specificity kinase 1 (ADK1) [Arabidopsis thaliana]
gi|1216484|gb|AAB47968; supported by cDNA gi:18700076
and gi:1216483. Note: differences between cDNAs in the
11th exon, possibly due to errors or alternative
splicing.
Length = 471
Score = 26.6 bits (56), Expect = 9.3
Identities = 16/38 (42%), Positives = 22/38 (57%)
Frame = -1
Query: 487 RIFNTENEKHRSNSELQY*AASRRHFPTSEPRQRTASR 374
RI E ++R + ++ A SRRH TS R R+ASR
Sbjct: 329 RIAGKETRENRFSGAVE--AFSRRHPATSTTRDRSASR 364
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,350,722
Number of Sequences: 28952
Number of extensions: 183078
Number of successful extensions: 632
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 618
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 631
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 878448512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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