Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= tesS1226
         (608 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   120   1e-28
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...    72   6e-14
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...    71   1e-13
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    66   3e-12
SPBC19G7.16 |iws1||transcription elongation factor complex subun...    27   2.1  
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    27   2.1  
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos...    27   2.8  
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz...    25   6.5  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  120 bits (290), Expect = 1e-28
 Identities = 66/166 (39%), Positives = 78/166 (46%)
 Frame = +1

Query: 67  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 246
           MREIVHIQAGQCGNQ+GA FW  I+DEHG+D  G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60

Query: 247 PRASSSTWSPAPWTLSALDLXXXXXXXXXXXXXXXXXXXXGPRDTTRRVLSSSIQF*TSX 426
           PRA      P                                +         +       
Sbjct: 61  PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120

Query: 427 RKEAEXCDCLQGFQXXXXXXXXXXXXMAPFXSPKSENKXPDRIMNT 564
           R+EAE CD LQGFQ            M      K   + PDR+M T
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMAT 166



 Score =  113 bits (272), Expect = 2e-26
 Identities = 57/100 (57%), Positives = 68/100 (68%), Gaps = 1/100 (1%)
 Frame = +3

Query: 249 PR-ILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVR 425
           PR +LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDV 
Sbjct: 61  PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120

Query: 426 SKGSGXV*LPPGIPTDTLARXRHXFRYGTLLISKIREQVP 545
            + +       G               GTLL+SKIRE+ P
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYP 160


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score = 72.1 bits (169), Expect = 6e-14
 Identities = 31/58 (53%), Positives = 42/58 (72%), Gaps = 1/58 (1%)
 Frame = +3

Query: 249 PR-ILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 419
           PR I VDLEP  +D VR+GP+  +F P+  V G+  A NN+A+GHYT G E++DSVL+
Sbjct: 67  PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLE 124



 Score = 49.6 bits (113), Expect = 4e-07
 Identities = 24/69 (34%), Positives = 36/69 (52%), Gaps = 6/69 (8%)
 Frame = +1

Query: 67  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 228
           MRE++ +  GQ G QIG   WE+   EHGI P G        H ++    +    +++E 
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60

Query: 229 SGGKYVPRA 255
             GK+VPR+
Sbjct: 61  GQGKFVPRS 69


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score = 70.9 bits (166), Expect = 1e-13
 Identities = 31/58 (53%), Positives = 40/58 (68%), Gaps = 1/58 (1%)
 Frame = +3

Query: 249 PR-ILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 419
           PR I VDLEP  +D VR+GP+  +F P+  + G+  A NN+A+GHYT G ELVD V D
Sbjct: 63  PRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120



 Score = 55.6 bits (128), Expect = 5e-09
 Identities = 27/65 (41%), Positives = 37/65 (56%), Gaps = 2/65 (3%)
 Frame = +1

Query: 67  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 240
           MREI+ I  GQ G QIG   WE+   EHGI P G  + ++  Q      + +++E   GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 241 YVPRA 255
           YVPR+
Sbjct: 61  YVPRS 65


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 66.5 bits (155), Expect = 3e-12
 Identities = 27/62 (43%), Positives = 43/62 (69%)
 Frame = +1

Query: 70  REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 249
           REI+ +QAGQCGNQIG++FW+ +  EHGI P G     +   ++R +V++ ++   +Y+P
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62

Query: 250 RA 255
           RA
Sbjct: 63  RA 64



 Score = 50.4 bits (115), Expect = 2e-07
 Identities = 22/61 (36%), Positives = 40/61 (65%), Gaps = 3/61 (4%)
 Frame = +3

Query: 249 PR-ILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLD 419
           PR IL+DLEP  ++++ S  +G ++ P+N +  ++  GAGNNWA G Y+    + + ++D
Sbjct: 62  PRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMD 120

Query: 420 V 422
           +
Sbjct: 121 M 121


>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
           Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 428

 Score = 27.1 bits (57), Expect = 2.1
 Identities = 10/34 (29%), Positives = 22/34 (64%)
 Frame = +1

Query: 106 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 207
           N++G    E+++++  +DPT A   + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166


>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 800

 Score = 27.1 bits (57), Expect = 2.1
 Identities = 22/66 (33%), Positives = 26/66 (39%)
 Frame = -2

Query: 481 ASVSVGIPGGNHTIPLPFXRTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPKG 302
           +S S     G+ + PLP   TS T STS  P+           P  P T  S   I P  
Sbjct: 93  SSTSSASTTGSSSSPLPSTSTSCTTSTSIPPTGGSSSLSTPITPTVPPTSTSSTSI-PIP 151

Query: 301 PERTES 284
           P  T S
Sbjct: 152 PTSTSS 157


>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
           Pof11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 506

 Score = 26.6 bits (56), Expect = 2.8
 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
 Frame = +3

Query: 300 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 401
           GP+G +F P  F+F  +G    NW+   Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190


>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 255

 Score = 25.4 bits (53), Expect = 6.5
 Identities = 13/32 (40%), Positives = 16/32 (50%)
 Frame = -2

Query: 358 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 263
           P+   PK  L  R I P GPE  +  + GS S
Sbjct: 20  PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,271,665
Number of Sequences: 5004
Number of extensions: 41271
Number of successful extensions: 131
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 115
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 127
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 268287866
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)