Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesS1185
(598 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q17221 Cluster: Nd-s mutant fibroin light chain; n=3; B... 157 2e-37
UniRef50_P21828 Cluster: Fibroin light chain precursor; n=8; Bom... 157 2e-37
UniRef50_Q9BLL7 Cluster: Fibroin L-chain; n=1; Dendrolimus spect... 79 8e-14
UniRef50_Q26427 Cluster: Fibroin light chain precursor; n=2; Obt... 69 1e-10
UniRef50_Q14UU5 Cluster: Light-chain fibroin; n=1; Yponomeuta ev... 58 2e-07
UniRef50_Q9FWL9 Cluster: Putative uncharacterized protein OSJNBa... 38 0.18
UniRef50_UPI0000DA2531 Cluster: PREDICTED: hypothetical protein;... 35 1.3
UniRef50_UPI0000E45C65 Cluster: PREDICTED: hypothetical protein;... 35 1.7
UniRef50_A6RJB3 Cluster: Putative uncharacterized protein; n=1; ... 33 5.1
UniRef50_UPI0000E25565 Cluster: PREDICTED: hypothetical protein;... 33 6.7
UniRef50_A3JVU6 Cluster: Membrane protein; n=1; Rhodobacterales ... 33 6.7
UniRef50_Q0J1I0 Cluster: Os09g0439000 protein; n=5; Magnoliophyt... 33 6.7
UniRef50_Q18Q44 Cluster: TraB family protein; n=3; Firmicutes|Re... 32 8.9
UniRef50_A6BJ85 Cluster: Putative uncharacterized protein; n=1; ... 32 8.9
UniRef50_A5L6F4 Cluster: Probable binding protein component of A... 32 8.9
>UniRef50_Q17221 Cluster: Nd-s mutant fibroin light chain; n=3;
Bombyx mori|Rep: Nd-s mutant fibroin light chain -
Bombyx mori (Silk moth)
Length = 276
Score = 157 bits (380), Expect = 2e-37
Identities = 76/76 (100%), Positives = 76/76 (100%)
Frame = +1
Query: 28 MKPIFLVLLVATSAYAAPSVTINQYSDNEIPRDIDDGKASSVISRAWDYVDDTDKSIAIL 207
MKPIFLVLLVATSAYAAPSVTINQYSDNEIPRDIDDGKASSVISRAWDYVDDTDKSIAIL
Sbjct: 1 MKPIFLVLLVATSAYAAPSVTINQYSDNEIPRDIDDGKASSVISRAWDYVDDTDKSIAIL 60
Query: 208 NVQEILKDMASQGDYA 255
NVQEILKDMASQGDYA
Sbjct: 61 NVQEILKDMASQGDYA 76
Score = 63.7 bits (148), Expect = 3e-09
Identities = 32/44 (72%), Positives = 34/44 (77%)
Frame = +3
Query: 222 LEGHGQPGRLCSQASAVAQTAGIIAHLSAGIPGDACAAANVINS 353
L+ G SQASAVAQTAGIIAHLSAGIPGDACAAAN + S
Sbjct: 66 LKDMASQGDYASQASAVAQTAGIIAHLSAGIPGDACAAANSMGS 109
>UniRef50_P21828 Cluster: Fibroin light chain precursor; n=8;
Bombyx|Rep: Fibroin light chain precursor - Bombyx mori
(Silk moth)
Length = 262
Score = 157 bits (380), Expect = 2e-37
Identities = 76/76 (100%), Positives = 76/76 (100%)
Frame = +1
Query: 28 MKPIFLVLLVATSAYAAPSVTINQYSDNEIPRDIDDGKASSVISRAWDYVDDTDKSIAIL 207
MKPIFLVLLVATSAYAAPSVTINQYSDNEIPRDIDDGKASSVISRAWDYVDDTDKSIAIL
Sbjct: 1 MKPIFLVLLVATSAYAAPSVTINQYSDNEIPRDIDDGKASSVISRAWDYVDDTDKSIAIL 60
Query: 208 NVQEILKDMASQGDYA 255
NVQEILKDMASQGDYA
Sbjct: 61 NVQEILKDMASQGDYA 76
Score = 151 bits (366), Expect = 1e-35
Identities = 74/96 (77%), Positives = 75/96 (78%)
Frame = +3
Query: 222 LEGHGQPGRLCSQASAVAQTAGIIAHLSAGIPGDACAAANVINSYTDGVRSGNFXGFRQS 401
L+ G SQASAVAQTAGIIAHLSAGIPGDACAAANVINSYTDGVRSGNF GFRQS
Sbjct: 66 LKDMASQGDYASQASAVAQTAGIIAHLSAGIPGDACAAANVINSYTDGVRSGNFAGFRQS 125
Query: 402 LGPFFGHVGXXXXXXXXXXXXPGQLRYSVGPALGCA 509
LGPFFGHVG PGQLRYSVGPALGCA
Sbjct: 126 LGPFFGHVGQNLNLINQLVINPGQLRYSVGPALGCA 161
Score = 68.1 bits (159), Expect = 1e-10
Identities = 30/33 (90%), Positives = 30/33 (90%)
Frame = +2
Query: 500 GLCGGGRIYDFEAAWDAILASSDSSFLNXEYCI 598
G GGGRIYDFEAAWDAILASSDSSFLN EYCI
Sbjct: 159 GCAGGGRIYDFEAAWDAILASSDSSFLNEEYCI 191
>UniRef50_Q9BLL7 Cluster: Fibroin L-chain; n=1; Dendrolimus
spectabilis|Rep: Fibroin L-chain - Dendrolimus
spectabilis (pine moth)
Length = 263
Score = 79.0 bits (186), Expect = 8e-14
Identities = 39/85 (45%), Positives = 51/85 (60%)
Frame = +3
Query: 255 SQASAVAQTAGIIAHLSAGIPGDACAAANVINSYTDGVRSGNFXGFRQSLGPFFGHVGXX 434
SQA A+AQT LS+GIPGDACA+A+V N+Y+ VRSGN GFR +L + ++
Sbjct: 80 SQARALAQTIATAIDLSSGIPGDACASADVANAYSAAVRSGNPSGFRSALNRYIKYIASN 139
Query: 435 XXXXXXXXXXPGQLRYSVGPALGCA 509
P RYSVGP+ GC+
Sbjct: 140 LDSIVRIANNPNSGRYSVGPSGGCS 164
Score = 72.5 bits (170), Expect = 7e-12
Identities = 37/76 (48%), Positives = 50/76 (65%), Gaps = 2/76 (2%)
Frame = +1
Query: 28 MKPIFLVLLVATSAYAAPSVTINQYSDNEIPRDIDDGK--ASSVISRAWDYVDDTDKSIA 201
M+PI LVLL ATSA AAPSV + QYS+NE+ D+GK +S + R +D D D +I
Sbjct: 2 MRPIVLVLLFATSALAAPSVLLKQYSENEVAPTKDNGKQVSSYLTDRTFDLFDGGDNNIY 61
Query: 202 ILNVQEILKDMASQGD 249
ILN +++ D A+ GD
Sbjct: 62 ILNAMQLMNDFANSGD 77
Score = 44.0 bits (99), Expect = 0.003
Identities = 22/57 (38%), Positives = 29/57 (50%)
Frame = +2
Query: 428 TKLESYQSTRHQPWSTPILCRTSPGLCGGGRIYDFEAAWDAILASSDSSFLNXEYCI 598
+ L+S + P S S G GGGR YDFE+ W ++LA S SS YC+
Sbjct: 138 SNLDSIVRIANNPNSGRYSVGPSGGCSGGGRSYDFESVWQSVLAGSSSSLDYEGYCV 194
>UniRef50_Q26427 Cluster: Fibroin light chain precursor; n=2;
Obtectomera|Rep: Fibroin light chain precursor -
Galleria mellonella (Wax moth)
Length = 267
Score = 68.5 bits (160), Expect = 1e-10
Identities = 38/76 (50%), Positives = 52/76 (68%), Gaps = 2/76 (2%)
Frame = +1
Query: 28 MKPIFLVLLVATSAYAAPSVTINQYSDNEIPRDIDDGK--ASSVISRAWDYVDDTDKSIA 201
M P LVLLVATSA AAPSV I+Q + N I + +G+ +S++I RA++ VD D +I
Sbjct: 1 MLPFVLVLLVATSALAAPSVVISQDNINNIAPRVGNGRPISSALIDRAFEIVDGGDTNIY 60
Query: 202 ILNVQEILKDMASQGD 249
IL +Q+IL D+A Q D
Sbjct: 61 ILTIQQILNDLADQPD 76
Score = 58.8 bits (136), Expect = 9e-08
Identities = 31/91 (34%), Positives = 46/91 (50%)
Frame = +3
Query: 237 QPGRLCSQASAVAQTAGIIAHLSAGIPGDACAAANVINSYTDGVRSGNFXGFRQSLGPFF 416
QP L SQ+ AV Q + L+ G+PG++C AA VI++Y + VR+G+ ++ +
Sbjct: 74 QPDGL-SQSLAVTQAVAALGELATGVPGNSCEAAAVIDAYANSVRTGDNSALSIAVANYI 132
Query: 417 GHVGXXXXXXXXXXXXPGQLRYSVGPALGCA 509
+ P LRYS GPA CA
Sbjct: 133 NRLSSNIGLISQLASNPDSLRYSSGPAGNCA 163
Score = 39.9 bits (89), Expect = 0.044
Identities = 19/36 (52%), Positives = 24/36 (66%), Gaps = 4/36 (11%)
Frame = +2
Query: 500 GLC-GGGRIYDFEAAWDAILASSDS---SFLNXEYC 595
G C GGGR Y FEAAWDA+L +++ +N EYC
Sbjct: 160 GNCAGGGRSYQFEAAWDAVLNNANPYQIGLINEEYC 195
>UniRef50_Q14UU5 Cluster: Light-chain fibroin; n=1; Yponomeuta
evonymellus|Rep: Light-chain fibroin - Yponomeuta
evonymella (Bird-cherry ermine moth)
Length = 260
Score = 57.6 bits (133), Expect = 2e-07
Identities = 33/76 (43%), Positives = 48/76 (63%), Gaps = 2/76 (2%)
Frame = +1
Query: 28 MKPIFLVLLVATSAYAAPSVTINQ--YSDNEIPRDIDDGKASSVISRAWDYVDDTDKSIA 201
M P+ LVLLVA SA +APSV++NQ Y+ E PRD + S V + +D +++I
Sbjct: 1 MLPLVLVLLVAQSALSAPSVSVNQVAYNQAEGPRDNGNLINSYVTDAVFGLLDGAEQNIY 60
Query: 202 ILNVQEILKDMASQGD 249
+L Q+I+ DMA+ GD
Sbjct: 61 MLTNQQIVNDMANSGD 76
Score = 41.5 bits (93), Expect = 0.015
Identities = 18/48 (37%), Positives = 29/48 (60%), Gaps = 3/48 (6%)
Frame = +2
Query: 464 PWSTPILCRTSPGLCGGGRIYDFEAAWDAILASSDS---SFLNXEYCI 598
P + + +S G GGGR Y FE WD++LA++++ LN +YC+
Sbjct: 144 PTAAGSIVGSSGGCAGGGRSYQFEQVWDSVLANANAYTIGLLNEQYCM 191
Score = 37.9 bits (84), Expect = 0.18
Identities = 28/97 (28%), Positives = 40/97 (41%)
Frame = +3
Query: 219 DLEGHGQPGRLCSQASAVAQTAGIIAHLSAGIPGDACAAANVINSYTDGVRSGNFXGFRQ 398
D+ G P +QA A+ Q ++ + G GDACA AN+ N+Y SGN Q
Sbjct: 70 DMANSGDP---TTQALALGQAINLVGE-AVGSTGDACAYANLANAYA----SGNAAAVSQ 121
Query: 399 SLGPFFGHVGXXXXXXXXXXXXPGQLRYSVGPALGCA 509
+L + + P VG + GCA
Sbjct: 122 ALSGYVNRLNANINAVARLAVDPTAAGSIVGSSGGCA 158
>UniRef50_Q9FWL9 Cluster: Putative uncharacterized protein
OSJNBa0079L16.4; n=1; Oryza sativa|Rep: Putative
uncharacterized protein OSJNBa0079L16.4 - Oryza sativa
(Rice)
Length = 199
Score = 37.9 bits (84), Expect = 0.18
Identities = 27/71 (38%), Positives = 32/71 (45%), Gaps = 2/71 (2%)
Frame = -1
Query: 415 KKGPRDCLKPXKFPDLTPSV*ELMTLAAAQ--ASPGIPADRWAIIPAVWATADA*LHNRP 242
++ R C +P P P + E MT AAAQ A G+ D W VW A A R
Sbjct: 125 RRSARRCSRPQLLPPPPPPL-ETMTTAAAQLVAVAGLNGDCWKEASGVWPRAAAKTEQRG 183
Query: 241 GWPCPSRSLER 209
G SRS ER
Sbjct: 184 GAVAGSRSGER 194
>UniRef50_UPI0000DA2531 Cluster: PREDICTED: hypothetical protein;
n=3; Rattus norvegicus|Rep: PREDICTED: hypothetical
protein - Rattus norvegicus
Length = 393
Score = 35.1 bits (77), Expect = 1.3
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +3
Query: 222 LEGHGQPGRLCSQASAVAQTAGIIAHLSAGIPGD 323
+EGHGQP + C+QA A G++ H + P D
Sbjct: 1 MEGHGQPSQNCAQADA-EDNIGVVGHTTESSPSD 33
>UniRef50_UPI0000E45C65 Cluster: PREDICTED: hypothetical protein; n=3;
Strongylocentrotus purpuratus|Rep: PREDICTED:
hypothetical protein - Strongylocentrotus purpuratus
Length = 1073
Score = 34.7 bits (76), Expect = 1.7
Identities = 22/62 (35%), Positives = 28/62 (45%)
Frame = -3
Query: 584 HLRN*SHCWLRLHPKRLRSHRFFHLRTTQGWSDRVSELTRVDDELIDKIQVLSHVSEEGT 405
HLR L++H L HLR TQ ++E + D L DKI L + EE
Sbjct: 855 HLRKREQALLKMHQDNLDKAAAEHLRETQA---MLTEFNKAQDLLKDKISALQIMLEEAE 911
Query: 404 ER 399
ER
Sbjct: 912 ER 913
>UniRef50_A6RJB3 Cluster: Putative uncharacterized protein; n=1;
Botryotinia fuckeliana B05.10|Rep: Putative
uncharacterized protein - Botryotinia fuckeliana B05.10
Length = 333
Score = 33.1 bits (72), Expect = 5.1
Identities = 19/77 (24%), Positives = 37/77 (48%)
Frame = +1
Query: 94 NQYSDNEIPRDIDDGKASSVISRAWDYVDDTDKSIAILNVQEILKDMASQGDYAVKHQRW 273
+ Y D + ++GK A DY ++ D +L EI +DM + + + R+
Sbjct: 63 SNYDDELVEMSDEEGKGGDKEEEADDY-EEGDVVTEVLKDVEITEDMGPEERLRILYSRY 121
Query: 274 PKPPELSPIYLPVSPVM 324
P+ L+ +L ++PV+
Sbjct: 122 PEFEFLADEFLELAPVL 138
>UniRef50_UPI0000E25565 Cluster: PREDICTED: hypothetical protein;
n=1; Pan troglodytes|Rep: PREDICTED: hypothetical
protein - Pan troglodytes
Length = 422
Score = 32.7 bits (71), Expect = 6.7
Identities = 25/72 (34%), Positives = 32/72 (44%)
Frame = -1
Query: 499 RAGPTEYRS*PGLMTS*LIRFKFCPTCPKKGPRDCLKPXKFPDLTPSV*ELMTLAAAQAS 320
R G T R PG + ++R P KGPRD L P FP +P EL TL+ +
Sbjct: 150 RRGRTLARRRPGALRPSVVRRGGRPGTAAKGPRDELGP-SFPMASPPGLELKTLSNGPQA 208
Query: 319 PGIPADRWAIIP 284
P A + P
Sbjct: 209 PRRSAPLGPVAP 220
>UniRef50_A3JVU6 Cluster: Membrane protein; n=1; Rhodobacterales
bacterium HTCC2150|Rep: Membrane protein -
Rhodobacterales bacterium HTCC2150
Length = 498
Score = 32.7 bits (71), Expect = 6.7
Identities = 18/52 (34%), Positives = 29/52 (55%), Gaps = 2/52 (3%)
Frame = +3
Query: 228 GHGQP-GRLCSQASAVA-QTAGIIAHLSAGIPGDACAAANVINSYTDGVRSG 377
GHG P G + S+ S A A +I L+ G+PG+A A ++ + G++ G
Sbjct: 294 GHGSPEGLIASETSNNAVPAAAMIPLLALGVPGEALTAMMMVVFFDAGIKPG 345
>UniRef50_Q0J1I0 Cluster: Os09g0439000 protein; n=5;
Magnoliophyta|Rep: Os09g0439000 protein - Oryza sativa
subsp. japonica (Rice)
Length = 966
Score = 32.7 bits (71), Expect = 6.7
Identities = 11/31 (35%), Positives = 20/31 (64%)
Frame = +3
Query: 228 GHGQPGRLCSQASAVAQTAGIIAHLSAGIPG 320
G GR S+ + V +T G+++ +S+G+PG
Sbjct: 97 GRAPAGRAASKGAGVGETLGVVSRVSSGVPG 127
>UniRef50_Q18Q44 Cluster: TraB family protein; n=3; Firmicutes|Rep:
TraB family protein - Desulfitobacterium hafniense
(strain DCB-2)
Length = 390
Score = 32.3 bits (70), Expect = 8.9
Identities = 16/60 (26%), Positives = 32/60 (53%)
Frame = -3
Query: 491 SDRVSELTRVDDELIDKIQVLSHVSEEGTERLSEAXEVSGPDXVCVRVNDVSGCTGITGD 312
++ ++ LT E+I + +HVS++ E + E E PD VC+ +++ + + GD
Sbjct: 5 NENITRLTLAGKEII--LIGTAHVSKQSAELVKEVIEAERPDSVCIELDEQRYKSIVEGD 62
>UniRef50_A6BJ85 Cluster: Putative uncharacterized protein; n=1; Dorea
longicatena DSM 13814|Rep: Putative uncharacterized
protein - Dorea longicatena DSM 13814
Length = 1308
Score = 32.3 bits (70), Expect = 8.9
Identities = 19/68 (27%), Positives = 30/68 (44%)
Frame = -3
Query: 506 TTQGWSDRVSELTRVDDELIDKIQVLSHVSEEGTERLSEAXEVSGPDXVCVRVNDVSGCT 327
T W S L D L +K ++ TE+L++A +VND +G T
Sbjct: 1193 TADSWKALQSALAEADTALKEKKD--QDTVDKATEKLNKAISALVKKDAGQKVNDTNGTT 1250
Query: 326 GITGDTGR 303
G +G +G+
Sbjct: 1251 GTSGKSGK 1258
>UniRef50_A5L6F4 Cluster: Probable binding protein component of ABC
transporter; n=1; Vibrionales bacterium SWAT-3|Rep:
Probable binding protein component of ABC transporter -
Vibrionales bacterium SWAT-3
Length = 584
Score = 32.3 bits (70), Expect = 8.9
Identities = 17/46 (36%), Positives = 26/46 (56%)
Frame = +1
Query: 22 TKMKPIFLVLLVATSAYAAPSVTINQYSDNEIPRDIDDGKASSVIS 159
+K + + LV+LVA+S A V + +YSDN P D +A + S
Sbjct: 2 SKFRLLPLVMLVASSFAIADDVKVFKYSDNGTPTSFDTTQAGTTYS 47
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 584,871,416
Number of Sequences: 1657284
Number of extensions: 11486233
Number of successful extensions: 36245
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 34813
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 36232
length of database: 575,637,011
effective HSP length: 97
effective length of database: 414,880,463
effective search space used: 41902926763
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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