BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesS0960 (538 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g40030.1 68415.m04919 DNA-directed RNA polymerase alpha subun... 29 1.5 At1g18470.1 68414.m02304 zinc finger (C3HC4-type RING finger) fa... 29 1.5 At5g17730.1 68418.m02079 AAA-type ATPase family protein contains... 29 2.0 At5g33280.1 68418.m03944 chloride channel-like (CLC) protein, pu... 28 3.4 At2g16960.1 68415.m01954 importin beta-2 subunit family protein ... 28 4.5 At1g09420.1 68414.m01054 glucose-6-phosphate 1-dehydrogenase, pu... 28 4.5 At5g18420.3 68418.m02170 expressed protein non-consensus GC dono... 27 6.0 At5g18420.2 68418.m02169 expressed protein non-consensus GC dono... 27 6.0 At5g18420.1 68418.m02168 expressed protein non-consensus GC dono... 27 6.0 At5g41730.1 68418.m05074 protein kinase family protein contains ... 27 7.9 At3g48820.2 68416.m05332 glycosyl transferase family 29 protein ... 27 7.9 At3g48820.1 68416.m05331 glycosyl transferase family 29 protein ... 27 7.9 At3g12590.1 68416.m01568 expressed protein 27 7.9 At3g01760.1 68416.m00114 lysine and histidine specific transport... 27 7.9 >At2g40030.1 68415.m04919 DNA-directed RNA polymerase alpha subunit family protein contains InterPro accession IPR000722: RNA polymerase, alpha subunit Length = 888 Score = 29.5 bits (63), Expect = 1.5 Identities = 20/87 (22%), Positives = 45/87 (51%), Gaps = 1/87 (1%) Frame = -3 Query: 293 GFNLNPESDAVSQLDMQVMHINLLVSPSSTAVMSMEKHF-NNISEYISMVSLCLCPVNIL 117 GF+L+ E ++S+ DM V+H NL++ S V + + + + S+ + N + Sbjct: 567 GFSLSLEDLSMSRADMDVIH-NLIIREISPMVSRLRLSYRDELQLENSIHKVKEVAANFM 625 Query: 116 FTAPSLRHKLHTPSSGNISTIISESMF 36 + S+R+ + S+ I+ ++ ++ F Sbjct: 626 LKSYSIRNLIDIKSNSAITKLVQQTGF 652 >At1g18470.1 68414.m02304 zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type Length = 467 Score = 29.5 bits (63), Expect = 1.5 Identities = 12/31 (38%), Positives = 16/31 (51%) Frame = +1 Query: 181 CFSIDITAVELGDTKRLICMTCISSCETASL 273 CF DI+ V L R++C TC C T + Sbjct: 423 CFEKDISLVLLPCRHRVLCRTCADKCTTCPI 453 >At5g17730.1 68418.m02079 AAA-type ATPase family protein contains Pfam profile: ATPase family PF00004 Length = 470 Score = 29.1 bits (62), Expect = 2.0 Identities = 16/51 (31%), Positives = 25/51 (49%) Frame = -3 Query: 290 FNLNPESDAVSQLDMQVMHINLLVSPSSTAVMSMEKHFNNISEYISMVSLC 138 F LNP+ + V +L + H L+++ V S K NN + + M S C Sbjct: 144 FKLNPDRECV-ELSFEKKHTELVLNSYIPYVESKAKVINNERKILKMYSYC 193 >At5g33280.1 68418.m03944 chloride channel-like (CLC) protein, putative similar to CLC-c, At5g49890 [Arabidopsis thaliana] and chloride channel protein ClC-1 - Nicotiana tabacum, PIR:T02939 Length = 763 Score = 28.3 bits (60), Expect = 3.4 Identities = 15/50 (30%), Positives = 25/50 (50%), Gaps = 1/50 (2%) Frame = -3 Query: 197 MSMEKH-FNNISEYISMVSLCLCPVNILFTAPSLRHKLHTPSSGNISTII 51 M ++ H F+N S Y + ++ L ILF +RH L P + N ++ Sbjct: 678 MYLDLHPFSNASPYTVVETMSLAKALILFREVGIRHLLVIPKTSNRPPVV 727 >At2g16960.1 68415.m01954 importin beta-2 subunit family protein contains weak hit to Pfam PF02985: HEAT repeat (4 copies); contains weak hit to Pfam PF03130: PBS lyase HEAT-like repeat (2 copies); supported by tandem duplication of importin beta family protein (TIGR_Ath1:At2g16950) [Arabidopsis thaliana]; similar to Importin beta-2 subunit (Karyopherin beta-2 subunit) (Transportin) (M9 region interaction protein) (MIP) (Swiss-Prot:Q92973) [Homo sapiens] Length = 547 Score = 27.9 bits (59), Expect = 4.5 Identities = 19/52 (36%), Positives = 25/52 (48%) Frame = +1 Query: 82 VCSLCLNDGAVKSMLTGHKHNDTMEIYSDMLLKCFSIDITAVELGDTKRLIC 237 VCSL G K +++G + + DMLLKCF +D T LIC Sbjct: 286 VCSLEFFSGLSKGLVSGIESLVSQSNLRDMLLKCF-MDETPDVRESAFALIC 336 >At1g09420.1 68414.m01054 glucose-6-phosphate 1-dehydrogenase, putative / G6PD, putative similar to SP|Q43839 Glucose-6-phosphate 1-dehydrogenase, chloroplast precursor (EC 1.1.1.49) (G6PD) {Solanum tuberosum}; contains Pfam profiles PF02781: Glucose-6-phosphate dehydrogenase C-terminal, PF00479: Glucose-6-phosphate dehydrogenase NAD binding domain; gc exon splice site at 20574 is based on protein alignment, and is not confirmed experimentally Length = 625 Score = 27.9 bits (59), Expect = 4.5 Identities = 18/52 (34%), Positives = 29/52 (55%) Frame = -1 Query: 397 SLATTSPSASVLRSSKAPCDSTFSNLNVSSRVYKEAST*TLKATQSRNSICR 242 S + T+PS+SV A ++FSN VSSR Y + + +L +++CR Sbjct: 11 SQSATAPSSSVCSCHLA---ASFSNFPVSSRDYSFSRSGSLVLNGGGSNLCR 59 >At5g18420.3 68418.m02170 expressed protein non-consensus GC donor splice site at exon 1, unknown (C40) protein, Homo sapiens, EMBL:AF103798 Length = 439 Score = 27.5 bits (58), Expect = 6.0 Identities = 19/48 (39%), Positives = 25/48 (52%), Gaps = 6/48 (12%) Frame = +1 Query: 142 NDTMEIYSDMLLKCFSIDI-----TAVELG-DTKRLICMTCISSCETA 267 ND E +M + S+++ TAVEL D R+ CISSCE A Sbjct: 330 NDYYEALGNMDMSLHSMEVVNRLTTAVELPKDFIRMYITNCISSCENA 377 >At5g18420.2 68418.m02169 expressed protein non-consensus GC donor splice site at exon 1, unknown (C40) protein, Homo sapiens, EMBL:AF103798 Length = 442 Score = 27.5 bits (58), Expect = 6.0 Identities = 19/48 (39%), Positives = 25/48 (52%), Gaps = 6/48 (12%) Frame = +1 Query: 142 NDTMEIYSDMLLKCFSIDI-----TAVELG-DTKRLICMTCISSCETA 267 ND E +M + S+++ TAVEL D R+ CISSCE A Sbjct: 333 NDYYEALGNMDMSLHSMEVVNRLTTAVELPKDFIRMYITNCISSCENA 380 >At5g18420.1 68418.m02168 expressed protein non-consensus GC donor splice site at exon 1, unknown (C40) protein, Homo sapiens, EMBL:AF103798 Length = 441 Score = 27.5 bits (58), Expect = 6.0 Identities = 19/48 (39%), Positives = 25/48 (52%), Gaps = 6/48 (12%) Frame = +1 Query: 142 NDTMEIYSDMLLKCFSIDI-----TAVELG-DTKRLICMTCISSCETA 267 ND E +M + S+++ TAVEL D R+ CISSCE A Sbjct: 333 NDYYEALGNMDMSLHSMEVVNRLTTAVELPKDFIRMYITNCISSCENA 380 >At5g41730.1 68418.m05074 protein kinase family protein contains Pfam PF00069: Protein kinase domain Length = 711 Score = 27.1 bits (57), Expect = 7.9 Identities = 12/37 (32%), Positives = 20/37 (54%) Frame = -1 Query: 400 ISLATTSPSASVLRSSKAPCDSTFSNLNVSSRVYKEA 290 +S+ P+A + R +K+ C T S + + VY EA Sbjct: 587 VSVVEDPPNAMITRDTKSLCLDTISEYSDTRSVYSEA 623 >At3g48820.2 68416.m05332 glycosyl transferase family 29 protein / sialyltransferase family protein contains Pfam profile: PF00777 sialyltransferase (Glycosyltransferase family 29) Length = 431 Score = 27.1 bits (57), Expect = 7.9 Identities = 11/35 (31%), Positives = 18/35 (51%) Frame = +1 Query: 247 ISSCETASLSGFKLKPPYTLWRIHLS*RM*NHTAL 351 +S+C++ + GF + P Y W + S HT L Sbjct: 283 LSTCDSVDMYGFTVDPGYKEWTRYFSESRQGHTPL 317 >At3g48820.1 68416.m05331 glycosyl transferase family 29 protein / sialyltransferase family protein contains Pfam profile: PF00777 sialyltransferase (Glycosyltransferase family 29) Length = 440 Score = 27.1 bits (57), Expect = 7.9 Identities = 11/35 (31%), Positives = 18/35 (51%) Frame = +1 Query: 247 ISSCETASLSGFKLKPPYTLWRIHLS*RM*NHTAL 351 +S+C++ + GF + P Y W + S HT L Sbjct: 292 LSTCDSVDMYGFTVDPGYKEWTRYFSESRQGHTPL 326 >At3g12590.1 68416.m01568 expressed protein Length = 1162 Score = 27.1 bits (57), Expect = 7.9 Identities = 12/30 (40%), Positives = 19/30 (63%) Frame = +1 Query: 202 AVELGDTKRLICMTCISSCETASLSGFKLK 291 +V+ GDTK +S+CETAS++ L+ Sbjct: 983 SVDEGDTKPRYNNVSVSTCETASMAAMLLR 1012 >At3g01760.1 68416.m00114 lysine and histidine specific transporter, putative similar to lysine and histidine specific transporter [Arabidopsis thaliana] GI:2576361; contains Pfam profile PF01490: Transmembrane amino acid transporter protein Length = 479 Score = 27.1 bits (57), Expect = 7.9 Identities = 22/63 (34%), Positives = 37/63 (58%), Gaps = 5/63 (7%) Frame = -3 Query: 317 CILQSV*GGFNLNPESD-AVSQLD----MQVMHINLLVSPSSTAVMSMEKHFNNISEYIS 153 CI+ V GG +L D A+ D +++ H +L+ SS V+S+ K+FN+IS +S Sbjct: 153 CIVYMVTGGKSLKNVHDLALGDGDKCTKLRIQHF-ILIFASSQFVLSLLKNFNSISG-VS 210 Query: 152 MVS 144 +V+ Sbjct: 211 LVA 213 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,331,975 Number of Sequences: 28952 Number of extensions: 190847 Number of successful extensions: 448 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 444 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 448 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 993966856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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