Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesS0941
(548 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g19900.1 68415.m02326 malate oxidoreductase, putative similar... 87 6e-18
At5g25880.1 68418.m03071 malate oxidoreductase, putative similar... 84 5e-17
At5g11670.1 68418.m01364 malate oxidoreductase, putative similar... 81 4e-16
At1g79750.1 68414.m09304 malate oxidoreductase, putative similar... 74 7e-14
At4g00570.1 68417.m00080 malate oxidoreductase, putative similar... 72 2e-13
At2g13560.1 68415.m01495 malate oxidoreductase, putative similar... 71 7e-13
At3g29796.1 68416.m03790 hypothetical protein 28 4.7
At3g44770.1 68416.m04820 hypothetical protein contains Pfam prof... 27 8.3
At2g05290.1 68415.m00557 expressed protein similar to zinc finge... 27 8.3
At1g08135.1 68414.m00894 cation/hydrogen exchanger (CHX6b) Note:... 27 8.3
>At2g19900.1 68415.m02326 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP:P51615) {Vitis vinifera}
Length = 581
Score = 87.4 bits (207), Expect = 6e-18
Identities = 37/59 (62%), Positives = 50/59 (84%)
Frame = +3
Query: 345 TELQDRNEKLFFSLLDCDIEKFMPIVYTPTVGLACQKFGLVYRRPRGLYITIXDKGTYL 521
TELQ+RNE+LF+ LL ++E+ +PIVYTPTVG ACQKFG ++RRP+GL+I++ DKG L
Sbjct: 103 TELQERNERLFYKLLIDNVEELLPIVYTPTVGEACQKFGSIFRRPQGLFISLKDKGKIL 161
Score = 29.1 bits (62), Expect = 2.0
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = +2
Query: 164 GIDHIKDPRLNKGLAFTLEE 223
G ++DPR NKGLAFT +E
Sbjct: 42 GYSLLRDPRYNKGLAFTEKE 61
>At5g25880.1 68418.m03071 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP:P12628) {Phaseolus vulgaris}
Length = 588
Score = 84.2 bits (199), Expect = 5e-17
Identities = 34/58 (58%), Positives = 49/58 (84%)
Frame = +3
Query: 348 ELQDRNEKLFFSLLDCDIEKFMPIVYTPTVGLACQKFGLVYRRPRGLYITIXDKGTYL 521
+LQ+RNE+LF+ LL ++E+ +P+VYTPTVG ACQK+G +YRRP+GLYI++ +KG L
Sbjct: 111 DLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLYISLKEKGKIL 168
Score = 28.7 bits (61), Expect = 2.7
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = +2
Query: 164 GIDHIKDPRLNKGLAFTLEEPKL*VFTDFWPQI 262
G ++DPR NKGLAFT +E T P +
Sbjct: 49 GYTLMRDPRYNKGLAFTDKERDAHYITGLLPPV 81
>At5g11670.1 68418.m01364 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP|P12628) {Phaseolus vulgaris}
Length = 588
Score = 81.4 bits (192), Expect = 4e-16
Identities = 32/58 (55%), Positives = 49/58 (84%)
Frame = +3
Query: 348 ELQDRNEKLFFSLLDCDIEKFMPIVYTPTVGLACQKFGLVYRRPRGLYITIXDKGTYL 521
+LQ+RNE+LF+ LL ++E+ +P+VYTPTVG ACQK+G ++R+P+GLYI++ +KG L
Sbjct: 111 DLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRKPQGLYISLNEKGKIL 168
Score = 28.7 bits (61), Expect = 2.7
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = +2
Query: 164 GIDHIKDPRLNKGLAFTLEEPKL*VFTDFWPQI 262
G ++DPR NKGLAFT +E T P +
Sbjct: 49 GYTLMRDPRYNKGLAFTDKERDAHYLTGLLPPV 81
>At1g79750.1 68414.m09304 malate oxidoreductase, putative similar to
malate oxidoreductase (NADP-dependent malic enzyme)
GB:P34105 (Populus balsamifera subsp. trichocarpa)
Length = 646
Score = 73.7 bits (173), Expect = 7e-14
Identities = 28/55 (50%), Positives = 45/55 (81%)
Frame = +3
Query: 348 ELQDRNEKLFFSLLDCDIEKFMPIVYTPTVGLACQKFGLVYRRPRGLYITIXDKG 512
+LQ+ NE+LF+ LL +E+ +P++YTPTVG ACQK+G ++ RP+GL+I++ +KG
Sbjct: 169 DLQETNERLFYKLLIDHVEELLPVIYTPTVGEACQKYGSIFLRPQGLFISLKEKG 223
>At4g00570.1 68417.m00080 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 59 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37225) {Solanum
tuberosum}
Length = 607
Score = 72.1 bits (169), Expect = 2e-13
Identities = 30/54 (55%), Positives = 40/54 (74%)
Frame = +3
Query: 351 LQDRNEKLFFSLLDCDIEKFMPIVYTPTVGLACQKFGLVYRRPRGLYITIXDKG 512
L DRNE L++ +L +I+ F PI+YTPTVGL CQ + +YRRPRG+Y + DKG
Sbjct: 112 LHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLYRRPRGMYFSAKDKG 165
>At2g13560.1 68415.m01495 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 62 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37221) {Solanum
tuberosum}
Length = 623
Score = 70.5 bits (165), Expect = 7e-13
Identities = 28/54 (51%), Positives = 41/54 (75%)
Frame = +3
Query: 351 LQDRNEKLFFSLLDCDIEKFMPIVYTPTVGLACQKFGLVYRRPRGLYITIXDKG 512
L DRNE +++ +L +IE++ PIVYTPTVGL CQ + ++RRPRG+Y + D+G
Sbjct: 119 LHDRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRG 172
Score = 28.3 bits (60), Expect = 3.6
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +2
Query: 161 RGIDHIKDPRLNKGLAFTLEE 223
+G+D + DP NKG AFT+ E
Sbjct: 45 QGLDILHDPWFNKGTAFTMTE 65
>At3g29796.1 68416.m03790 hypothetical protein
Length = 463
Score = 27.9 bits (59), Expect = 4.7
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +2
Query: 23 FKSFLTFIFERTKFKRNIINGLQPKYLDQ 109
F +FL I T+F RN + L+ KYL Q
Sbjct: 363 FATFLRGIMTSTQFPRNCLANLRGKYLSQ 391
>At3g44770.1 68416.m04820 hypothetical protein contains Pfam profile
PF04776: Protein of unknown function (DUF626)
Length = 505
Score = 27.1 bits (57), Expect = 8.3
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = -3
Query: 132 VISCIFLLWSKYLGCKPFIIXRLNFVLSKINVK 34
+++ + LW +LG KP I NF LSK+ +
Sbjct: 377 LVATMISLWMMFLGEKPEFIDFDNFELSKVTFR 409
>At2g05290.1 68415.m00557 expressed protein similar to zinc finger
protein [Arabidopsis thaliana] GI:976277
Length = 383
Score = 27.1 bits (57), Expect = 8.3
Identities = 14/41 (34%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = +2
Query: 74 IINGLQPKYLDQRRNMHEITGNIICPNMLRGIDHIKD-PRL 193
+IN QP + RN++ I G + P+ R I D PR+
Sbjct: 22 LINSDQPNFFSTERNLNSIMGRFLNPDKQRMSKWILDMPRI 62
>At1g08135.1 68414.m00894 cation/hydrogen exchanger (CHX6b) Note:
CHX6a and CHX6b were originally 1 gene but were split
pased on alignments with other family members; may be a
pseudogene and requires futher investigation; monovalent
cation:proton antiporter family 2 (CPA2) member,
PMID:11500563
Length = 824
Score = 27.1 bits (57), Expect = 8.3
Identities = 12/35 (34%), Positives = 20/35 (57%)
Frame = -3
Query: 156 MFGQIMLPVISCIFLLWSKYLGCKPFIIXRLNFVL 52
MFG + +P + IF+ +++Y+ P I FVL
Sbjct: 132 MFGSMQIPCLKHIFIHYNQYMTKVPDTIGAFAFVL 166
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,592,048
Number of Sequences: 28952
Number of extensions: 203728
Number of successful extensions: 443
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 427
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 443
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1033331880
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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