Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesS0658
(538 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 42 3e-04
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 40 8e-04
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 39 0.002
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 34 0.069
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 33 0.091
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 31 0.49
At1g78910.1 68414.m09199 pseudouridine synthase family protein l... 29 1.5
At4g13700.1 68417.m02128 serine/threonine protein phosphatase fa... 29 2.0
At1g08370.1 68414.m00926 hydroxyproline-rich glycoprotein family... 29 2.6
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 28 3.4
At2g27980.1 68415.m03391 expressed protein 28 3.4
At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containi... 27 6.0
At5g24830.1 68418.m02934 pentatricopeptide (PPR) repeat-containi... 27 7.9
>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
(HSP22.0-ER) identical to endomembrane-localized small
heat shock protein GI:511795 from [Arabidopsis thaliana]
Length = 195
Score = 41.5 bits (93), Expect = 3e-04
Identities = 30/99 (30%), Positives = 54/99 (54%), Gaps = 8/99 (8%)
Frame = +3
Query: 264 KSDKDKFQVNLDVQHFAPEEISVKTXD-GYIVVEG---KHEEKK-DQHGYISR---QFTR 419
K + ++ LD+ +E+ ++ + G + V G + EEKK DQ + R +F R
Sbjct: 76 KETAEGHEIMLDIPGLKKDEVKIEVEENGVLRVSGERKREEEKKGDQWHRVERSYGKFWR 135
Query: 420 RYALPEGCTAESVESRLSSDGVLSVIRTQEVPPAVEGER 536
++ LP+ ESV+++L +GVL++ T+ P V+G R
Sbjct: 136 QFKLPDNVDMESVKAKL-ENGVLTINLTKLSPEKVKGPR 173
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 40.3 bits (90), Expect = 8e-04
Identities = 31/87 (35%), Positives = 46/87 (52%), Gaps = 10/87 (11%)
Frame = +3
Query: 297 DVQHFAPEEISVKTXDGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 452
DV EE+ V+ DG I+ + G+ +EEK D + R +F RR+ LPE E
Sbjct: 65 DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124
Query: 453 SVESRLSSDGVLSVI--RTQEVPPAVE 527
V++ + +GVLSV + QE P V+
Sbjct: 125 EVKASM-ENGVLSVTVPKVQESKPEVK 150
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 39.1 bits (87), Expect = 0.002
Identities = 26/74 (35%), Positives = 42/74 (56%), Gaps = 8/74 (10%)
Frame = +3
Query: 297 DVQHFAPEEISVKTXDGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 452
D+ EE+ V+ DG I+ + G+ +EEK D+ + R +FTRR+ LPE E
Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125
Query: 453 SVESRLSSDGVLSV 494
+++ + +GVLSV
Sbjct: 126 EIKASM-ENGVLSV 138
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 33.9 bits (74), Expect = 0.069
Identities = 26/87 (29%), Positives = 46/87 (52%), Gaps = 10/87 (11%)
Frame = +3
Query: 297 DVQHFAPEEISVKTXDGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 452
D+ EE+ V+ D ++ + G+ +EEK D+ + R +F RR+ LPE E
Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127
Query: 453 SVESRLSSDGVLSVI--RTQEVPPAVE 527
V++ + +GVL+V+ + E P V+
Sbjct: 128 EVKATM-ENGVLTVVVPKAPEKKPQVK 153
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 33.5 bits (73), Expect = 0.091
Identities = 24/74 (32%), Positives = 40/74 (54%), Gaps = 8/74 (10%)
Frame = +3
Query: 297 DVQHFAPEEISVKTX-DGYIVVEG-KHEEKKDQHGYISR------QFTRRYALPEGCTAE 452
D+ EE+ V+ D + + G +H EK+D++ R QFTRR+ LPE +
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 453 SVESRLSSDGVLSV 494
V++ + +GVL+V
Sbjct: 122 QVKAAM-ENGVLTV 134
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 31.1 bits (67), Expect = 0.49
Identities = 22/74 (29%), Positives = 40/74 (54%), Gaps = 8/74 (10%)
Frame = +3
Query: 297 DVQHFAPEEISVKTXDGYIV-VEGKH----EEKKDQHGYISR---QFTRRYALPEGCTAE 452
D+ EE+ V+ D ++ + G+ EEK+D + R QF+R++ LPE +
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 453 SVESRLSSDGVLSV 494
V++ + +GVL+V
Sbjct: 124 QVKASM-ENGVLTV 136
>At1g78910.1 68414.m09199 pseudouridine synthase family protein low
similarity to SP|P23851 Ribosomal large subunit
pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
synthase) (Uracil hydrolyase) {Escherichia coli};
contains Pfam profile PF00849: RNA pseudouridylate
synthase
Length = 478
Score = 29.5 bits (63), Expect = 1.5
Identities = 29/93 (31%), Positives = 38/93 (40%), Gaps = 2/93 (2%)
Frame = -2
Query: 462 TPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPR--CSRPPF*QRFLPGRNAGRPGL 289
T I+ YS A I N A L P+ PR S PPF LPG+NAG G
Sbjct: 14 TVLIRRYSRVAPPPSSVIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKNAGADGD 73
Query: 288 LGTYPYHF*CWTKVSSRGSKVHPRAVVFSAHKG 190
G H + V +++ +A+ KG
Sbjct: 74 DGDSGGHVTAISWVKYYFEEIYDKAIQTHFTKG 106
>At4g13700.1 68417.m02128 serine/threonine protein phosphatase
family protein contains Pfam domain PF00149: Ser/Thr
protein phosphatase
Length = 474
Score = 29.1 bits (62), Expect = 2.0
Identities = 20/79 (25%), Positives = 36/79 (45%)
Frame = +3
Query: 198 EQRILPPVGAPCCRGSRPWSSIKSDKDKFQVNLDVQHFAPEEISVKTXDGYIVVEGKHEE 377
+ R + G PC S P + I+ + +Q D E ++ K +V+EG HE
Sbjct: 233 QYRTIGGKGVPCFSCSFPDAPIR---ETYQPRWDAWGRFMEPLTSKVPT--MVIEGNHEI 287
Query: 378 KKDQHGYISRQFTRRYALP 434
+ G + ++ R+A+P
Sbjct: 288 EPQASGITFKSYSERFAVP 306
>At1g08370.1 68414.m00926 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965; contains some similarity to
transcription factor [Danio rerio]
gi|15617376|emb|CAC69871
Length = 367
Score = 28.7 bits (61), Expect = 2.6
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = -2
Query: 513 APPGCELQTTHHLKTAGTPQIQPYSPP 433
APP +H ++ GTP +QP+ PP
Sbjct: 294 APPLNPNNASHQQRSYGTPVLQPFPPP 320
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 28.3 bits (60), Expect = 3.4
Identities = 17/54 (31%), Positives = 23/54 (42%)
Frame = +3
Query: 318 EEISVKTXDGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSD 479
+EI D Y EG EE+ ++ R + L E C E+VES D
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDD 101
>At2g27980.1 68415.m03391 expressed protein
Length = 1037
Score = 28.3 bits (60), Expect = 3.4
Identities = 16/46 (34%), Positives = 25/46 (54%)
Frame = +3
Query: 228 PCCRGSRPWSSIKSDKDKFQVNLDVQHFAPEEISVKTXDGYIVVEG 365
PC R +R S +K + K +VN D H PE+ ++ + D + V G
Sbjct: 205 PCKRLTR--SMLKVEGIKSEVNADDDHVNPEKDAIGSEDNCVDVSG 248
>At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587, post-transcriptional control of
chloroplast gene expression CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 991
Score = 27.5 bits (58), Expect = 6.0
Identities = 15/48 (31%), Positives = 20/48 (41%)
Frame = -2
Query: 483 HHLKTAGTPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPRCSRP 340
HHL + ++ P S P S + H SS PRCS+P
Sbjct: 26 HHLALSSKARVFPLSLPCNFSSRVSFKLQLHCAASSSSSVSPPRCSKP 73
>At5g24830.1 68418.m02934 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 593
Score = 27.1 bits (57), Expect = 7.9
Identities = 9/27 (33%), Positives = 14/27 (51%)
Frame = -2
Query: 258 WTKVSSRGSKVHPRAVVFSAHKGAGCS 178
W K R +K+HP ++ GA C+
Sbjct: 461 WVKNEMRSTKIHPDTTTYNLLLGAACT 487
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,364,444
Number of Sequences: 28952
Number of extensions: 272945
Number of successful extensions: 702
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 681
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 702
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 993966856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -