Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesS0654
(598 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 42 2e-04
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 41 5e-04
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 37 0.009
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 37 0.012
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 35 0.036
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 34 0.063
At2g27980.1 68415.m03391 expressed protein 30 1.3
At1g78910.1 68414.m09199 pseudouridine synthase family protein l... 29 2.4
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 29 3.1
At3g22410.1 68416.m02827 expressed protein 27 7.2
At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containi... 27 7.2
At1g34180.1 68414.m04239 no apical meristem (NAM) family protein... 27 7.2
At4g02400.1 68417.m00324 U3 ribonucleoprotein (Utp) family prote... 27 9.5
At2g01960.1 68415.m00131 expressed protein 27 9.5
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 42.3 bits (95), Expect = 2e-04
Identities = 27/78 (34%), Positives = 44/78 (56%), Gaps = 8/78 (10%)
Frame = +3
Query: 297 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 452
D+ EE+ V+ DG I+ + G+ +EEK D+ + R +FTRR+ LPE E
Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125
Query: 453 SVESRLSSDGVLSVIAPR 506
+++ + +GVLSV P+
Sbjct: 126 EIKASM-ENGVLSVTVPK 142
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 41.1 bits (92), Expect = 5e-04
Identities = 28/78 (35%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
Frame = +3
Query: 297 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 452
DV EE+ V+ DG I+ + G+ +EEK D + R +F RR+ LPE E
Sbjct: 65 DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124
Query: 453 SVESRLSSDGVLSVIAPR 506
V++ + +GVLSV P+
Sbjct: 125 EVKASM-ENGVLSVTVPK 141
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 37.1 bits (82), Expect = 0.009
Identities = 24/78 (30%), Positives = 43/78 (55%), Gaps = 8/78 (10%)
Frame = +3
Query: 297 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 452
D+ EE+ V+ D ++ + G+ +EEK D+ + R +F RR+ LPE E
Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127
Query: 453 SVESRLSSDGVLSVIAPR 506
V++ + +GVL+V+ P+
Sbjct: 128 EVKATM-ENGVLTVVVPK 144
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 36.7 bits (81), Expect = 0.012
Identities = 25/78 (32%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
Frame = +3
Query: 297 DVQHFAPEEISVKTA-DGYIVVEG-KHEEKKDQHGYISR------QFTRRYALPEGCTAE 452
D+ EE+ V+ D + + G +H EK+D++ R QFTRR+ LPE +
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 453 SVESRLSSDGVLSVIAPR 506
V++ + +GVL+V P+
Sbjct: 122 QVKAAM-ENGVLTVTVPK 138
>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
(HSP22.0-ER) identical to endomembrane-localized small
heat shock protein GI:511795 from [Arabidopsis thaliana]
Length = 195
Score = 35.1 bits (77), Expect = 0.036
Identities = 25/85 (29%), Positives = 47/85 (55%), Gaps = 8/85 (9%)
Frame = +3
Query: 264 KSDKDKFQVNLDVQHFAPEEISVKTAD-GYIVVEG---KHEEKK-DQHGYISR---QFTR 419
K + ++ LD+ +E+ ++ + G + V G + EEKK DQ + R +F R
Sbjct: 76 KETAEGHEIMLDIPGLKKDEVKIEVEENGVLRVSGERKREEEKKGDQWHRVERSYGKFWR 135
Query: 420 RYALPEGCTAESVESRLSSDGVLSV 494
++ LP+ ESV+++L +GVL++
Sbjct: 136 QFKLPDNVDMESVKAKL-ENGVLTI 159
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 34.3 bits (75), Expect = 0.063
Identities = 23/78 (29%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
Frame = +3
Query: 297 DVQHFAPEEISVKTADGYIV-VEGKH----EEKKDQHGYISR---QFTRRYALPEGCTAE 452
D+ EE+ V+ D ++ + G+ EEK+D + R QF+R++ LPE +
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 453 SVESRLSSDGVLSVIAPR 506
V++ + +GVL+V P+
Sbjct: 124 QVKASM-ENGVLTVTVPK 140
>At2g27980.1 68415.m03391 expressed protein
Length = 1037
Score = 29.9 bits (64), Expect = 1.3
Identities = 17/48 (35%), Positives = 26/48 (54%)
Frame = +3
Query: 222 ASPCCRGSRPWSSIKSDKDKFQVNLDVQHFAPEEISVKTADGYIVVEG 365
A PC R +R S +K + K +VN D H PE+ ++ + D + V G
Sbjct: 203 AKPCKRLTR--SMLKVEGIKSEVNADDDHVNPEKDAIGSEDNCVDVSG 248
>At1g78910.1 68414.m09199 pseudouridine synthase family protein low
similarity to SP|P23851 Ribosomal large subunit
pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
synthase) (Uracil hydrolyase) {Escherichia coli};
contains Pfam profile PF00849: RNA pseudouridylate
synthase
Length = 478
Score = 29.1 bits (62), Expect = 2.4
Identities = 23/59 (38%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = -2
Query: 462 TPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPR--CSRPPF*QRFLPGRNAGRPG 292
T I+ YS A I N A L P+ PR S PPF LPG+NAG G
Sbjct: 14 TVLIRRYSRVAPPPSSVIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKNAGADG 72
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 28.7 bits (61), Expect = 3.1
Identities = 17/54 (31%), Positives = 23/54 (42%)
Frame = +3
Query: 318 EEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSD 479
+EI D Y EG EE+ ++ R + L E C E+VES D
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDD 101
>At3g22410.1 68416.m02827 expressed protein
Length = 400
Score = 27.5 bits (58), Expect = 7.2
Identities = 17/68 (25%), Positives = 30/68 (44%)
Frame = +3
Query: 180 CSRPPCEQRILPPVASPCCRGSRPWSSIKSDKDKFQVNLDVQHFAPEEISVKTADGYIVV 359
C+R C +R L + ++ SS S ++ N D++ EE S + +DG +
Sbjct: 29 CNRE-CVERFLKVKGDNVKKAAKQLSSCLS----WRQNFDIERLGAEEFSTELSDGVAYI 83
Query: 360 EGKHEEKK 383
G E +
Sbjct: 84 SGHDRESR 91
>At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587, post-transcriptional control of
chloroplast gene expression CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 991
Score = 27.5 bits (58), Expect = 7.2
Identities = 15/48 (31%), Positives = 20/48 (41%)
Frame = -2
Query: 483 HHLKTAGTPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPRCSRP 340
HHL + ++ P S P S + H SS PRCS+P
Sbjct: 26 HHLALSSKARVFPLSLPCNFSSRVSFKLQLHCAASSSSSVSPPRCSKP 73
>At1g34180.1 68414.m04239 no apical meristem (NAM) family protein
contains Pfam PF02365: No apical meristem (NAM) domain;
similar to NAM-like protein GI:8809651 from (Arabidopsis
thaliana)
Length = 564
Score = 27.5 bits (58), Expect = 7.2
Identities = 16/50 (32%), Positives = 24/50 (48%)
Frame = +3
Query: 357 VEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSDGVLSVIAPR 506
+E E D G RQF +P+G +AE ++S L ++ I PR
Sbjct: 229 IEKLLNEIPDAPGVNQRQFDEFVGVPQGNSAEVIQSTLLNNSSGEYIDPR 278
>At4g02400.1 68417.m00324 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 858
Score = 27.1 bits (57), Expect = 9.5
Identities = 17/50 (34%), Positives = 22/50 (44%)
Frame = +3
Query: 333 KTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSDG 482
+ D Y EG EE+ ++ R Y LPE E+VES DG
Sbjct: 52 ENVDLYEYEEGVPEEESKKNNRYDRVDNYDYELPEDFEDENVESDDDEDG 101
>At2g01960.1 68415.m00131 expressed protein
Length = 260
Score = 27.1 bits (57), Expect = 9.5
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +3
Query: 165 RFSQCCSRPPCEQRILPPVASPCC 236
RF + PP ++R+LP V + CC
Sbjct: 147 RFVNSYALPPYDRRLLPSVKTGCC 170
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,801,871
Number of Sequences: 28952
Number of extensions: 305582
Number of successful extensions: 821
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 793
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 821
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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