Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesS0629
(648 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g10050.1 68416.m01205 threonine ammonia-lyase / threonine deh... 77 1e-14
At4g11640.1 68417.m01861 serine racemase, putative similar to se... 60 1e-09
At4g29840.2 68417.m04247 threonine synthase, chloroplast identic... 44 1e-04
At4g29840.1 68417.m04248 threonine synthase, chloroplast identic... 44 1e-04
At1g72810.1 68414.m08417 threonine synthase, putative strong sim... 44 1e-04
At1g55880.2 68414.m06409 pyridoxal-5'-phosphate-dependent enzyme... 36 0.018
At1g55880.1 68414.m06408 pyridoxal-5'-phosphate-dependent enzyme... 36 0.018
At5g54810.1 68418.m06827 tryptophan synthase, beta subunit 1 (TS... 36 0.023
At4g27070.1 68417.m03892 tryptophan synthase, beta subunit 2 (TS... 36 0.023
At1g52855.1 68414.m05976 expressed protein 33 0.12
At5g28237.2 68418.m03423 tryptophan synthase, beta subunit, puta... 32 0.29
At5g28237.1 68418.m03422 tryptophan synthase, beta subunit, puta... 32 0.29
At4g08450.1 68417.m01393 disease resistance protein (TIR-NBS-LRR... 30 1.5
At5g64600.1 68418.m08118 expressed protein similar to axi 1 [Nic... 28 4.7
At3g46420.1 68416.m05032 leucine-rich repeat family protein / pr... 28 6.1
At2g03780.1 68415.m00338 translin family protein similar to SP|Q... 28 6.1
At1g09040.1 68414.m01008 expressed protein 28 6.1
At2g47070.1 68415.m05881 squamosa promoter-binding protein-like ... 27 8.1
>At3g10050.1 68416.m01205 threonine ammonia-lyase / threonine
dehydratase / threonine deaminase (OMR1) identical to
SP|Q9ZSS6 Threonine dehydratase biosynthetic,
chloroplast precursor (EC 4.3.1.19, formerly EC
4.2.1.16) (Threonine deaminase) (TD) {Arabidopsis
thaliana}
Length = 592
Score = 77.0 bits (181), Expect = 1e-14
Identities = 43/122 (35%), Positives = 64/122 (52%)
Frame = +2
Query: 257 LGMDIYFKQEFLQYTGSFKERGVRNALISLSDEQKKNGVIAASTGNHGAALSYHSTQLGI 436
LG+ +Y K+E LQ SFK RG N ++ L +Q GVI +S GNH ++ +++LG
Sbjct: 123 LGVRMYLKREDLQPVFSFKLRGAYNMMVKLPADQLAKGVICSSAGNHAQGVALSASKLGC 182
Query: 437 PCIVVVPIHTALNKVNKCEQLGAKFSRHGIDMSAAKLHAMSLGKEKEMILH*TAMDHPDV 616
++V+P+ T K E LGA G A+ HA + E+E + DHPDV
Sbjct: 183 TAVIVMPVTTPEIKWQAVENLGATVVLFGDSYDQAQAHA-KIRAEEEGLTFIPPFDHPDV 241
Query: 617 FS 622
+
Sbjct: 242 IA 243
>At4g11640.1 68417.m01861 serine racemase, putative similar to
serine racemase [Mus musculus] GI:6448865; contains Pfam
profile PF00291: Pyridoxal-phosphate dependent enzyme
Length = 346
Score = 60.1 bits (139), Expect = 1e-09
Identities = 37/112 (33%), Positives = 57/112 (50%)
Frame = +2
Query: 251 RGLGMDIYFKQEFLQYTGSFKERGVRNALISLSDEQKKNGVIAASTGNHGAALSYHSTQL 430
+G+ ++ + +G+FK RG NA++SL EQ GV+ S+GNH AALS +
Sbjct: 54 KGIECRSFYFSVYCGKSGAFKFRGACNAVLSLDAEQAAKGVVTHSSGNHAAALSLAAKIQ 113
Query: 431 GIPCIVVVPIHTALNKVNKCEQLGAKFSRHGIDMSAAKLHAMSLGKEKEMIL 586
GIP +VVP KV+ + G K MS+ + A + +E +L
Sbjct: 114 GIPAYIVVPKGAPKCKVDNVIRYGGKVIWSEATMSSREEIASKVLQETGSVL 165
>At4g29840.2 68417.m04247 threonine synthase, chloroplast identical
to SP|Q9S7B5 Threonine synthase, chloroplast precursor
(EC 4.2.3.1) (TS) {Arabidopsis thaliana}
Length = 535
Score = 43.6 bits (98), Expect = 1e-04
Identities = 30/73 (41%), Positives = 42/73 (57%), Gaps = 6/73 (8%)
Frame = +2
Query: 257 LGM-DIYFKQEFLQYTGSFKERGVRNALISLSDEQKKN-----GVIAASTGNHGAALSYH 418
LGM D++ K + +TGSFK+ G+ L+S + +K GV ASTG+ AALS +
Sbjct: 184 LGMNDLWVKHCGISHTGSFKDLGM-TVLVSQVNRLRKMKRPVVGVGCASTGDTSAALSAY 242
Query: 419 STQLGIPCIVVVP 457
GIP IV +P
Sbjct: 243 CASAGIPSIVFLP 255
>At4g29840.1 68417.m04248 threonine synthase, chloroplast identical
to SP|Q9S7B5 Threonine synthase, chloroplast precursor
(EC 4.2.3.1) (TS) {Arabidopsis thaliana}
Length = 526
Score = 43.6 bits (98), Expect = 1e-04
Identities = 30/73 (41%), Positives = 42/73 (57%), Gaps = 6/73 (8%)
Frame = +2
Query: 257 LGM-DIYFKQEFLQYTGSFKERGVRNALISLSDEQKKN-----GVIAASTGNHGAALSYH 418
LGM D++ K + +TGSFK+ G+ L+S + +K GV ASTG+ AALS +
Sbjct: 184 LGMNDLWVKHCGISHTGSFKDLGM-TVLVSQVNRLRKMKRPVVGVGCASTGDTSAALSAY 242
Query: 419 STQLGIPCIVVVP 457
GIP IV +P
Sbjct: 243 CASAGIPSIVFLP 255
>At1g72810.1 68414.m08417 threonine synthase, putative strong
similarity to SP|Q9S7B5 Threonine synthase, chloroplast
precursor (EC 4.2.3.1) (TS) {Arabidopsis thaliana}
Length = 516
Score = 43.6 bits (98), Expect = 1e-04
Identities = 26/68 (38%), Positives = 39/68 (57%), Gaps = 4/68 (5%)
Frame = +2
Query: 266 DIYFKQEFLQYTGSFKERGVRNALISLSDEQKKN----GVIAASTGNHGAALSYHSTQLG 433
D++ K + +TGSFK+ G+ + ++ +K N GV ASTG+ AALS + G
Sbjct: 179 DLWVKHCGISHTGSFKDLGMSVLVSQVNRLRKMNKPVIGVGCASTGDTSAALSAYCASAG 238
Query: 434 IPCIVVVP 457
IP IV +P
Sbjct: 239 IPSIVFLP 246
>At1g55880.2 68414.m06409 pyridoxal-5'-phosphate-dependent enzyme,
beta family protein similar to SP|P50867 Cysteine
synthase (EC 4.2.99.8) (O-acetylserine sulfhydrylase)
(O-acetylserine (Thiol)-lyase) [Aspergillus nidulans]
{Emericella nidulans}; contains Pfam profile PF00291:
Pyridoxal-phosphate dependent enzyme
Length = 348
Score = 36.3 bits (80), Expect = 0.018
Identities = 30/90 (33%), Positives = 42/90 (46%), Gaps = 4/90 (4%)
Frame = +2
Query: 260 GMDIYFKQEFLQYTGSFKERGVRNALISLSDEQKK---NGVIA-ASTGNHGAALSYHSTQ 427
G +I K EFL GS K+R V +I + E K G++ S G+ +L+ +
Sbjct: 64 GCEILGKCEFLNPGGSVKDR-VAVKIIQEALESGKLFPGGIVTEGSAGSTAISLATVAPA 122
Query: 428 LGIPCIVVVPIHTALNKVNKCEQLGAKFSR 517
G C VV+P A+ K E LGA R
Sbjct: 123 YGCKCHVVIPDDAAIEKSQIIEALGASVER 152
>At1g55880.1 68414.m06408 pyridoxal-5'-phosphate-dependent enzyme,
beta family protein similar to SP|P50867 Cysteine
synthase (EC 4.2.99.8) (O-acetylserine sulfhydrylase)
(O-acetylserine (Thiol)-lyase) [Aspergillus nidulans]
{Emericella nidulans}; contains Pfam profile PF00291:
Pyridoxal-phosphate dependent enzyme
Length = 421
Score = 36.3 bits (80), Expect = 0.018
Identities = 30/90 (33%), Positives = 42/90 (46%), Gaps = 4/90 (4%)
Frame = +2
Query: 260 GMDIYFKQEFLQYTGSFKERGVRNALISLSDEQKK---NGVIA-ASTGNHGAALSYHSTQ 427
G +I K EFL GS K+R V +I + E K G++ S G+ +L+ +
Sbjct: 64 GCEILGKCEFLNPGGSVKDR-VAVKIIQEALESGKLFPGGIVTEGSAGSTAISLATVAPA 122
Query: 428 LGIPCIVVVPIHTALNKVNKCEQLGAKFSR 517
G C VV+P A+ K E LGA R
Sbjct: 123 YGCKCHVVIPDDAAIEKSQIIEALGASVER 152
>At5g54810.1 68418.m06827 tryptophan synthase, beta subunit 1 (TSB1)
identical to SP|P14671
Length = 470
Score = 35.9 bits (79), Expect = 0.023
Identities = 25/75 (33%), Positives = 35/75 (46%), Gaps = 1/75 (1%)
Frame = +2
Query: 227 TVHPRSHVRGLGMDIYFKQEFLQYTGSFK-ERGVRNALISLSDEQKKNGVIAASTGNHGA 403
T H R G G IY K+E L +TG+ K V AL++ KK + G HG
Sbjct: 138 TEHYRRE-NGEGPLIYLKREDLNHTGAHKINNAVAQALLA-KRLGKKRIIAETGAGQHGV 195
Query: 404 ALSYHSTQLGIPCIV 448
A + + G+ CI+
Sbjct: 196 ATATVCARFGLECII 210
>At4g27070.1 68417.m03892 tryptophan synthase, beta subunit 2 (TSB2)
identical to SP|25269
Length = 475
Score = 35.9 bits (79), Expect = 0.023
Identities = 25/75 (33%), Positives = 35/75 (46%), Gaps = 1/75 (1%)
Frame = +2
Query: 227 TVHPRSHVRGLGMDIYFKQEFLQYTGSFK-ERGVRNALISLSDEQKKNGVIAASTGNHGA 403
T H R G G IY K+E L +TG+ K V AL++ KK + G HG
Sbjct: 143 TEHYRRE-NGEGPLIYLKREDLNHTGAHKINNAVAQALLA-KRLGKKRIIAETGAGQHGV 200
Query: 404 ALSYHSTQLGIPCIV 448
A + + G+ CI+
Sbjct: 201 ATATVCARFGLQCII 215
>At1g52855.1 68414.m05976 expressed protein
Length = 68
Score = 33.5 bits (73), Expect = 0.12
Identities = 10/29 (34%), Positives = 19/29 (65%)
Frame = +1
Query: 331 CADIVE*RTKEKWCYSSVYWKSWCSIKLS 417
CA I + +T ++C+ +YW+SW ++ S
Sbjct: 30 CASIADMKTSTRFCFIPIYWRSWRAVVCS 58
>At5g28237.2 68418.m03423 tryptophan synthase, beta subunit,
putative similar to SP|P14671 Tryptophan synthase beta
chain 1, chloroplast precursor (EC 4.2.1.20)
{Arabidopsis thaliana}; contains Pfam profile PF00291:
Pyridoxal-phosphate dependent enzyme
Length = 372
Score = 32.3 bits (70), Expect = 0.29
Identities = 23/66 (34%), Positives = 34/66 (51%), Gaps = 3/66 (4%)
Frame = +2
Query: 260 GMDIYFKQEFLQYTGSFKERGVRNALIS--LSDEQKKNGVIAAS-TGNHGAALSYHSTQL 430
G +IY K+E L + GS K + NAL +S + V+AA+ G HG A + +L
Sbjct: 142 GPEIYLKREDLSHCGSHK---INNALAQAMISRRLGCSRVVAATGAGQHGVATAAACAKL 198
Query: 431 GIPCIV 448
+ C V
Sbjct: 199 SLECTV 204
>At5g28237.1 68418.m03422 tryptophan synthase, beta subunit,
putative similar to SP|P14671 Tryptophan synthase beta
chain 1, chloroplast precursor (EC 4.2.1.20)
{Arabidopsis thaliana}; contains Pfam profile PF00291:
Pyridoxal-phosphate dependent enzyme
Length = 465
Score = 32.3 bits (70), Expect = 0.29
Identities = 23/66 (34%), Positives = 34/66 (51%), Gaps = 3/66 (4%)
Frame = +2
Query: 260 GMDIYFKQEFLQYTGSFKERGVRNALIS--LSDEQKKNGVIAAS-TGNHGAALSYHSTQL 430
G +IY K+E L + GS K + NAL +S + V+AA+ G HG A + +L
Sbjct: 142 GPEIYLKREDLSHCGSHK---INNALAQAMISRRLGCSRVVAATGAGQHGVATAAACAKL 198
Query: 431 GIPCIV 448
+ C V
Sbjct: 199 SLECTV 204
>At4g08450.1 68417.m01393 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1234
Score = 29.9 bits (64), Expect = 1.5
Identities = 17/66 (25%), Positives = 30/66 (45%)
Frame = +3
Query: 63 LKHVFIMKIYS*NLQIKMSVDIEFDENCDPNNPRKIKYDDILAASRRIVGAVVRTPCTRA 242
+ H++ + + S +L IKM F ++ PN ++ YD + A +G + R
Sbjct: 337 IDHIYEVLLPSKDLAIKMFCRSAFRKDSPPNGFIELAYDVVKRAGSLPLGLNILGSYLRG 396
Query: 243 HMSEDW 260
EDW
Sbjct: 397 RSKEDW 402
>At5g64600.1 68418.m08118 expressed protein similar to axi 1
[Nicotiana tabacum] GI:559921; contains Pfam profile
PF03138: Plant protein family
Length = 522
Score = 28.3 bits (60), Expect = 4.7
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = -2
Query: 179 IIFYFAWIIWITVFIKFNIDTHFD 108
+IF FA+ IW+ VF NI T D
Sbjct: 31 LIFVFAFTIWVLVFSSRNIQTDDD 54
>At3g46420.1 68416.m05032 leucine-rich repeat family protein /
protein kinase family protein contains leucine rich
repeat (LRR) domains, INTERPRO:IPR001611; contains
serine/threonine protein kinases active-site signature,
Prosite:PS00108
Length = 838
Score = 27.9 bits (59), Expect = 6.1
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = -1
Query: 303 PVYCKNSCLK*ISIPSPLTCERGCTVYELL 214
PVY + K I PSP+TCERG + E++
Sbjct: 309 PVYLQT---KTIRNPSPVTCERGECILEMI 335
>At2g03780.1 68415.m00338 translin family protein similar to
SP|Q99598 Translin-associated protein X
(Translin-associated factor X) {Homo sapiens}; contains
Pfam profile PF01997: Translin family
Length = 287
Score = 27.9 bits (59), Expect = 6.1
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +1
Query: 292 TVYWKFQRTWSPKCADIVE*RTKEKWCYS 378
T +WK +R +SP + VE T K+C S
Sbjct: 126 TDFWKLRRAYSPGVQEYVEAATFYKFCLS 154
>At1g09040.1 68414.m01008 expressed protein
Length = 911
Score = 27.9 bits (59), Expect = 6.1
Identities = 18/64 (28%), Positives = 31/64 (48%), Gaps = 2/64 (3%)
Frame = +2
Query: 338 ISLSDEQKKNGVIAASTGNHGAALS--YHSTQLGIPCIVVVPIHTALNKVNKCEQLGAKF 511
IS S EQK NG+ + S + A + L +P I + ++ N + ++LG+
Sbjct: 710 ISTSHEQKPNGLCSVSKSDKKRASNDLEQKQALELPSISGLNNRSSSNDLGTTQELGSSE 769
Query: 512 SRHG 523
+HG
Sbjct: 770 QQHG 773
>At2g47070.1 68415.m05881 squamosa promoter-binding protein-like 1
(SPL1) identical to squamosa promoter binding
protein-like 1 [Arabidopsis thaliana] GI:5931655;
contains Pfam profile PF03110: SBP domain
Length = 881
Score = 27.5 bits (58), Expect = 8.1
Identities = 17/60 (28%), Positives = 31/60 (51%)
Frame = +2
Query: 185 IGSFKTNCRGSSSYTVHPRSHVRGLGMDIYFKQEFLQYTGSFKERGVRNALISLSDEQKK 364
I ++KT CR S+ +T + +RG I+ Q + + ++ V N +S SD ++K
Sbjct: 743 IEAWKT-CRDSTGFTPEDYARLRGHFSYIHLIQRKINKKSTTEDHVVVNIPVSFSDREQK 801
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,117,183
Number of Sequences: 28952
Number of extensions: 299586
Number of successful extensions: 728
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 706
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 723
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -