BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesS0601 (548 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_56046| Best HMM Match : No HMM Matches (HMM E-Value=.) 57 1e-08 SB_17112| Best HMM Match : Trypsin (HMM E-Value=0) 33 0.20 >SB_56046| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 674 Score = 56.8 bits (131), Expect = 1e-08 Identities = 40/122 (32%), Positives = 60/122 (49%), Gaps = 5/122 (4%) Frame = +2 Query: 158 LRGIDHIKDPRLNKGLAFTLEEHKL*VFTDFWP-----QI*NPGRTVGVLPNFIR*I*RR 322 +RG D ++D LNKGLAFTLEE ++ P Q R L + + Sbjct: 60 IRGTDIMRDSHLNKGLAFTLEERQILGIHGLLPPCVISQEIQAQRVYRELQRKPNDLEKY 119 Query: 323 FE*IPLFD*VAR*E*KTIFQFT*L*HRXIHAIVYTPTVGLACQKFGLVYRRPERIIYHYS 502 + + L + + +F +T + IVYTPTVGLAC+K+G+++RRP + Sbjct: 120 IQLMALLERNESLFFRVLFDYT----EELMPIVYTPTVGLACRKYGMIFRRPRGLFISIH 175 Query: 503 DK 508 DK Sbjct: 176 DK 177 Score = 29.1 bits (62), Expect = 2.5 Identities = 19/56 (33%), Positives = 26/56 (46%) Frame = +3 Query: 351 LQDRNEKLFFSLLDCDIXKFMPLCTRLRLDWRARNLVSFTGDPRG*YITILIKTHI 518 L +RNE LFF +L + MP+ + R PRG +I+I K HI Sbjct: 125 LLERNESLFFRVLFDYTEELMPIVYTPTVGLACRKYGMIFRRPRGLFISIHDKGHI 180 >SB_17112| Best HMM Match : Trypsin (HMM E-Value=0) Length = 636 Score = 32.7 bits (71), Expect = 0.20 Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 4/42 (9%) Frame = +2 Query: 116 NMHEITGNIICPNML----RGIDHIKDPRLNKGLAFTLEEHK 229 N H G ++ P + +DH+KDP+ LA TL EHK Sbjct: 373 NGHHCGGTLVSPQWVVTAAHCVDHVKDPKNYNELAITLGEHK 414 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,304,938 Number of Sequences: 59808 Number of extensions: 294527 Number of successful extensions: 484 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 465 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 484 length of database: 16,821,457 effective HSP length: 78 effective length of database: 12,156,433 effective search space used: 1264269032 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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