Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesS0487
(642 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 51 2e-07
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 47 2e-06
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 47 3e-06
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 44 1e-05
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 44 2e-05
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 44 2e-05
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 42 8e-05
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 42 8e-05
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 41 1e-04
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 38 0.001
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 37 0.003
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 35 0.011
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 26 5.3
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 50.8 bits (116), Expect = 2e-07
Identities = 26/66 (39%), Positives = 34/66 (51%)
Frame = +2
Query: 305 RRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKFTNKYP 484
+RL+ ELMSLM PG++ D S NL W + G T YEG KF + F YP
Sbjct: 31 KRLRSELMSLMMSNTPGISAFPDSDS-NLLHWAGTITGPSDTYYEGLKFKISMSFPANYP 89
Query: 485 FDSPEV 502
+ P +
Sbjct: 90 YSPPTI 95
Score = 38.3 bits (85), Expect = 0.001
Identities = 15/33 (45%), Positives = 23/33 (69%)
Frame = +1
Query: 529 HPHVYSNGHICLSXLTDDWSPALSVQSVCLSIR 627
HP+V +G+ICL L D WS +VQ++ LS++
Sbjct: 103 HPNVDMSGNICLDILKDKWSAVYNVQTILLSLQ 135
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 47.2 bits (107), Expect = 2e-06
Identities = 23/66 (34%), Positives = 37/66 (56%)
Frame = +2
Query: 305 RRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKFTNKYP 484
+R+ KE+ +L ++PPPG+ + NL + + MEG + YEG KF L+ ++YP
Sbjct: 5 KRIIKEIETLTRDPPPGIVAAPTE--DNLRYFKITMEGPQQSAYEGGKFHLELFLPDEYP 62
Query: 485 FDSPEV 502
P V
Sbjct: 63 MMPPNV 68
Score = 44.0 bits (99), Expect = 2e-05
Identities = 19/33 (57%), Positives = 24/33 (72%)
Frame = +1
Query: 529 HPHVYSNGHICLSXLTDDWSPALSVQSVCLSIR 627
HP+V G ICLS L DWSPAL +++V LSI+
Sbjct: 76 HPNVDKLGRICLSTLKKDWSPALQIRTVLLSIQ 108
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 46.8 bits (106), Expect = 3e-06
Identities = 19/32 (59%), Positives = 23/32 (71%)
Frame = +1
Query: 529 HPHVYSNGHICLSXLTDDWSPALSVQSVCLSI 624
HP++ SNG ICL L D WSPAL++ V LSI
Sbjct: 75 HPNINSNGSICLDILRDQWSPALTISKVLLSI 106
Score = 37.1 bits (82), Expect = 0.002
Identities = 20/66 (30%), Positives = 29/66 (43%)
Frame = +2
Query: 305 RRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKFTNKYP 484
+R+ +EL L K+PP + +L W + G + Y G F L F YP
Sbjct: 4 KRINRELADLGKDPPSSCSAG--PVGDDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYP 61
Query: 485 FDSPEV 502
F P+V
Sbjct: 62 FKPPKV 67
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 44.4 bits (100), Expect = 1e-05
Identities = 26/75 (34%), Positives = 33/75 (44%)
Frame = +2
Query: 278 MYYTSVNSQRRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVL 457
M S +S RRL KE L + P P + V+ NL W G ++Y G KF
Sbjct: 1 MASASPSSSRRLTKEYSDLREHPIPDIRVN--LVDDNLFHWACTALGPSDSVYAGGKFHF 58
Query: 458 QFKFTNKYPFDSPEV 502
KF YPF P +
Sbjct: 59 SLKFPLDYPFQPPTI 73
Score = 25.0 bits (52), Expect = 9.3
Identities = 10/29 (34%), Positives = 19/29 (65%), Gaps = 1/29 (3%)
Frame = +1
Query: 529 HPHVYSNGHICLSXLTDD-WSPALSVQSV 612
HP+ S G++CL+ L + P++ ++SV
Sbjct: 81 HPNFDSEGNVCLAILKQQVFKPSIKLRSV 109
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 44.0 bits (99), Expect = 2e-05
Identities = 21/56 (37%), Positives = 34/56 (60%), Gaps = 1/56 (1%)
Frame = +1
Query: 460 VQIHKQIPL*FP*SNVLGNNIPVHPHV-YSNGHICLSXLTDDWSPALSVQSVCLSI 624
+ +H+ P+ P +V VHP++ ++NG +C+ L WSPA S+QS CL+I
Sbjct: 58 IHVHEGYPISPP--SVYFQTKIVHPNISWTNGEVCMDILKTHWSPAWSLQSACLAI 111
Score = 32.7 bits (71), Expect = 0.046
Identities = 20/69 (28%), Positives = 29/69 (42%)
Frame = +2
Query: 296 NSQRRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKFTN 475
+S +RL KE S G+ + ++ W +EG T YEG ++VL
Sbjct: 3 SSIKRLHKEYASFQAGDISGLLLLKPVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHVHE 62
Query: 476 KYPFDSPEV 502
YP P V
Sbjct: 63 GYPISPPSV 71
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 43.6 bits (98), Expect = 2e-05
Identities = 24/71 (33%), Positives = 35/71 (49%)
Frame = +2
Query: 290 SVNSQRRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKF 469
S + +RL KE ++L K P VD A++N+ W + G P T YEG ++ F
Sbjct: 3 SKGAYKRLMKEYLALQKNPVE--LVDAKPATENILEWHYIITGPPDTPYEGGQYHGTLIF 60
Query: 470 TNKYPFDSPEV 502
YPF P +
Sbjct: 61 PPDYPFKPPAI 71
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 41.9 bits (94), Expect = 8e-05
Identities = 22/71 (30%), Positives = 34/71 (47%)
Frame = +2
Query: 290 SVNSQRRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKF 469
S ++RRL ++ + ++PP GV+ S N+ LW + G T +E F L F
Sbjct: 2 STTARRRLMRDFKRMQQDPPAGVSAS--PVSDNVMLWNAVIIGPADTPFEDGTFKLVLSF 59
Query: 470 TNKYPFDSPEV 502
+YP P V
Sbjct: 60 DEQYPNKPPLV 70
Score = 40.7 bits (91), Expect = 2e-04
Identities = 15/33 (45%), Positives = 22/33 (66%)
Frame = +1
Query: 529 HPHVYSNGHICLSXLTDDWSPALSVQSVCLSIR 627
HP+VY+NG +CL L + WSP V ++ SI+
Sbjct: 78 HPNVYANGELCLDILQNRWSPTYDVAAILTSIQ 110
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 41.9 bits (94), Expect = 8e-05
Identities = 23/68 (33%), Positives = 35/68 (51%)
Frame = +2
Query: 299 SQRRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKFTNK 478
S++ L+K+L + K PP G +V G +++ W V + G TLYEG F F
Sbjct: 6 SEQLLRKQLKEIQKNPPQGFSV-GLVDDKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQD 64
Query: 479 YPFDSPEV 502
YP P++
Sbjct: 65 YPLMPPKM 72
Score = 25.8 bits (54), Expect = 5.3
Identities = 8/15 (53%), Positives = 12/15 (80%)
Frame = +1
Query: 529 HPHVYSNGHICLSXL 573
HP+V+ NG +C+S L
Sbjct: 80 HPNVHPNGEVCISIL 94
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 41.1 bits (92), Expect = 1e-04
Identities = 21/64 (32%), Positives = 29/64 (45%)
Frame = +2
Query: 305 RRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKFTNKYP 484
RRL KE L + P G+T G + W ++G GT +EG + KF + YP
Sbjct: 8 RRLMKEYKELTENGPDGITA-GPSNEDDFFTWDCLIQGPDGTPFEGGLYPATLKFPSDYP 66
Query: 485 FDSP 496
P
Sbjct: 67 LGPP 70
Score = 29.1 bits (62), Expect = 0.57
Identities = 15/45 (33%), Positives = 24/45 (53%), Gaps = 13/45 (28%)
Frame = +1
Query: 529 HPHVYSNGHICLSXL-------------TDDWSPALSVQSVCLSI 624
HP+VY +G +C+S L ++ WSP SV+ + LS+
Sbjct: 80 HPNVYKDGTVCISILHAPGDDPNMYESSSERWSPVQSVEKILLSV 124
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 38.3 bits (85), Expect = 0.001
Identities = 14/35 (40%), Positives = 25/35 (71%), Gaps = 2/35 (5%)
Frame = +1
Query: 529 HPHVYSNGHICLSXLTDD--WSPALSVQSVCLSIR 627
HP+VY +G +CLS L ++ W PA++++ + L I+
Sbjct: 83 HPNVYPSGTVCLSILNEEEGWKPAITIKQILLGIQ 117
Score = 31.1 bits (67), Expect = 0.14
Identities = 20/67 (29%), Positives = 31/67 (46%), Gaps = 3/67 (4%)
Frame = +2
Query: 308 RLQKELMSLMKEPPPGVTVDGDQASQ---NLTLWTVHMEGVPGTLYEGEKFVLQFKFTNK 478
RLQ+E ++ P G ++S +L W V + G P T +EG + L F +
Sbjct: 8 RLQEERKQWRRDHPFGFYAKPCKSSDGGLDLMNWKVGIPGKPKTSWEGGLYKLTMAFPEE 67
Query: 479 YPFDSPE 499
YP P+
Sbjct: 68 YPTRPPK 74
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 36.7 bits (81), Expect = 0.003
Identities = 11/33 (33%), Positives = 23/33 (69%)
Frame = +1
Query: 529 HPHVYSNGHICLSXLTDDWSPALSVQSVCLSIR 627
HP++ G++CL+ L DW+P L++ S+ + ++
Sbjct: 95 HPNIDIEGNVCLNILRQDWNPVLNLNSILVGLQ 127
Score = 27.5 bits (58), Expect = 1.7
Identities = 20/69 (28%), Positives = 34/69 (49%), Gaps = 1/69 (1%)
Frame = +2
Query: 299 SQRRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGT-LYEGEKFVLQFKFTN 475
+Q R+QK++ L E P ++ + L +H+E P Y+G KF + + +
Sbjct: 24 AQIRIQKDVTDL--EIPSTMSTSWPDP---IKLNVLHLEIRPDEGYYKGGKFKFRIQIDD 78
Query: 476 KYPFDSPEV 502
YP D P+V
Sbjct: 79 NYPHDPPKV 87
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 34.7 bits (76), Expect = 0.011
Identities = 21/71 (29%), Positives = 34/71 (47%)
Frame = +2
Query: 290 SVNSQRRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKF 469
S N RR+ KEL + ++ G+ V + +++ G GT YEG FV+ +
Sbjct: 2 SDNRSRRIAKELADVQQDKQAGIQVW--TINDDISHLKGMFRGPEGTPYEGGYFVVDIEI 59
Query: 470 TNKYPFDSPEV 502
YPF P++
Sbjct: 60 PIDYPFRPPKM 70
Score = 34.7 bits (76), Expect = 0.011
Identities = 15/34 (44%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
Frame = +1
Query: 529 HPHVYSN-GHICLSXLTDDWSPALSVQSVCLSIR 627
HP+V S G ICL L D WSP +++S +S++
Sbjct: 78 HPNVSSQTGAICLDILKDQWSPVYTMKSALISLQ 111
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 25.8 bits (54), Expect = 5.3
Identities = 17/67 (25%), Positives = 33/67 (49%)
Frame = +2
Query: 302 QRRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKFTNKY 481
+RR++ ++M L+ VT+ D N+ + V G T Y G + + + ++Y
Sbjct: 5 RRRIETDVMKLLMSDYE-VTLVND----NMQEFYVRFHGPSETPYSGGIWKVHVELPSEY 59
Query: 482 PFDSPEV 502
P+ SP +
Sbjct: 60 PWKSPSI 66
Score = 25.4 bits (53), Expect = 7.0
Identities = 14/53 (26%), Positives = 28/53 (52%), Gaps = 3/53 (5%)
Frame = +1
Query: 463 QIHKQIPL*FP*-SNVLGN-NIPVHPHVYS-NGHICLSXLTDDWSPALSVQSV 612
++H ++P +P S +G N HP++ +G +CL + WSP + ++
Sbjct: 50 KVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLDVINQTWSPMFDMINI 102
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,559,141
Number of Sequences: 5004
Number of extensions: 52806
Number of successful extensions: 139
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 118
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 287744314
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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