Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesS0441
(598 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_UPI00015B4325 Cluster: PREDICTED: similar to aconitase,... 178 9e-44
UniRef50_Q99798 Cluster: Aconitate hydratase, mitochondrial prec... 176 3e-43
UniRef50_Q9VIE8 Cluster: CG9244-PB; n=37; cellular organisms|Rep... 171 1e-41
UniRef50_Q0CSK9 Cluster: Aconitate hydratase, mitochondrial; n=2... 161 9e-39
UniRef50_P19414 Cluster: Aconitate hydratase, mitochondrial prec... 155 1e-36
UniRef50_O13966 Cluster: Aconitate hydratase, mitochondrial prec... 143 3e-33
UniRef50_Q2UTF0 Cluster: Aconitase/homoaconitase; n=9; cellular ... 115 1e-24
UniRef50_Q4PB22 Cluster: Putative uncharacterized protein; n=5; ... 113 4e-24
UniRef50_Q4AHJ7 Cluster: Aconitate hydratase; n=1; Chlorobium ph... 106 3e-22
UniRef50_Q0USA6 Cluster: Putative uncharacterized protein; n=2; ... 105 6e-22
UniRef50_A0RTP9 Cluster: 3-isopropylmalate isomerase/aconitase A... 96 6e-19
UniRef50_Q5AVN8 Cluster: Putative uncharacterized protein; n=1; ... 57 4e-07
UniRef50_Q5A379 Cluster: Putative uncharacterized protein; n=1; ... 44 0.004
UniRef50_Q74AD1 Cluster: Aconitate hydratase, putative; n=12; Ba... 38 0.24
UniRef50_Q7NDZ5 Cluster: Aconitate hydratase; n=28; Bacteria|Rep... 36 0.72
UniRef50_Q07RK3 Cluster: DNA or RNA helicase of superfamily II; ... 35 1.7
>UniRef50_UPI00015B4325 Cluster: PREDICTED: similar to aconitase,
mitochondrial; n=1; Nasonia vitripennis|Rep: PREDICTED:
similar to aconitase, mitochondrial - Nasonia
vitripennis
Length = 917
Score = 178 bits (433), Expect = 9e-44
Identities = 84/107 (78%), Positives = 92/107 (85%)
Frame = +2
Query: 257 LTLSEKILYSHLDDPKGQEIERGASYLRLXPDRVAMQDATAQMAMLQFISSGLPRXAVPS 436
LTLSEKILYSHLD+P+ Q+I RG SYLRL PDRVAMQDATAQMAMLQFISSGLPR AVPS
Sbjct: 197 LTLSEKILYSHLDEPQKQDIVRGTSYLRLRPDRVAMQDATAQMAMLQFISSGLPRVAVPS 256
Query: 437 TIHCDHLIEAQVGGEKDLARAKDLTKKYTSF*RLLGAKYGXGFWKXG 577
TIHCDHLIEAQ+GG+KDL RAKD+ K+ SF + GAKYG GFW G
Sbjct: 257 TIHCDHLIEAQLGGDKDLKRAKDINKEVYSFLKTAGAKYGVGFWNPG 303
Score = 64.5 bits (150), Expect = 2e-09
Identities = 31/49 (63%), Positives = 39/49 (79%), Gaps = 1/49 (2%)
Frame = +3
Query: 111 SEIQQRCFSVSPLTAAAAQVAMSKFDKVP-LXYEKLTKNLEVVXKRLGR 254
+E+QQRCFS SPLT AAA+VAMSKFD L Y+KL +N+++V KRL R
Sbjct: 147 AEVQQRCFSTSPLTFAAAKVAMSKFDSTAYLPYDKLDENIKIVKKRLDR 195
>UniRef50_Q99798 Cluster: Aconitate hydratase, mitochondrial
precursor; n=28; cellular organisms|Rep: Aconitate
hydratase, mitochondrial precursor - Homo sapiens
(Human)
Length = 780
Score = 176 bits (429), Expect = 3e-43
Identities = 84/107 (78%), Positives = 90/107 (84%)
Frame = +2
Query: 257 LTLSEKILYSHLDDPKGQEIERGASYLRLXPDRVAMQDATAQMAMLQFISSGLPRXAVPS 436
LTLSEKI+Y HLDDP QEIERG SYLRL PDRVAMQDATAQMAMLQFISSGL + AVPS
Sbjct: 63 LTLSEKIVYGHLDDPASQEIERGKSYLRLRPDRVAMQDATAQMAMLQFISSGLSKVAVPS 122
Query: 437 TIHCDHLIEAQVGGEKDLARAKDLTKKYTSF*RLLGAKYGXGFWKXG 577
TIHCDHLIEAQVGGEKDL RAKD+ ++ +F GAKYG GFWK G
Sbjct: 123 TIHCDHLIEAQVGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWKPG 169
>UniRef50_Q9VIE8 Cluster: CG9244-PB; n=37; cellular organisms|Rep:
CG9244-PB - Drosophila melanogaster (Fruit fly)
Length = 787
Score = 171 bits (415), Expect = 1e-41
Identities = 81/107 (75%), Positives = 88/107 (82%)
Frame = +2
Query: 257 LTLSEKILYSHLDDPKGQEIERGASYLRLXPDRVAMQDATAQMAMLQFISSGLPRXAVPS 436
LTLSEK+LYSHLDDP Q+I RG SYLRL PDRVAMQDATAQMA+LQFISSGL + AVPS
Sbjct: 70 LTLSEKVLYSHLDDPANQDIVRGTSYLRLRPDRVAMQDATAQMALLQFISSGLKKVAVPS 129
Query: 437 TIHCDHLIEAQVGGEKDLARAKDLTKKYTSF*RLLGAKYGXGFWKXG 577
T+HCDHLIEAQ+GG KDLARAKDL K+ F AKYG GFWK G
Sbjct: 130 TVHCDHLIEAQIGGPKDLARAKDLNKEVYDFLASTCAKYGLGFWKPG 176
Score = 37.9 bits (84), Expect = 0.18
Identities = 21/33 (63%), Positives = 24/33 (72%), Gaps = 1/33 (3%)
Frame = +3
Query: 159 AAQVAMSKFDK-VPLXYEKLTKNLEVVXKRLGR 254
A++VA+SKFD V L YEKL K LEVV RL R
Sbjct: 36 ASKVALSKFDSDVYLPYEKLNKRLEVVRGRLNR 68
>UniRef50_Q0CSK9 Cluster: Aconitate hydratase, mitochondrial; n=2;
Pezizomycotina|Rep: Aconitate hydratase, mitochondrial -
Aspergillus terreus (strain NIH 2624)
Length = 781
Score = 161 bits (392), Expect = 9e-39
Identities = 73/107 (68%), Positives = 86/107 (80%)
Frame = +2
Query: 257 LTLSEKILYSHLDDPKGQEIERGASYLRLXPDRVAMQDATAQMAMLQFISSGLPRXAVPS 436
LT +EK+LYSHLDDP GQ+IERG SYL+L PDRVA QDATAQMA+LQF+S+G+P A P+
Sbjct: 93 LTYAEKVLYSHLDDPHGQDIERGVSYLKLRPDRVACQDATAQMAILQFMSAGMPSVATPT 152
Query: 437 TIHCDHLIEAQVGGEKDLARAKDLTKKYTSF*RLLGAKYGXGFWKXG 577
T+HCDHLIEAQVGGEKDLARA ++ K+ F AKY GFWK G
Sbjct: 153 TVHCDHLIEAQVGGEKDLARANEINKEVYDFLATATAKYNIGFWKPG 199
>UniRef50_P19414 Cluster: Aconitate hydratase, mitochondrial
precursor; n=41; cellular organisms|Rep: Aconitate
hydratase, mitochondrial precursor - Saccharomyces
cerevisiae (Baker's yeast)
Length = 778
Score = 155 bits (375), Expect = 1e-36
Identities = 72/106 (67%), Positives = 83/106 (78%)
Frame = +2
Query: 260 TLSEKILYSHLDDPKGQEIERGASYLRLXPDRVAMQDATAQMAMLQFISSGLPRXAVPST 439
T +EKILY HLDDP GQ+I+RG SYL+L PDRVA QDATAQMA+LQF+S+GLP+ A P T
Sbjct: 60 TYAEKILYGHLDDPHGQDIQRGVSYLKLRPDRVACQDATAQMAILQFMSAGLPQVAKPVT 119
Query: 440 IHCDHLIEAQVGGEKDLARAKDLTKKYTSF*RLLGAKYGXGFWKXG 577
+HCDHLI+AQVGGEKDL RA DL K+ F AKY GFWK G
Sbjct: 120 VHCDHLIQAQVGGEKDLKRAIDLNKEVYDFLASATAKYNMGFWKPG 165
>UniRef50_O13966 Cluster: Aconitate hydratase, mitochondrial
precursor; n=21; cellular organisms|Rep: Aconitate
hydratase, mitochondrial precursor - Schizosaccharomyces
pombe (Fission yeast)
Length = 778
Score = 143 bits (346), Expect = 3e-33
Identities = 68/107 (63%), Positives = 78/107 (72%)
Frame = +2
Query: 257 LTLSEKILYSHLDDPKGQEIERGASYLRLXPDRVAMQDATAQMAMLQFISSGLPRXAVPS 436
LT SEKILY HLDDP Q+IERG SYL+L PDRVA QDATAQMA+LQF+S+G+P AVP
Sbjct: 59 LTYSEKILYGHLDDPVNQDIERGVSYLKLRPDRVACQDATAQMAILQFMSAGMPEVAVPV 118
Query: 437 TIHCDHLIEAQVGGEKDLARAKDLTKKYTSF*RLLGAKYGXGFWKXG 577
T+HCDHLIEA GG DL RA K+ F + AKY GFW+ G
Sbjct: 119 TVHCDHLIEAYEGGPIDLERANVTNKEVYDFLQTACAKYNIGFWRPG 165
Score = 37.5 bits (83), Expect = 0.24
Identities = 21/53 (39%), Positives = 33/53 (62%), Gaps = 3/53 (5%)
Frame = +3
Query: 105 VLSEIQQRCFSVSPLTAA--AAQVAMSKFDKVP-LXYEKLTKNLEVVXKRLGR 254
+ ++ R FS +P+ A A +VAMS F+K + Y+++ NLE+V KRL R
Sbjct: 5 IFTQSTLRSFSCAPVAANIDAKKVAMSNFEKNKFINYQRIKDNLEIVKKRLNR 57
>UniRef50_Q2UTF0 Cluster: Aconitase/homoaconitase; n=9; cellular
organisms|Rep: Aconitase/homoaconitase - Aspergillus
oryzae
Length = 806
Score = 115 bits (276), Expect = 1e-24
Identities = 62/112 (55%), Positives = 74/112 (66%), Gaps = 5/112 (4%)
Frame = +2
Query: 257 LTLSEKILYSHL---DDPKG--QEIERGASYLRLXPDRVAMQDATAQMAMLQFISSGLPR 421
LTL+EK+LYSHL DD QEI+RG + L L PDRVA DATA MA+LQFIS+GLPR
Sbjct: 67 LTLTEKLLYSHLIPSDDKVWSLQEIDRGKTILELRPDRVACHDATATMALLQFISAGLPR 126
Query: 422 XAVPSTIHCDHLIEAQVGGEKDLARAKDLTKKYTSF*RLLGAKYGXGFWKXG 577
AVP+T+H DHLI ++ G E D+ RA + F KYG GFWK G
Sbjct: 127 VAVPTTVHGDHLIVSEKGAEPDMKRALTEHAEVYEFLSSASRKYGIGFWKPG 178
>UniRef50_Q4PB22 Cluster: Putative uncharacterized protein; n=5;
Fungi/Metazoa group|Rep: Putative uncharacterized
protein - Ustilago maydis (Smut fungus)
Length = 1041
Score = 113 bits (271), Expect = 4e-24
Identities = 59/115 (51%), Positives = 77/115 (66%), Gaps = 8/115 (6%)
Frame = +2
Query: 257 LTLSEKILYSHLDDPK------GQEIE--RGASYLRLXPDRVAMQDATAQMAMLQFISSG 412
LTLSEKILYSHL +P+ G ++ RG YL+L DR+AMQDA+AQMA+LQF++ G
Sbjct: 171 LTLSEKILYSHLRNPEHDLAGVGADVSAIRGKKYLKLKIDRLAMQDASAQMALLQFMTCG 230
Query: 413 LPRXAVPSTIHCDHLIEAQVGGEKDLARAKDLTKKYTSF*RLLGAKYGXGFWKXG 577
LPR A+PS++HCDHLI+A G E DL R+ ++ +F KYG FW G
Sbjct: 231 LPRTAIPSSVHCDHLIQAFEGAEADLKRSIASNQEVFAFLESASKKYGIEFWGPG 285
>UniRef50_Q4AHJ7 Cluster: Aconitate hydratase; n=1; Chlorobium
phaeobacteroides BS1|Rep: Aconitate hydratase -
Chlorobium phaeobacteroides BS1
Length = 141
Score = 106 bits (255), Expect = 3e-22
Identities = 52/88 (59%), Positives = 67/88 (76%), Gaps = 1/88 (1%)
Frame = +2
Query: 257 LTLSEKILYSHLD-DPKGQEIERGASYLRLXPDRVAMQDATAQMAMLQFISSGLPRXAVP 433
+TL+EKILY+HLD + +RG SY+ PDRVAMQDATAQMA+LQF+ +G P+ AV
Sbjct: 43 MTLAEKILYAHLDGELPSASFDRGNSYVDFRPDRVAMQDATAQMALLQFMQAGKPQAAVS 102
Query: 434 STIHCDHLIEAQVGGEKDLARAKDLTKK 517
S++HCDHLI+A+ G E+DLA A D T K
Sbjct: 103 SSVHCDHLIQAKSGAEQDLANA-DFTNK 129
>UniRef50_Q0USA6 Cluster: Putative uncharacterized protein; n=2;
Pezizomycotina|Rep: Putative uncharacterized protein -
Phaeosphaeria nodorum (Septoria nodorum)
Length = 781
Score = 105 bits (253), Expect = 6e-22
Identities = 56/116 (48%), Positives = 77/116 (66%), Gaps = 9/116 (7%)
Frame = +2
Query: 257 LTLSEKILYSHLDDPK---------GQEIERGASYLRLXPDRVAMQDATAQMAMLQFISS 409
LTL+EKILY+HL++P+ G++I RG + L+L PDRVAMQDA+AQMA+LQF+S
Sbjct: 71 LTLAEKILYAHLENPEESLLSNTNNGRDI-RGQANLKLKPDRVAMQDASAQMALLQFMSC 129
Query: 410 GLPRXAVPSTIHCDHLIEAQVGGEKDLARAKDLTKKYTSF*RLLGAKYGXGFWKXG 577
GL + AVP++IHCDH+I + G + DL ++ K+ F KYG FW G
Sbjct: 130 GLGKTAVPASIHCDHMIVGEKGADLDLPQSIKGNKEVFDFLESAAKKYGIEFWPPG 185
>UniRef50_A0RTP9 Cluster: 3-isopropylmalate isomerase/aconitase A;
n=7; cellular organisms|Rep: 3-isopropylmalate
isomerase/aconitase A - Cenarchaeum symbiosum
Length = 754
Score = 95.9 bits (228), Expect = 6e-19
Identities = 48/107 (44%), Positives = 63/107 (58%)
Frame = +2
Query: 257 LTLSEKILYSHLDDPKGQEIERGASYLRLXPDRVAMQDATAQMAMLQFISSGLPRXAVPS 436
LTLSEKI+ H+ Y+ L PDRVA+QD T QM MLQF+ + A+P+
Sbjct: 32 LTLSEKIMAGHMVRDGTDIPVENKDYVHLTPDRVALQDVTGQMVMLQFMVTRHEETALPT 91
Query: 437 TIHCDHLIEAQVGGEKDLARAKDLTKKYTSF*RLLGAKYGXGFWKXG 577
TIHCDHLI A+V G +D+ + D + F + A+YG GFWK G
Sbjct: 92 TIHCDHLIRAKVEGGEDMRVSLDENSEVFRFLKSAAARYGCGFWKPG 138
>UniRef50_Q5AVN8 Cluster: Putative uncharacterized protein; n=1;
Emericella nidulans|Rep: Putative uncharacterized
protein - Emericella nidulans (Aspergillus nidulans)
Length = 326
Score = 56.8 bits (131), Expect = 4e-07
Identities = 27/47 (57%), Positives = 34/47 (72%)
Frame = +2
Query: 257 LTLSEKILYSHLDDPKGQEIERGASYLRLXPDRVAMQDATAQMAMLQ 397
LT +EK+LY+HLDD I RG + LR P R+A QDATAQMA++Q
Sbjct: 55 LTYAEKVLYNHLDDEFDGNIVRGQTQLRSKPVRIACQDATAQMALIQ 101
>UniRef50_Q5A379 Cluster: Putative uncharacterized protein; n=1;
Candida albicans|Rep: Putative uncharacterized protein -
Candida albicans (Yeast)
Length = 115
Score = 43.6 bits (98), Expect = 0.004
Identities = 27/103 (26%), Positives = 46/103 (44%)
Frame = -1
Query: 562 TXSIFSSEQSLKTCILLCEVLSPGQILLASDLGFDQVITMNGGRYSXTGKAGRDKL*HCH 383
T IF + + L +V GQI ++L DQ++T++ KL + H
Sbjct: 11 TQVIFGTSSGQEIVNFLVQVNGSGQIFWTTNLSLDQMVTVDSRWSGNLWNTSGHKLQNGH 70
Query: 382 LCSGVLHGHTVGXQAEITCAAFNFLSFGVIQVRI*DFLRQGQF 254
L +L + V Q ++ +FNFL +IQ+ + F Q+
Sbjct: 71 LGGSILTSNPVWSQFQVRDTSFNFLVMRIIQMTVQKFFSISQW 113
>UniRef50_Q74AD1 Cluster: Aconitate hydratase, putative; n=12;
Bacteria|Rep: Aconitate hydratase, putative - Geobacter
sulfurreducens
Length = 645
Score = 37.5 bits (83), Expect = 0.24
Identities = 27/65 (41%), Positives = 37/65 (56%), Gaps = 1/65 (1%)
Frame = +2
Query: 263 LSEKILYSHLDDPKGQEIERGASYLRLXPDRVAMQDATAQMAMLQFISSGLPRXAVP-ST 439
L+ KIL +HL KG E+ G + L D +QDAT MAML+FI+ G+ R V +
Sbjct: 5 LATKILEAHL--VKG-ELTPGTE-IALKIDHALLQDATGTMAMLEFIAMGVDRVKVELAA 60
Query: 440 IHCDH 454
+ DH
Sbjct: 61 QYIDH 65
>UniRef50_Q7NDZ5 Cluster: Aconitate hydratase; n=28; Bacteria|Rep:
Aconitate hydratase - Gloeobacter violaceus
Length = 645
Score = 35.9 bits (79), Expect = 0.72
Identities = 26/67 (38%), Positives = 35/67 (52%), Gaps = 1/67 (1%)
Frame = +2
Query: 257 LTLSEKILYSHLDDPKGQEIERGASYLRLXPDRVAMQDATAQMAMLQFISSGLPRXAVP- 433
+ L+ KIL +HL E+ G S + + D+ QDAT MA LQF + GLPR
Sbjct: 1 MNLTCKILQAHL---MSGELRPG-SEIGIRIDQTLTQDATGTMAYLQFEAMGLPRVRTKL 56
Query: 434 STIHCDH 454
S + DH
Sbjct: 57 SVSYIDH 63
>UniRef50_Q07RK3 Cluster: DNA or RNA helicase of superfamily II;
n=12; Bacteria|Rep: DNA or RNA helicase of superfamily
II - Rhodopseudomonas palustris (strain BisA53)
Length = 1066
Score = 34.7 bits (76), Expect = 1.7
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = -2
Query: 105 PPLSCYPXHAKLSYSAPWSPXFSILNELI*KNF 7
PP C P H ++ ++ W FS +N+L+ +N+
Sbjct: 12 PPADCMPRHLRVRLASAWGSRFSAMNQLLIQNY 44
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 575,969,250
Number of Sequences: 1657284
Number of extensions: 11093963
Number of successful extensions: 24896
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 24228
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24884
length of database: 575,637,011
effective HSP length: 97
effective length of database: 414,880,463
effective search space used: 41902926763
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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