BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesS0424 (698 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 156 2e-39 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 101 1e-22 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 100 2e-22 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 81 1e-16 SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 28 1.1 SPMIT.02 |||mitochondrial DNA binding endonuclease|Schizosacchar... 27 2.0 SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe... 27 2.0 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 3.4 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 3.4 SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 s... 26 4.5 SPAC25B8.10 |||trans-aconitate 3-methyltransferase |Schizosaccha... 26 4.5 SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|ch... 26 6.0 SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|ch... 26 6.0 SPBC2G2.01c |liz1|SPBC4B4.13c|pantothenate transporter |Schizosa... 25 7.9 SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomy... 25 7.9 SPAC24C9.14 |otu1|mug141|ubiquitin-specific protease |Schizosacc... 25 7.9 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 156 bits (379), Expect = 2e-39 Identities = 80/137 (58%), Positives = 92/137 (67%) Frame = +2 Query: 257 EASGGKYVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAEL 436 EA+GGKYVPRAV+VDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL Sbjct: 53 EAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAEL 112 Query: 437 VDSVLDVVRKEAEGCDCLQGFQLTILWEEVLDLVWGLF*FPKSVKSIQIVL*IRFPWYQV 616 D+VLDVVR+EAE CD LQGFQLT G K + + F Sbjct: 113 ADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPA 172 Query: 617 QXFPILWVEPYNATLSV 667 VEPYNATLS+ Sbjct: 173 PKSSDTVVEPYNATLSM 189 Score = 88.2 bits (209), Expect = 1e-18 Identities = 36/56 (64%), Positives = 44/56 (78%) Frame = +3 Query: 102 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLERINVYYTKLQG 269 MREIVHIQAGQCGNQ+GA FW I+DEHG+D+ G Y G S+ Q ER+NVY+ + G Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAG 56 Score = 34.7 bits (76), Expect = 0.013 Identities = 16/28 (57%), Positives = 18/28 (64%) Frame = +1 Query: 553 ISKIREEYPDRIMNTFXXXXXXXXXDTV 636 +SKIREEYPDR+M TF DTV Sbjct: 152 LSKIREEYPDRMMATFSVAPAPKSSDTV 179 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 101 bits (242), Expect = 1e-22 Identities = 51/136 (37%), Positives = 73/136 (53%) Frame = +2 Query: 257 EASGGKYVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAEL 436 E GKYVPR++ VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G EL Sbjct: 55 ETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKEL 114 Query: 437 VDSVLDVVRKEAEGCDCLQGFQLTILWEEVLDLVWGLF*FPKSVKSIQIVL*IRFPWYQV 616 VD V D +R+ A+ C LQGF + + +G + ++F Y Sbjct: 115 VDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPA 174 Query: 617 QXFPILWVEPYNATLS 664 VEPYN+ L+ Sbjct: 175 PQVSTSVVEPYNSVLT 190 Score = 40.7 bits (91), Expect = 2e-04 Identities = 18/34 (52%), Positives = 20/34 (58%) Frame = +3 Query: 102 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATG 203 MREI+ I GQ G QIG WE+ EHGI G Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNG 34 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 100 bits (239), Expect = 2e-22 Identities = 41/81 (50%), Positives = 58/81 (71%) Frame = +2 Query: 257 EASGGKYVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAEL 436 E GK+VPR++ VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E+ Sbjct: 59 ETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEM 118 Query: 437 VDSVLDVVRKEAEGCDCLQGF 499 +DSVL+ +R+ A+ C LQGF Sbjct: 119 IDSVLERIRRMADNCSGLQGF 139 Score = 38.7 bits (86), Expect = 8e-04 Identities = 17/42 (40%), Positives = 25/42 (59%) Frame = +3 Query: 102 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDL 227 MRE++ + GQ G QIG WE+ EHGI G + +S++ Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEV 42 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 81.4 bits (192), Expect = 1e-16 Identities = 31/86 (36%), Positives = 59/86 (68%), Gaps = 2/86 (2%) Frame = +2 Query: 254 YEASGGKYVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEG 427 Y++ +Y+PRA+++DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ Sbjct: 53 YQSDDTRYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHA 111 Query: 428 AELVDSVLDVVRKEAEGCDCLQGFQL 505 + + ++D++ +EA+G D L+GF L Sbjct: 112 ERIFEDIMDMIDREADGSDSLEGFSL 137 Score = 53.2 bits (122), Expect = 3e-08 Identities = 22/50 (44%), Positives = 34/50 (68%) Frame = +3 Query: 105 REIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLERINVYY 254 REI+ +QAGQCGNQIG++FW+ + EHGI G + ++R +V++ Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFF 52 >SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 28.3 bits (60), Expect = 1.1 Identities = 14/32 (43%), Positives = 17/32 (53%) Frame = -3 Query: 393 PAPDCPKTKLSGRKICPNGPERTESMVPGSRS 298 P+ PK L R I PNGPE + + GS S Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51 >SPMIT.02 |||mitochondrial DNA binding endonuclease|Schizosaccharomyces pombe|chr mitochondrial|||Manual Length = 384 Score = 27.5 bits (58), Expect = 2.0 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = +3 Query: 582 SYYEYVFRGTKSKXFRYCG*SHITQRYQFHQLVENTD 692 SY+E F+G KS F+ S + + Y F QL+ D Sbjct: 330 SYFENCFKGVKSLEFKIWSRS-LRKNYNFEQLLRARD 365 >SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 317 Score = 27.5 bits (58), Expect = 2.0 Identities = 17/64 (26%), Positives = 34/64 (53%) Frame = +1 Query: 97 QS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPTCSLNASMSTIRSFRGQV 276 +S S +S ++ +S SS S++S++ + ++A+P+ S ++S + SF Sbjct: 171 KSSSSSKSSSSSSSSSKSSSSSSSSSKSSSSSSSSSKSSASPSSSKSSSKFSSSSFITST 230 Query: 277 RPAS 288 PAS Sbjct: 231 TPAS 234 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 26.6 bits (56), Expect = 3.4 Identities = 15/43 (34%), Positives = 23/43 (53%) Frame = +2 Query: 404 AKGHYTEGAELVDSVLDVVRKEAEGCDCLQGFQLTILWEEVLD 532 A+GH G ELV + D +RK++E L+ I+ E L+ Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALEVCPDRIVCNEFLE 225 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 26.6 bits (56), Expect = 3.4 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +2 Query: 335 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 436 GP+G +F P F+F +G NW+ Y E A L Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190 >SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 subunit Alp6|Schizosaccharomyces pombe|chr 2|||Manual Length = 821 Score = 26.2 bits (55), Expect = 4.5 Identities = 10/35 (28%), Positives = 18/35 (51%) Frame = -1 Query: 170 YFPELCSDLIPALTRLDVDDFPHDCTKNLEPPESN 66 +F E+ + P +LD+DD + + P E+N Sbjct: 36 FFQEIIHSISPDTFQLDIDDILYKIYSKIPPEENN 70 >SPAC25B8.10 |||trans-aconitate 3-methyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 256 Score = 26.2 bits (55), Expect = 4.5 Identities = 14/37 (37%), Positives = 22/37 (59%), Gaps = 1/37 (2%) Frame = +2 Query: 431 ELVDSVLDVVRKEAEGCDCLQGFQLTI-LWEEVLDLV 538 ++ +LDV+RK+ DC G + I L +E +DLV Sbjct: 70 DVYPEMLDVLRKKFPNVDCRAGSAMAIPLEDESVDLV 106 >SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 25.8 bits (54), Expect = 6.0 Identities = 17/53 (32%), Positives = 26/53 (49%) Frame = +1 Query: 67 FDSGGSKFLVQS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPT 225 F S S S +STS RV++ ++ SSG + T+ + V +AT T Sbjct: 165 FVSPSSSSSSSSSAATSTSTRVSSSAKASTSSGAIAYTTKCVVVPVTASATAT 217 >SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 25.8 bits (54), Expect = 6.0 Identities = 17/53 (32%), Positives = 26/53 (49%) Frame = +1 Query: 67 FDSGGSKFLVQS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPT 225 F S S S +STS RV++ ++ SSG + T+ + V +AT T Sbjct: 165 FVSPSSSSSSSSSAATSTSTRVSSSAKASTSSGAIAYTTKCVVVPVTASATAT 217 >SPBC2G2.01c |liz1|SPBC4B4.13c|pantothenate transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 514 Score = 25.4 bits (53), Expect = 7.9 Identities = 11/31 (35%), Positives = 21/31 (67%), Gaps = 1/31 (3%) Frame = +2 Query: 512 LWEEVLDLVWGLF-*FPKSVKSIQIVL*IRF 601 LW V++++WGL F +V S++ ++ +RF Sbjct: 98 LWFSVMNILWGLMTIFSFAVHSVRALMILRF 128 >SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 25.4 bits (53), Expect = 7.9 Identities = 7/21 (33%), Positives = 14/21 (66%) Frame = -2 Query: 127 AWMWTISLMIALKIWNPPNRI 65 AW+W ++LM+ + PN++ Sbjct: 458 AWLWFMALMLLFPSYQNPNKV 478 >SPAC24C9.14 |otu1|mug141|ubiquitin-specific protease |Schizosaccharomyces pombe|chr 1|||Manual Length = 329 Score = 25.4 bits (53), Expect = 7.9 Identities = 9/21 (42%), Positives = 13/21 (61%) Frame = -1 Query: 443 NQPVQPPLCNVLSPSYSPPQT 381 N+P +PP+ N + PPQT Sbjct: 86 NEPAKPPIPNAATKPTFPPQT 106 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,028,672 Number of Sequences: 5004 Number of extensions: 64195 Number of successful extensions: 209 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 193 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 207 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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