SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= tesS0402
         (439 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr...   155   3e-39
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|...   109   2e-25
SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch...    48   6e-07
SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe...    45   6e-06
SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |...    28   0.72 
SPCC4F11.04c |||mannosyltransferase complex subunit |Schizosacch...    27   1.3  
SPBC1604.15 |gpi16||pig-T |Schizosaccharomyces pombe|chr 2|||Manual    27   1.3  
SPCPJ732.02c |||xylulose kinase |Schizosaccharomyces pombe|chr 3...    26   2.2  
SPCC1620.05 |||Rab geranylgeranyltransferase |Schizosaccharomyce...    25   3.9  
SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces pomb...    25   5.1  
SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|ch...    25   6.7  
SPBC16H5.12c |||conserved fungal protein|Schizosaccharomyces pom...    24   8.9  
SPAC222.11 |hem13||coproporphyrinogen III oxidase |Schizosacchar...    24   8.9  

>SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 591

 Score =  155 bits (376), Expect = 3e-39
 Identities = 68/86 (79%), Positives = 77/86 (89%)
 Frame = +1

Query: 1   RRFLDNMGFLEVETPLMNXVPGGATAKPFITHHNELNMDLYMRIAPELYHKMLVVGGLDR 180
           R F D+  F+EVETP+MN + GGATAKPF+THHN+L+MDLYMRIAPELY KMLVVGGLDR
Sbjct: 259 RHFFDSRDFMEVETPMMNMIAGGATAKPFVTHHNDLDMDLYMRIAPELYLKMLVVGGLDR 318

Query: 181 VYEIGRQFRNEGIDLTHNPEFTTCDF 258
           VYEIGRQFRNEG DLTHNPEFT+ +F
Sbjct: 319 VYEIGRQFRNEGADLTHNPEFTSIEF 344



 Score = 67.3 bits (157), Expect = 1e-12
 Identities = 29/45 (64%), Positives = 34/45 (75%)
 Frame = +3

Query: 255 FYMAYADYNDLMTITESILSGMVQSIHGSYKVKYHPEGPXGKEIE 389
           FY AYADY DLM  TE +LSG+V+ + GSYKV YHPEGP G + E
Sbjct: 344 FYQAYADYYDLMDTTEELLSGLVKDLTGSYKVPYHPEGPEGPKWE 388


>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 531

 Score =  109 bits (261), Expect = 2e-25
 Identities = 49/85 (57%), Positives = 65/85 (76%)
 Frame = +1

Query: 1   RRFLDNMGFLEVETPLMNXVPGGATAKPFITHHNELNMDLYMRIAPELYHKMLVVGGLDR 180
           R+F    GFLEVETP+++   GGATA+PFIT      + L +R APEL+ K LV+GG++R
Sbjct: 215 RKFFSERGFLEVETPILSHHFGGATARPFITSDIH-KLPLTLRCAPELWLKQLVIGGMNR 273

Query: 181 VYEIGRQFRNEGIDLTHNPEFTTCD 255
           V+E+G+ FRNEGID THNPEFT+C+
Sbjct: 274 VFELGKNFRNEGIDATHNPEFTSCE 298


>SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 611

 Score = 48.0 bits (109), Expect = 6e-07
 Identities = 29/86 (33%), Positives = 46/86 (53%), Gaps = 3/86 (3%)
 Frame = +1

Query: 7   FLDNMGFLEVETPLM-NXVPGGATAKPFITHHNELNMDLYMRI--APELYHKMLVVGGLD 177
           F ++  F EVETPL+    P GA  + F+   + LN   +  +  +P+ Y ++L+  G+ 
Sbjct: 172 FFNDREFCEVETPLLFKSTPEGA--REFVVP-SRLNPGKFYALPQSPQQYKQILMASGIG 228

Query: 178 RVYEIGRQFRNEGIDLTHNPEFTTCD 255
             Y+I R FR+E +     PEFT  D
Sbjct: 229 NYYQIARCFRDEDLRFDRQPEFTQID 254


>SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 580

 Score = 44.8 bits (101), Expect = 6e-06
 Identities = 28/86 (32%), Positives = 42/86 (48%), Gaps = 1/86 (1%)
 Frame = +1

Query: 1   RRFLDNMGFLEVETPLMNXVPGGATAKPFITHHNELNMDLYMRIAPELYHKMLVVGGLDR 180
           R FL +  F E+ TP M+       +  F   +     D ++  +P+LY +ML+    +R
Sbjct: 265 REFLLSNSFNEIHTPKMSGASSEGGSNVFKIQY--FKTDGFLSQSPQLYKQMLIAADRER 322

Query: 181 VYEIGRQFRNEGID-LTHNPEFTTCD 255
           V+EIG  FR E  +   H  EFT  D
Sbjct: 323 VFEIGPVFRAEDSNTYRHMTEFTGLD 348


>SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 499

 Score = 27.9 bits (59), Expect = 0.72
 Identities = 15/51 (29%), Positives = 25/51 (49%)
 Frame = +1

Query: 91  THHNELNMDLYMRIAPELYHKMLVVGGLDRVYEIGRQFRNEGIDLTHNPEF 243
           TH   ++ ++  ++A   +H         + + I R FRNE +D TH  EF
Sbjct: 327 THTTAVSANMLYKLAQNGFHPA-------KYFSIDRVFRNETVDATHLAEF 370


>SPCC4F11.04c |||mannosyltransferase complex subunit
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 345

 Score = 27.1 bits (57), Expect = 1.3
 Identities = 13/33 (39%), Positives = 16/33 (48%)
 Frame = +3

Query: 330 IHGSYKVKYHPEGPXGKEIESISXHPLLEYR*W 428
           IH ++K    PEG  G +   I  HP  EY  W
Sbjct: 86  IHQTWKTTEVPEGWKGAQQSCIDLHPDYEYILW 118


>SPBC1604.15 |gpi16||pig-T |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 545

 Score = 27.1 bits (57), Expect = 1.3
 Identities = 13/33 (39%), Positives = 20/33 (60%)
 Frame = -2

Query: 222 VDTFISELSSNFIYPIESTHNKHFVIKLRSYSH 124
           +DTF  + ++N   PI+ST + H V   RS S+
Sbjct: 315 IDTFSLDEAANIQIPIQSTSDNHTVTVDRSLSN 347


>SPCPJ732.02c |||xylulose kinase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 555

 Score = 26.2 bits (55), Expect = 2.2
 Identities = 16/37 (43%), Positives = 20/37 (54%)
 Frame = +1

Query: 109 NMDLYMRIAPELYHKMLVVGGLDRVYEIGRQFRNEGI 219
           N+D+ MRI P L      +   DRVY +G   RNE I
Sbjct: 430 NLDIRMRITPLLTG----IPQPDRVYVVGGASRNEAI 462


>SPCC1620.05 |||Rab geranylgeranyltransferase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 344

 Score = 25.4 bits (53), Expect = 3.9
 Identities = 11/30 (36%), Positives = 18/30 (60%)
 Frame = +3

Query: 267 YADYNDLMTITESILSGMVQSIHGSYKVKY 356
           Y ++N  M ITE +LS   ++ HG +  +Y
Sbjct: 123 YPNWNYEMMITEKLLSADARNFHGWHYRRY 152


>SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 713

 Score = 25.0 bits (52), Expect = 5.1
 Identities = 11/49 (22%), Positives = 22/49 (44%)
 Frame = +1

Query: 139 ELYHKMLVVGGLDRVYEIGRQFRNEGIDLTHNPEFTTCDFTWHMRIIMT 285
           ++YH      G D  YE+  +   + ++LT +P     +  W   + +T
Sbjct: 283 DIYHAFCKEVGKDLPYEVVGRRTGDVLNLTASPNRANSELKWKAELSIT 331


>SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 425

 Score = 24.6 bits (51), Expect = 6.7
 Identities = 9/12 (75%), Positives = 12/12 (100%)
 Frame = +1

Query: 178 RVYEIGRQFRNE 213
           RVY+IGR+FR+E
Sbjct: 136 RVYQIGRKFRDE 147


>SPBC16H5.12c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 682

 Score = 24.2 bits (50), Expect = 8.9
 Identities = 10/32 (31%), Positives = 16/32 (50%)
 Frame = +3

Query: 312 SGMVQSIHGSYKVKYHPEGPXGKEIESISXHP 407
           S +++ +  S    Y+P  P G    SI+ HP
Sbjct: 50  SPLIKKLPRSKNFDYYPSNPIGDLKNSIASHP 81


>SPAC222.11 |hem13||coproporphyrinogen III oxidase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 312

 Score = 24.2 bits (50), Expect = 8.9
 Identities = 12/37 (32%), Positives = 20/37 (54%)
 Frame = +3

Query: 300 ESILSGMVQSIHGSYKVKYHPEGPXGKEIESISXHPL 410
           ESIL  M   +H S++ KY P+    K +  ++  P+
Sbjct: 275 ESIL--MTLPLHASWRYKYEPKQERHKALLKVTHTPI 309


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,937,943
Number of Sequences: 5004
Number of extensions: 37268
Number of successful extensions: 110
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 106
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 108
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 158122380
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -