BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesS0402 (439 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 155 3e-39 SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|... 109 2e-25 SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch... 48 6e-07 SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe... 45 6e-06 SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 28 0.72 SPCC4F11.04c |||mannosyltransferase complex subunit |Schizosacch... 27 1.3 SPBC1604.15 |gpi16||pig-T |Schizosaccharomyces pombe|chr 2|||Manual 27 1.3 SPCPJ732.02c |||xylulose kinase |Schizosaccharomyces pombe|chr 3... 26 2.2 SPCC1620.05 |||Rab geranylgeranyltransferase |Schizosaccharomyce... 25 3.9 SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces pomb... 25 5.1 SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|ch... 25 6.7 SPBC16H5.12c |||conserved fungal protein|Schizosaccharomyces pom... 24 8.9 SPAC222.11 |hem13||coproporphyrinogen III oxidase |Schizosacchar... 24 8.9 >SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 2|||Manual Length = 591 Score = 155 bits (376), Expect = 3e-39 Identities = 68/86 (79%), Positives = 77/86 (89%) Frame = +1 Query: 1 RRFLDNMGFLEVETPLMNXVPGGATAKPFITHHNELNMDLYMRIAPELYHKMLVVGGLDR 180 R F D+ F+EVETP+MN + GGATAKPF+THHN+L+MDLYMRIAPELY KMLVVGGLDR Sbjct: 259 RHFFDSRDFMEVETPMMNMIAGGATAKPFVTHHNDLDMDLYMRIAPELYLKMLVVGGLDR 318 Query: 181 VYEIGRQFRNEGIDLTHNPEFTTCDF 258 VYEIGRQFRNEG DLTHNPEFT+ +F Sbjct: 319 VYEIGRQFRNEGADLTHNPEFTSIEF 344 Score = 67.3 bits (157), Expect = 1e-12 Identities = 29/45 (64%), Positives = 34/45 (75%) Frame = +3 Query: 255 FYMAYADYNDLMTITESILSGMVQSIHGSYKVKYHPEGPXGKEIE 389 FY AYADY DLM TE +LSG+V+ + GSYKV YHPEGP G + E Sbjct: 344 FYQAYADYYDLMDTTEELLSGLVKDLTGSYKVPYHPEGPEGPKWE 388 >SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|||Manual Length = 531 Score = 109 bits (261), Expect = 2e-25 Identities = 49/85 (57%), Positives = 65/85 (76%) Frame = +1 Query: 1 RRFLDNMGFLEVETPLMNXVPGGATAKPFITHHNELNMDLYMRIAPELYHKMLVVGGLDR 180 R+F GFLEVETP+++ GGATA+PFIT + L +R APEL+ K LV+GG++R Sbjct: 215 RKFFSERGFLEVETPILSHHFGGATARPFITSDIH-KLPLTLRCAPELWLKQLVIGGMNR 273 Query: 181 VYEIGRQFRNEGIDLTHNPEFTTCD 255 V+E+G+ FRNEGID THNPEFT+C+ Sbjct: 274 VFELGKNFRNEGIDATHNPEFTSCE 298 >SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr 3|||Manual Length = 611 Score = 48.0 bits (109), Expect = 6e-07 Identities = 29/86 (33%), Positives = 46/86 (53%), Gaps = 3/86 (3%) Frame = +1 Query: 7 FLDNMGFLEVETPLM-NXVPGGATAKPFITHHNELNMDLYMRI--APELYHKMLVVGGLD 177 F ++ F EVETPL+ P GA + F+ + LN + + +P+ Y ++L+ G+ Sbjct: 172 FFNDREFCEVETPLLFKSTPEGA--REFVVP-SRLNPGKFYALPQSPQQYKQILMASGIG 228 Query: 178 RVYEIGRQFRNEGIDLTHNPEFTTCD 255 Y+I R FR+E + PEFT D Sbjct: 229 NYYQIARCFRDEDLRFDRQPEFTQID 254 >SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe|chr 3|||Manual Length = 580 Score = 44.8 bits (101), Expect = 6e-06 Identities = 28/86 (32%), Positives = 42/86 (48%), Gaps = 1/86 (1%) Frame = +1 Query: 1 RRFLDNMGFLEVETPLMNXVPGGATAKPFITHHNELNMDLYMRIAPELYHKMLVVGGLDR 180 R FL + F E+ TP M+ + F + D ++ +P+LY +ML+ +R Sbjct: 265 REFLLSNSFNEIHTPKMSGASSEGGSNVFKIQY--FKTDGFLSQSPQLYKQMLIAADRER 322 Query: 181 VYEIGRQFRNEGID-LTHNPEFTTCD 255 V+EIG FR E + H EFT D Sbjct: 323 VFEIGPVFRAEDSNTYRHMTEFTGLD 348 >SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 499 Score = 27.9 bits (59), Expect = 0.72 Identities = 15/51 (29%), Positives = 25/51 (49%) Frame = +1 Query: 91 THHNELNMDLYMRIAPELYHKMLVVGGLDRVYEIGRQFRNEGIDLTHNPEF 243 TH ++ ++ ++A +H + + I R FRNE +D TH EF Sbjct: 327 THTTAVSANMLYKLAQNGFHPA-------KYFSIDRVFRNETVDATHLAEF 370 >SPCC4F11.04c |||mannosyltransferase complex subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 345 Score = 27.1 bits (57), Expect = 1.3 Identities = 13/33 (39%), Positives = 16/33 (48%) Frame = +3 Query: 330 IHGSYKVKYHPEGPXGKEIESISXHPLLEYR*W 428 IH ++K PEG G + I HP EY W Sbjct: 86 IHQTWKTTEVPEGWKGAQQSCIDLHPDYEYILW 118 >SPBC1604.15 |gpi16||pig-T |Schizosaccharomyces pombe|chr 2|||Manual Length = 545 Score = 27.1 bits (57), Expect = 1.3 Identities = 13/33 (39%), Positives = 20/33 (60%) Frame = -2 Query: 222 VDTFISELSSNFIYPIESTHNKHFVIKLRSYSH 124 +DTF + ++N PI+ST + H V RS S+ Sbjct: 315 IDTFSLDEAANIQIPIQSTSDNHTVTVDRSLSN 347 >SPCPJ732.02c |||xylulose kinase |Schizosaccharomyces pombe|chr 3|||Manual Length = 555 Score = 26.2 bits (55), Expect = 2.2 Identities = 16/37 (43%), Positives = 20/37 (54%) Frame = +1 Query: 109 NMDLYMRIAPELYHKMLVVGGLDRVYEIGRQFRNEGI 219 N+D+ MRI P L + DRVY +G RNE I Sbjct: 430 NLDIRMRITPLLTG----IPQPDRVYVVGGASRNEAI 462 >SPCC1620.05 |||Rab geranylgeranyltransferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 344 Score = 25.4 bits (53), Expect = 3.9 Identities = 11/30 (36%), Positives = 18/30 (60%) Frame = +3 Query: 267 YADYNDLMTITESILSGMVQSIHGSYKVKY 356 Y ++N M ITE +LS ++ HG + +Y Sbjct: 123 YPNWNYEMMITEKLLSADARNFHGWHYRRY 152 >SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces pombe|chr 2|||Manual Length = 713 Score = 25.0 bits (52), Expect = 5.1 Identities = 11/49 (22%), Positives = 22/49 (44%) Frame = +1 Query: 139 ELYHKMLVVGGLDRVYEIGRQFRNEGIDLTHNPEFTTCDFTWHMRIIMT 285 ++YH G D YE+ + + ++LT +P + W + +T Sbjct: 283 DIYHAFCKEVGKDLPYEVVGRRTGDVLNLTASPNRANSELKWKAELSIT 331 >SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 425 Score = 24.6 bits (51), Expect = 6.7 Identities = 9/12 (75%), Positives = 12/12 (100%) Frame = +1 Query: 178 RVYEIGRQFRNE 213 RVY+IGR+FR+E Sbjct: 136 RVYQIGRKFRDE 147 >SPBC16H5.12c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 682 Score = 24.2 bits (50), Expect = 8.9 Identities = 10/32 (31%), Positives = 16/32 (50%) Frame = +3 Query: 312 SGMVQSIHGSYKVKYHPEGPXGKEIESISXHP 407 S +++ + S Y+P P G SI+ HP Sbjct: 50 SPLIKKLPRSKNFDYYPSNPIGDLKNSIASHP 81 >SPAC222.11 |hem13||coproporphyrinogen III oxidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 312 Score = 24.2 bits (50), Expect = 8.9 Identities = 12/37 (32%), Positives = 20/37 (54%) Frame = +3 Query: 300 ESILSGMVQSIHGSYKVKYHPEGPXGKEIESISXHPL 410 ESIL M +H S++ KY P+ K + ++ P+ Sbjct: 275 ESIL--MTLPLHASWRYKYEPKQERHKALLKVTHTPI 309 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,937,943 Number of Sequences: 5004 Number of extensions: 37268 Number of successful extensions: 110 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 106 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 108 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 158122380 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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