BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesS0402 (439 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative / lysin... 158 2e-39 At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) f... 114 3e-26 At4g33760.1 68417.m04793 tRNA synthetase class II (D, K and N) f... 57 5e-09 At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative / as... 42 2e-04 At4g31180.2 68417.m04427 aspartyl-tRNA synthetase, putative / as... 31 0.26 At4g31180.1 68417.m04426 aspartyl-tRNA synthetase, putative / as... 31 0.26 At1g02205.2 68414.m00154 CER1 protein identical to maize gl1 hom... 27 5.5 At1g02205.1 68414.m00153 CER1 protein identical to maize gl1 hom... 27 5.5 At5g19290.1 68418.m02299 esterase/lipase/thioesterase family pro... 27 7.3 At1g13320.1 68414.m01546 serine/threonine protein phosphatase 2A... 26 9.7 >At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative / lysine--tRNA ligase, putative similar to SP|Q43776 Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) {Lycopersicon esculentum}; contains Pfam profile PF00152: tRNA synthetases class II (D, K and N) Length = 626 Score = 158 bits (383), Expect = 2e-39 Identities = 68/86 (79%), Positives = 78/86 (90%) Frame = +1 Query: 1 RRFLDNMGFLEVETPLMNXVPGGATAKPFITHHNELNMDLYMRIAPELYHKMLVVGGLDR 180 RRFLDN FLEVETP+MN + GGA A+PF+THHN+L+M LYMRIAPELY K L+VGGL+R Sbjct: 291 RRFLDNKNFLEVETPMMNMIAGGAAARPFVTHHNDLDMRLYMRIAPELYLKQLIVGGLER 350 Query: 181 VYEIGRQFRNEGIDLTHNPEFTTCDF 258 VYEIG+QFRNEGIDLTHNPEFTTC+F Sbjct: 351 VYEIGKQFRNEGIDLTHNPEFTTCEF 376 Score = 58.8 bits (136), Expect = 1e-09 Identities = 26/45 (57%), Positives = 33/45 (73%) Frame = +3 Query: 255 FYMAYADYNDLMTITESILSGMVQSIHGSYKVKYHPEGPXGKEIE 389 FYMA+ADYNDLM +TE +LSGMV+ + G YK+KY+ G IE Sbjct: 376 FYMAFADYNDLMEMTEVMLSGMVKELTGGYKIKYNANGYDKDPIE 420 Score = 26.2 bits (55), Expect = 9.7 Identities = 10/17 (58%), Positives = 12/17 (70%) Frame = +2 Query: 389 IDFXPPFARVPMMATLE 439 IDF PPF R+ M+ LE Sbjct: 421 IDFTPPFRRIEMIGELE 437 >At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) family protein similar to SP|Q9RHV9 Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) {Bacillus stearothermophilus}; contains Pfam profile: PF00152 tRNA synthetases class II (D, K and N) Length = 602 Score = 114 bits (274), Expect = 3e-26 Identities = 49/85 (57%), Positives = 65/85 (76%) Frame = +1 Query: 1 RRFLDNMGFLEVETPLMNXVPGGATAKPFITHHNELNMDLYMRIAPELYHKMLVVGGLDR 180 R+ +++ G+LEVETP++ GGA A+PF+T HN L DLY+RIA EL+ K ++VGG ++ Sbjct: 263 RKTVESFGYLEVETPVLQGAAGGAEARPFVTFHNSLGRDLYLRIATELHLKRMLVGGFEK 322 Query: 181 VYEIGRQFRNEGIDLTHNPEFTTCD 255 VYEIGR FRNEGI HNPEFTT + Sbjct: 323 VYEIGRIFRNEGISTRHNPEFTTIE 347 Score = 28.3 bits (60), Expect = 2.4 Identities = 10/33 (30%), Positives = 20/33 (60%) Frame = +3 Query: 258 YMAYADYNDLMTITESILSGMVQSIHGSYKVKY 356 Y AY+DY+ +M + E I++ +++G + Y Sbjct: 349 YEAYSDYHSMMDMAELIVTQCSMAVNGKLTIDY 381 >At4g33760.1 68417.m04793 tRNA synthetase class II (D, K and N) family protein similar to SP|P36419 Aspartyl-tRNA synthetase (EC 6.1.1.12) (Aspartate--tRNA ligase) (AspRS) {Thermus thermophilus}; contains Pfam profile PF00152: tRNA synthetases class II (D, K and N) Length = 664 Score = 57.2 bits (132), Expect = 5e-09 Identities = 32/88 (36%), Positives = 52/88 (59%), Gaps = 3/88 (3%) Frame = +1 Query: 1 RRFL-DNMGFLEVETPLMNX-VPGGATAKPFITHHNELNMDLY-MRIAPELYHKMLVVGG 171 RR+L D GF+E+ETP+++ P GA + ++ + Y + +P+L+ +ML+V G Sbjct: 230 RRYLEDRHGFIEIETPILSRSTPEGA--RDYLVPSRIQSGTFYALPQSPQLFKQMLMVSG 287 Query: 172 LDRVYEIGRQFRNEGIDLTHNPEFTTCD 255 D+ Y+I R FR+E + PEFT D Sbjct: 288 FDKYYQIARCFRDEDLRADRQPEFTQLD 315 >At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative / aspartate--tRNA ligase, putative simialr to aspartate-tRNA ligase (EC 6.1.1.12) from Drosophila melanogaster GI:4512034, Homo sapiens SP|P14868, Rattus norvegicus SP|P15178; contains Pfam profile PF00152 tRNA synthetases class II (D, K and N) Length = 532 Score = 41.9 bits (94), Expect = 2e-04 Identities = 25/93 (26%), Positives = 44/93 (47%), Gaps = 1/93 (1%) Frame = +1 Query: 1 RRFLDNMGFLEVETPLMNXVPGGATAKPFITHHNELNMDLYMRIAPELYHKMLVVGGLDR 180 R +L + GFLE+ TP + + G + + + + +P+L+ +M + G + R Sbjct: 238 REYLQSKGFLEIHTPKL--IAGSSEGGSAVFRLDYKGQPACLAQSPQLHKQMAICGDMRR 295 Query: 181 VYEIGRQFRNE-GIDLTHNPEFTTCDFTWHMRI 276 V+E+G FR E H EF D +R+ Sbjct: 296 VFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRM 328 >At4g31180.2 68417.m04427 aspartyl-tRNA synthetase, putative / aspartate--tRNA ligase, putative similar to Aspartyl-tRNA synthetase (Aspartate--tRNA ligase) (AspRS) [Homo sapiens] GI:20178330 Length = 558 Score = 31.5 bits (68), Expect = 0.26 Identities = 17/48 (35%), Positives = 25/48 (52%), Gaps = 1/48 (2%) Frame = +1 Query: 133 APELYHKMLVVGGLDRVYEIGRQFRNE-GIDLTHNPEFTTCDFTWHMR 273 +P+L+ +M + G L RV+E+G FR E H EF D +R Sbjct: 306 SPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIR 353 >At4g31180.1 68417.m04426 aspartyl-tRNA synthetase, putative / aspartate--tRNA ligase, putative similar to Aspartyl-tRNA synthetase (Aspartate--tRNA ligase) (AspRS) [Homo sapiens] GI:20178330 Length = 558 Score = 31.5 bits (68), Expect = 0.26 Identities = 17/48 (35%), Positives = 25/48 (52%), Gaps = 1/48 (2%) Frame = +1 Query: 133 APELYHKMLVVGGLDRVYEIGRQFRNE-GIDLTHNPEFTTCDFTWHMR 273 +P+L+ +M + G L RV+E+G FR E H EF D +R Sbjct: 306 SPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIR 353 >At1g02205.2 68414.m00154 CER1 protein identical to maize gl1 homolog (glossy1 locus) GI:1209703 and CER1 GI:1199467 from [Arabidopsis thaliana] Length = 625 Score = 27.1 bits (57), Expect = 5.5 Identities = 17/63 (26%), Positives = 30/63 (47%), Gaps = 2/63 (3%) Frame = +1 Query: 94 HHNEL--NMDLYMRIAPELYHKMLVVGGLDRVYEIGRQFRNEGIDLTHNPEFTTCDFTWH 267 HH + N L+M + +Y M D +YE + ++ +D+ H TT + +H Sbjct: 251 HHTQFRTNYSLFMPLYDYIYGTM--DESTDTLYEKTLERGDDIVDVVHLTHLTTPESIYH 308 Query: 268 MRI 276 +RI Sbjct: 309 LRI 311 >At1g02205.1 68414.m00153 CER1 protein identical to maize gl1 homolog (glossy1 locus) GI:1209703 and CER1 GI:1199467 from [Arabidopsis thaliana] Length = 580 Score = 27.1 bits (57), Expect = 5.5 Identities = 17/63 (26%), Positives = 30/63 (47%), Gaps = 2/63 (3%) Frame = +1 Query: 94 HHNEL--NMDLYMRIAPELYHKMLVVGGLDRVYEIGRQFRNEGIDLTHNPEFTTCDFTWH 267 HH + N L+M + +Y M D +YE + ++ +D+ H TT + +H Sbjct: 251 HHTQFRTNYSLFMPLYDYIYGTM--DESTDTLYEKTLERGDDIVDVVHLTHLTTPESIYH 308 Query: 268 MRI 276 +RI Sbjct: 309 LRI 311 >At5g19290.1 68418.m02299 esterase/lipase/thioesterase family protein low similarity to monoglyceride lipase [Homo sapiens] GI:14594904; contains Interpro entry IPR000379 Length = 330 Score = 26.6 bits (56), Expect = 7.3 Identities = 16/49 (32%), Positives = 24/49 (48%), Gaps = 4/49 (8%) Frame = +1 Query: 25 FLEVETPLMNXVPGGATAKPFIT----HHNELNMDLYMRIAPELYHKML 159 F EVE PL+ GG H ++ D ++I PEL+H+M+ Sbjct: 247 FEEVEVPLLIVHGGGDVVCDVACVEELHRRAISEDKTIKIYPELWHQMI 295 >At1g13320.1 68414.m01546 serine/threonine protein phosphatase 2A (PP2A) 65 kDa regulatory subunit, putative similar to protein phosphatase 2A 65 kDa regulatory subunit GI:683502 from [Arabidopsis thaliana] Length = 587 Score = 26.2 bits (55), Expect = 9.7 Identities = 11/35 (31%), Positives = 23/35 (65%) Frame = +1 Query: 82 PFITHHNELNMDLYMRIAPELYHKMLVVGGLDRVY 186 PF++ +N+ + ++ + +A EL +L VGG++ Y Sbjct: 51 PFLSENNDDDDEVLLAMAEELGGFILYVGGVEYAY 85 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,108,982 Number of Sequences: 28952 Number of extensions: 194925 Number of successful extensions: 440 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 428 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 438 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 692941200 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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