SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= tesS0358
         (514 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M...   144   9e-36
SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|...   142   2e-35
SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz...   142   2e-35
SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi...   142   2e-35
SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch...   142   2e-35
SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro...    85   8e-18
SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pomb...    44   1e-05
SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc...    42   4e-05
SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosacc...    42   5e-05
SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomy...    27   1.6  
SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|...    27   2.2  
SPBC19F5.05c |ppp1|SPBC25D12.01c|pescadillo-family BRCT domain p...    26   3.8  
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces...    25   6.7  
SPAC19A8.12 |dcp2||mRNA decapping complex subunit Dcp2|Schizosac...    25   6.7  
SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate syntha...    25   6.7  
SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyce...    25   6.7  
SPBC2F12.09c |atf21||transcription factor Atf21|Schizosaccharomy...    25   8.8  

>SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 382

 Score =  144 bits (348), Expect = 9e-36
 Identities = 72/87 (82%), Positives = 78/87 (89%)
 Frame = +1

Query: 1   KKS*ILRVVYFSNTMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFA 180
           K+S +  V+     MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFA
Sbjct: 63  KESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFA 122

Query: 181 GKQLEDGRTLSDYNIQKESTLHLVLDL 261
           GKQLEDGRTLSDYNIQKESTLHLVL L
Sbjct: 123 GKQLEDGRTLSDYNIQKESTLHLVLRL 149



 Score =  144 bits (348), Expect = 9e-36
 Identities = 72/87 (82%), Positives = 78/87 (89%)
 Frame = +1

Query: 1   KKS*ILRVVYFSNTMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFA 180
           K+S +  V+     MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFA
Sbjct: 139 KESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFA 198

Query: 181 GKQLEDGRTLSDYNIQKESTLHLVLDL 261
           GKQLEDGRTLSDYNIQKESTLHLVL L
Sbjct: 199 GKQLEDGRTLSDYNIQKESTLHLVLRL 225



 Score =  144 bits (348), Expect = 9e-36
 Identities = 72/87 (82%), Positives = 78/87 (89%)
 Frame = +1

Query: 1   KKS*ILRVVYFSNTMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFA 180
           K+S +  V+     MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFA
Sbjct: 215 KESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFA 274

Query: 181 GKQLEDGRTLSDYNIQKESTLHLVLDL 261
           GKQLEDGRTLSDYNIQKESTLHLVL L
Sbjct: 275 GKQLEDGRTLSDYNIQKESTLHLVLRL 301



 Score =  144 bits (348), Expect = 9e-36
 Identities = 72/87 (82%), Positives = 78/87 (89%)
 Frame = +1

Query: 1   KKS*ILRVVYFSNTMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFA 180
           K+S +  V+     MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFA
Sbjct: 291 KESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFA 350

Query: 181 GKQLEDGRTLSDYNIQKESTLHLVLDL 261
           GKQLEDGRTLSDYNIQKESTLHLVL L
Sbjct: 351 GKQLEDGRTLSDYNIQKESTLHLVLRL 377



 Score =  142 bits (345), Expect = 2e-35
 Identities = 69/73 (94%), Positives = 72/73 (98%)
 Frame = +1

Query: 43  MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 222
           MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60

Query: 223 IQKESTLHLVLDL 261
           IQKESTLHLVL L
Sbjct: 61  IQKESTLHLVLRL 73


>SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein
           Ubi2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 128

 Score =  142 bits (345), Expect = 2e-35
 Identities = 69/73 (94%), Positives = 72/73 (98%)
 Frame = +1

Query: 43  MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 222
           MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60

Query: 223 IQKESTLHLVLDL 261
           IQKESTLHLVL L
Sbjct: 61  IQKESTLHLVLRL 73



 Score =  102 bits (244), Expect = 4e-23
 Identities = 44/52 (84%), Positives = 45/52 (86%)
 Frame = +3

Query: 255 RLRGGTIEPSLRILAMKYNCEKMICRKCYARLHPRATNCRKTKCGHTNNLRP 410
           RLRGG IEPSL+ LA KYNCEK ICRKCYARL PRATNCRK KCGHTN LRP
Sbjct: 72  RLRGGIIEPSLKALASKYNCEKQICRKCYARLPPRATNCRKKKCGHTNQLRP 123


>SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein
           Ubi1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 128

 Score =  142 bits (345), Expect = 2e-35
 Identities = 69/73 (94%), Positives = 72/73 (98%)
 Frame = +1

Query: 43  MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 222
           MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60

Query: 223 IQKESTLHLVLDL 261
           IQKESTLHLVL L
Sbjct: 61  IQKESTLHLVLRL 73



 Score =  102 bits (244), Expect = 4e-23
 Identities = 44/52 (84%), Positives = 45/52 (86%)
 Frame = +3

Query: 255 RLRGGTIEPSLRILAMKYNCEKMICRKCYARLHPRATNCRKTKCGHTNNLRP 410
           RLRGG IEPSL+ LA KYNCEK ICRKCYARL PRATNCRK KCGHTN LRP
Sbjct: 72  RLRGGIIEPSLKALASKYNCEKQICRKCYARLPPRATNCRKKKCGHTNQLRP 123


>SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein
           Ubi3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 150

 Score =  142 bits (345), Expect = 2e-35
 Identities = 69/73 (94%), Positives = 72/73 (98%)
 Frame = +1

Query: 43  MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 222
           MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60

Query: 223 IQKESTLHLVLDL 261
           IQKESTLHLVL L
Sbjct: 61  IQKESTLHLVLRL 73


>SPAC589.10c |||ribomal-ubiquitin fusion protein
           Ubi5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 150

 Score =  142 bits (345), Expect = 2e-35
 Identities = 69/73 (94%), Positives = 72/73 (98%)
 Frame = +1

Query: 43  MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 222
           MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60

Query: 223 IQKESTLHLVLDL 261
           IQKESTLHLVL L
Sbjct: 61  IQKESTLHLVLRL 73


>SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like
           protein modifier Ned8|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 78

 Score = 84.6 bits (200), Expect = 8e-18
 Identities = 37/73 (50%), Positives = 53/73 (72%)
 Frame = +1

Query: 43  MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 222
           M I VKTLTGK I L+++ +D +  +K ++++KEGIPP QQRLI+AGKQ+ D +    Y+
Sbjct: 1   MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYH 60

Query: 223 IQKESTLHLVLDL 261
           ++  S LHLVL L
Sbjct: 61  LEGGSVLHLVLAL 73


>SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 368

 Score = 44.4 bits (100), Expect = 1e-05
 Identities = 19/71 (26%), Positives = 41/71 (57%)
 Frame = +1

Query: 73  KTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 252
           K +  +V A   I  +K KIQ ++    ++Q+LI++G+ L D +T+ +YNI+++  +  +
Sbjct: 12  KFVISDVSADTKISELKEKIQTQQNYEVERQKLIYSGRILADDKTVGEYNIKEQDFIVCM 71

Query: 253 LDLEEVQLNLP 285
           +   +   + P
Sbjct: 72  VSRPKTSTSTP 82


>SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 354

 Score = 42.3 bits (95), Expect = 4e-05
 Identities = 21/73 (28%), Positives = 39/73 (53%)
 Frame = +1

Query: 43  MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 222
           + + +K    +   + V++  ++  +K  I     I  ++QRLI+AG+ L+D  +L  Y 
Sbjct: 4   ISLTIKAANDQKYAVTVDSESSVLALKEAIAPVADIEKERQRLIYAGRVLKDEESLKTYK 63

Query: 223 IQKESTLHLVLDL 261
           IQ   ++HLV  L
Sbjct: 64  IQDGHSIHLVKTL 76


>SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase
           Ubp6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 467

 Score = 41.9 bits (94), Expect = 5e-05
 Identities = 21/72 (29%), Positives = 39/72 (54%)
 Frame = +1

Query: 70  GKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHL 249
           GK   LE+E ++T   +K ++     +PP++Q++I  G QL+D   L    I+  +TL +
Sbjct: 10  GKKYDLEIEPNETGSTLKHQLYSLTQVPPERQKVIVKGGQLKDDVLLGSVGIKPNATLLM 69

Query: 250 VLDLEEVQLNLP 285
           +    E+   +P
Sbjct: 70  MGTAGELPTAMP 81


>SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 603

 Score = 27.1 bits (57), Expect = 1.6
 Identities = 24/87 (27%), Positives = 39/87 (44%), Gaps = 3/87 (3%)
 Frame = +1

Query: 46  QIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSD--Y 219
           +I V T+  K    +V  + T+  +K  I      P D+ +LI AG+ L +   L +  +
Sbjct: 5   RIRVTTVDQKVGIFQVPRTKTVLELKELIAVTFEAPADRLKLIHAGRVLRNETPLEEILH 64

Query: 220 NIQKESTLHLVLDL-EEVQLNLPFAFS 297
           +     T HLV+ +       LP A S
Sbjct: 65  DATDLVTFHLVIAVFNSTSTTLPSATS 91


>SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase
           Prp16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1173

 Score = 26.6 bits (56), Expect = 2.2
 Identities = 14/48 (29%), Positives = 28/48 (58%)
 Frame = +1

Query: 115 NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLD 258
           +++A  + KE    ++ R+I +G     G   SDY++ +E  +HL++D
Sbjct: 331 SIEASDRFKENSMWEKNRMITSGVSKAPGLE-SDYSLMEERRVHLLVD 377


>SPBC19F5.05c |ppp1|SPBC25D12.01c|pescadillo-family BRCT domain
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 607

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 13/29 (44%), Positives = 16/29 (55%)
 Frame = -3

Query: 317 LTVILHGENAKGRFNCTSSKSKTR*SVDS 231
           L  I+HG N   R N  + KSK R  V+S
Sbjct: 271 LPAIVHGNNKNARKNIATLKSKIRDIVNS 299


>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 3131

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 10/27 (37%), Positives = 16/27 (59%)
 Frame = -2

Query: 123  FDIFDSVGSFHLQCNGLPRKGFDEYLH 43
            F  ++S+G + L C    +  FDEYL+
Sbjct: 2887 FKPYESLGKYMLSCLDDGKYAFDEYLY 2913


>SPAC19A8.12 |dcp2||mRNA decapping complex subunit
           Dcp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 741

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 14/52 (26%), Positives = 24/52 (46%)
 Frame = +1

Query: 16  LRVVYFSNTMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRL 171
           L V  +S +  +  KTL        +EA++  + +K   Q K  +PP +  L
Sbjct: 618 LEVKNYSKSTDLLKKTLRIPRNDEPLEAANQFDLLKVSPQQKSEVPPKRNEL 669


>SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate
           synthase|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 541

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 11/24 (45%), Positives = 15/24 (62%)
 Frame = -2

Query: 117 IFDSVGSFHLQCNGLPRKGFDEYL 46
           IF  VG+F   C+ L ++GF E L
Sbjct: 43  IFPGVGNFGFVCDSLAKQGFLEPL 66


>SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 818

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 10/27 (37%), Positives = 16/27 (59%)
 Frame = -3

Query: 329 ANHFLTVILHGENAKGRFNCTSSKSKT 249
           A+H   +++HG +  G FN   S+S T
Sbjct: 437 ASHSNPIMMHGNDFGGNFNNMQSRSTT 463


>SPBC2F12.09c |atf21||transcription factor Atf21|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 355

 Score = 24.6 bits (51), Expect = 8.8
 Identities = 18/64 (28%), Positives = 25/64 (39%), Gaps = 3/64 (4%)
 Frame = +1

Query: 64  LTGKTITLEVEASDTIENVKAKIQDKEGIP---PDQQRLIFAGKQLEDGRTLSDYNIQKE 234
           L G   T EV    T+   +    + +G P   P  +  I A     +G TL+ YN    
Sbjct: 94  LKGPERTSEVSLPQTVNLSEISNNNDKGQPTNTPPVRSTIVAPSLYSEGSTLNHYNNGSH 153

Query: 235 STLH 246
             LH
Sbjct: 154 QVLH 157


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,110,710
Number of Sequences: 5004
Number of extensions: 42116
Number of successful extensions: 117
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 111
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 117
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 206265012
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -