Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesS0342
(548 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q99798 Cluster: Aconitate hydratase, mitochondrial prec... 91 2e-17
UniRef50_UPI00015B4325 Cluster: PREDICTED: similar to aconitase,... 90 4e-17
UniRef50_Q9VIE8 Cluster: CG9244-PB; n=37; cellular organisms|Rep... 88 1e-16
UniRef50_Q0CSK9 Cluster: Aconitate hydratase, mitochondrial; n=2... 87 2e-16
UniRef50_O13966 Cluster: Aconitate hydratase, mitochondrial prec... 83 3e-15
UniRef50_P19414 Cluster: Aconitate hydratase, mitochondrial prec... 82 7e-15
UniRef50_Q4AHJ7 Cluster: Aconitate hydratase; n=1; Chlorobium ph... 64 2e-09
UniRef50_Q2UTF0 Cluster: Aconitase/homoaconitase; n=9; cellular ... 62 8e-09
UniRef50_Q0USA6 Cluster: Putative uncharacterized protein; n=2; ... 60 3e-08
UniRef50_Q4PB22 Cluster: Putative uncharacterized protein; n=5; ... 58 1e-07
UniRef50_Q5AVN8 Cluster: Putative uncharacterized protein; n=1; ... 54 2e-06
UniRef50_A0RTP9 Cluster: 3-isopropylmalate isomerase/aconitase A... 47 3e-04
UniRef50_Q74AD1 Cluster: Aconitate hydratase, putative; n=12; Ba... 33 4.4
>UniRef50_Q99798 Cluster: Aconitate hydratase, mitochondrial
precursor; n=28; cellular organisms|Rep: Aconitate
hydratase, mitochondrial precursor - Homo sapiens
(Human)
Length = 780
Score = 91.1 bits (216), Expect = 2e-17
Identities = 45/54 (83%), Positives = 46/54 (85%)
Frame = +2
Query: 257 LTLSEKILYSHLDXPKGQEIERGASYLRLXPDRVAXQDATAQMAMLQFISSGPS 418
LTLSEKI+Y HLD P QEIERG SYLRL PDRVA QDATAQMAMLQFISSG S
Sbjct: 63 LTLSEKIVYGHLDDPASQEIERGKSYLRLRPDRVAMQDATAQMAMLQFISSGLS 116
Score = 50.4 bits (115), Expect = 3e-05
Identities = 34/115 (29%), Positives = 52/115 (45%), Gaps = 1/115 (0%)
Frame = +3
Query: 126 RCFSVSPLXAAAAQVAMSKFDKVP-LXYEKLTKNLEVVKKRLGRN*PCLRKSYILTWMXP 302
R + V+ + A+VAMS F+ + Y+ L KN+ +V+KRL R K P
Sbjct: 18 RQYHVASVLCQRAKVAMSHFEPNEYIHYDLLEKNINIVRKRLNRPLTLSEKIVYGHLDDP 77
Query: 303 KXRKLNAAQVISACXPTVWPXKTPLHKWXCYNLSLPGLPRXAVPSTIHCDHLIRS 467
+++ + P + + GL + AVPSTIHCDHLI +
Sbjct: 78 ASQEIERGKSYLRLRPDRVAMQDATAQMAMLQFISSGLSKVAVPSTIHCDHLIEA 132
>UniRef50_UPI00015B4325 Cluster: PREDICTED: similar to aconitase,
mitochondrial; n=1; Nasonia vitripennis|Rep: PREDICTED:
similar to aconitase, mitochondrial - Nasonia
vitripennis
Length = 917
Score = 89.8 bits (213), Expect = 4e-17
Identities = 44/52 (84%), Positives = 46/52 (88%)
Frame = +2
Query: 257 LTLSEKILYSHLDXPKGQEIERGASYLRLXPDRVAXQDATAQMAMLQFISSG 412
LTLSEKILYSHLD P+ Q+I RG SYLRL PDRVA QDATAQMAMLQFISSG
Sbjct: 197 LTLSEKILYSHLDEPQKQDIVRGTSYLRLRPDRVAMQDATAQMAMLQFISSG 248
Score = 83.0 bits (196), Expect = 4e-15
Identities = 49/118 (41%), Positives = 62/118 (52%), Gaps = 1/118 (0%)
Frame = +3
Query: 117 IQQRCFSVSPLXAAAAQVAMSKFDKVP-LXYEKLTKNLEVVKKRLGRN*PCLRKSYILTW 293
+QQRCFS SPL AAA+VAMSKFD L Y+KL +N+++VKKRL R K
Sbjct: 149 VQQRCFSTSPLTFAAAKVAMSKFDSTAYLPYDKLDENIKIVKKRLDRPLTLSEKILYSHL 208
Query: 294 MXPKXRKLNAAQVISACXPTVWPXKTPLHKWXCYNLSLPGLPRXAVPSTIHCDHLIRS 467
P+ + + P + + GLPR AVPSTIHCDHLI +
Sbjct: 209 DEPQKQDIVRGTSYLRLRPDRVAMQDATAQMAMLQFISSGLPRVAVPSTIHCDHLIEA 266
>UniRef50_Q9VIE8 Cluster: CG9244-PB; n=37; cellular organisms|Rep:
CG9244-PB - Drosophila melanogaster (Fruit fly)
Length = 787
Score = 88.2 bits (209), Expect = 1e-16
Identities = 42/52 (80%), Positives = 45/52 (86%)
Frame = +2
Query: 257 LTLSEKILYSHLDXPKGQEIERGASYLRLXPDRVAXQDATAQMAMLQFISSG 412
LTLSEK+LYSHLD P Q+I RG SYLRL PDRVA QDATAQMA+LQFISSG
Sbjct: 70 LTLSEKVLYSHLDDPANQDIVRGTSYLRLRPDRVAMQDATAQMALLQFISSG 121
Score = 54.4 bits (125), Expect = 2e-06
Identities = 37/104 (35%), Positives = 48/104 (46%), Gaps = 1/104 (0%)
Frame = +3
Query: 159 AAQVAMSKFDK-VPLXYEKLTKNLEVVKKRLGRN*PCLRKSYILTWMXPKXRKLNAAQVI 335
A++VA+SKFD V L YEKL K LEVV+ RL R K P + +
Sbjct: 36 ASKVALSKFDSDVYLPYEKLNKRLEVVRGRLNRPLTLSEKVLYSHLDDPANQDIVRGTSY 95
Query: 336 SACXPTVWPXKTPLHKWXCYNLSLPGLPRXAVPSTIHCDHLIRS 467
P + + GL + AVPST+HCDHLI +
Sbjct: 96 LRLRPDRVAMQDATAQMALLQFISSGLKKVAVPSTVHCDHLIEA 139
>UniRef50_Q0CSK9 Cluster: Aconitate hydratase, mitochondrial; n=2;
Pezizomycotina|Rep: Aconitate hydratase, mitochondrial -
Aspergillus terreus (strain NIH 2624)
Length = 781
Score = 87.4 bits (207), Expect = 2e-16
Identities = 43/63 (68%), Positives = 50/63 (79%), Gaps = 1/63 (1%)
Frame = +2
Query: 257 LTLSEKILYSHLDXPKGQEIERGASYLRLXPDRVAXQDATAQMAMLQFISSG-PSPXXCT 433
LT +EK+LYSHLD P GQ+IERG SYL+L PDRVA QDATAQMA+LQF+S+G PS T
Sbjct: 93 LTYAEKVLYSHLDDPHGQDIERGVSYLKLRPDRVACQDATAQMAILQFMSAGMPSVATPT 152
Query: 434 VHH 442
H
Sbjct: 153 TVH 155
Score = 37.5 bits (83), Expect = 0.20
Identities = 21/86 (24%), Positives = 36/86 (41%)
Frame = +3
Query: 210 KLTKNLEVVKKRLGRN*PCLRKSYILTWMXPKXRKLNAAQVISACXPTVWPXKTPLHKWX 389
K+++NL++V++RL R K P + + P + +
Sbjct: 77 KMSENLDIVRRRLSRPLTYAEKVLYSHLDDPHGQDIERGVSYLKLRPDRVACQDATAQMA 136
Query: 390 CYNLSLPGLPRXAVPSTIHCDHLIRS 467
G+P A P+T+HCDHLI +
Sbjct: 137 ILQFMSAGMPSVATPTTVHCDHLIEA 162
>UniRef50_O13966 Cluster: Aconitate hydratase, mitochondrial
precursor; n=21; cellular organisms|Rep: Aconitate
hydratase, mitochondrial precursor - Schizosaccharomyces
pombe (Fission yeast)
Length = 778
Score = 83.4 bits (197), Expect = 3e-15
Identities = 39/52 (75%), Positives = 44/52 (84%)
Frame = +2
Query: 257 LTLSEKILYSHLDXPKGQEIERGASYLRLXPDRVAXQDATAQMAMLQFISSG 412
LT SEKILY HLD P Q+IERG SYL+L PDRVA QDATAQMA+LQF+S+G
Sbjct: 59 LTYSEKILYGHLDDPVNQDIERGVSYLKLRPDRVACQDATAQMAILQFMSAG 110
Score = 53.2 bits (122), Expect = 4e-06
Identities = 37/117 (31%), Positives = 53/117 (45%), Gaps = 3/117 (2%)
Frame = +3
Query: 126 RCFSVSPLXAA--AAQVAMSKFDKVP-LXYEKLTKNLEVVKKRLGRN*PCLRKSYILTWM 296
R FS +P+ A A +VAMS F+K + Y+++ NLE+VKKRL R K
Sbjct: 12 RSFSCAPVAANIDAKKVAMSNFEKNKFINYQRIKDNLEIVKKRLNRPLTYSEKILYGHLD 71
Query: 297 XPKXRKLNAAQVISACXPTVWPXKTPLHKWXCYNLSLPGLPRXAVPSTIHCDHLIRS 467
P + + P + + G+P AVP T+HCDHLI +
Sbjct: 72 DPVNQDIERGVSYLKLRPDRVACQDATAQMAILQFMSAGMPEVAVPVTVHCDHLIEA 128
>UniRef50_P19414 Cluster: Aconitate hydratase, mitochondrial
precursor; n=41; cellular organisms|Rep: Aconitate
hydratase, mitochondrial precursor - Saccharomyces
cerevisiae (Baker's yeast)
Length = 778
Score = 82.2 bits (194), Expect = 7e-15
Identities = 37/51 (72%), Positives = 44/51 (86%)
Frame = +2
Query: 260 TLSEKILYSHLDXPKGQEIERGASYLRLXPDRVAXQDATAQMAMLQFISSG 412
T +EKILY HLD P GQ+I+RG SYL+L PDRVA QDATAQMA+LQF+S+G
Sbjct: 60 TYAEKILYGHLDDPHGQDIQRGVSYLKLRPDRVACQDATAQMAILQFMSAG 110
Score = 35.1 bits (77), Expect = 1.1
Identities = 22/90 (24%), Positives = 37/90 (41%)
Frame = +3
Query: 198 LXYEKLTKNLEVVKKRLGRN*PCLRKSYILTWMXPKXRKLNAAQVISACXPTVWPXKTPL 377
+ Y++ + L++V+KRL R K P + + P +
Sbjct: 39 INYKQNVETLDIVRKRLNRPFTYAEKILYGHLDDPHGQDIQRGVSYLKLRPDRVACQDAT 98
Query: 378 HKWXCYNLSLPGLPRXAVPSTIHCDHLIRS 467
+ GLP+ A P T+HCDHLI++
Sbjct: 99 AQMAILQFMSAGLPQVAKPVTVHCDHLIQA 128
>UniRef50_Q4AHJ7 Cluster: Aconitate hydratase; n=1; Chlorobium
phaeobacteroides BS1|Rep: Aconitate hydratase -
Chlorobium phaeobacteroides BS1
Length = 141
Score = 64.1 bits (149), Expect = 2e-09
Identities = 31/53 (58%), Positives = 39/53 (73%), Gaps = 1/53 (1%)
Frame = +2
Query: 257 LTLSEKILYSHLDXP-KGQEIERGASYLRLXPDRVAXQDATAQMAMLQFISSG 412
+TL+EKILY+HLD +RG SY+ PDRVA QDATAQMA+LQF+ +G
Sbjct: 43 MTLAEKILYAHLDGELPSASFDRGNSYVDFRPDRVAMQDATAQMALLQFMQAG 95
Score = 32.3 bits (70), Expect = 7.6
Identities = 12/21 (57%), Positives = 17/21 (80%)
Frame = +3
Query: 411 GLPRXAVPSTIHCDHLIRSPS 473
G P+ AV S++HCDHLI++ S
Sbjct: 95 GKPQAAVSSSVHCDHLIQAKS 115
>UniRef50_Q2UTF0 Cluster: Aconitase/homoaconitase; n=9; cellular
organisms|Rep: Aconitase/homoaconitase - Aspergillus
oryzae
Length = 806
Score = 62.1 bits (144), Expect = 8e-09
Identities = 34/57 (59%), Positives = 40/57 (70%), Gaps = 5/57 (8%)
Frame = +2
Query: 257 LTLSEKILYSHLDXPKG-----QEIERGASYLRLXPDRVAXQDATAQMAMLQFISSG 412
LTL+EK+LYSHL QEI+RG + L L PDRVA DATA MA+LQFIS+G
Sbjct: 67 LTLTEKLLYSHLIPSDDKVWSLQEIDRGKTILELRPDRVACHDATATMALLQFISAG 123
Score = 33.5 bits (73), Expect = 3.3
Identities = 14/19 (73%), Positives = 16/19 (84%)
Frame = +3
Query: 411 GLPRXAVPSTIHCDHLIRS 467
GLPR AVP+T+H DHLI S
Sbjct: 123 GLPRVAVPTTVHGDHLIVS 141
>UniRef50_Q0USA6 Cluster: Putative uncharacterized protein; n=2;
Pezizomycotina|Rep: Putative uncharacterized protein -
Phaeosphaeria nodorum (Septoria nodorum)
Length = 781
Score = 60.5 bits (140), Expect = 3e-08
Identities = 34/61 (55%), Positives = 45/61 (73%), Gaps = 9/61 (14%)
Frame = +2
Query: 257 LTLSEKILYSHLDXPK---------GQEIERGASYLRLXPDRVAXQDATAQMAMLQFISS 409
LTL+EKILY+HL+ P+ G++I RG + L+L PDRVA QDA+AQMA+LQF+S
Sbjct: 71 LTLAEKILYAHLENPEESLLSNTNNGRDI-RGQANLKLKPDRVAMQDASAQMALLQFMSC 129
Query: 410 G 412
G
Sbjct: 130 G 130
>UniRef50_Q4PB22 Cluster: Putative uncharacterized protein; n=5;
Fungi/Metazoa group|Rep: Putative uncharacterized
protein - Ustilago maydis (Smut fungus)
Length = 1041
Score = 58.4 bits (135), Expect = 1e-07
Identities = 33/60 (55%), Positives = 42/60 (70%), Gaps = 8/60 (13%)
Frame = +2
Query: 257 LTLSEKILYSHLDXPK------GQEIE--RGASYLRLXPDRVAXQDATAQMAMLQFISSG 412
LTLSEKILYSHL P+ G ++ RG YL+L DR+A QDA+AQMA+LQF++ G
Sbjct: 171 LTLSEKILYSHLRNPEHDLAGVGADVSAIRGKKYLKLKIDRLAMQDASAQMALLQFMTCG 230
Score = 37.9 bits (84), Expect = 0.15
Identities = 13/19 (68%), Positives = 18/19 (94%)
Frame = +3
Query: 411 GLPRXAVPSTIHCDHLIRS 467
GLPR A+PS++HCDHLI++
Sbjct: 230 GLPRTAIPSSVHCDHLIQA 248
>UniRef50_Q5AVN8 Cluster: Putative uncharacterized protein; n=1;
Emericella nidulans|Rep: Putative uncharacterized
protein - Emericella nidulans (Aspergillus nidulans)
Length = 326
Score = 54.0 bits (124), Expect = 2e-06
Identities = 26/47 (55%), Positives = 33/47 (70%)
Frame = +2
Query: 257 LTLSEKILYSHLDXPKGQEIERGASYLRLXPDRVAXQDATAQMAMLQ 397
LT +EK+LY+HLD I RG + LR P R+A QDATAQMA++Q
Sbjct: 55 LTYAEKVLYNHLDDEFDGNIVRGQTQLRSKPVRIACQDATAQMALIQ 101
>UniRef50_A0RTP9 Cluster: 3-isopropylmalate isomerase/aconitase A;
n=7; cellular organisms|Rep: 3-isopropylmalate
isomerase/aconitase A - Cenarchaeum symbiosum
Length = 754
Score = 47.2 bits (107), Expect = 3e-04
Identities = 26/50 (52%), Positives = 31/50 (62%), Gaps = 1/50 (2%)
Frame = +2
Query: 257 LTLSEKILYSHLDXPKGQEIE-RGASYLRLXPDRVAXQDATAQMAMLQFI 403
LTLSEKI+ H+ G +I Y+ L PDRVA QD T QM MLQF+
Sbjct: 32 LTLSEKIMAGHM-VRDGTDIPVENKDYVHLTPDRVALQDVTGQMVMLQFM 80
>UniRef50_Q74AD1 Cluster: Aconitate hydratase, putative; n=12;
Bacteria|Rep: Aconitate hydratase, putative - Geobacter
sulfurreducens
Length = 645
Score = 33.1 bits (72), Expect = 4.4
Identities = 23/50 (46%), Positives = 29/50 (58%)
Frame = +2
Query: 263 LSEKILYSHLDXPKGQEIERGASYLRLXPDRVAXQDATAQMAMLQFISSG 412
L+ KIL +HL KG E+ G + L D QDAT MAML+FI+ G
Sbjct: 5 LATKILEAHL--VKG-ELTPGTE-IALKIDHALLQDATGTMAMLEFIAMG 50
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 431,307,526
Number of Sequences: 1657284
Number of extensions: 6771427
Number of successful extensions: 13151
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 12918
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 13143
length of database: 575,637,011
effective HSP length: 96
effective length of database: 416,537,747
effective search space used: 35822246242
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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