SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= tesS0328
         (508 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo...   110   1e-25
SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo...   110   1e-25
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    28   0.92 
SPAC12G12.07c |||conserved fungal protein|Schizosaccharomyces po...    27   1.6  
SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c...    26   2.8  
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces...    26   2.8  
SPAC23H4.02 |ppk9||serine/threonine protein kinase Ppk9 |Schizos...    26   3.7  
SPAC3C7.07c |||arginine-tRNA protein transferase |Schizosaccharo...    25   6.5  
SPBC530.01 |gyp1||GTPase activating protein Gyp1 |Schizosaccharo...    25   8.6  

>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
           L2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score =  110 bits (265), Expect = 1e-25
 Identities = 52/85 (61%), Positives = 54/85 (63%)
 Frame = +2

Query: 254 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 433
           QTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH        
Sbjct: 61  QTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQNEK 120

Query: 434 XXXXXXXXXXXXXXXXXQARGHIIE 508
                             ARGH IE
Sbjct: 121 RYAIASAVAASGVPSLLLARGHRIE 145



 Score = 67.7 bits (158), Expect = 9e-13
 Identities = 31/58 (53%), Positives = 40/58 (68%)
 Frame = +1

Query: 73  LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVRRR 246
           ++ ARP VS+Y+ K  +V      LPFVFKAPIRPDLV  VH +++KN RQPY V  +
Sbjct: 1   MAAARPTVSIYN-KDGSVSSETLALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEK 57


>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
           L2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score =  110 bits (265), Expect = 1e-25
 Identities = 52/85 (61%), Positives = 54/85 (63%)
 Frame = +2

Query: 254 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 433
           QTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH        
Sbjct: 61  QTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQNEK 120

Query: 434 XXXXXXXXXXXXXXXXXQARGHIIE 508
                             ARGH IE
Sbjct: 121 RYAISSAVAASGVPSLLLARGHRIE 145



 Score = 68.5 bits (160), Expect = 5e-13
 Identities = 32/58 (55%), Positives = 40/58 (68%)
 Frame = +1

Query: 73  LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVRRR 246
           ++ ARP VS+YS K  +V      LPFVFKAPIRPDLV  VH +++KN RQPY V  +
Sbjct: 1   MAAARPTVSIYS-KDGSVSSETIALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEK 57


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 27.9 bits (59), Expect = 0.92
 Identities = 23/71 (32%), Positives = 32/71 (45%)
 Frame = -2

Query: 369  PPRHMLPKAP*PDLWVPPPRTRGIRATARPVPHDSALVCDQPPSHAVGLPRVLGHRNVNI 190
            PP+   P  P P + VPPP +    A   P    SA     P S A  +P   G R+  +
Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPS----APPMPAGPPSAPPPPLPASSAPSVPNP-GDRSA-L 1752

Query: 189  IDQVRTYGRLE 157
            + Q+ T  RL+
Sbjct: 1753 LQQIHTGTRLK 1763


>SPAC12G12.07c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 412

 Score = 27.1 bits (57), Expect = 1.6
 Identities = 18/47 (38%), Positives = 22/47 (46%)
 Frame = +2

Query: 215 TRGSPTA*EGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGN 355
           TRGS        SQ S +    GR   R  R RGG  +RS +G + N
Sbjct: 345 TRGSSKRPSVNRSQGSGQRGRGGRGFYRGGRGRGGFFNRSKRGQYSN 391


>SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 606

 Score = 26.2 bits (55), Expect = 2.8
 Identities = 10/26 (38%), Positives = 17/26 (65%)
 Frame = -3

Query: 134 APCTVSLFSEYTDTKGRATDRLISLL 57
           A C V ++SEYT   G ++  L++L+
Sbjct: 490 ADCVVKIWSEYTKNTGESSPVLVALV 515


>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 713

 Score = 26.2 bits (55), Expect = 2.8
 Identities = 24/76 (31%), Positives = 30/76 (39%), Gaps = 2/76 (2%)
 Frame = -2

Query: 384 GANIRPPRHMLPKAP*PDLWVPPPRTR--GIRATARPVPHDSALVCDQPPSHAVGLPRVL 211
           GA   PP    P    P L     R+R   I  +  P P    L+   P S  +G    L
Sbjct: 336 GAFSAPPAPFFPLGFSPHLNDESSRSRWSNISYSPPPPPPPPELLNHSPKSRPLGDKPYL 395

Query: 210 GHRNVNIIDQVRTYGR 163
            +RN  I  + RT GR
Sbjct: 396 FYRNNQIGPRTRTEGR 411


>SPAC23H4.02 |ppk9||serine/threonine protein kinase Ppk9
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 532

 Score = 25.8 bits (54), Expect = 3.7
 Identities = 13/37 (35%), Positives = 19/37 (51%)
 Frame = +3

Query: 72  SIGSPTFSVGVFREE*DGAGCSQAPPVRIQGAHTSGP 182
           SI SP+ SVG   +  D +  S + P+ I G  +  P
Sbjct: 312 SITSPSSSVGQIPQPTDHSALSPSKPMSISGTESPNP 348


>SPAC3C7.07c |||arginine-tRNA protein transferase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 361

 Score = 25.0 bits (52), Expect = 6.5
 Identities = 9/22 (40%), Positives = 15/22 (68%), Gaps = 2/22 (9%)
 Frame = -3

Query: 311 GHAEFGQQHVR--YPMIRHWFV 252
           G++ F  +HV   +P ++HWFV
Sbjct: 320 GNSTFPTRHVLSYFPQVKHWFV 341


>SPBC530.01 |gyp1||GTPase activating protein Gyp1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 514

 Score = 24.6 bits (51), Expect = 8.6
 Identities = 11/32 (34%), Positives = 16/32 (50%)
 Frame = -2

Query: 312 RTRGIRATARPVPHDSALVCDQPPSHAVGLPR 217
           R R   + A+P   D ++  DQPP   + L R
Sbjct: 41  RGRSTSSKAQPFRLDDSVYSDQPPKDHISLAR 72


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,944,510
Number of Sequences: 5004
Number of extensions: 37247
Number of successful extensions: 122
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 113
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 120
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 202220600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -