BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesS0318 (549 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 173 1e-44 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 90 2e-19 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 87 1e-18 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 83 3e-17 SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 1.8 SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ... 25 5.6 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 173 bits (421), Expect = 1e-44 Identities = 80/134 (59%), Positives = 91/134 (67%) Frame = +3 Query: 66 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 245 MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60 Query: 246 PRASSSTWSPAPWTLYALDLXXXXXXXXXXXXXXXGAGNNWAKGHYTEGAELXDSVLDIV 425 PRA P GAGN WAKGHYTEGAEL D+VLD+V Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120 Query: 426 RKEAESCDCLQGFQ 467 R+EAE+CD LQGFQ Sbjct: 121 RREAEACDALQGFQ 134 Score = 30.3 bits (65), Expect = 0.20 Identities = 13/26 (50%), Positives = 16/26 (61%) Frame = +1 Query: 472 HTRSAAAXVPVSDLLISKIREEYPDR 549 H+ + LL+SKIREEYPDR Sbjct: 137 HSLGGGTGSGMGTLLLSKIREEYPDR 162 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 89.8 bits (213), Expect = 2e-19 Identities = 45/134 (33%), Positives = 71/134 (52%), Gaps = 2/134 (1%) Frame = +3 Query: 69 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 248 REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62 Query: 249 RASSSTWSPAPWTLYALDL--XXXXXXXXXXXXXXXGAGNNWAKGHYTEGAELXDSVLDI 422 RA P D GAGNNWA G Y+ + + ++D+ Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121 Query: 423 VRKEAESCDCLQGF 464 + +EA+ D L+GF Sbjct: 122 IDREADGSDSLEGF 135 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 87.4 bits (207), Expect = 1e-18 Identities = 49/135 (36%), Positives = 65/135 (48%), Gaps = 2/135 (1%) Frame = +3 Query: 66 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 239 MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 240 YVPRASSSTWSPAPWTLYALDLXXXXXXXXXXXXXXXGAGNNWAKGHYTEGAELXDSVLD 419 YVPR+ P A NN+A+GHYT G EL D V D Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120 Query: 420 IVRKEAESCDCLQGF 464 +R+ A++C LQGF Sbjct: 121 KIRRIADNCSGLQGF 135 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 83.0 bits (196), Expect = 3e-17 Identities = 46/139 (33%), Positives = 66/139 (47%), Gaps = 6/139 (4%) Frame = +3 Query: 66 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 227 MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60 Query: 228 SGGKYVPRASSSTWSPAPWTLYALDLXXXXXXXXXXXXXXXGAGNNWAKGHYTEGAELXD 407 GK+VPR+ P A NN+A+GHYT G E+ D Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120 Query: 408 SVLDIVRKEAESCDCLQGF 464 SVL+ +R+ A++C LQGF Sbjct: 121 SVLERIRRMADNCSGLQGF 139 >SPBC19G7.16 |iws1||transcription elongation factor complex subunit Iws1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 27.1 bits (57), Expect = 1.8 Identities = 10/34 (29%), Positives = 22/34 (64%) Frame = +3 Query: 105 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 206 N++G E+++++ +DPT A + DLQ++ + Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166 >SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1877 Score = 25.4 bits (53), Expect = 5.6 Identities = 18/71 (25%), Positives = 31/71 (43%), Gaps = 4/71 (5%) Frame = +1 Query: 181 TLTCSWSASMYTTMKPPAASTCPAPPRRLGA---RHHGLCT-LWTFRTDLPSGQLRLXNS 348 ++ CS S+Y + P A+ CP + L A +H L + + F + R+ N+ Sbjct: 95 SVLCSLDESVYPNLLPLTANICPCILKALLALVRQHRELSSDIVKFLNSIFLALTRILNT 154 Query: 349 PAPATTGPRDT 381 P + P T Sbjct: 155 PHSSNNNPTHT 165 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,188,724 Number of Sequences: 5004 Number of extensions: 43295 Number of successful extensions: 126 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 114 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 116 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 227943826 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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