SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= tesS0318
         (549 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   173   1e-44
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    90   2e-19
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...    87   1e-18
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...    83   3e-17
SPBC19G7.16 |iws1||transcription elongation factor complex subun...    27   1.8  
SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ...    25   5.6  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  173 bits (421), Expect = 1e-44
 Identities = 80/134 (59%), Positives = 91/134 (67%)
 Frame = +3

Query: 66  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 245
           MREIVHIQAGQCGNQ+GA FW  I+DEHG+D  G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60

Query: 246 PRASSSTWSPAPWTLYALDLXXXXXXXXXXXXXXXGAGNNWAKGHYTEGAELXDSVLDIV 425
           PRA      P                         GAGN WAKGHYTEGAEL D+VLD+V
Sbjct: 61  PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120

Query: 426 RKEAESCDCLQGFQ 467
           R+EAE+CD LQGFQ
Sbjct: 121 RREAEACDALQGFQ 134



 Score = 30.3 bits (65), Expect = 0.20
 Identities = 13/26 (50%), Positives = 16/26 (61%)
 Frame = +1

Query: 472 HTRSAAAXVPVSDLLISKIREEYPDR 549
           H+        +  LL+SKIREEYPDR
Sbjct: 137 HSLGGGTGSGMGTLLLSKIREEYPDR 162


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 89.8 bits (213), Expect = 2e-19
 Identities = 45/134 (33%), Positives = 71/134 (52%), Gaps = 2/134 (1%)
 Frame = +3

Query: 69  REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 248
           REI+ +QAGQCGNQIG++FW+ +  EHGI P G     +   ++R +V++ ++   +Y+P
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62

Query: 249 RASSSTWSPAPWTLYALDL--XXXXXXXXXXXXXXXGAGNNWAKGHYTEGAELXDSVLDI 422
           RA      P        D                  GAGNNWA G Y+    + + ++D+
Sbjct: 63  RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121

Query: 423 VRKEAESCDCLQGF 464
           + +EA+  D L+GF
Sbjct: 122 IDREADGSDSLEGF 135


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score = 87.4 bits (207), Expect = 1e-18
 Identities = 49/135 (36%), Positives = 65/135 (48%), Gaps = 2/135 (1%)
 Frame = +3

Query: 66  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 239
           MREI+ I  GQ G QIG   WE+   EHGI P G  + ++  Q      + +++E   GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 240 YVPRASSSTWSPAPWTLYALDLXXXXXXXXXXXXXXXGAGNNWAKGHYTEGAELXDSVLD 419
           YVPR+      P                          A NN+A+GHYT G EL D V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 420 IVRKEAESCDCLQGF 464
            +R+ A++C  LQGF
Sbjct: 121 KIRRIADNCSGLQGF 135


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score = 83.0 bits (196), Expect = 3e-17
 Identities = 46/139 (33%), Positives = 66/139 (47%), Gaps = 6/139 (4%)
 Frame = +3

Query: 66  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 227
           MRE++ +  GQ G QIG   WE+   EHGI P G        H ++    +    +++E 
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60

Query: 228 SGGKYVPRASSSTWSPAPWTLYALDLXXXXXXXXXXXXXXXGAGNNWAKGHYTEGAELXD 407
             GK+VPR+      P                          A NN+A+GHYT G E+ D
Sbjct: 61  GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120

Query: 408 SVLDIVRKEAESCDCLQGF 464
           SVL+ +R+ A++C  LQGF
Sbjct: 121 SVLERIRRMADNCSGLQGF 139


>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
           Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 428

 Score = 27.1 bits (57), Expect = 1.8
 Identities = 10/34 (29%), Positives = 22/34 (64%)
 Frame = +3

Query: 105 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 206
           N++G    E+++++  +DPT A   + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166


>SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1877

 Score = 25.4 bits (53), Expect = 5.6
 Identities = 18/71 (25%), Positives = 31/71 (43%), Gaps = 4/71 (5%)
 Frame = +1

Query: 181 TLTCSWSASMYTTMKPPAASTCPAPPRRLGA---RHHGLCT-LWTFRTDLPSGQLRLXNS 348
           ++ CS   S+Y  + P  A+ CP   + L A   +H  L + +  F   +     R+ N+
Sbjct: 95  SVLCSLDESVYPNLLPLTANICPCILKALLALVRQHRELSSDIVKFLNSIFLALTRILNT 154

Query: 349 PAPATTGPRDT 381
           P  +   P  T
Sbjct: 155 PHSSNNNPTHT 165


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,188,724
Number of Sequences: 5004
Number of extensions: 43295
Number of successful extensions: 126
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 114
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 116
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 227943826
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -