Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesS0300
(547 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g20360.1 68415.m02377 expressed protein 83 2e-16
At1g19540.1 68414.m02434 isoflavone reductase, putative similar ... 32 0.29
At1g75290.1 68414.m08746 isoflavone reductase, putative similar ... 30 0.88
At1g75280.1 68414.m08745 isoflavone reductase, putative identica... 30 0.88
At1g75300.1 68414.m08747 isoflavone reductase, putative identica... 29 1.5
At5g19060.1 68418.m02266 expressed protein ; expression supporte... 28 3.5
At3g46810.1 68416.m05081 DC1 domain-containing protein contains ... 28 4.7
At5g50310.1 68418.m06229 kelch repeat-containing protein similar... 27 8.2
At5g13490.1 68418.m01556 ADP, ATP carrier protein 2, mitochondri... 27 8.2
At3g04740.1 68416.m00510 expressed protein (SWP1) 27 8.2
>At2g20360.1 68415.m02377 expressed protein
Length = 402
Score = 82.6 bits (195), Expect = 2e-16
Identities = 39/100 (39%), Positives = 63/100 (63%)
Frame = +1
Query: 247 FRFTGFVGRYVCNKLGKIGTQLILPYRGDFYDAQRLKVCGDLGQVLFTPYHLLDEESIAK 426
F TGF+GRY+ +L K+G+Q+++P+RG + LK+ GDLGQV+ + DE+SI
Sbjct: 74 FGATGFLGRYLVQQLAKMGSQVLVPFRGSEDSPRHLKLMGDLGQVVPMKFDPRDEDSIKA 133
Query: 427 AVRYSNVVINLVGRDYEN*EFQIQ*CSLDGVRRIARICRE 546
+ +NVVINL+GR+YE F + + ++A + +E
Sbjct: 134 VMAKANVVINLIGREYETRNFSFEDANHHIAEKLALVAKE 173
Score = 38.7 bits (86), Expect = 0.003
Identities = 22/47 (46%), Positives = 34/47 (72%)
Frame = +2
Query: 116 HLNGSMSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIVATVFGS 256
HLNG+ + Y +++ ++ +LA ++GTGGRSS +GIVATVFG+
Sbjct: 34 HLNGTDNCRY--SSSLATKGVGHLA--RKGTGGRSSVSGIVATVFGA 76
>At1g19540.1 68414.m02434 isoflavone reductase, putative similar to
SP|P52577; contains isoflavone reductase domain PF02716
Length = 310
Score = 31.9 bits (69), Expect = 0.29
Identities = 24/75 (32%), Positives = 37/75 (49%), Gaps = 4/75 (5%)
Frame = +1
Query: 256 TGFVGRYVCNKLGKIGTQLILPYR----GDFYDAQRLKVCGDLGQVLFTPYHLLDEESIA 423
TG +G+ + + K G R D AQ ++ DLG + L D+ES+
Sbjct: 11 TGLIGKVLVEESAKSGHATFALVREASLSDPVKAQLVERFKDLGVTILYG-SLSDKESLV 69
Query: 424 KAVRYSNVVINLVGR 468
KA++ +VVI+ VGR
Sbjct: 70 KAIKQVDVVISAVGR 84
>At1g75290.1 68414.m08746 isoflavone reductase, putative similar to
SP|P52577 Isoflavone reductase homolog P3 (EC 1.3.1.-)
{Arabidopsis thaliana}; contains Pfam profile PF02716:
Isoflavone reductase
Length = 323
Score = 30.3 bits (65), Expect = 0.88
Identities = 22/73 (30%), Positives = 34/73 (46%), Gaps = 3/73 (4%)
Frame = +1
Query: 256 TGFVGRYVCNKLGKIG-TQLILPYRGDFYDAQRLKVCGDLGQVLFTPYH--LLDEESIAK 426
TG +G+ + K G + L L D + K + T H L D ES+ K
Sbjct: 14 TGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLHGDLNDHESLVK 73
Query: 427 AVRYSNVVINLVG 465
A++ ++VVI+ VG
Sbjct: 74 AIKQADVVISTVG 86
>At1g75280.1 68414.m08745 isoflavone reductase, putative identical
to SP|P52577 Isoflavone reductase homolog P3 (EC
1.3.1.-) {Arabidopsis thaliana}; contains Pfam profile
PF02716: isoflavone reductase
Length = 310
Score = 30.3 bits (65), Expect = 0.88
Identities = 21/73 (28%), Positives = 34/73 (46%), Gaps = 3/73 (4%)
Frame = +1
Query: 256 TGFVGRYVCNKLGKIG-TQLILPYRGDFYDAQRLKVCGDLGQVLFTPYH--LLDEESIAK 426
TG++G+++ K G + L D + K + T H L D ES+ K
Sbjct: 14 TGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGDLNDHESLVK 73
Query: 427 AVRYSNVVINLVG 465
A++ +VVI+ VG
Sbjct: 74 AIKQVDVVISTVG 86
>At1g75300.1 68414.m08747 isoflavone reductase, putative identical
to SP|P52577 Isoflavone reductase homolog P3 (EC
1.3.1.-) {Arabidopsis thaliana}; contains Pfam profile
PF02716: Isoflavone reductase
Length = 322
Score = 29.5 bits (63), Expect = 1.5
Identities = 21/74 (28%), Positives = 35/74 (47%), Gaps = 4/74 (5%)
Frame = +1
Query: 256 TGFVGRYVCNKLGKIGTQLILPYR----GDFYDAQRLKVCGDLGQVLFTPYHLLDEESIA 423
TG++G ++ K G R D ++ ++ DLG V L D ES+
Sbjct: 14 TGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLG-VTILHGDLNDHESLV 72
Query: 424 KAVRYSNVVINLVG 465
KA++ +VVI+ +G
Sbjct: 73 KAIKQVDVVISTIG 86
>At5g19060.1 68418.m02266 expressed protein ; expression supported
by MPSS
Length = 551
Score = 28.3 bits (60), Expect = 3.5
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = -3
Query: 236 RCR*SYCGHQYLAYMLQGWVYDPNCS*RL 150
RCR +C A GWVY +CS +L
Sbjct: 272 RCREPWCDGALSALESNGWVYSSHCSFKL 300
>At3g46810.1 68416.m05081 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 686
Score = 27.9 bits (59), Expect = 4.7
Identities = 16/66 (24%), Positives = 28/66 (42%)
Frame = +1
Query: 259 GFVGRYVCNKLGKIGTQLILPYRGDFYDAQRLKVCGDLGQVLFTPYHLLDEESIAKAVRY 438
GF G Y C+K + R D +D L+ G + P+ ++DE +I
Sbjct: 328 GFYGAYSCSKCSSYVVHSLCATRKDVWDMVELE--GTPEEEEIAPFEVVDENTIKHMSHD 385
Query: 439 SNVVIN 456
+++ N
Sbjct: 386 HDLIFN 391
>At5g50310.1 68418.m06229 kelch repeat-containing protein similar to
Kelch repeats protein 3 (SP:Q08979) [Saccharomyces
cerevisiae]; contains Pfam PF01344: Kelch motif (6
repeats)
Length = 666
Score = 27.1 bits (57), Expect = 8.2
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = +1
Query: 304 TQLILPYRGDFYDAQRLKVCGDL 372
T+LIL Y G+FY+ Q+ V GDL
Sbjct: 82 TELIL-YGGEFYNGQKTYVYGDL 103
>At5g13490.1 68418.m01556 ADP, ATP carrier protein 2, mitochondrial
/ ADP/ATP translocase 2 / adenine nucleotide
translocator 2 (ANT2) identical to SWISS-PROT:P40941
ADP,ATP carrier protein 2, mitochondrial precursor
(Adenine nucleotide translocator 2) [Arabidopsis
thaliana]
Length = 385
Score = 27.1 bits (57), Expect = 8.2
Identities = 11/38 (28%), Positives = 22/38 (57%)
Frame = +2
Query: 125 GSMSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIV 238
G+ S++++ + +Y+ R N + + GG FNG+V
Sbjct: 200 GASSLLFVYSLDYARTRLANDSKSAKKGGGERQFNGLV 237
>At3g04740.1 68416.m00510 expressed protein (SWP1)
Length = 1703
Score = 27.1 bits (57), Expect = 8.2
Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 2/50 (4%)
Frame = +2
Query: 164 SSDRKPNLA--AYKRGTGGRSSFNGIVATVFGSPDLSDAMCATNWEKLVP 307
S R+P L A +G + SF+ +V VFG ++ A+ + + LVP
Sbjct: 614 SGHRQPGLVDEALTEMSGSQLSFSSVVDGVFGLQKVTSALMSIDGHGLVP 663
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,504,641
Number of Sequences: 28952
Number of extensions: 229785
Number of successful extensions: 467
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 439
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 467
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1023490624
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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