BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesS0254 (548 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC2G2.04c |mmf1|pmf1|YjgF family protein Mmf1|Schizosaccharomy... 76 4e-15 SPAC1039.10 |mmf2|hpm1, SPAC922.01|homologous Pmf1p factor 1|Sch... 67 1e-12 SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c... 35 0.007 SPBC800.08 |gcd10||translation initiation factor eIF-3 gamma sub... 29 0.60 SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 1.8 SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosa... 26 3.2 SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos... 25 7.3 SPBC1105.01 |rrp12|SPBPB7E8.03|rRNA processing protein Rrp12|Sch... 25 7.3 SPBC19C2.02 |pmt1||DNA methyltransferase homolog|Schizosaccharom... 25 9.7 SPBPB21E7.04c ||SPAPB21E7.04c, SPAPB21E7.04c|S-adenosylmethionin... 25 9.7 SPBC4C3.03 |||homoserine kinase |Schizosaccharomyces pombe|chr 2... 25 9.7 SPCC777.14 |prp4||serine/threonine protein kinase Prp4|Schizosac... 25 9.7 >SPBC2G2.04c |mmf1|pmf1|YjgF family protein Mmf1|Schizosaccharomyces pombe|chr 2|||Manual Length = 162 Score = 75.8 bits (178), Expect = 4e-15 Identities = 43/103 (41%), Positives = 57/103 (55%) Frame = +2 Query: 239 SNKNNITSPEIYQPVGPYSQAILAXKTLYISGILGLDRDAQMVCGGAEAQTRQALDNLRH 418 S K I SP++ GPY+QAI A +Y SG + + + +++ G QTRQ L NL+ Sbjct: 37 STKTPINSPKL-SSAGPYNQAIKANGVIYCSGQIPV-ANGKVIEGTVGDQTRQCLLNLQE 94 Query: 419 VLEAGGASLESVVKTTVLLASMDDFQTFNKSMAEYFPKACPAR 547 VL G+SL +VK + LA MDDF NK E P PAR Sbjct: 95 VLTEAGSSLNKIVKVNIFLADMDDFAAVNKVYTEVLPDPKPAR 137 >SPAC1039.10 |mmf2|hpm1, SPAC922.01|homologous Pmf1p factor 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 126 Score = 67.3 bits (157), Expect = 1e-12 Identities = 33/88 (37%), Positives = 48/88 (54%) Frame = +2 Query: 284 GPYSQAILAXKTLYISGILGLDRDAQMVCGGAEAQTRQALDNLRHVLEAGGASLESVVKT 463 GPY+QA+ + ++ SG + +D V G + QTR ++NL VL G+SLE +VK Sbjct: 15 GPYNQAVKSGGLIFCSGQAAV-KDGNFVPGTIQEQTRLTIENLAEVLRVAGSSLEKLVKV 73 Query: 464 TVLLASMDDFQTFNKSMAEYFPKACPAR 547 + L +DDF N+ E P PAR Sbjct: 74 NIFLTDIDDFAAMNEVYKEMLPDPMPAR 101 >SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr 2|||Manual Length = 606 Score = 35.1 bits (77), Expect = 0.007 Identities = 17/49 (34%), Positives = 27/49 (55%) Frame = +2 Query: 380 EAQTRQALDNLRHVLEAGGASLESVVKTTVLLASMDDFQTFNKSMAEYF 526 + + A++NL +L G S ++V TV+L+SM F FN +YF Sbjct: 324 QGEAESAINNLNELLGTYGYSNKNVYFVTVILSSMSKFAEFNSVYNKYF 372 Score = 29.9 bits (64), Expect = 0.26 Identities = 12/22 (54%), Positives = 17/22 (77%) Frame = +2 Query: 281 VGPYSQAILAXKTLYISGILGL 346 +GPYSQ+I A ++ISG +GL Sbjct: 421 IGPYSQSICANGVVFISGQIGL 442 >SPBC800.08 |gcd10||translation initiation factor eIF-3 gamma subunit Gcd10|Schizosaccharomyces pombe|chr 2|||Manual Length = 462 Score = 28.7 bits (61), Expect = 0.60 Identities = 21/67 (31%), Positives = 32/67 (47%), Gaps = 2/67 (2%) Frame = +2 Query: 266 EIYQPVGPYSQAILAXKTLYISGILGLDRDAQMV--CGGAEAQTRQALDNLRHVLEAGGA 439 EIYQP + I KT + I + Q + C G + T++ +D LR ++AGG Sbjct: 57 EIYQP-----KKIRVLKTREVQYIEEEKKTNQELNDCRGNQLMTQEEIDELRANIKAGGL 111 Query: 440 SLESVVK 460 E +K Sbjct: 112 RAEEAIK 118 >SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a Pol2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 2199 Score = 27.1 bits (57), Expect = 1.8 Identities = 15/47 (31%), Positives = 25/47 (53%) Frame = -2 Query: 535 SFRKIFCHRFVESLEVVHASQQNSSFHDRLQRSATSFKYMSQIVQSL 395 +F +RFV SL + H + + +D+L S SF +S+I + L Sbjct: 1459 AFHFSISNRFVFSLFMPHLKKVEAIIYDKLPGSDMSFPSISKIYEEL 1505 >SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 533 Score = 26.2 bits (55), Expect = 3.2 Identities = 12/22 (54%), Positives = 14/22 (63%) Frame = +2 Query: 476 ASMDDFQTFNKSMAEYFPKACP 541 A MD F TF++SMA P A P Sbjct: 477 APMDQFSTFHQSMATLPPGAVP 498 >SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizosaccharomyces pombe|chr 1|||Manual Length = 950 Score = 25.0 bits (52), Expect = 7.3 Identities = 11/28 (39%), Positives = 17/28 (60%), Gaps = 2/28 (7%) Frame = +1 Query: 280 SRSVQSSNF--SGQDLIHFWNSRIGSRC 357 S+ +Q F SGQD++ FW +R+ C Sbjct: 504 SKDIQPLPFIESGQDILFFWIARMALLC 531 >SPBC1105.01 |rrp12|SPBPB7E8.03|rRNA processing protein Rrp12|Schizosaccharomyces pombe|chr 2|||Manual Length = 1001 Score = 25.0 bits (52), Expect = 7.3 Identities = 10/16 (62%), Positives = 12/16 (75%) Frame = -3 Query: 537 QALGKYSAIDLLKVWK 490 QAL KY A++ LK WK Sbjct: 703 QALPKYVALERLKTWK 718 >SPBC19C2.02 |pmt1||DNA methyltransferase homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 330 Score = 24.6 bits (51), Expect = 9.7 Identities = 10/29 (34%), Positives = 16/29 (55%) Frame = -3 Query: 384 ASAPPQTICASRSNPRIPEMYKVLXAKIA 298 A+ P +CA NP+ E+Y + K+A Sbjct: 26 ANIPADIVCAIDINPQANEIYNLNHGKLA 54 >SPBPB21E7.04c ||SPAPB21E7.04c, SPAPB21E7.04c|S-adenosylmethionine-dependent methyltransferase |Schizosaccharomyces pombe|chr 2|||Manual Length = 281 Score = 24.6 bits (51), Expect = 9.7 Identities = 10/28 (35%), Positives = 17/28 (60%) Frame = -1 Query: 191 VFGRQCRIF*AHFYSLDVNRSIVQMTKR 108 +FG+Q AH+YSL+VN ++ + Sbjct: 111 LFGKQLTDPSAHYYSLEVNPKFAKIASK 138 >SPBC4C3.03 |||homoserine kinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 338 Score = 24.6 bits (51), Expect = 9.7 Identities = 12/38 (31%), Positives = 20/38 (52%) Frame = -1 Query: 149 SLDVNRSIVQMTKR*HYRCSDSKSMEYLHKCYFIIIIP 36 SLDV ++++ T RC++ + Y K + I IP Sbjct: 56 SLDVQKNMITQTSLYVLRCNNISTFPYATKIHVINPIP 93 >SPCC777.14 |prp4||serine/threonine protein kinase Prp4|Schizosaccharomyces pombe|chr 3|||Manual Length = 477 Score = 24.6 bits (51), Expect = 9.7 Identities = 25/87 (28%), Positives = 40/87 (45%), Gaps = 12/87 (13%) Frame = +2 Query: 59 IYVGIPYSYYLNTDSVIFLSFELLTYLHL---KNKNELRRF---CTG------VRRQSFA 202 I +G PYS ++T SV +EL T L + N++ R+ C G ++R F Sbjct: 331 IILGFPYSCPIDTWSVGCSLYELYTGQILFPGRTNNQMLRYMMECKGKFSHKMLKRSQFL 390 Query: 203 QNEFSKKFE*Q*SNKNNITSPEIYQPV 283 + F F + + IT+ E +PV Sbjct: 391 NDHFDADFNFIQIDHDPITNQETRKPV 417 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,261,009 Number of Sequences: 5004 Number of extensions: 45754 Number of successful extensions: 140 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 134 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 138 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 227943826 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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