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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= tesS0254
         (548 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC2G2.04c |mmf1|pmf1|YjgF family protein Mmf1|Schizosaccharomy...    76   4e-15
SPAC1039.10 |mmf2|hpm1, SPAC922.01|homologous Pmf1p factor 1|Sch...    67   1e-12
SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c...    35   0.007
SPBC800.08 |gcd10||translation initiation factor eIF-3 gamma sub...    29   0.60 
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni...    27   1.8  
SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosa...    26   3.2  
SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos...    25   7.3  
SPBC1105.01 |rrp12|SPBPB7E8.03|rRNA processing protein Rrp12|Sch...    25   7.3  
SPBC19C2.02 |pmt1||DNA methyltransferase homolog|Schizosaccharom...    25   9.7  
SPBPB21E7.04c ||SPAPB21E7.04c, SPAPB21E7.04c|S-adenosylmethionin...    25   9.7  
SPBC4C3.03 |||homoserine kinase |Schizosaccharomyces pombe|chr 2...    25   9.7  
SPCC777.14 |prp4||serine/threonine protein kinase Prp4|Schizosac...    25   9.7  

>SPBC2G2.04c |mmf1|pmf1|YjgF family protein Mmf1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 162

 Score = 75.8 bits (178), Expect = 4e-15
 Identities = 43/103 (41%), Positives = 57/103 (55%)
 Frame = +2

Query: 239 SNKNNITSPEIYQPVGPYSQAILAXKTLYISGILGLDRDAQMVCGGAEAQTRQALDNLRH 418
           S K  I SP++    GPY+QAI A   +Y SG + +  + +++ G    QTRQ L NL+ 
Sbjct: 37  STKTPINSPKL-SSAGPYNQAIKANGVIYCSGQIPV-ANGKVIEGTVGDQTRQCLLNLQE 94

Query: 419 VLEAGGASLESVVKTTVLLASMDDFQTFNKSMAEYFPKACPAR 547
           VL   G+SL  +VK  + LA MDDF   NK   E  P   PAR
Sbjct: 95  VLTEAGSSLNKIVKVNIFLADMDDFAAVNKVYTEVLPDPKPAR 137


>SPAC1039.10 |mmf2|hpm1, SPAC922.01|homologous Pmf1p factor
           1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 126

 Score = 67.3 bits (157), Expect = 1e-12
 Identities = 33/88 (37%), Positives = 48/88 (54%)
 Frame = +2

Query: 284 GPYSQAILAXKTLYISGILGLDRDAQMVCGGAEAQTRQALDNLRHVLEAGGASLESVVKT 463
           GPY+QA+ +   ++ SG   + +D   V G  + QTR  ++NL  VL   G+SLE +VK 
Sbjct: 15  GPYNQAVKSGGLIFCSGQAAV-KDGNFVPGTIQEQTRLTIENLAEVLRVAGSSLEKLVKV 73

Query: 464 TVLLASMDDFQTFNKSMAEYFPKACPAR 547
            + L  +DDF   N+   E  P   PAR
Sbjct: 74  NIFLTDIDDFAAMNEVYKEMLPDPMPAR 101


>SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 606

 Score = 35.1 bits (77), Expect = 0.007
 Identities = 17/49 (34%), Positives = 27/49 (55%)
 Frame = +2

Query: 380 EAQTRQALDNLRHVLEAGGASLESVVKTTVLLASMDDFQTFNKSMAEYF 526
           + +   A++NL  +L   G S ++V   TV+L+SM  F  FN    +YF
Sbjct: 324 QGEAESAINNLNELLGTYGYSNKNVYFVTVILSSMSKFAEFNSVYNKYF 372



 Score = 29.9 bits (64), Expect = 0.26
 Identities = 12/22 (54%), Positives = 17/22 (77%)
 Frame = +2

Query: 281 VGPYSQAILAXKTLYISGILGL 346
           +GPYSQ+I A   ++ISG +GL
Sbjct: 421 IGPYSQSICANGVVFISGQIGL 442


>SPBC800.08 |gcd10||translation initiation factor eIF-3 gamma
           subunit Gcd10|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 462

 Score = 28.7 bits (61), Expect = 0.60
 Identities = 21/67 (31%), Positives = 32/67 (47%), Gaps = 2/67 (2%)
 Frame = +2

Query: 266 EIYQPVGPYSQAILAXKTLYISGILGLDRDAQMV--CGGAEAQTRQALDNLRHVLEAGGA 439
           EIYQP     + I   KT  +  I    +  Q +  C G +  T++ +D LR  ++AGG 
Sbjct: 57  EIYQP-----KKIRVLKTREVQYIEEEKKTNQELNDCRGNQLMTQEEIDELRANIKAGGL 111

Query: 440 SLESVVK 460
             E  +K
Sbjct: 112 RAEEAIK 118


>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
            Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2199

 Score = 27.1 bits (57), Expect = 1.8
 Identities = 15/47 (31%), Positives = 25/47 (53%)
 Frame = -2

Query: 535  SFRKIFCHRFVESLEVVHASQQNSSFHDRLQRSATSFKYMSQIVQSL 395
            +F     +RFV SL + H  +  +  +D+L  S  SF  +S+I + L
Sbjct: 1459 AFHFSISNRFVFSLFMPHLKKVEAIIYDKLPGSDMSFPSISKIYEEL 1505


>SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein
           Msa1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 533

 Score = 26.2 bits (55), Expect = 3.2
 Identities = 12/22 (54%), Positives = 14/22 (63%)
 Frame = +2

Query: 476 ASMDDFQTFNKSMAEYFPKACP 541
           A MD F TF++SMA   P A P
Sbjct: 477 APMDQFSTFHQSMATLPPGAVP 498


>SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase
           Vas1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 950

 Score = 25.0 bits (52), Expect = 7.3
 Identities = 11/28 (39%), Positives = 17/28 (60%), Gaps = 2/28 (7%)
 Frame = +1

Query: 280 SRSVQSSNF--SGQDLIHFWNSRIGSRC 357
           S+ +Q   F  SGQD++ FW +R+   C
Sbjct: 504 SKDIQPLPFIESGQDILFFWIARMALLC 531


>SPBC1105.01 |rrp12|SPBPB7E8.03|rRNA processing protein
           Rrp12|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1001

 Score = 25.0 bits (52), Expect = 7.3
 Identities = 10/16 (62%), Positives = 12/16 (75%)
 Frame = -3

Query: 537 QALGKYSAIDLLKVWK 490
           QAL KY A++ LK WK
Sbjct: 703 QALPKYVALERLKTWK 718


>SPBC19C2.02 |pmt1||DNA methyltransferase
           homolog|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 330

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 10/29 (34%), Positives = 16/29 (55%)
 Frame = -3

Query: 384 ASAPPQTICASRSNPRIPEMYKVLXAKIA 298
           A+ P   +CA   NP+  E+Y +   K+A
Sbjct: 26  ANIPADIVCAIDINPQANEIYNLNHGKLA 54


>SPBPB21E7.04c ||SPAPB21E7.04c,
           SPAPB21E7.04c|S-adenosylmethionine-dependent
           methyltransferase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 281

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 10/28 (35%), Positives = 17/28 (60%)
 Frame = -1

Query: 191 VFGRQCRIF*AHFYSLDVNRSIVQMTKR 108
           +FG+Q     AH+YSL+VN    ++  +
Sbjct: 111 LFGKQLTDPSAHYYSLEVNPKFAKIASK 138


>SPBC4C3.03 |||homoserine kinase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 338

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 12/38 (31%), Positives = 20/38 (52%)
 Frame = -1

Query: 149 SLDVNRSIVQMTKR*HYRCSDSKSMEYLHKCYFIIIIP 36
           SLDV ++++  T     RC++  +  Y  K + I  IP
Sbjct: 56  SLDVQKNMITQTSLYVLRCNNISTFPYATKIHVINPIP 93


>SPCC777.14 |prp4||serine/threonine protein kinase
           Prp4|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 477

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 25/87 (28%), Positives = 40/87 (45%), Gaps = 12/87 (13%)
 Frame = +2

Query: 59  IYVGIPYSYYLNTDSVIFLSFELLTYLHL---KNKNELRRF---CTG------VRRQSFA 202
           I +G PYS  ++T SV    +EL T   L   +  N++ R+   C G      ++R  F 
Sbjct: 331 IILGFPYSCPIDTWSVGCSLYELYTGQILFPGRTNNQMLRYMMECKGKFSHKMLKRSQFL 390

Query: 203 QNEFSKKFE*Q*SNKNNITSPEIYQPV 283
            + F   F     + + IT+ E  +PV
Sbjct: 391 NDHFDADFNFIQIDHDPITNQETRKPV 417


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,261,009
Number of Sequences: 5004
Number of extensions: 45754
Number of successful extensions: 140
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 227943826
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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