Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= tesS0205
         (598 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr...   157   9e-40
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|...   108   5e-25
SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch...    48   1e-06
SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe...    44   2e-05
SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |...    28   1.2  
SPAC56F8.07 |||dubious|Schizosaccharomyces pombe|chr 1|||Manual        26   3.6  
SPCPJ732.02c |||xylulose kinase |Schizosaccharomyces pombe|chr 3...    26   3.6  
SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch...    26   4.8  
SPCC1620.05 |||Rab geranylgeranyltransferase |Schizosaccharomyce...    25   6.3  
SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces pomb...    25   8.4  
SPBC1604.15 |gpi16||pig-T |Schizosaccharomyces pombe|chr 2|||Manual    25   8.4  

>SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 591

 Score =  157 bits (382), Expect = 9e-40
 Identities = 69/86 (80%), Positives = 78/86 (90%)
 Frame = +1

Query: 1   RRFLDNMGFLEVETPLMNMVPGGATAKPFITHHNELNMDLYMRIAPELYHKMLVVGGLDR 180
           R F D+  F+EVETP+MNM+ GGATAKPF+THHN+L+MDLYMRIAPELY KMLVVGGLDR
Sbjct: 259 RHFFDSRDFMEVETPMMNMIAGGATAKPFVTHHNDLDMDLYMRIAPELYLKMLVVGGLDR 318

Query: 181 VYEIGRQFRNEGIDLTHNPEFTTCDF 258
           VYEIGRQFRNEG DLTHNPEFT+ +F
Sbjct: 319 VYEIGRQFRNEGADLTHNPEFTSIEF 344



 Score =  110 bits (265), Expect = 1e-25
 Identities = 50/85 (58%), Positives = 62/85 (72%)
 Frame = +3

Query: 255 FYMAYADYNDLMTITESILSGMVQSIHGSYKVKYHPEGPEGKEIEIDFTPPFARVPMMAT 434
           FY AYADY DLM  TE +LSG+V+ + GSYKV YHPEGPEG + E+DF+ P+ R+ M+  
Sbjct: 344 FYQAYADYYDLMDTTEELLSGLVKDLTGSYKVPYHPEGPEGPKWELDFSRPWRRINMIEY 403

Query: 435 LERVLNVKLPAPDKLDTPEANDFLK 509
           LE  LN K P  D+L TPEAN FL+
Sbjct: 404 LEEKLNTKFPPGDQLHTPEANAFLR 428



 Score = 35.5 bits (78), Expect = 0.006
 Identities = 14/29 (48%), Positives = 20/29 (68%)
 Frame = +2

Query: 509 NLCNTHQIECSPXRTTARXLDKLVSVXLE 595
           +LC  H +EC+P +T +R LDKLV   +E
Sbjct: 429 DLCAKHGVECAPPQTCSRLLDKLVGEFIE 457


>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 531

 Score =  108 bits (260), Expect = 5e-25
 Identities = 49/85 (57%), Positives = 65/85 (76%)
 Frame = +1

Query: 1   RRFLDNMGFLEVETPLMNMVPGGATAKPFITHHNELNMDLYMRIAPELYHKMLVVGGLDR 180
           R+F    GFLEVETP+++   GGATA+PFIT      + L +R APEL+ K LV+GG++R
Sbjct: 215 RKFFSERGFLEVETPILSHHFGGATARPFITSDIH-KLPLTLRCAPELWLKQLVIGGMNR 273

Query: 181 VYEIGRQFRNEGIDLTHNPEFTTCD 255
           V+E+G+ FRNEGID THNPEFT+C+
Sbjct: 274 VFELGKNFRNEGIDATHNPEFTSCE 298


>SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 611

 Score = 48.0 bits (109), Expect = 1e-06
 Identities = 29/86 (33%), Positives = 46/86 (53%), Gaps = 3/86 (3%)
 Frame = +1

Query: 7   FLDNMGFLEVETPLM-NMVPGGATAKPFITHHNELNMDLYMRI--APELYHKMLVVGGLD 177
           F ++  F EVETPL+    P GA  + F+   + LN   +  +  +P+ Y ++L+  G+ 
Sbjct: 172 FFNDREFCEVETPLLFKSTPEGA--REFVVP-SRLNPGKFYALPQSPQQYKQILMASGIG 228

Query: 178 RVYEIGRQFRNEGIDLTHNPEFTTCD 255
             Y+I R FR+E +     PEFT  D
Sbjct: 229 NYYQIARCFRDEDLRFDRQPEFTQID 254


>SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 580

 Score = 44.0 bits (99), Expect = 2e-05
 Identities = 28/86 (32%), Positives = 42/86 (48%), Gaps = 1/86 (1%)
 Frame = +1

Query: 1   RRFLDNMGFLEVETPLMNMVPGGATAKPFITHHNELNMDLYMRIAPELYHKMLVVGGLDR 180
           R FL +  F E+ TP M+       +  F   +     D ++  +P+LY +ML+    +R
Sbjct: 265 REFLLSNSFNEIHTPKMSGASSEGGSNVFKIQY--FKTDGFLSQSPQLYKQMLIAADRER 322

Query: 181 VYEIGRQFRNEGID-LTHNPEFTTCD 255
           V+EIG  FR E  +   H  EFT  D
Sbjct: 323 VFEIGPVFRAEDSNTYRHMTEFTGLD 348


>SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 499

 Score = 27.9 bits (59), Expect = 1.2
 Identities = 15/51 (29%), Positives = 25/51 (49%)
 Frame = +1

Query: 91  THHNELNMDLYMRIAPELYHKMLVVGGLDRVYEIGRQFRNEGIDLTHNPEF 243
           TH   ++ ++  ++A   +H         + + I R FRNE +D TH  EF
Sbjct: 327 THTTAVSANMLYKLAQNGFHPA-------KYFSIDRVFRNETVDATHLAEF 370


>SPAC56F8.07 |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 183

 Score = 26.2 bits (55), Expect = 3.6
 Identities = 14/43 (32%), Positives = 19/43 (44%), Gaps = 2/43 (4%)
 Frame = -3

Query: 455 HVKNTFQCCHHR-YSSKGWCEIDFYFLTL-WSFWMIFDLVGSM 333
           H   T  CC    ++ K W  + FYF  L    WM  D+ G +
Sbjct: 121 HAFTTTWCCMFELFAEKKWMIMSFYFPYLAIPLWMAIDMGGRL 163


>SPCPJ732.02c |||xylulose kinase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 555

 Score = 26.2 bits (55), Expect = 3.6
 Identities = 16/37 (43%), Positives = 20/37 (54%)
 Frame = +1

Query: 109 NMDLYMRIAPELYHKMLVVGGLDRVYEIGRQFRNEGI 219
           N+D+ MRI P L      +   DRVY +G   RNE I
Sbjct: 430 NLDIRMRITPLLTG----IPQPDRVYVVGGASRNEAI 462


>SPBC16D10.05 |mok13||alpha-1,3-glucan synthase
            Mok13|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2358

 Score = 25.8 bits (54), Expect = 4.8
 Identities = 14/30 (46%), Positives = 19/30 (63%)
 Frame = +3

Query: 411  ARVPMMATLERVLNVKLPAPDKLDTPEAND 500
            AR P+   L++V   KLP PD LDT + +D
Sbjct: 1369 ARYPIFWGLKKV--GKLPNPDPLDTAQLDD 1396


>SPCC1620.05 |||Rab geranylgeranyltransferase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 344

 Score = 25.4 bits (53), Expect = 6.3
 Identities = 11/30 (36%), Positives = 18/30 (60%)
 Frame = +3

Query: 267 YADYNDLMTITESILSGMVQSIHGSYKVKY 356
           Y ++N  M ITE +LS   ++ HG +  +Y
Sbjct: 123 YPNWNYEMMITEKLLSADARNFHGWHYRRY 152


>SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 713

 Score = 25.0 bits (52), Expect = 8.4
 Identities = 11/49 (22%), Positives = 22/49 (44%)
 Frame = +1

Query: 139 ELYHKMLVVGGLDRVYEIGRQFRNEGIDLTHNPEFTTCDFTWHMRIIMT 285
           ++YH      G D  YE+  +   + ++LT +P     +  W   + +T
Sbjct: 283 DIYHAFCKEVGKDLPYEVVGRRTGDVLNLTASPNRANSELKWKAELSIT 331


>SPBC1604.15 |gpi16||pig-T |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 545

 Score = 25.0 bits (52), Expect = 8.4
 Identities = 12/33 (36%), Positives = 19/33 (57%)
 Frame = -2

Query: 222 VDTFISELSSNFIYPIEXTHNKHFVIKLRSYSH 124
           +DTF  + ++N   PI+ T + H V   RS S+
Sbjct: 315 IDTFSLDEAANIQIPIQSTSDNHTVTVDRSLSN 347


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,615,969
Number of Sequences: 5004
Number of extensions: 55211
Number of successful extensions: 143
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 260219058
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)