BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesS0174 (499 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 137 1e-33 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 92 4e-20 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 91 6e-20 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 62 5e-11 SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 28 0.68 SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Sc... 27 1.2 SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 s... 26 2.7 SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|ch... 26 3.6 SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|ch... 26 3.6 SPAC1834.02 |aro1||pentafunctional aromatic polypeptide Aro1 |Sc... 25 4.8 SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomy... 25 4.8 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 137 bits (331), Expect = 1e-33 Identities = 60/81 (74%), Positives = 71/81 (87%) Frame = +2 Query: 257 INVYYNEASGGKYVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSXAGNNXAKGHY 436 +NVY+NEA+GGKYVPRAV+VDLEPGTMD+V+SG FG +FRPDN ++GQS AGN AKGHY Sbjct: 47 LNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHY 106 Query: 437 TXGA*LVDSVLDVVRKEAEGC 499 T GA L D+VLDVVR+EAE C Sbjct: 107 TEGAELADAVLDVVRREAEAC 127 Score = 75.4 bits (177), Expect = 5e-15 Identities = 31/45 (68%), Positives = 36/45 (80%) Frame = +3 Query: 120 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLE 254 MREIVHIQAGQCGNQ+GA FW I+DEHG+D+ G Y G S+ Q E Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHE 45 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 92.3 bits (219), Expect = 4e-20 Identities = 37/80 (46%), Positives = 54/80 (67%) Frame = +2 Query: 260 NVYYNEASGGKYVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSXAGNNXAKGHYT 439 + +++E GKYVPR++ VDLEP +D VR+GP+ +F P+ + G+ A NN A+GHYT Sbjct: 50 STFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYT 109 Query: 440 XGA*LVDSVLDVVRKEAEGC 499 G LVD V D +R+ A+ C Sbjct: 110 VGKELVDEVTDKIRRIADNC 129 Score = 40.7 bits (91), Expect = 1e-04 Identities = 18/34 (52%), Positives = 20/34 (58%) Frame = +3 Query: 120 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATG 221 MREI+ I GQ G QIG WE+ EHGI G Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNG 34 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 91.5 bits (217), Expect = 6e-20 Identities = 36/78 (46%), Positives = 55/78 (70%) Frame = +2 Query: 266 YYNEASGGKYVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSXAGNNXAKGHYTXG 445 +++E GK+VPR++ VDLEP +D VR+GP+ +F P+ V G+ A NN A+GHYT G Sbjct: 56 FFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVG 115 Query: 446 A*LVDSVLDVVRKEAEGC 499 ++DSVL+ +R+ A+ C Sbjct: 116 KEMIDSVLERIRRMADNC 133 Score = 38.7 bits (86), Expect = 5e-04 Identities = 17/42 (40%), Positives = 25/42 (59%) Frame = +3 Query: 120 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDL 245 MRE++ + GQ G QIG WE+ EHGI G + +S++ Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEV 42 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 62.1 bits (144), Expect = 5e-11 Identities = 24/81 (29%), Positives = 54/81 (66%), Gaps = 2/81 (2%) Frame = +2 Query: 260 NVYYNEASGGKYVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--XAGNNXAKGH 433 +V++ ++ +Y+PRA+++DLEP ++++ S +G ++ P+N + ++ AGNN A G Sbjct: 49 DVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG- 107 Query: 434 YTXGA*LVDSVLDVVRKEAEG 496 Y+ + + ++D++ +EA+G Sbjct: 108 YSHAERIFEDIMDMIDREADG 128 Score = 50.4 bits (115), Expect = 1e-07 Identities = 20/33 (60%), Positives = 26/33 (78%) Frame = +3 Query: 123 REIVHIQAGQCGNQIGAKFWEVISDEHGIDATG 221 REI+ +QAGQCGNQIG++FW+ + EHGI G Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDG 35 >SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 28.3 bits (60), Expect = 0.68 Identities = 14/32 (43%), Positives = 17/32 (53%) Frame = -2 Query: 411 PAXDCPKTKLSGRKICPNGPERTESMVPGSRS 316 P+ PK L R I PNGPE + + GS S Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51 >SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1458 Score = 27.5 bits (58), Expect = 1.2 Identities = 17/58 (29%), Positives = 27/58 (46%) Frame = -3 Query: 284 LKLRYSRH*CFKLQVGVAAVSTRGVNAVLVRDYFPELCSDLIPALTRLDVDDFPHDCT 111 ++L SR C + G+ + V + +Y P S+ + + RLDVD F H T Sbjct: 599 VRLGISRKRCERYPFGILCIIFT-VLEIAAEEYSPNWESEELRLVNRLDVDSFLHPKT 655 >SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 subunit Alp6|Schizosaccharomyces pombe|chr 2|||Manual Length = 821 Score = 26.2 bits (55), Expect = 2.7 Identities = 10/35 (28%), Positives = 18/35 (51%) Frame = -3 Query: 188 YFPELCSDLIPALTRLDVDDFPHDCTKNLEPPESN 84 +F E+ + P +LD+DD + + P E+N Sbjct: 36 FFQEIIHSISPDTFQLDIDDILYKIYSKIPPEENN 70 >SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 25.8 bits (54), Expect = 3.6 Identities = 17/53 (32%), Positives = 26/53 (49%) Frame = +1 Query: 85 FDSGGSKFLVQS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPT 243 F S S S +STS RV++ ++ SSG + T+ + V +AT T Sbjct: 165 FVSPSSSSSSSSSAATSTSTRVSSSAKASTSSGAIAYTTKCVVVPVTASATAT 217 >SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 25.8 bits (54), Expect = 3.6 Identities = 17/53 (32%), Positives = 26/53 (49%) Frame = +1 Query: 85 FDSGGSKFLVQS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPT 243 F S S S +STS RV++ ++ SSG + T+ + V +AT T Sbjct: 165 FVSPSSSSSSSSSAATSTSTRVSSSAKASTSSGAIAYTTKCVVVPVTASATAT 217 >SPAC1834.02 |aro1||pentafunctional aromatic polypeptide Aro1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1573 Score = 25.4 bits (53), Expect = 4.8 Identities = 17/56 (30%), Positives = 25/56 (44%) Frame = +3 Query: 99 FQIFSAIMREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLEASMS 266 F + S I I C + +W+V+ G+ TGA S SD L+ S+S Sbjct: 807 FSVLSLICPSRTLIIDKACVEKTWPYWWDVLHQSFGVKLTGATSVASD-PLKGSIS 861 >SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 25.4 bits (53), Expect = 4.8 Identities = 7/21 (33%), Positives = 14/21 (66%) Frame = -1 Query: 145 AWMWTISLMIALKIWNPPNRI 83 AW+W ++LM+ + PN++ Sbjct: 458 AWLWFMALMLLFPSYQNPNKV 478 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,060,560 Number of Sequences: 5004 Number of extensions: 40809 Number of successful extensions: 123 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 116 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 123 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 196153982 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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