BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesS0135 (598 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g13570.1 68418.m01568 MutT/nudix family protein similar to mR... 58 6e-09 At5g02330.1 68418.m00156 DC1 domain-containing protein contains ... 29 2.4 At1g68760.1 68414.m07860 MutT/nudix family protein similar to SP... 29 3.1 At3g32260.1 68416.m04113 hypothetical protein 28 4.1 At1g30920.1 68414.m03782 F-box family protein contains F-box dom... 28 4.1 At4g12720.3 68417.m01998 MutT/nudix family protein similar to SP... 27 7.2 At4g12720.2 68417.m01997 MutT/nudix family protein similar to SP... 27 7.2 At4g12720.1 68417.m01996 MutT/nudix family protein similar to SP... 27 7.2 At3g07680.1 68416.m00921 emp24/gp25L/p24 family protein similar ... 27 7.2 At2g38450.1 68415.m04722 expressed protein 27 7.2 At1g10930.1 68414.m01255 DNA helicase (RECQl4A) nearly identical... 27 7.2 At1g03520.1 68414.m00333 glycosyltransferase family 14 protein /... 27 7.2 At5g48910.1 68418.m06051 pentatricopeptide (PPR) repeat-containi... 27 9.5 >At5g13570.1 68418.m01568 MutT/nudix family protein similar to mRNA-decapping enzyme [Homo sapiens] GI:23268269; contains Pfam profile PF00293: NUDIX domain Length = 373 Score = 57.6 bits (133), Expect = 6e-09 Identities = 28/81 (34%), Positives = 44/81 (54%) Frame = +3 Query: 258 KVYPCGIREFAAHIFQHVPQLREHVSSLDAVLDNWREYKQTVPTYGAIXXXXXXSHVLLV 437 K+ ++EF + +F LR +V+ +D + ++ YK VP GAI LLV Sbjct: 67 KLKSLSLKEFTSLLFNSCDVLRPYVTHIDDIFKDFTSYKCRVPVTGAIILDETYERCLLV 126 Query: 438 QSYWTKASWGFPKGKVNEDEE 500 + W +SW FP+GK ++DEE Sbjct: 127 KG-WKGSSWSFPRGKKSKDEE 146 Score = 54.0 bits (124), Expect = 7e-08 Identities = 20/38 (52%), Positives = 30/38 (78%) Frame = +1 Query: 121 DILDDLCSRFIINLPAEDRGNLVRICFQIELAHWFYLD 234 ++LDDLCSRF++N+P ED+ + RI F +E A+W+Y D Sbjct: 22 ELLDDLCSRFVLNVPEEDQQSFERILFLVEYAYWYYED 59 Score = 35.5 bits (78), Expect = 0.027 Identities = 14/20 (70%), Positives = 17/20 (85%) Frame = +2 Query: 509 CATREVLEETGFDISNLINK 568 CA REVLEETGFD+S L+ + Sbjct: 150 CAIREVLEETGFDVSKLLKR 169 >At5g02330.1 68418.m00156 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 656 Score = 29.1 bits (62), Expect = 2.4 Identities = 18/55 (32%), Positives = 25/55 (45%) Frame = -1 Query: 463 QEAFVQYDCXXXXXXXXXXXSIAPYVGTVCLYSLQLSKTASRLLTCSRSCGTCWK 299 +EAF Y C PYV +C + ++S T+S L + SCG C K Sbjct: 260 KEAFPTYVCLRCIFVAHRDCIYFPYVIKICRHQHRISFTSS-LPSGKWSCGVCRK 313 >At1g68760.1 68414.m07860 MutT/nudix family protein similar to SP|Q58549 ADP-ribose pyrophosphatase (EC 3.6.1.13) {Methanococcus jannaschii}; contains Pfam profile PF00293: NUDIX domain Length = 147 Score = 28.7 bits (61), Expect = 3.1 Identities = 13/24 (54%), Positives = 15/24 (62%) Frame = +2 Query: 506 ECATREVLEETGFDISNLINKNVT 577 ECA REV+EETG I + VT Sbjct: 51 ECAAREVMEETGLKIEKMKLLTVT 74 >At3g32260.1 68416.m04113 hypothetical protein Length = 309 Score = 28.3 bits (60), Expect = 4.1 Identities = 10/29 (34%), Positives = 18/29 (62%) Frame = +1 Query: 151 IINLPAEDRGNLVRICFQIELAHWFYLDY 237 +I++ E + N +RICF + W+Y+ Y Sbjct: 124 LIHVDCEVKTNRLRICFVEAMPKWYYIAY 152 >At1g30920.1 68414.m03782 F-box family protein contains F-box domain Pfam:PF00646 Length = 400 Score = 28.3 bits (60), Expect = 4.1 Identities = 12/21 (57%), Positives = 15/21 (71%) Frame = +1 Query: 85 MNSSHNKHSIPIDILDDLCSR 147 MNS N SIPID++ D+ SR Sbjct: 1 MNSEENTDSIPIDLILDILSR 21 >At4g12720.3 68417.m01998 MutT/nudix family protein similar to SP|P53370 Nucleoside diphosphate-linked moiety X motif 6 {Homo sapiens}; contains Pfam profile PF00293: NUDIX domain Length = 282 Score = 27.5 bits (58), Expect = 7.2 Identities = 16/56 (28%), Positives = 24/56 (42%), Gaps = 4/56 (7%) Frame = +3 Query: 393 GAIXXXXXXSHVLLVQS----YWTKASWGFPKGKVNEDEEPWNVLLERF*KRQGLI 548 GA+ VL+VQ + K W P G +NE E+ W + + G+I Sbjct: 107 GALVINKNTKEVLVVQERSGFFKDKNVWKLPTGVINEGEDIWTGVAREVEEETGII 162 >At4g12720.2 68417.m01997 MutT/nudix family protein similar to SP|P53370 Nucleoside diphosphate-linked moiety X motif 6 {Homo sapiens}; contains Pfam profile PF00293: NUDIX domain Length = 282 Score = 27.5 bits (58), Expect = 7.2 Identities = 16/56 (28%), Positives = 24/56 (42%), Gaps = 4/56 (7%) Frame = +3 Query: 393 GAIXXXXXXSHVLLVQS----YWTKASWGFPKGKVNEDEEPWNVLLERF*KRQGLI 548 GA+ VL+VQ + K W P G +NE E+ W + + G+I Sbjct: 107 GALVINKNTKEVLVVQERSGFFKDKNVWKLPTGVINEGEDIWTGVAREVEEETGII 162 >At4g12720.1 68417.m01996 MutT/nudix family protein similar to SP|P53370 Nucleoside diphosphate-linked moiety X motif 6 {Homo sapiens}; contains Pfam profile PF00293: NUDIX domain Length = 282 Score = 27.5 bits (58), Expect = 7.2 Identities = 16/56 (28%), Positives = 24/56 (42%), Gaps = 4/56 (7%) Frame = +3 Query: 393 GAIXXXXXXSHVLLVQS----YWTKASWGFPKGKVNEDEEPWNVLLERF*KRQGLI 548 GA+ VL+VQ + K W P G +NE E+ W + + G+I Sbjct: 107 GALVINKNTKEVLVVQERSGFFKDKNVWKLPTGVINEGEDIWTGVAREVEEETGII 162 >At3g07680.1 68416.m00921 emp24/gp25L/p24 family protein similar to SP|Q15363 Cop-coated vesicle membrane protein p24 precursor (p24A) {Homo sapiens}; contains Pfam profile PF01105: emp24/gp25L/p24 family Length = 208 Score = 27.5 bits (58), Expect = 7.2 Identities = 18/72 (25%), Positives = 35/72 (48%), Gaps = 2/72 (2%) Frame = +1 Query: 193 ICFQIELAHWFYLDYYCTDESRKYTPVV--LESLQHIFSNMSRNSENMLAV*MQF*ITGE 366 I F ++L H+ Y D + DE +TP++ + L+ N+ + A + I E Sbjct: 110 IDFDVQLGHFAYYDQHAKDE--HFTPLMEQISKLEEALYNIQFEQHWLEAQTDRQAIVNE 167 Query: 367 NINRLYLHTELY 402 N+++ +H L+ Sbjct: 168 NMSKRAVHKALF 179 >At2g38450.1 68415.m04722 expressed protein Length = 105 Score = 27.5 bits (58), Expect = 7.2 Identities = 17/70 (24%), Positives = 31/70 (44%), Gaps = 1/70 (1%) Frame = +1 Query: 55 TTDADMSSTIMNSSHNKHSIPI-DILDDLCSRFIINLPAEDRGNLVRICFQIELAHWFYL 231 ++D D SST S + +P+ ++ D R+ + E + I Q+ L +Y Sbjct: 3 SSDKDSSSTTTTSETTRRFVPLSSVVSDCAKRWFKDTLEEAKAG--NITMQVLLGQMYYS 60 Query: 232 DYYCTDESRK 261 Y ++RK Sbjct: 61 GYGVPKDARK 70 >At1g10930.1 68414.m01255 DNA helicase (RECQl4A) nearly identical to DNA Helicase [Arabidopsis thaliana] GI:11121449 Length = 1188 Score = 27.5 bits (58), Expect = 7.2 Identities = 14/45 (31%), Positives = 21/45 (46%) Frame = -1 Query: 328 CSRSCGTCWKICAANSLIPQGYTFLIRQCSNNPNKTNEPIRSGSI 194 C ++C C C++ SLI + T + RQ +T E S I Sbjct: 851 CKKTCDNC---CSSQSLIDKDVTLITRQLVELVKQTGERFSSAHI 892 >At1g03520.1 68414.m00333 glycosyltransferase family 14 protein / core-2/I-branching enzyme family protein contains Pfam profile PF02485: Core-2/I-Branching enzyme Length = 447 Score = 27.5 bits (58), Expect = 7.2 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = +1 Query: 208 ELAHWFYLDYYCTDESRKYTPVVLESLQHIFSNMSRN 318 E HW D++ + Y V + L ++FSN+SRN Sbjct: 193 ESLHW---DWFLNLSASDYPLVTQDDLLYVFSNLSRN 226 >At5g48910.1 68418.m06051 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 646 Score = 27.1 bits (57), Expect = 9.5 Identities = 13/36 (36%), Positives = 23/36 (63%) Frame = +3 Query: 270 CGIREFAAHIFQHVPQLREHVSSLDAVLDNWREYKQ 377 CGI E A H+F+ +P RE+V + A+++ + + Q Sbjct: 322 CGIIEKAIHVFERLP--RENVITWSAMINGFAIHGQ 355 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,327,349 Number of Sequences: 28952 Number of extensions: 249891 Number of successful extensions: 648 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 629 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 647 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1190791976 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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