BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesS0130 (698 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 161 9e-41 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 97 3e-21 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 96 5e-21 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 77 2e-15 SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 28 1.1 SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Sc... 28 1.5 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 27 2.6 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 3.4 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 4.5 SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 s... 26 4.5 SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|ch... 26 6.0 SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase Ubp4|Schizos... 26 6.0 SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|ch... 26 6.0 SPBC18A7.01 ||SPBC4F6.19c|X-Pro dipeptidase |Schizosaccharomyces... 25 7.9 SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomy... 25 7.9 SPAC24C9.14 |otu1|mug141|ubiquitin-specific protease |Schizosacc... 25 7.9 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 161 bits (391), Expect = 9e-41 Identities = 82/144 (56%), Positives = 97/144 (67%) Frame = +2 Query: 257 RINVYYNEASGGKYVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGH 436 R+NVY+NEA+GGKYVPRAV+VDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGH Sbjct: 46 RLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGH 105 Query: 437 YTEGAELVDSVLDVVRKEAEGCDCCRVFS*LILWEEVLDLVWGLF*FPKSVKSIQIRIMN 616 YTEGAEL D+VLDVVR+EAE CD + F G K + R+M Sbjct: 106 YTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMA 165 Query: 617 TFSVVPSXKGFPITVGRSHINATL 688 TFSV P+ K V NATL Sbjct: 166 TFSVAPAPKSSDTVV--EPYNATL 187 Score = 74.5 bits (175), Expect = 1e-14 Identities = 30/43 (69%), Positives = 35/43 (81%) Frame = +3 Query: 123 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQ 251 MREIVHIQAGQCGNQ+GA FW I+DEHG+D+ G Y G S+ Q Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQ 43 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 96.7 bits (230), Expect = 3e-21 Identities = 38/80 (47%), Positives = 56/80 (70%) Frame = +2 Query: 263 NVYYNEASGGKYVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYT 442 + +++E GKYVPR++ VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT Sbjct: 50 STFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYT 109 Query: 443 EGAELVDSVLDVVRKEAEGC 502 G ELVD V D +R+ A+ C Sbjct: 110 VGKELVDEVTDKIRRIADNC 129 Score = 40.7 bits (91), Expect = 2e-04 Identities = 18/34 (52%), Positives = 20/34 (58%) Frame = +3 Query: 123 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATG 224 MREI+ I GQ G QIG WE+ EHGI G Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNG 34 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 95.9 bits (228), Expect = 5e-21 Identities = 37/78 (47%), Positives = 57/78 (73%) Frame = +2 Query: 269 YYNEASGGKYVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEG 448 +++E GK+VPR++ VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G Sbjct: 56 FFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVG 115 Query: 449 AELVDSVLDVVRKEAEGC 502 E++DSVL+ +R+ A+ C Sbjct: 116 KEMIDSVLERIRRMADNC 133 Score = 41.1 bits (92), Expect = 1e-04 Identities = 19/47 (40%), Positives = 27/47 (57%) Frame = +3 Query: 123 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLNAS 263 MRE++ + GQ G QIG WE+ EHGI G + +S++ N S Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNS 47 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 77.4 bits (182), Expect = 2e-15 Identities = 38/129 (29%), Positives = 73/129 (56%), Gaps = 2/129 (1%) Frame = +2 Query: 257 RINVYYNEASGGKYVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAK 430 R +V++ ++ +Y+PRA+++DLEP ++++ S +G ++ P+N + ++ GAGNNWA Sbjct: 47 RKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWAN 106 Query: 431 GHYTEGAELVDSVLDVVRKEAEGCDCCRVFS*LILWEEVLDLVWGLF*FPKSVKSIQIRI 610 G Y+ + + ++D++ +EA+G D FS L G F + +I Sbjct: 107 G-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKI 165 Query: 611 MNTFSVVPS 637 + T+SV P+ Sbjct: 166 IQTYSVFPN 174 Score = 50.4 bits (115), Expect = 2e-07 Identities = 20/33 (60%), Positives = 26/33 (78%) Frame = +3 Query: 126 REIVHIQAGQCGNQIGAKFWEVISDEHGIDATG 224 REI+ +QAGQCGNQIG++FW+ + EHGI G Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDG 35 >SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 28.3 bits (60), Expect = 1.1 Identities = 14/32 (43%), Positives = 17/32 (53%) Frame = -3 Query: 414 PAPDCPKTKLSGRKICPNGPERTESMVPGSRS 319 P+ PK L R I PNGPE + + GS S Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51 >SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1458 Score = 27.9 bits (59), Expect = 1.5 Identities = 17/58 (29%), Positives = 27/58 (46%) Frame = -1 Query: 287 LKLRYSRH*CVKLQVGVAAVSTRGVNAVLVRDYFPELCSDLIPALTRLDVDDFPHDCT 114 ++L SR C + G+ + V + +Y P S+ + + RLDVD F H T Sbjct: 599 VRLGISRKRCERYPFGILCIIFT-VLEIAAEEYSPNWESEELRLVNRLDVDSFLHPKT 655 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 27.1 bits (57), Expect = 2.6 Identities = 15/62 (24%), Positives = 27/62 (43%) Frame = -2 Query: 529 ELAENPATIAAFCFFSNYIQYRINQFSPLCVMSFRPVIPRPRLSEDEVVRSEDLPKRPGT 350 +L P AA+ +S++ + FSP + P + P ++ V LP+ P + Sbjct: 103 QLPNEPQNSAAYTTYSSFPNALFDDFSPNNPLDTDPFLTSPGNKQNTVDSFRPLPETPVS 162 Query: 349 DG 344 G Sbjct: 163 PG 164 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 26.6 bits (56), Expect = 3.4 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +2 Query: 356 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 457 GP+G +F P F+F +G NW+ Y E A L Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 26.2 bits (55), Expect = 4.5 Identities = 15/43 (34%), Positives = 22/43 (51%) Frame = +2 Query: 425 AKGHYTEGAELVDSVLDVVRKEAEGCDCCRVFS*LILWEEVLD 553 A+GH G ELV + D +RK++E V I+ E L+ Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALEVCPDRIVCNEFLE 225 >SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 subunit Alp6|Schizosaccharomyces pombe|chr 2|||Manual Length = 821 Score = 26.2 bits (55), Expect = 4.5 Identities = 10/35 (28%), Positives = 18/35 (51%) Frame = -1 Query: 191 YFPELCSDLIPALTRLDVDDFPHDCTKNLEPPESN 87 +F E+ + P +LD+DD + + P E+N Sbjct: 36 FFQEIIHSISPDTFQLDIDDILYKIYSKIPPEENN 70 >SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 25.8 bits (54), Expect = 6.0 Identities = 17/53 (32%), Positives = 26/53 (49%) Frame = +1 Query: 88 FDSGGSKFLVQS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPT 246 F S S S +STS RV++ ++ SSG + T+ + V +AT T Sbjct: 165 FVSPSSSSSSSSSAATSTSTRVSSSAKASTSSGAIAYTTKCVVVPVTASATAT 217 >SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase Ubp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 25.8 bits (54), Expect = 6.0 Identities = 11/34 (32%), Positives = 17/34 (50%) Frame = +1 Query: 583 IREEYPDSYYEYVFRGTKXKRFSDNCG*KPYKRN 684 +RE++ SY + R + + F D C KRN Sbjct: 403 LREDFSSSYILFYKRSSLLEEFEDKCPKMTLKRN 436 >SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 25.8 bits (54), Expect = 6.0 Identities = 17/53 (32%), Positives = 26/53 (49%) Frame = +1 Query: 88 FDSGGSKFLVQS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPT 246 F S S S +STS RV++ ++ SSG + T+ + V +AT T Sbjct: 165 FVSPSSSSSSSSSAATSTSTRVSSSAKASTSSGAIAYTTKCVVVPVTASATAT 217 >SPBC18A7.01 ||SPBC4F6.19c|X-Pro dipeptidase |Schizosaccharomyces pombe|chr 2|||Manual Length = 451 Score = 25.4 bits (53), Expect = 7.9 Identities = 9/26 (34%), Positives = 14/26 (53%) Frame = -2 Query: 622 KRIHNTNLDTLHGFWKSKESPYQIQY 545 K + N+D + W E+PYQ+ Y Sbjct: 154 KELVGKNIDAKYITWDEDENPYQVLY 179 >SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 25.4 bits (53), Expect = 7.9 Identities = 7/21 (33%), Positives = 14/21 (66%) Frame = -2 Query: 148 AWMWTISLMIALKIWNPPNRI 86 AW+W ++LM+ + PN++ Sbjct: 458 AWLWFMALMLLFPSYQNPNKV 478 >SPAC24C9.14 |otu1|mug141|ubiquitin-specific protease |Schizosaccharomyces pombe|chr 1|||Manual Length = 329 Score = 25.4 bits (53), Expect = 7.9 Identities = 9/21 (42%), Positives = 13/21 (61%) Frame = -1 Query: 464 NQPVQPPLCNVLSPSYSPPQT 402 N+P +PP+ N + PPQT Sbjct: 86 NEPAKPPIPNAATKPTFPPQT 106 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,979,194 Number of Sequences: 5004 Number of extensions: 62829 Number of successful extensions: 202 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 186 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 200 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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