Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= tesS0111
(598 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 132 4e-32
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 83 4e-17
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 81 1e-16
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 67 2e-12
SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharo... 29 0.68
SPCC594.02c |||conserved fungal protein|Schizosaccharomyces pomb... 26 3.6
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 26 4.8
SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizo... 25 6.3
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 132 bits (319), Expect = 4e-32
Identities = 62/91 (68%), Positives = 71/91 (78%), Gaps = 1/91 (1%)
Frame = +3
Query: 249 PR-ILVXLEPGTMDSVRSGPFGQIFRPDNFXFXQXGAGNNWAKGHYTEGAXLVDSVIDVV 425
PR +LV LEPGTMD+V+SG FG +FRPDN + Q GAGN WAKGHYTEGA L D+V+DVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 426 RKEAESCDCLQGFQLTHSLXRXHRFXYGTLL 518
R+EAE+CD LQGFQLTHSL GTLL
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLL 151
Score = 105 bits (252), Expect = 5e-24
Identities = 44/62 (70%), Positives = 51/62 (82%)
Frame = +1
Query: 67 MREIVHIQAGQCGNQIGAKFWEIISDXHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 246
MREIVHIQAGQCGNQ+GA FW I+D HG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 247 XR 252
R
Sbjct: 61 PR 62
Score = 50.0 bits (114), Expect = 3e-07
Identities = 22/34 (64%), Positives = 26/34 (76%)
Frame = +1
Query: 484 GGXTGSCMAPSXISKIREEYPDRIMNTYSVVPSP 585
GG TGS M +SKIREEYPDR+M T+SV P+P
Sbjct: 140 GGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAP 173
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 82.6 bits (195), Expect = 4e-17
Identities = 40/91 (43%), Positives = 54/91 (59%), Gaps = 1/91 (1%)
Frame = +3
Query: 249 PR-ILVXLEPGTMDSVRSGPFGQIFRPDNFXFXQXGAGNNWAKGHYTEGAXLVDSVIDVV 425
PR I V LEP +D VR+GP+ +F P+ + A NN+A+GHYT G LVD V D +
Sbjct: 63 PRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKI 122
Query: 426 RKEAESCDCLQGFQLTHSLXRXHRFXYGTLL 518
R+ A++C LQGF + HS +G LL
Sbjct: 123 RRIADNCSGLQGFLVFHSFGGGTGSGFGALL 153
Score = 49.6 bits (113), Expect = 3e-07
Identities = 25/64 (39%), Positives = 34/64 (53%), Gaps = 2/64 (3%)
Frame = +1
Query: 67 MREIVHIQAGQCGNQIGAKFWEIISDXHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 240
MREI+ I GQ G QIG WE+ HGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 241 YVXR 252
YV R
Sbjct: 61 YVPR 64
Score = 25.0 bits (52), Expect = 8.4
Identities = 11/34 (32%), Positives = 17/34 (50%)
Frame = +1
Query: 484 GGXTGSCMAPSXISKIREEYPDRIMNTYSVVPSP 585
GG TGS + ++ EY + +SV P+P
Sbjct: 142 GGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAP 175
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 81.0 bits (191), Expect = 1e-16
Identities = 38/91 (41%), Positives = 55/91 (60%), Gaps = 1/91 (1%)
Frame = +3
Query: 249 PR-ILVXLEPGTMDSVRSGPFGQIFRPDNFXFXQXGAGNNWAKGHYTEGAXLVDSVIDVV 425
PR I V LEP +D VR+GP+ +F P+ + A NN+A+GHYT G ++DSV++ +
Sbjct: 67 PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI 126
Query: 426 RKEAESCDCLQGFQLTHSLXRXHRFXYGTLL 518
R+ A++C LQGF + HS G LL
Sbjct: 127 RRMADNCSGLQGFLVFHSFGGGTGSGLGALL 157
Score = 43.6 bits (98), Expect = 2e-05
Identities = 22/68 (32%), Positives = 33/68 (48%), Gaps = 6/68 (8%)
Frame = +1
Query: 67 MREIVHIQAGQCGNQIGAKFWEIISDXHGIDPTG------AYHGDSDLQLERINVYYNEA 228
MRE++ + GQ G QIG WE+ HGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 229 SGGKYVXR 252
GK+V R
Sbjct: 61 GQGKFVPR 68
Score = 25.4 bits (53), Expect = 6.3
Identities = 11/34 (32%), Positives = 18/34 (52%)
Frame = +1
Query: 484 GGXTGSCMAPSXISKIREEYPDRIMNTYSVVPSP 585
GG TGS + + ++ EY + +SV P+P
Sbjct: 146 GGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 66.9 bits (156), Expect = 2e-12
Identities = 30/81 (37%), Positives = 51/81 (62%), Gaps = 3/81 (3%)
Frame = +3
Query: 249 PR-ILVXLEPGTMDSVRSGPFGQIFRPDNFXFXQXG--AGNNWAKGHYTEGAXLVDSVID 419
PR IL+ LEP ++++ S +G ++ P+N + G AGNNWA G Y+ + + ++D
Sbjct: 62 PRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMD 120
Query: 420 VVRKEAESCDCLQGFQLTHSL 482
++ +EA+ D L+GF L HS+
Sbjct: 121 MIDREADGSDSLEGFSLLHSI 141
Score = 59.3 bits (137), Expect = 4e-10
Identities = 24/61 (39%), Positives = 40/61 (65%)
Frame = +1
Query: 70 REIVHIQAGQCGNQIGAKFWEIISDXHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVX 249
REI+ +QAGQCGNQIG++FW+ + HGI P G + ++R +V++ ++ +Y+
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 250 R 252
R
Sbjct: 63 R 63
Score = 34.7 bits (76), Expect = 0.010
Identities = 16/41 (39%), Positives = 26/41 (63%)
Frame = +1
Query: 472 HTRSGGXTGSCMAPSXISKIREEYPDRIMNTYSVVPSP*SV 594
H+ +GG TGS + + ++ + YP +I+ TYSV P+ SV
Sbjct: 139 HSIAGG-TGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSV 178
>SPCC63.04 |mok14||alpha-1,3-glucan synthase
Mok14|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1369
Score = 28.7 bits (61), Expect = 0.68
Identities = 21/73 (28%), Positives = 35/73 (47%), Gaps = 4/73 (5%)
Frame = -3
Query: 428 SNDVDN*IDEXSTLRVVSLGPVVAGAXLSEXEVVRTEDLSERSRADR----VHGAGLQXD 261
+N +DN DE + + S+ ++ + LSE + ++ SR D +G ++ D
Sbjct: 783 ANSMDNDSDEDNYEQAESIISSLSSSALSELSYISESSMNIGSRLDERFIDANGVAIR-D 841
Query: 260 EDARXTYLPPEAS 222
A TYL PE S
Sbjct: 842 FSAELTYLTPENS 854
>SPCC594.02c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 489
Score = 26.2 bits (55), Expect = 3.6
Identities = 12/31 (38%), Positives = 19/31 (61%), Gaps = 1/31 (3%)
Frame = +3
Query: 456 QGFQLTHSLXRXHRFXYGTLLHL-QNQRRVP 545
+GF LT + H + YG LH+ +NQ+ +P
Sbjct: 230 EGFVLTPWIAFLHMYMYGHYLHVAKNQKSLP 260
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 25.8 bits (54), Expect = 4.8
Identities = 10/34 (29%), Positives = 21/34 (61%)
Frame = +1
Query: 106 NQIGAKFWEIISDXHGIDPTGAYHGDSDLQLERI 207
N++G E++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPCC576.10c |rpt3||19S proteasome regulatory subunit
Rpt3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 389
Score = 25.4 bits (53), Expect = 6.3
Identities = 18/88 (20%), Positives = 34/88 (38%)
Frame = -3
Query: 389 LRVVSLGPVVAGAXLSEXEVVRTEDLSERSRADRVHGAGLQXDEDARXTYLPPEASL*YT 210
L + + G+ VVR +R LQ +A LPPEA +
Sbjct: 62 LEAIDQNTAIVGSTTGSNYVVRILSTLDRELLKPSASVALQRHSNALVDILPPEADSSIS 121
Query: 209 LMRSNCKSESPW*APVGSMPXSSEMISQ 126
++R + + + + A VG + + + +
Sbjct: 122 MLRPDERPDVSY-ADVGGLDVQKQEVRE 148
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,022,337
Number of Sequences: 5004
Number of extensions: 32134
Number of successful extensions: 76
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 64
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 74
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 260219058
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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