BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesS0079 (449 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 107 6e-25 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 72 4e-14 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 71 8e-14 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 52 4e-08 SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 28 0.58 SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 s... 26 2.3 SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|ch... 26 3.1 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 26 3.1 SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|ch... 26 3.1 SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomy... 25 4.1 SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe... 25 5.4 SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Sc... 25 7.1 SPAC1834.02 |aro1||pentafunctional aromatic polypeptide Aro1 |Sc... 25 7.1 SPCC16A11.01 ||SPCC63.15|conserved fungal protein|Schizosaccharo... 24 9.4 SPBC2G2.14 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 24 9.4 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 107 bits (258), Expect = 6e-25 Identities = 45/61 (73%), Positives = 55/61 (90%) Frame = +2 Query: 254 HVYYNEASGGKYVPRAVMVDLXPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNLAKGHYT 433 +VY+NEA+GGKYVPRAV+VDL PGTMD+V+SG FG +FRPDN ++GQSGAGN AKGHYT Sbjct: 48 NVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYT 107 Query: 434 D 436 + Sbjct: 108 E 108 Score = 78.2 bits (184), Expect = 5e-16 Identities = 32/47 (68%), Positives = 38/47 (80%) Frame = +3 Query: 114 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLERI 254 MREIVHIQAGQCGNQ+GA FW I+DEHG+D+ G Y G S+ Q ER+ Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERL 47 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 72.1 bits (169), Expect = 4e-14 Identities = 27/58 (46%), Positives = 40/58 (68%) Frame = +2 Query: 260 YYNEASGGKYVPRAVMVDLXPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNLAKGHYT 433 +++E GKYVPR++ VDL P +D VR+GP+ +F P+ + G+ A NN A+GHYT Sbjct: 52 FFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYT 109 Score = 40.7 bits (91), Expect = 1e-04 Identities = 18/34 (52%), Positives = 20/34 (58%) Frame = +3 Query: 114 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATG 215 MREI+ I GQ G QIG WE+ EHGI G Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNG 34 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 70.9 bits (166), Expect = 8e-14 Identities = 27/58 (46%), Positives = 40/58 (68%) Frame = +2 Query: 260 YYNEASGGKYVPRAVMVDLXPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNLAKGHYT 433 +++E GK+VPR++ VDL P +D VR+GP+ +F P+ V G+ A NN A+GHYT Sbjct: 56 FFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYT 113 Score = 38.7 bits (86), Expect = 4e-04 Identities = 17/42 (40%), Positives = 25/42 (59%) Frame = +3 Query: 114 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDL 239 MRE++ + GQ G QIG WE+ EHGI G + +S++ Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEV 42 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 52.0 bits (119), Expect = 4e-08 Identities = 19/59 (32%), Positives = 40/59 (67%), Gaps = 2/59 (3%) Frame = +2 Query: 257 VYYNEASGGKYVPRAVMVDLXPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNLAKGH 427 V++ ++ +Y+PRA+++DL P ++++ S +G ++ P+N + ++ GAGNN A G+ Sbjct: 50 VFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANGY 108 Score = 50.4 bits (115), Expect = 1e-07 Identities = 20/33 (60%), Positives = 26/33 (78%) Frame = +3 Query: 117 REIVHIQAGQCGNQIGAKFWEVISDEHGIDATG 215 REI+ +QAGQCGNQIG++FW+ + EHGI G Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDG 35 >SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 28.3 bits (60), Expect = 0.58 Identities = 14/32 (43%), Positives = 17/32 (53%) Frame = -3 Query: 405 PAPDCPKTKLSGRKICPNGPERTESMVPGSRS 310 P+ PK L R I PNGPE + + GS S Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51 >SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 subunit Alp6|Schizosaccharomyces pombe|chr 2|||Manual Length = 821 Score = 26.2 bits (55), Expect = 2.3 Identities = 10/35 (28%), Positives = 18/35 (51%) Frame = -1 Query: 182 YFPELCSDLIPALTRLDVDDFPHDCTKNLEPPESN 78 +F E+ + P +LD+DD + + P E+N Sbjct: 36 FFQEIIHSISPDTFQLDIDDILYKIYSKIPPEENN 70 >SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 25.8 bits (54), Expect = 3.1 Identities = 17/53 (32%), Positives = 26/53 (49%) Frame = +1 Query: 79 FDSGGSKFLVQS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPT 237 F S S S +STS RV++ ++ SSG + T+ + V +AT T Sbjct: 165 FVSPSSSSSSSSSAATSTSTRVSSSAKASTSSGAIAYTTKCVVVPVTASATAT 217 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 25.8 bits (54), Expect = 3.1 Identities = 8/18 (44%), Positives = 12/18 (66%) Frame = +2 Query: 347 GPFGQIFRPDNFVFGQSG 400 GP+G +F P F+F +G Sbjct: 157 GPYGTMFLPQQFIFDSNG 174 >SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 25.8 bits (54), Expect = 3.1 Identities = 17/53 (32%), Positives = 26/53 (49%) Frame = +1 Query: 79 FDSGGSKFLVQS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPT 237 F S S S +STS RV++ ++ SSG + T+ + V +AT T Sbjct: 165 FVSPSSSSSSSSSAATSTSTRVSSSAKASTSSGAIAYTTKCVVVPVTASATAT 217 >SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 25.4 bits (53), Expect = 4.1 Identities = 7/21 (33%), Positives = 14/21 (66%) Frame = -2 Query: 139 AWMWTISLMIALKIWNPPNRI 77 AW+W ++LM+ + PN++ Sbjct: 458 AWLWFMALMLLFPSYQNPNKV 478 >SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 317 Score = 25.0 bits (52), Expect = 5.4 Identities = 17/64 (26%), Positives = 33/64 (51%) Frame = +1 Query: 109 QS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPTCSLNASCLL*RSFRGXV 288 +S S +S ++ +S SS S++S++ + ++A+P+ S ++S SF Sbjct: 171 KSSSSSKSSSSSSSSSKSSSSSSSSSKSSSSSSSSSKSSASPSSSKSSSKFSSSSFITST 230 Query: 289 RPAS 300 PAS Sbjct: 231 TPAS 234 >SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1458 Score = 24.6 bits (51), Expect = 7.1 Identities = 16/58 (27%), Positives = 26/58 (44%) Frame = -1 Query: 278 LKLRYSRHDAFKLQVGVAAVSTRGVNAVLVRDYFPELCSDLIPALTRLDVDDFPHDCT 105 ++L SR + G+ + V + +Y P S+ + + RLDVD F H T Sbjct: 599 VRLGISRKRCERYPFGILCIIFT-VLEIAAEEYSPNWESEELRLVNRLDVDSFLHPKT 655 >SPAC1834.02 |aro1||pentafunctional aromatic polypeptide Aro1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1573 Score = 24.6 bits (51), Expect = 7.1 Identities = 14/48 (29%), Positives = 20/48 (41%) Frame = +3 Query: 93 FQIFSAIMREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSD 236 F + S I I C + +W+V+ G+ TGA S SD Sbjct: 807 FSVLSLICPSRTLIIDKACVEKTWPYWWDVLHQSFGVKLTGATSVASD 854 >SPCC16A11.01 ||SPCC63.15|conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 328 Score = 24.2 bits (50), Expect = 9.4 Identities = 9/25 (36%), Positives = 15/25 (60%) Frame = -2 Query: 154 FPH*PAWMWTISLMIALKIWNPPNR 80 FP MWTI L++ L +W+ ++ Sbjct: 25 FPLVSFLMWTIGLIVLLGLWSTQDK 49 >SPBC2G2.14 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 533 Score = 24.2 bits (50), Expect = 9.4 Identities = 10/40 (25%), Positives = 18/40 (45%) Frame = -1 Query: 422 LSPSYSPPQTVRRRSCPVGRFAQTARNGRSPWFQXQGPPS 303 LSPS++PP V+ F ++ + W + P+ Sbjct: 222 LSPSFAPPSNVKSPVQQHRSFVSSSARAKKNWGRQSNSPN 261 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,820,372 Number of Sequences: 5004 Number of extensions: 34658 Number of successful extensions: 100 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 96 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 100 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 166231220 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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